desorption ionization time of flight mass spectrometry maldi tof
evaluation of comprehensive 2d gas chromatography time of flight mass spectrometry for 209 chlorinated biphenyl congeners in two chromatographic runs
... spectra/s) of a time-of-flight mass spectrometer (TOFMS) for the identification of PCB congeners in minimal analysis time Taking advantage of the full mass range data acquisition of the TOFMS allowed ... two-dimensional gas chromatography 2D GC coupled with a time-of-flight (TOF) mass spectrometer for the potential separation of 209 PCB congeners, using a sequence of 1D and 2D chromatographic modes In two ... use of fast gas chromatography coupled with time-of-flight mass spectrometry for the analysis of PCB congeners [13, 14] Their work demonstrated the use of narrow bore capillary columns and the fast...
Ngày tải lên: 02/11/2022, 09:28
Tissue-specific metabolite profiling and quantitative analysis of ginsenosides in Panax quinquefolium using laser microdissection and liquid chromatography– quadrupole/time of flight-mass
... ARTICLETissue-specific metabolite profiling and quantitative analysis of ginsenosides in Panax quinquefolium using laser microdissection and liquid chromatography– quadrupole/time of flight-mass spectrometry Yujie ... quadrupole/time-of-flight-mass spectrometry (UHPLC-Q/TOF–MS) to elucidate the distribution pattern of ginsenosides in tissues The contents of ginsenosides in various tissues were also compared Results: A total of 34 ... the combination of fluorescence microscopy, laser micro-dissection (LMD), and ultra-high performance liquid chromatography-quadrupole/time-of-flight-mass spec-trometry (UHPLC-Q/TOF–MS) has been...
Ngày tải lên: 29/05/2020, 13:39
A comprehensive study of pomegranate flowers polyphenols and metabolites in rat biological samples by high-performance liquid chromatography quadrupole time-of-flight mass
... high-performance liquid chromatography quadrupole time-of- flight mass spectrometry (HPLC-Q-TOF-MS 2) method, we charac- terized the phytochemical profile of pomegranate flowers extract Furthermore, the ... Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China b University of Chinese Academy of ... investigation conditions The HPLC-Q-TOF-MS 2 system (Agilent Technologies, Palo Alto, CA, USA) which consisted of a HPLC system (1260 Series, cou- pled to an Agilent Q-TOF mass spectrometer equipped with...
Ngày tải lên: 25/12/2022, 00:45
Ionization Methods in Organic Mass Spectrometry pot
... Trang 1Ionization Methods in Organic Mass Spectrometry Contents: Introduction Gas-Phase Ionization Electron Ionization (EI) Chemical Ionization (CI) Desorption Chemical Ionization (DCI) ... Negative-ion chemical ionization Field Desorption and Ionization Field Desorption (FD) Field Ionization (FI) Particle Bombardment Fast Atom Bombardment (FAB) Secondary Ion Mass Spectrometry (SIMS) ... Atmospheric Pressure Ionization Electrospray Ionization (ESI) Atmospheric Pressure Chemical Ionization (APCI) Laser Desorption Matrix-Assisted Laser Desorption Ionization (MALDI) Appendices...
Ngày tải lên: 22/03/2014, 14:20
báo cáo khoa học: " Analysis of expressed sequence tags from a single wheat cultivar facilitates interpretation of tandem mass spectrometry data and discrimination of gamma gliadin proteins that may play different functional roles in flour" ppt
... I C L E Open AccessAnalysis of expressed sequence tags from a single wheat cultivar facilitates interpretation of tandem mass spectrometry data and discrimination of gamma gliadin proteins that ... portion of the 3’ end of coding region. 5 contig is missing a portion of the 5 ’ end of the coding region. Trang 4V are very similar in the different Butte 86 proteins andthe positions of 6 cysteine ... predicted MWs of the mature pro-teins range from 30,361 to 38,899 and the predicted pIs of the proteins range from 7.5 to 8.1 (Table 2) Figure 1 shows a comparison of the amino acid sequences of the...
