determination of antibody glycosylation by mass spectrometry

... 2 Mass spectra of digested flagellins of (a,d) ∆vioR; (b,e) ∆vioM; and (c,f) the WT of Pta 6605 Upper spectra (a–c) were of trypsin-digested flagellins by MALDI-TOF MS The average masses of ... for glycosylation of mVio in Pta 6605 by the mass spectrometry (MS) of flagellin glycan in the respective mutants Furthermore, characterization of the mVio-related genes and MS analyses of flagellin ... 2.2 Effects of Each Mutation of vioM and vioR on Flagellin Glycosylation in Pta 6605 To assess the effects of gene deletion, we analyzed flagellins in ∆vioR and ∆vioM mutants by mass spectrometry

Ngày tải lên: 04/12/2022, 10:35

... Optimization of a gas chromatography–mass spectrometry method using chemometric techniques for the determination of ezetimibe in human plasma Ebru UC ¸ AKT ¨ URK,∗Nuran ¨ OZALTIN Department of Analytical ... with 40 µ L of Trang 32.4 Preparation of derivatization reagentsβ -mercaptoethanol was added to it Working solution was prepared by dilution of 556 µ L of stock solution with 5 mL of MSTFA MSTFA/Imidazole ... prepared by serial dilution of stock solution of EZE with methanol Stock In order to prepare spiked plasma samples for the validation study, an appropriate amount of working standard solution of EZE

Ngày tải lên: 12/01/2022, 22:50

... article is part of the themed issue ‘Quantitative mass spectrometry’ 1 Introduction Gas chromatography-mass spectrometry (GC-MS) has been the gold standard for the identification of natural ingredients ... series of determinations will fall, if done by the same number of participants: Trang 7+l –l conc range lower LOQ upper LOQ 0 calibration range mean bias C5 Figure 2 Scheme of an accuracy profile ... advantage of the accuracy profile is the evaluation of the performances of a method over the entire validation range LOQ can be adapted on the basis of the tolerance interval defined by the analyses

Ngày tải lên: 04/12/2022, 10:36

... VON-AMPE HOÀ TAN CATOT XUNG VI PHÀN KHI CO MATRh ULTRATRACE DETERMINATION OF SELENIUM (IV) BY CATHODIC STRIPPINO VOLTAMMETRY IN THE PRESENCE OF RHODIUM T r a n ChiioTng H u y e n , Le T h i HvTcyng ... from the standard deviation of a low selenium concentration (0,05ppb) using deposition time of 2 minute The interference of some metals were investigated and eliminated by using ion-exchange resin ... 1.8 (HCl concentration of 10''^ M), 400ppb - 4ppm Rb(III) (depending on the Se(I\0 concentration), and a deposition potential of -0.25V (vs.Ag/AgCl) The 3 detection limit of 2.2ppt Se(IV) was caculated

Ngày tải lên: 12/03/2015, 14:47

... the determination of the PM2,5 mass fraction of suspended particulate matter Qualité de l'air ambiant - Méthode de mesurage gravimétrique de référence pour la détermination de la fraction massique ... This European Standard exists in three official versions (English, French, German) A version in any other language made by translationunder the responsibility of a CEN member into its own language ... GravimetrischesStandardmessverfahren für die Bestimmung der PM2,5- Massenfraktion des Schwebstaubs This European Standard was approved by CEN on 22 July 2005 CEN members are bound to comply with the

Ngày tải lên: 18/02/2021, 15:24

... Theaimofthisstudywasthereforetoassesstheapplicabilityof SFC,coupledtoahigh-resolutionmassspectrometer,inthefieldof metabolomicsbyusinganextendedsetofmetabolites TheSigma MassSpectrometryMetaboliteLibraryofStandards(MSMLS), com-posed of nearly ... Oncethesethreebehaviorswereidentified,theMS/MSspectra generatedbytheentiresetof49metaboliteswereassessed.63%of thecompoundswerecharacterizedby anabsenceofinterferences in anybiological matrices Out of the remaining 37%, 21% suffer ... SigmaMass SpectrometryMetabolite Libraryof Standardscontaining597metabolites,underSFC–HRMSconditionswasperformed.Atotaldetectability ofthecommerciallibraryupto66%wasreached.Amongthefamiliesofdetectedmetabolites,large

