detection identification and quantitation of haemglobinopathies

... genomic clones of observed. Trang 5Table 2 Summary of patient tissues and fluids used for the detection of HPVTrang 6-Table 2 Summary of patient tissues and fluids used for the detection of HPV (Continued)Trang ... region of known HPVs and should allow for detec-tion of any of these types The second qPCR assay tar-gets the coding region of the hypervariable loop V of the L1 gene of HPV6, 11, 16 and 18 These ... from smoker drinkers and 22 from non smoker non drinkers (Table 2) Design of specific primers and probes for multiplex detection of HPV types 6, 11, 16 and 18 by qPCR To amplify and detect the most...

Ngày tải lên: 12/08/2014, 04:20

... allele and wild-type allele For detection of theBRAF mutation, the selectivity was 0.5% of the mutant allele, and for the identification ofHRAS and NRAS mutations, it was 2% The detection of theRET-PTC1 ... adenomas and b) groups FVPTC and FTC Conse-quently, the molecular marker profile of some FVPTC samples was similar to that of classic PTC, whereas this profile of some others resembled that of FTC ... indicator of Hürthle cells in a clinical sample because, according to the literature [26], the mtDNA content of these cells is significantly higher MiRNA detection and quantitation The detection of...

Ngày tải lên: 17/06/2020, 18:18

... main isoflavonoids, formononetin, ononin and calycosin was validated Results and Discussion HPLC-DAD analysis of flavonoid standards The purpose of this assay was to obtain chromatograms and LC/ESI-MS ... relative standard deviations of retention times of ononin, calycosin and Trang 7formononetin were 0.24, 0.18 and 0.13, respectively Six replicate analyses of quality control samples and a spiked ... with DAD and ESI-MS method for the identification of isoflavonoids in Astragali Radix was developed As examples, eight isoflavonoids were identified in A mongholicus and A membranaceus, two of the...

Ngày tải lên: 02/11/2022, 11:34

... infections and/or subsequent virus recombination events have occurred [1] Therefore, the development of tools for the detection of viruses, the characterization of their genomes and the study of their ... along with the general application of this technology for the identification of viral species and their genes in other virus families Finally, the software and Web site that we have developed ... sensitivity of the PCR amplification Whereas highly degenerate primers consisting of pools of hundreds or thousands ofprimers with different DNA sequences may allow amplifi-cation of DNA templates...

Ngày tải lên: 18/06/2014, 22:20

... peak of the correct T m in dissociation or melting curve analysis plots. The accurate estimation of the quantity of HSV was expressed as viral copies/ml of CVL. The lower limit of quantitation of ... cycles. Standards of 10 6 to 10 copies per reac- tion of C. trachomatis pgp3 amplicons and negative con- trols were included in each PCR reaction to generate a standard curve and quantities of the ... oxidase and carbohydrate oxidation as confirmation of N. gonorrhoea. Candida, Trichomonas vaginalis and clue cells A few drops of saline was used to make a wet preparation of the high vaginal swab and...

Ngày tải lên: 19/06/2014, 08:20

... standardization and testing were carried out on sepa-rate sets of images, two for training and 12 for testing The number of samples used for standardization was 4, 7 and 10 for the A, B, and C test ... Characterization of the structural morphology of virus particles in electron micrographs is a complex task, but desirable in connection with investigation of the maturation process and detection of changes ... micrographs and is then employed to classify and quantify similar structures of interest in an unlimited number of electron micrographs by a process of correlation Results: Practical application of the...

Ngày tải lên: 20/06/2014, 01:20

... its growth and development, and standard methods for detection and treatment Special emphasis is focused on interactions between ovarian tumors and the innate and adaptive immune system and attempts ... treatment of ovarian cancer has come from intraperitoneal admin-istration of platinum and taxane agents instead of the more conventional intravenous delivery of these drugs [71-73] Of the 654 randomized ... USA.2Department of Obstetrics and Gynecology, University of Wisconsin-Madison, 600 Highland Ave, Madison, WI, 53792, USA. Authors ’ contributions JAAG, JPC, and MSP did the majority of the writing of this...

Ngày tải lên: 20/06/2014, 07:20

... EDTA, 1.5 M NaCl), in the presence of 0.1 mg of proteinase K and 1 mg of lysozyme and incubated at 37°C for 30 min Lysis was completed by adding 1 ml of 20% SDS and incubated for 2 h at 65°C with ... between 45°C and 55°C with half-life of 60 and 20 min respectively (Figure 7) Effects of metal ions and additives TheAS-protease activity was estimated in the presence of metal ions (5 mM) and different ... col-lected and solubilised in 3 ml of cold 2 M urea contain-ing 20 mM Tris-HCl buffer, 0.5 M NaCl and 2% Triton X-100 (pH 8.0) and centrifuged at 10,000 × g for 10 min The supernatant was discarded and...

Ngày tải lên: 21/06/2014, 05:20

... Figure 10: Samples of indoor and outdoor test video clips for counting the face detection rates and false positives Table 1: Face detection and false positive rates of the indoor and outdoor test ... videos of different subjects are collected to train, test, and compare the proposed streaming face recognition algorithms 4.1 Face detection and tracking Evaluation of the face detection and tracking ... accuracy, and robustness of the view generation and recog-nition modules Finally, the results of multiple levels of visual context analysis need to be integrated to develop an awareness of the human...

Ngày tải lên: 22/06/2014, 19:20

... ‘chemical genetic’ approach of identifying and studying the mechanism of action of small-molecule ago-nists (and antagoago-nists), we hoped to uncover some of the complexities of the Hh-signaling system ... concentrations of Hh-Ag 1.3 (n = 6 explants) Error bars represent standard deviations (c-n) Confocal images of representative explants cultured in the presence of different concentrations of the agonist and ... exhibited overgrowth of the headfolds and hindbrain, reminiscent of to show fused ventral lips of the cephalic folds, and a single continuous optic vesicle, indicating lack of a clearly defined...