Ngày tải lên: 12/08/2014, 03:21
Ionization methods in organic mass spectrometry
... laser desorption ionization’* (MALDI; see Chapter 7) in the field of biochemistry, and MALDI is ideally suited to TOF analysis In a TOF analyser, a ‘pulse’ or ‘bundle’ of ions is accelerated out of ... the flight time of the ion, v = the velocity of the ion, and 1 = the length of the flight By eliminating v from these two equations, the mass of an ion can be calculated if the time of flight, ... where m = the mass of an ion, e = the charge on an electron, V = the accelerating voltage, t = the flight time of the ion, and 1 = the length of the flight The mass range of a TOF analyser is...
Ngày tải lên: 18/05/2022, 09:28
impact of point spread function modelling and time of flight on fdg uptake measurements in lung lesions using alternative filtering strategies
... modelling [20,21] and time of flight (TOF) [22] have become commercially available on PET/CT scanners, with TOF also available on PET/MR [23] The use of PSF modelling, with and without TOF, has been shown ... (OSEM) re-construction; OSEM with TOF (TOF); OSEM with PSF modelling - Siemens HD·PET (HD); and OSEM with both PSF and TOF - Siemens ultraHD·PET (UHD) For non-TOF reconstructions, 3 iterations ... subsets (3i24s) were used, while for non-TOF re-constructions, 2 iterations and 21 subsets (2i21s) were used Two iterations were chosen for TOF reconstructions as TOF has been shown to provide faster...
Ngày tải lên: 02/11/2022, 11:37
from cow to cheese genetic parameters of the flavour fingerprint of cheese investigated by direct injection mass spectrometry ptr tof ms
... spectrometry method (proton transfer reaction-time of flight-mass spectrometry, PTR-ToF-MS) was used for the first time to obtain the fingerprints of volatile compounds in the same model cheeses ... traits in cheese The aim of this work was to infer genetic parameters of the profile of volatile compounds in cheese as revealed by direct-injection mass spectrometry of the headspace gas from ... RESEARCH ARTICLE From cow to cheese: genetic parameters of the flavour fingerprint of cheese investigated by direct‑injection mass spectrometry (PTR‑ToF‑MS) Matteo Bergamaschi1, Alessio Cecchinato1*,...
Ngày tải lên: 04/12/2022, 10:33
Identification of plant polysaccharides by MALDI-TOF MS fingerprinting after periodate oxidation and thermal hydrolysis
... the MALDI-TOF mass spectrum 3.5 Exploratory PCA and HCA analysis of MALDI-TOF mass spectra of thermally treated pOx-PS samples To illustrate and emphasize sources of variations among MALDI-TOF ... chromatography; MALDI-TOF MS, Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry; MLG, barley mixed-linked β-glucan; MS, mass spectrometry; Mw, molecular weight; m/z, mass-to-charge ... use of for example liquid chro-matography coupled to mass spectrometry (LC-MS) (Remoroza et al., 2014) and Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF...
Ngày tải lên: 01/01/2023, 14:03
Study on the analytical application of matrix assisted laser desorption ionization mass spectrometry imaging technique for visualization of polyphenols
... application of matrix-assisted laser desorption/ionization mass spectrometry-imaging techniquefor visualization of polyphenols Nguyen Huu Nghi Kyushu University 2018 Trang 2List of contentsChapter ... 3.1 Screening of matrix reagents for negative MALDI-MS detection of monomeric and condensed catechins 16 3.2 Effect of concentration of nifedipine on negative MALDI-MS detection of monomeric and ... source of the present MALDI-MS system, thewavelength of MALDI laser (355 nm) was adequate for the excitation ofnifedipine (maximal UV-wavelength of 344 nm [54]), causing possible formationof a...
