developed network protocol and speci cation of

... on the inhibition of xylanases by TAXI-type inhibitors Materials and methods Production and puri cation of xylanases and xylanase inhibitors TAXI-I (i.e a mixture of TAXI-IA and TAXI-IB) was purified ... and ˚ Asp11BSX Od2 (3.7 A), similarly to the BSXÆTAXI-IA complex Discussion The strength and speci city of inhibition of TAXI-Iand TAXI-II-type inhibitors differ strongly (Table 1) Analysis of ... optimum of results from a combination of an aspartate and a tryptophan residue Both our criteria on the strength and speci city of the inhibition, however, indicate an increased susceptibility of...

Ngày tải lên: 23/03/2014, 05:22

... characteristic (b ⁄ a)8-barrel catalytic A domain, a B domain between b strand and a helix 3, and a C-terminal domain consisting of a sandwich of two Greek key motifs In addition to these common structural ... affinity and low Vmax at low sucrose concentrations (Vmax1 and Km1) and low affinity and high(er) Vmax at high sucrose concentrations (Vmax2 and Km2) [13] In Table the kinetic data Table Kinetics of ... Detail of the structure of the Glu328Gln amylosucrase complexed with maltoheptaose (PDB code 1MW0), showing the positions of the mutated residues Asn387 in b strand 7, and Val389 in the first part of...

Ngày tải lên: 23/03/2014, 11:20

... attribute structure of TIMEX, [(eid signalID relType)] Moreover, as the speci cation of the values for the eventRelType and timeRelType attributes of EVENT and the relType attribute of TIMEX indicates, ... respectively And, of course, since relType is defined on TIMEX, not EVENT, it must repeat the speci cation of permissible values All these considerations suggest that these triplets of attributes ... intrasententially and in discourse; and it provides a semantics for underspecified temporal expressions, thereby allowing for a delayed interpretation Most of the details of this last component of TimeML...

Ngày tải lên: 30/03/2014, 16:20

... Digivance® Simulcast Networks New Network Architecture Reduce Network Costs and Improve Quality of Service Improving Grade of Service (GOS) While Reducing RF Channels Wireless ... to reduce network costs and improve quality of service In one urban market, a major national wireless service provider has improved network traffic loading and significantly boosted network RF ... urban LRCS network, the new architecture reduced by 30 percent or more of the total number of RF channels required on a multiple link transport system for a specified amount of user grade of service...

Ngày tải lên: 10/12/2013, 19:15

... E22K, E22Q and D23N), and the availability of a rapid and simple expression and puri cation protocol will facilitate large-scale investigations of the molecular determinants of aggregation and fibrillation ... assessment of the purity of the product or the correctness of the sequence In contrast, the puri cation protocol that we have developed is quick and efficient, and leads to the production of highly ... cost of the puri cation, and the require1276 ment for HPLC increases the length and complexity of the puri cation procedure Moreover, as with the studies by Lee et al [21] and Subramanian and...

Ngày tải lên: 18/02/2014, 13:20

... a-galactosidase speci cities [2] At least one half of the family is formed by ORFs coding for putative proteins of uncharacterized activity and speci city A striking feature of GH-57 is the sequence and ... TIM barrel) and two acidic residues, Glu123 and Asp214, which appear to define the catalytic centre of the enzyme Importantly, the distance between the pair of oxygen atoms of Glu123 and ˚ Asp214 ... basis of its catalytic activity, to provide new understanding concerning its bifunctionality and to establish links between this GH-57 amylopullulanase and other non pullulandegrading GH-57 and...

Ngày tải lên: 19/02/2014, 12:20

... presence of 2-amino-2-deoxygalactose and lysine The D configuration of GalA and GalN was determined by GLC of the acetylated (S)-2-butyl glycosides and the L configuration of lysine by GLC of the ... for GlcA and GalA and between H1 and H2–H4 for both GalNAc residues (GalNAcI and GalNAcII) The signals for H5 and H6 of GalNAcI were assigned by H4/H5 correlation in the ROESY spectrum and H5/H6 ... reaction with LPS of both P mirabilis G1 and S1959, whereas LPS of P mirabilis O28 and R14 reacted markedly weaker (Fig 4) The speci city of the crossreactions was confirmed by inhibition of the reaction...