Ngày tải lên: 06/08/2014, 18:20

... Trang 1R E S E A R C H Open AccessIdentification and analysis of unitary pseudogenes: historic and contemporary gene losses in humans and other primates Zhengdong D Zhang1, Adam Frankish2, ... pheromone receptors (V2Rs) of the accessory olfactory neural pathway and, compared to other mammals being examined, there is a co-expansion of MUPs and V2Rs in mouse, rat, and opossum [24] Our analysis ... pseudogenes from the tandem gene families are indicated by gray bars Inset: box plot of the number of human unitary pseudogenes in each paralogous gene set and the number of members in each paralogous...

Ngày tải lên: 09/08/2014, 20:21

... tests, the field would benefit from additionalsoftware tools and methodologies for strengthening ourunderstanding of the distribution and variation ofhomozygous segments/contiguous homozygosity ... the four HapMap population samples and showed that average total length of homozygosity was highest and almost equal between JPT and CHB, lowest in YRI, and of an intermediate level in CEU (mean ... Analysis of multi-hit hzAnalyzer segments indicated that PLINK had split a number of runs with over several thousand loci into smaller ROHs A likely cause of this discrepancy were random groups of...

Ngày tải lên: 09/08/2014, 22:23

... prediction, annotation and validation of the expressed genes in common bean The availability of large sets of annotated sequences as derived by identification, sequencing, and validation of genes expressed ... help in the development of an accurate and complete structural annotation of the common bean genome, a valid tran-scriptome map, and the identification of the genetic basis of agriculturally important ... also help in the iden-tification of transcription factors and small RNAs in common bean, understanding of gene families, and very importantly the development of molecular markers for common bean...

Ngày tải lên: 11/08/2014, 11:21

... amino-acid sequence of CaNDR1a (Figure 4), with resi-dues S189 and G190 being strongω-site candidates (with P-values of 2.48 × 10-6and 2.76 × 10-5, respectively) Furthermore, CaNDR1a and its Arabidopsis ... C-terminal end of CaNDR1a is removed from the mature protein in tobacco (a) Constructs used for transiently expressing HA- and HA-His-tagged CaNDR1a proteins in tobacco leaves (b) Detection of CaNDR1a-tagged ... molecular weight of 23.8 kDa and an isoelectric point of 9.58 Searching for Arabidopsis relatives of our proteins, we screened the GenBank database by means of the BLAST P algorithm [26] and retrieved...

Ngày tải lên: 11/08/2014, 11:21

... Lhcb1 isoforms of several plants, and Thr39, which is not conserved across Lhcb1 isoforms of other plants, but is present as a non-phosphorylated residue in the Lhcb1 isoforms of Arabidopsis and ... patterns: 85% pSer and 10.6% pThr [22] and 88% pSer and 11% pThr [33] in Arabidop-sis; and 86% pSer and 12.7% pThr in M truncatula [49] Only 1.2% (2) of the phosphorylation events of these phos-phopeptides ... phosphoproteomic analysis of woody plants Results Identification and characterization of the phosphoproteome of DTBs Total proteins were isolated from DTBs of poplar, and then digested with trypsin...

Ngày tải lên: 11/08/2014, 11:21

... nutritional, nutraceutical and health benefits [3,4]. With increased awareness in many societies of the escalating incidence of obesity and the associated risk of diabetes and cardiovascular disease, ... theoretical molecular mass and pI of. .. expression of the allergenic soybean seed P34 protein [42] The lack of conservation of allergenic epitopes between species, and the fact that many ... and two-dimensional electrophoretic identification of IgE-binding polypeptides of Lupinus albus and other legume seeds Journal of Agricultural and Food Chemistry 2005, 53(11):4567-4571...

Ngày tải lên: 11/08/2014, 11:21

... However, identification of long npcRNAs is limited to a few plant species, such as Arabidopsis, rice and maize, no systematic identification of long npcRNAs and their responses to abiotic and biotic ... for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization (MOE) and Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, ... is one of the most devastating diseases of wheat in China and worldwide and causing significant yield losses [28] High temperature, often combined with drought stress, causes yield loss and reduces...

Ngày tải lên: 11/08/2014, 11:21

... the knowledge of the genetic regulation of growth and development, flowering and sex-determi-nation is very scarce, yet essential to accelerate breeding and aid our understanding of flowering ... required for sepal and petal identity On the other hand, the role of euAP1 genes in specification of sepal and petal identity in plants other than Arabidopsis is unclear and the concept of the A function ... FUL and SEP4; weaker interactions were detected between AG and SEP4 and SEP3 and SEP4 No interactions were identified with FUL-like and SEP1 (Figure 2H) Expression patterns in vegetative and...

Ngày tải lên: 11/08/2014, 11:22

... phosphorylation pattern of H3S28 is similar to that of H3S10ph during mitosis [4,5]. Because the spatial and temporal patterns of H3S10ph and H3S28ph are consistent with chro mo- some condensation and decondensation, ... them (Arg2, Lys4, Ser10, and Gly12). Using anti-H3T3ph antibodies, bands were detected in the case of normal histone H3 and S10A, T11A, and G12A mutants, but not in the case of R2A, T3A, or K4A mutants ... Page 7 of 14 was expressed in meristems and primordia of root tips, lateral roots (Figures 5C and 5D), the shoot apex, leaf (Figures 5E-5H), and flowers (Figures 5I-5K). Fluores- cent signals of...

Ngày tải lên: 11/08/2014, 11:22