Ngày tải lên: 16/11/2018, 04:00
Study on the analytical application of matrix assisted laser desorption ionization mass spectrometry imaging technique for visualization of polyphenols
... application of matrix-assisted laser desorption/ionization mass spectrometry-imaging technique for visualization of polyphenols Nguyen Huu Nghi Kyushu University 2018 Trang 2List of contents ... 3.1 Screening of matrix reagents for negative MALDI-MS detection of monomeric and condensed catechins 16 3.2 Effect of concentration of nifedipine on negative MALDI-MS detection of monomeric ... source of the present MALDI-MS system, the wavelength of MALDI laser (355 nm) was adequate for the excitation of nifedipine (maximal UV-wavelength of 344 nm [54]), causing possible formation of a...
Ngày tải lên: 28/03/2021, 20:56
Study on the analytical application of matrix assisted laser desorption ionization mass spectrometry imaging technique for visualization of polyphenols
... Identification of catechin oligomers from apple (Malus pumila cv Fuji) in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and fast-atom bombardment mass spectrometry ... Chien Characterization of grape procyanidins using highperformance liquid chromatography/mass spectrometry and matrix-assisted laser 83 desorption/ionization time-of-flight mass spectrometry J Agric ... extract by MALDI-TOF mass spectrometry J Agric Food Chem 2004, 52, 2455 [35] M Monagas, J E Quintanilla-López, C Gómez-Cordovés, B Bartolomé, R Lebrón-Aguilar MALDI-TOF MS analysis of plant proanthocyanidins...
Ngày tải lên: 29/03/2021, 07:15
Study on the analytical application of matrix assisted laser desorption ionization mass spectrometry imaging technique for visualization of polyphenols
... Identification of catechin oligomers from apple (Malus pumila cv Fuji) in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and fast-atom bombardment mass spectrometry ... Chien Characterization of grape procyanidins using highperformance liquid chromatography/mass spectrometry and matrix-assisted laser 83 desorption/ionization time-of-flight mass spectrometry J Agric ... extract by MALDI-TOF mass spectrometry J Agric Food Chem 2004, 52, 2455 [35] M Monagas, J E Quintanilla-López, C Gómez-Cordovés, B Bartolomé, R Lebrón-Aguilar MALDI-TOF MS analysis of plant proanthocyanidins...
Ngày tải lên: 30/03/2021, 14:23
Study on the analytical application of matrix assisted laser desorption ionization mass spectrometry imaging technique for visualization of polyphenols
... in Mass Spectrometry in 1997, detailing the identification of these compounds through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and fast-atom bombardment mass ... from black tea extract by MALDI-TOF mass spectrometry J [35] M Monagas, J E Quintanilla-López, C Gómez-Cordovés, B Bartolomé, R Lebrón-Aguilar MALDI-TOF MS analysis of plant proanthocyanidins ... procyanidins using high- performance liquid chromatography/mass spectrometry and matrix-assisted laser 84 desorption/ionization time-of-flight mass spectrometry J Agric Food Chem [53] K Suyama, M Shirai...
Ngày tải lên: 19/06/2021, 09:21
Báo cáo khoa học: Determination of thioxylo-oligosaccharide binding to family 11 xylanases using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and X-ray crystallography pot
... of Chemistry, University of Joensuu, Finland 2 Biophysics Division, Petersburg Nuclear Physics Institute, Gatchina, Russia 3 Department of Chemistry, Mass Spectrometry Institute, University of ... weight of softwoods, 15–30% of hardwoods and up to 30% of annual graminaceous plants [7,8] Endo-1,4-b-xylanases (EC 3.2.1.8) are O-glycoside hydrolases that catalyze a random hydrolysis of inter-nal ... [65,66] On the basis of the mass spectra presented in Fig 3, all proteins had a variable degree of modifica-tion The first peaks at each charge state in the mass spectrum of TRX I (Fig 3A) represent...