Ngày tải lên: 25/12/2022, 00:49

... ceramics) — Determination of coating thickness by crater-grinding method 1 Scope This International Standard specifies a method for the determination of the thickness of ceramic coatings by a crater-grinding ... competence of testing and calibration laboratories 3 Symbols For the purpose of this document, the following symbols apply D best estimate of the outer diameter of the crater, at the surface of the ... (see Figure 1); d best estimate of the inner diameter of the crater, defined by the bottom of the coating layer, in micrometres (see Figure 1); h thickness of the coating, in micrometres (see

Ngày tải lên: 09/03/2024, 15:34

... (AEW) of 59 g, jeffamine (Aldrich) with an AEW of 100 g and 4,40-diaminodiphenylsulfone (Aldrich, 97%) with an AEW of 62 g BPA and naphtalene were supplied by Aldrich Water was purified by means of ... The wt% of BPA in the epoxy/amine mixture is calculated by comparing the integral of the four aromatic protons of BPA at 6.98 ppm (Ia) with the integral of the four aromatic protons of naphthalene ... 1C decrease of the Tg of epoxy/DDS/BPA (1/1/ 0.076) compared to the Tg of epoxy/DDS (1/1) was noted (respective Tg onset of 164 and 174 1C) Owing to the low amount of free BPA (o10% of the initial

Ngày tải lên: 17/12/2017, 14:32

... sample matrices [18, 32–35] This approach makes use of the addition of known amounts of analytes of interest to multiple aliquots of the sample and of another non-spiked, baseline aliquot, i.e., the ... employed for the mass spectrometry anal-ysis of the analytes to identify and measure the level of ions as summarized in Table 1 A series of amounts (0, 20, 40, 60, and 120 μL) of standard solution ... for the determination of volatile compounds in jujube extract by gas chromatography/mass spectrometry Anal Lett 47:654–674 6 Zhao J, Li SP, Yang FQ, Li P, Wang YT (2006) Simultaneous determination

Ngày tải lên: 29/05/2020, 12:34

... method for determination of 16 FDA polycyclic aromatic hydrocarbons (PAHs) in mainstream cigarette smoke by gas chromatography–mass spectrometry Abstract A gas chromatography–mass spectrometry ... chromatography–mass spectrometry, High resolution mass spectrometry, Low resolution mass spectrometry, Accelerated solvent extraction © The Author(s) 2018 This article is distributed under the terms of ... fortified with 40  ng of native standards, 100  ng of internal standards and extracted following the analytical procedure quality control samples were calculated by division of the mass of PAHs quantified

Ngày tải lên: 29/05/2020, 12:54

... voltage of 3500 V, cone voltage of 45 V, source block temperature of 120 oC, cone gas of 60 L h–1, desolvation temperature of 350 oC, desolvation gas of 500 L h–1, collision gas flow rate of 0.2 ... pesticides in tea-tree plantation soil by ultra-performance liquid chromatography tandem mass spectrometry Ho Chi Minh City University of Education Journal of Science, 18(3), 403-413 Trang 2Hashimoto, ... effects by changing the hydration volumes from 5 mL to 10 mL and also by the variation in the centrifuge velocity of 4000, 5000, and 10000 rpm The matrix effects were evaluated by the calculation of

Ngày tải lên: 28/06/2021, 10:09

... h e m / Research Article Development of a sample preparation strategy for the determination of tungsten in soil samples by inductively coupled plasma mass spectrometry using a response surface ... the form of tungstate in soils, with the majority of forms being 9, respectively, leading to the possibility of soluble complex formation with a variety of inorganic and organic effects of its compounds ... sensitivity Thus, the purpose of this work was to evaluate and improve the sample preparation strategy for the determination of W from soil samples of abandoned W mining areas by developing an efficient,

Ngày tải lên: 13/01/2022, 00:23

... 1Simultaneous determination of degradation products ofnonylphenol polyethoxylates and their halogenated derivatives by solid-phase extraction and gas chromatography–tandem mass spectrometry after ... N,O-bis(trimethysilyl)acetamide, all the analytes were determined by gas chromatography–tandem mass spectrometry (GC–MS–MS) with electron ionization (EI) The ion peaks of [M − 85]+ of the derivatives were selected as precursor ... 255 271 299 Cl OTMS m/z = 241 + Fig 1 EI–mass and EI–tandem mass spectra of ClNP1EO and ClNP1EC. as the precursor ions Fig 1 shows the mass spec-tra of EI–MS and EI–MS–MS under the optimum CID

