describe the effect of dna binding domain

... stage The association of subdomains results in the formation of the closed b barrel burying the hydrophobic core At present there is no experi-mental evidence for the occurrence of the domain-swapped ... required Because the domain-swapped dimer of Bc-Csp has only been observed in the presence of a bound dT6 strand, the question remains whether DNA binding to the CSP is responsible for domain swapping ... crystal structures The extended irregular conformation of dT6 oligonucleotides in the binding site, the unstacking of bases with respect to each other upon binding, and the shielding of the nu-cleobase

Ngày tải lên: 23/03/2014, 09:21

... effect of dsDNA on the reactivity of SmD1-negative samples (amino acids 83–119) Increasing concentrations of dsDNA (recombinant plasmid) showed an inhibitory effect on the binding of anti-dsDNA ... in the detection of anti-dsDNA antibodies in the SmD peptide ELISA [32] In the present study we confirmed the dsDNA binding property of the SmD1 peptide This finding was further supported by the ... ELISA for the detection of anti-Sm antibodies Depending on the cut-off value of the SmD1 ELISA, we found a high degree of concordance between the two tests At an optimized cut-off value of 100 units

Ngày tải lên: 09/08/2014, 10:20

... outlined in method 1.RESULTSCompare the tiller number of the rice plantIn the analysis of chemical soil, the data presented in Fig 3.1 shows that the average number of tillers per pot increased for ... presence of other contaminants All samples demonstrated satisfactory DNA quantity, exceeding 100 ng/µL; however, the DNA quality was found to be below the acceptable threshold of 1.7. Table 3.8 DNA ... quality of the extracted DNA were assessed using a nanodrop spectrophotometer, where the A260/280 ratio indicates protein contamination and the A260/230 ratio reveals the presence of other contaminants.All

Ngày tải lên: 21/04/2022, 15:48

... the DNA quality was found to be lower than the desired threshold of 1.7, as indicated by the A260/280 and A260/230 ratios, which are indicative of protein contamination and the presence of other ... ethanol Furthermore, distilled water was swapped with nuclease-free water (NEB, UK) to enhance the purity of the process.RESULTSCompare the tiller number of the rice plantFigure 3.1 Averages of tiller ... protein contamination and the A260/230 ratio signifying the presence of other contaminants.All of the samples have good DNA quantity (more than 200 ng/àL) However, DNA quality is lower than 1.5

Ngày tải lên: 30/12/2023, 03:21

... difficult the interpretation of theresults of econometric analysis inter-The fact that people able to attain a higher level of schools have other competences thatmakes them earn more during their ... master thesis I will firstly revise the theory on returns to schooling, either private or social By doing this, I will present the Mincerian regression, one of the most calculatedequations in the ... examining the ef-fect of compulsory schooling in the UK Some other studies have taken other IV strategiesusing natural experiments and most of them conclude that IV estimates exceed their corre-sponding

Ngày tải lên: 24/08/2013, 18:40

... and the fuel cell relies on the ambient relative humidity and water production in the cathode for the humidification of the membrane; (x.) the circulating ambient air facilitates the cooling of ... detrimental effect of the fuel cell performance 2.1 Computational domain The full computational domains for the planar air-breathing PEM fuel cell consists of anode gas flow field and the membrane ... g is gases thermal conductivity (W/(m.K)) The gas phase and the liquid phase are assumed to be in thermodynamic equilibrium; hence the temperature of the liquid water is the same as the gas phase

Ngày tải lên: 05/09/2013, 14:58

... EGT Since, the result of Figure 7 that the EGT of DF was higher than that of JBD blends Therefore, the NOX emissions of JBD blends were lower than that of DF At 0% EGR, the NOX emission of JB5 and ... across the orifice plate, to measure the pressure difference inside the inlet pipe and the atmosphere The coefficient of discharge of the orifice is determined experimentally to be 0.603 Simple software ... of renewable energy; biofuel is one of them, but it is the most important one [1] Biofuel oils can Trang 2particularly interesting, as these are generally cheaper than edible oils Moreover, the

Ngày tải lên: 05/09/2013, 16:11

... Trang 1Đo kiểm đánh giá can nhiễu mạng truyền hình cáp The Measurement and Analysis of the effect of interference in Television Cable network NXB H : ĐHCN, 2012 Số trang 101 ... communications – The Morgan Kaufmann Serries in Networking Editor – 2nd Edition 17 ROHDE & SCHWARZ - SOUND and TV BROADCASTING – CCIR and FFC tv standards – Printed in the federal republic of Germany ... structure, channel coding and modulation systems for Broadcasting, Interactive Services, News Gathering and other broadband satellite applications (DVB – S2) 14 Tim Williams – EMC for Product Designers

Ngày tải lên: 26/11/2013, 20:44

... they had the material and spiritual support from their country, they were hopeful of winning the war But in the soldiers’ return, the flowers given to them on their graves become the symbol of ... stop them loving each other as nothing can stop the rose’s life Here the rose is the basis of the idea of everlasting love. Tyger Tyger, burning bright,“literary texts are In the forests of the ... givenflowers by their women These flowers carry the important symbols in them In the soldiers’ departure, the flowers are the symbol of hope in victory, because when they departed, their forces

Ngày tải lên: 22/12/2013, 12:59

... i , the distribution of the counterfactual outcome C i(0) in the treated group is the same as the (observed) distribution of C i(0) in the non-treated group In other words, the outcomes of the ... addition to their treatment status Hence, the estimatedeffect of parental separation is the average of the typical effect of treatment on the treated only, and thedifferences in their outcomes ... on the observations whose propensity score belongs to the intersection of the supports of the propensity score of treated and controls Imposing the common support condition in the estimation of