Ngày tải lên: 21/02/2014, 15:20

... with [32P]ATP[aP]) and the photoregulatory efficiency of transcription (a) was Fig Sequences of photoresponsive T7 promoters and the structures of Azo and its derivatives (M-azo and DM-azo) used ... thermal stability of the cis form between Azo and DM-azo (A) Illustration of the reversible photoisomerization of Azo and thermal isomerization of cis-Azo (B) Quantitative calculation of cis-form content ... Photoregulation of DNA transcription Table Proportions of the four promoter species (trans–trans, trans–cis, cis–trans and cis–cis isomers) of two different azobenzene-modified DNAs involving one DM-azo and...

Ngày tải lên: 15/03/2014, 10:20

... determined The effects of the replacement of C74, C105 and C265 with a serine residue on the expression and complementation of the mutant phenotype of the gas1 null mutant of S cerevisiae were ... features of proteins of the GH72 family and demonstrate that the most N-terminal highly conserved cysteine is crucial for the folding and maturation of Gas1p Materials and methods Strains and growth ... consisting of a structure that contains both a-helices and b-sheets, and that the latter ones predominate Quanti cation of disulphides and free sulphydryl groups present in Gas1p Proteins of Family...

Ngày tải lên: 16/03/2014, 18:20

... Phosphorylation of this protein on serine residue was described in E coli [22] and C glutamicum [15] The significance of its modi cation and nature of modifying enzyme remains unclear One of the phosphoproteins ... Positions and molecular mass (kDa) of protein standards are indicated on the left The arrows indicate the position of phosphorylated rStkP and rGlmM 21 Fig Image of the 2D gel electrophoresis of phosphoproteins ... extract (lanes and 5), cytosolic fraction (lanes and 6) and membrane fraction (3 and 7) of S pneumoniae strains Purified recombinant StkP was used as a control (lane 4) Arrows indicate bands corresponding...

Ngày tải lên: 16/03/2014, 18:20

... one strain of P myxofaciens, one strain of P hauseri and Providencia stuartii O18, only LPS of P vulgaris OX2 and 5539 O-polysaccharides of Proteus genomospecies Fig EIA binding curves of Proteus ... O-polysaccharides of Proteus [16] Studies of the O-polysaccharides of Proteus genomospecies and and the comparison of the corresponding 13C NMR spectra indicated that they are identical to those of P vulgaris ... O-polysaccharides of Proteus genomospecies two other recognized medically important species, P penneri and P hauseri, nor Proteus genomospecies 4, and [6–9] Previously, as a result of the chemical and serological...

Ngày tải lên: 16/03/2014, 23:20

... the anticipated sequence of subunit C on the basis of their mass value and the speci city of the enzyme, leading to the complete veri cation of the amino acid sequence of subunit C (GenBank accession ... in Materials and methods Quantitative analysis of bound FAD yielded the value of 1.1–1.2 mol of FAD per mole of protein The iron content of Tomo F was 1.8–2.1 molÆmol)1 of protein, and the acid-labile ... using mixtures of Tomo H, F, C and D, and changing the concentration of each single component Figure shows the effects on the rate of reaction of increasing ratios of Tomo F, Tomo C and Tomo D with...

Ngày tải lên: 17/03/2014, 10:20

... 50 mL of buffer A and developed by a 500-mL linear gradient of to 500 mM NaCl in buffer A Fractions containing CoA-transferase activity were pooled and adjusted to a ®nal concentration of M (NH4)2SO4 ... outer layers of a helices followed by one layer of b sheets and an inner layer of a helices This arrangement is found in both subunits of the protein It is remarkable that the outer layer of a helices ... respectively) of the b subunit of glutaconate CoA-transferase protrude into a cleft on the surface of the a subunit and are involved in mediation of subunit interactions Therefore, the lack of this...