Ngày tải lên: 07/03/2014, 17:20
Real-time Monitoring technology of Mass spectrometry Speaker
... Real-time Monitoring technology of Mass spectrometry Speaker : Achong Outline IMS PTR-MS SIFT-MS Application of IMS IMS-TOF IMS-IMS-TOF Application of PTR-MS PIT-MS Real-time Monitoring ... 191-241 J Mass Spectrom Ion Processes, 173 (1998) 191-241 J Mass Spectrom Ion Processes, 173 (1998) 191-241 PTR mass spectrometry Advantages: Soft ionization and low matrix effect Real-time ... limit of detection (0.5 ppbv for 1-min measurements) Disadvantages: A large number of VOCs could not be identified and... Reviews, 2005, 24, 661– 700 Application of IMS IMS/TOF IMS/IMS/TOF...
Ngày tải lên: 28/04/2014, 13:18
16S ARDRA and MALDI TOF mass spectrometry as tools for identification of lactobacillus bacteria isolated from poultry
... objective of our study is to evaluate the potential use of Amplified 16S Ribosomal DNA Restriction Analysis (16S-ARDRA) and MALDI-TOF mass spectrometry (MS) as methods for species identification of ... and L ingluviei Conclusions: The MALDI-TOF MS and 16S-ARDRA assays are valuable tools for the identification of avian lactobacilli to the species level MALDI-TOF MS is a fast, simple and cost-effective ... discriminatory power of ARDRA depends on the correct choice of restriction endonucleases Species identification of poultry lactobacilli determined on the basis of 16S-ARDRA was confirmed by MALDITOF MS and...
Ngày tải lên: 19/11/2022, 11:35
Tài liệu Báo cáo khoa học: Comparison of membrane fraction proteomic profiles of normal and cancerous human colorectal tissues with gel-assisted digestion and iTRAQ labeling mass spectrometry pptx
... quantitation, we used multi-q [41] software to analyze the iTRAQ data Raw data files from the Waters Q-Tof premier mass spectrometer were converted into files of mzXML format using masswolf (Institute for ... roles in the pathogenesis of CRC In general, factors that contribute to the pathogenesis of CRC include the accumulation of mutations and the deregulation of gene expression Of particular interest ... software (Fuji PhotoFilm, Tokyo, Japan) Data were analyzed by an unpaired t-test using the statistical software spss ⁄ windows 11.0 statistical package (SPSS Inc, Chicago, IL, USA) P values of...
Ngày tải lên: 16/02/2014, 15:20
Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc
... One of the early demonstrations of the role of carbohydrates in recognition was binding of the influenza virus to red blood cells via sialic acid [1], and later by work on the chemical basis of ... and CID MS/MS were carried out on a Micromass (Manchester, UK) Q-Tof mass spectrometer Nitrogen was used as desolvation and nebuliser gas at a flow rate of 250 LỈh)1 and 15 LỈh)1, respectively ... and sequence analysis of underivatized oligosaccharides by combined MS/MS of singly and doubly charged molecular ions in negative-ion electrospray mass spectrometry J Am Soc Mass Spectrom 13, 670–679...
Ngày tải lên: 19/02/2014, 12:20
Báo cáo khoa học: Systematic quantification of complex metabolic flux networks using stable isotopes and mass spectrometry pptx
... (Eur J Biochem 270) Heizle (2001) [14] used MALDI-TOF-MS to measure the mass isotopomer distribution of extracellular metabolites for the determination of the Corynebacterium glutamicum metabolic ... quantification of their activity in vivo requires the use of isotopic tracers [5,35,38] The extent to which the use of MS measurements of biomass hydrolysates after the introduction of the 13C tracer ... 2003) labeled substrate, the determination of the range of most of the exchange fluxes and the validation of the flux estimates through satisfaction of redundancies Specifically, we determined that...
Ngày tải lên: 08/03/2014, 08:20