Ngày tải lên: 18/10/2022, 15:41

... efficiency of 2HG in mass spectrometry Chalmers et al.19 used chiral derivatization reagent of (D)-2-butanol and acetic anhydride to derivatize 2HG followed by gas chromatography/mass spectrometry ... concentration of DATAN was high (250 mM), which may cause contamination of mass spectrometer Therefore, derivatization of 2HG by TSPC offers much better detection sensitivities than by DATAN In ... Trang 1Sensitive Determination of Onco-metabolites of D- and L-2-hydroxyglutarate Enantiomers by Chiral Derivatization Combined with Liquid Chromatography/Mass Spectrometry Analysis Qing-Yun

Ngày tải lên: 04/12/2022, 16:24

... the determination of multi-class drugs of abuse in human hair by LC-ESI-MS/MS For this pur-pose, we selected hexanol-based SUPRAS [25] , which consists of inverted hexagonal aggregates of the ... his-tory of drugs of abuse Sampling was carried out under the data protection and management of biological samples policy estab-lished by the ethical committee of the University of Córdoba ... Samples were fortified with 8 pg mg−1 of drugs of abuse and 40 pg mg−1 of IS by adding the proper volume ( < 20μL) of working solutions containing a mixture of analytes in acetonitrile The sample

Ngày tải lên: 20/12/2022, 21:24

... Trang 1Simultaneous determination of borax and polyphosphates content in food by liquid chromatography inductively coupled plasma mass spectrometry (LC-ICP-MS) Dinh Viet Chien ... analyzed by LC-ICP-MS system The method was validated with calibration curves that have the coefficient of determination (R2) higher than 0.995 The method limits of detection (LOD) of borax, ... coefficient of determination (R2) was higher than 0.996, the coefficient of variation was less than 4.5%, and the recovery was in the range of 87.2 to 101.1% In addition, a method of capillary

Ngày tải lên: 26/04/2024, 21:06

... of Chemistry, University of Joensuu, Finland 2 Biophysics Division, Petersburg Nuclear Physics Institute, Gatchina, Russia 3 Department of Chemistry, Mass Spectrometry Institute, University of ... charge-normalized by dividing the intensity of each signal by the respective z This should reduce any bias introduced by a possible shift of the charge state distribution caused by ligand binding ... requirement of three The crystal structures of CTX– inhibitor complexes gave interesting insights into the binding Surprisingly, none of the inhibitors occupied any of the aglycone subsites of the

Ngày tải lên: 07/03/2014, 17:20

... (A) MALDI-TOF-TOF mass spectrum of the light (d0) and heavy (d4) precursor ions presenting a mass difference of 4.024 Da (indicated by the curved arrow) (B) Fragmentation mass spectrum of the double-charged ... modified by nondeuterated and deuterated cross-linkers by MALDI-TOF-TOF mass spectrometry A selection of mass spectra illustrates how the comparison of chymotryptic peptides from different cross-linked ... were detected by MS using the 4.0247 atomic mass unit (amu) mass difference conferred by the binding of the nondeuterated or deuterated cross-linkers (Fig 3A) [13,29] Detection of modified peptides...

Ngày tải lên: 15/03/2014, 00:20

... semiquantitive assessment of the relative levels of toxicity of the different mutants was derived by comparing the number of colonies and growth of the spots at the different levels of dilution 4984 CD ... nucleotide is formed by hydrolysis of the 2¢ : 3¢-cyclophosphate group Additional interactions stabilize the initial intermediate of the reaction Following determination of the structure of the complex ... vivo inhibition of Analysis of Kid RNase model cell growth, underlining their relevance to our understanding of the basic activities of this toxin Results Selection and isolation of Kid mutants...

Ngày tải lên: 16/03/2014, 03:20

... enzymatic digestion of proteins to peptides, followed by chromatographic separation of the peptides and electrospray ionization directly into the source of a mass spectrometer The mass spectrometer ... then signals of low abundance will not be selected or 'picked' for sequencing by the instrument The overall protein coverage of the experiment is a function of the sensitivity of the mass spectrometer, ... to the use of more modern mass spectrometric equipment, we were able to identify many more proteins than previous largescale studies Virtually all of the proteins discovered by mass spectrometry...

Ngày tải lên: 14/08/2014, 16:21