Ngày tải lên: 17/03/2014, 17:20

... value of 3.5, an optimal pH for ABTS of 2.7 and for syringaldazine of 5–5.5 The properties for Lcc2 are quite different with a pI value of 6.2–6.8, optimal pH for ABTS of and for syringaldazine of ... laccases in the ancestors of these species that have been maintained during the speciation and diversi cation of the Homobasidiomycete fungi Evidence for different functions of the various laccases ... in bacteria and their classi cation and function are still controversial The first report of a bacterial laccase was from the Gram-negative soil bacterium Azospirillum lipoferum [33] and the enzyme...

Ngày tải lên: 23/03/2014, 11:20

... Institute of Iceland and 50 specimens were caught randomly After killing the fish by a concussion of the brain by striking of the cranium, mucus samples were collected by scraping the skin and then ... International Cooperation in Research and Higher Education (STINT), The Swedish Research Council and AVS R & D Fund of Ministry of Fisheries in Iceland MALDI MS Aliquots of HPLC fractions were mixed (1 ... conserved [22,23], cationic, bactericidal polypeptides from the skin mucus of cod, i.e histone H2B and three 60S ribosomal proteins, L40, L36A and L35 As seen (Figs 2A and 3A) by the number of antimicrobial...

Ngày tải lên: 23/03/2014, 15:21

... Quantitative PCR indicated highest levels of P450 4X1 mRNA in brain regions and skin The oxidation of anandamide (and a stable analog of anandamide and D9-tetrahydrocannabinol), although slow, ... rates of oxidation of a number of other arachidonic acid derivatives, neurosteroids (e.g dopamine and tyramine) and common drugs (e.g loratadine and clotrimazole) were below the limits of detection ... ethanolamide), and P450 3A4 (in the liver and small intestine) has been reported to form all four epoxides (EETs) of anandamide [11] Administration of anandamide to rats increased the levels of P450...

Ngày tải lên: 30/03/2014, 04:20

... estimate of Q, and (2) the number of sources P to be separated is known to determine the number of columns of US spanned by the integer-translated and dilated copies φ j,k = j j/2 φ(2· − k) of a ... row space of X j and that of X using the whitening-rotation factorization of A discussed above Speci cally, if W ( j) spans the null space of the product HT S, the corresponding rows of HT Sj ... the subbands based on the magnitude of their eigenvalues relative to the 0th subband eigenvalue (4) For each of the rank-ordered subbands, calculate the distance between each eigenvector and the...

Ngày tải lên: 22/06/2014, 23:20

... tracking and classi cation, ” in Signal and Data Processing of Small Targets, vol 4728 of Proceedings SPIE, pp 439–449, Orlando, Fla, USA, April 2002 S Challa and G Pulford, “Joint target tracking and ... distribution of the st and xτ processes and the times of the sojourns, τ1:tk , up to the time of the kth measurement, τk , as A weight is then assigned according to the principle of importance ... extends the existing set of sojourn times and the st process with samples of the sojourns that took place between the previous and the current measurements: (10) and estimates of expectations calculated...

Ngày tải lên: 23/06/2014, 01:20

... indecomposable if and only if A and ∅ are the only subSOMAs of A The disjoint union of subSOMAs of A is a subSOMA, and it is easy to see that if B and C are subSOMAs of A, then B ∩ C is a subSOMA if and only ... size of v Vertices v and w are adjacent in ∆ if and only if v = w and v ∪ w is a clique of Γ Now let N be a subgroup of Aut(Γ) such that N normalizes H Then N permutes the H-orbits of vertices of ... cliques of ∆ of weight-sum 10k with k > 3, but there are exactly 22 N-orbit representatives of the cliques of ∆ of weight-sum 30 In addition, it turns out that the union of the elements of each of...

Ngày tải lên: 07/08/2014, 06:20

... biotopes of (a) FP (9 plots of 20) and (b) BV (11 plots of 20) – so the general speci cations of point (1) apply to bio- 186 topes (a) and (b) to describe the stability and variability of soil ... different method of statistical assessment of BF contrary to FP and BV The reason is a small size of FP and BV sets and slight invalidation of normality contrary to an optimal size of BF, when ANOVA ... facilitation of research plot diversi cation in FP and BV to determine that the soils of Fageta paupera are mostly the cause of large differences and that they are characterized by high heterogeneity of...

Ngày tải lên: 07/08/2014, 10:21