Ngày tải lên: 12/02/2014, 11:20

... pricing on their loans along with the risk profile The nominal interest rate of the loan is one of the loan characteristics recorded in the STBL The respondent banks also indicate whether the interest ... the identity and location of the institution, the date the institution received TARP funds, and the amount of the funds received None of the banks which we identified as “non-TARP” banks as of ... U.S.A The views in this paper are solely the responsibility of the author(s) and should not be interpreted as reflecting the views of the Board of Governors of the Federal Reserve System or of any

Ngày tải lên: 16/02/2014, 10:20

... pre-cluding analysis of the effect of these concentrations on the chaperone activity of aB-crystallin (Fig 4B,C) None of the compounds had an effect on the morphol-ogy of the amyloid fibrils formed ... concentrations of Arg-HCl in the absence of the chaperone precluded analysis of the effect of this concentration on the protective ability of aB-crystallin Reduction-induced aggregation of the insulin ... against this target protein The effect of Arg-HCl on the structure and assembly of aB-crystallin We investigated whether the effects of these additives on the chaperone action of aB-crystallin were

Ngày tải lên: 18/02/2014, 16:20

... residues, only Arg213 is in the vicinity of the active site of RTA In order to establish the role of arginyl residues around the active site of RTA in the binding of rRNA, a number of RTA variants have ... and, as such, these mutants could not be purified To assess the effect of substitutions made at each of the arginyl residues on catalytic activity of RTA, the ability of each of the purified RTA ... of the hexanucleotide is at the bottom left and the target adenine is at the top of each model The sidechains of RTA are shown in blue (A) Model 1 has the tetraloop bound in the active site of

Ngày tải lên: 19/02/2014, 12:20

... of the neutral protease from Bacillus stearothermo-philus, including the position of the disulfide bridge in mutant G8C ⁄ N60C The model was created on the basis of the X-ray structure of thermolysin ... of G8C⁄ N60C and pWT In contrast to previous studies on the kinetics of thermoinactivation [13,24], this approach allows quantification of the contribution of Fig 1 Model of the 3D structure of ... indicator for the occurrence of the intermediate in the unfolding Calcium ions are the main contributory factor to enzyme stability The binding of calcium ions seems to be the main source of stabilization

Ngày tải lên: 19/02/2014, 17:20

... 2 Who does trust others? The thesis is structured as follows: The first chapter gives an overview of the existing literature and the current state of research The concept of social trust is ... percent of the population of a country answer the trust question in the affirmative They are obtained from the five waves of the World Values Survey (WVS) conducted between 1981 and 2007 The WVS ... 1981 and the Barometers These are today the main sources for researchers within this area and the number of countries for which the data is available rises every year Yet, the validity of the trust

Ngày tải lên: 20/02/2014, 16:20

... the DNA-binding activity of Mhr1 in the absence of Mg2+, because the DNA-binding activities of other HP proteins are often increased by the addition of Mg2+ in vitro [31,32] In the presence of ... presenceof the W71F or W165A mutants was not due to defects in the DNA-binding activities of these mutants DNA-binding activities of Mhr1 mutants To examine the effects on DNA-binding activity of ... in the DNA These investigations should include the identification and characterization of the DNA-binding regions and the binding modes of each of these mole-cules In this study, we analyzed the

Ngày tải lên: 06/03/2014, 09:22

... interactions (Fig 4D) Effect of the M100K mutation on the stability of the native state The effect on protein stability upon introduction of a Lys ligand to the heme-iron has been assessed for the M100K ... to the heme-iron of 2.01 A˚ and a H19 Ne2-iron-Sd M100 angle of 175
H19 is further stabilized by a H-bond between the Nd1 atom and the carbonyl oxygen of P37 In the wt struc-ture the Sdatom of ... orientation of K97 in the two structures is different In the wt structure the side chain slithers along the side of the protein surface, whereas in the M100K structure it points out into the solvent

Ngày tải lên: 07/03/2014, 17:20

... evaluations of ‘other’ designs Sixteen out of the 24 reviews explored the teaching of ‘syntax’ Of these 16, 12 provided a conclusion about the effect of syntax teaching on the accuracy and quality of ... determine the direct effects of a study of transformational-generative grammar on the language growth of secondary school pupils The results presented show that the effects of the three years of such ... contingent upon the notion of levels of text grammar (‘above the level of the sentence’) and of word grammar (‘below the level of the sentence’) Nevertheless, our aim was to focus in the in-depth

Ngày tải lên: 10/03/2014, 05:20

... governance and the development model of the welfare state (instead of the employer‟s charity) has obscured the issue of social responsibility of business industry Until the early 90s of the 20th century, ... inequality threatens the social structure, but the damage to public health have done away up the concept of social responsibility of the business On the other hand, the response of the main associations, ... image of a product or an organization while ensuring the professional ethics of the PR industry has met the wishes of the owner business Especially in industries where the quality and price of products

Ngày tải lên: 13/03/2014, 14:20

... the deformability required for the formation of the Early protein 2 C-terminal DNA- binding domain DNA complexes of var- ious types is based not only on the rigidity of the base sequences in the DNA ... For HPV-16, the flexibility of the loop may alter the adaptability of the DNA- binding helices, thus modulating the discrimination of specific versus nonspecific DNA sequences. For both the E2s, we ... flexibility. This behavior underlines a greater degree of com- pactness of the b-barrel in the HPV-16 domain than in the BPV-1 domain. Papillomavirus E2 DNA- binding domain simulations M. Falconi et al. 2388...

Ngày tải lên: 16/03/2014, 10:20