dep including enterohaemorrhagic ehec shiga toxin e coli stec

... sequences, have relied on the presence of known DNA sequence adjacent to the unknown region to be isolated Sometimes the only available sequence data for a target gene are peptide sequence data ... sequencing of the peptide fragments Degenerate primers can then be designed by back-translation of the peptide sequence into all possible coding sequences One of these nucleotide sequences will ... share considerable DNA sequence identity Since vectorette PCR is based on the use of a sequence-specific primer together with a vectorette-specific primer one often observes coamplification of other...

Ngày tải lên: 25/10/2013, 22:20

... mixture were preincubated at 30 °C for before addition of enzyme Kinetic parameters were determined as described in Experimental procedures Values of relative activity are presented as percentage ... highly conserved isoleucine residue (Ile236 in LDHB), which is replaced by valine in LDH Despite the high level of resemblance at the sequence level, the kinetic and allosteric properties of LDH ... impedes the change to the active conformation of the tetramer at higher pH Experimental procedures Chemicals DEAE–Sepharose Fast Flow, Blue Sepharose CL-6B and Superdex 75 were obtained from Amersham...

Ngày tải lên: 16/03/2014, 05:20

... undefined neighboring loci, which may exert primary effects [90] Therefore, functional studies of the biological effects of different HLA alleles are needed to determine the true effects of these ... (TGF-β1), the two main Treg cytokines that suppress the inflammatory response, has been found to be deeply deregulated in SLE patients, so they have been considered essential elements in the etiopathology ... synapses, vitamin D receptors and other immune related genes Two opposite hypothesis are currently under investigation One of them (hygiene hypothe‐ sis) discusses whether the reduced exposure to...

Ngày tải lên: 17/03/2014, 21:20

... the presence of the flagellar H7 gene The PCR primers for H7 were previously described by Gannon et al [18] Oligonucleotide sequence of the primer and expected sizes were listed in Table The ... was detected from chicken rinsing samples The detection rates of E coli O157:H7 by the three different methods were different (Table 3) In conventional method, seven isolates were obtained through ... would be useful to detect E coli O157:H7 from various sources The detection rates of E coli O157:H7 were variable among countries examined and detection methods they applied The prevalence of E coli...

Ngày tải lên: 07/08/2014, 18:21

... 30 Percent identities between sequences of Ehrlichia chaffeensis 16S rRNA gene fragment is shown as the upper matrix The lower matrix shows the number of nucleotide differences 1, EC-PGHL Korea, ... Phylogenetic analysis also revealed that the sequence of E chaffeensis EC-PGHL clustered closely on the same branch with the USA isolates These observations suggest the possibility that E chaffenesis ... with the sequences of 20 representative E chaffeensis strains available in the GenBank database showed that EC- Recenty, advances within molecular methods have made it possible to detect fastidious...

Ngày tải lên: 07/08/2014, 18:21

... Table Details of bacterial species-derived DNA sequences identified in each patient* Patient Total number of bacterial DNA sequences DNA sequences identified in each patient 38 × Escherichia coli, ... patient set from whom bacteria were detected aAccession number of the bacterial species in the database bLength of alignment on which the 16S rDNA inserted sequence and the corresponding sequence in ... patients with arthritis does not necessarily reflect the presence of complete bacterial genome, the presence of infectious bacteria or the potential of bacterial replication, or indicate whether...

Ngày tải lên: 09/08/2014, 10:23

... change in the presence of TMP Small changes were noted from experiment to experiment however none of these effects were significant (Figure 6C) We conclude, therefore, that TMP is not interfering ... activity determined in cell lysates Each experiment was performed at least times and representative experiments are shown Asterisks indicate significant differences between ligand treatments and ... (Adobe) was used to analyze images and determine mean fluorescence intensity for the nuclear region of 120 cells at each time point for each variable (20 cells from two fields from three independent...

Ngày tải lên: 11/08/2014, 03:20

... the manufacturer's recommendations The nucleotide sequences were determined with the ABI PRISM 3100 Genetic Analyzer (Applied Biosystems) The sequences were merged into one contig using the programme ... manure, soil and silage were placed in a separate bottle, 10 mL of sterile physiological saline were added and these samples were prepared by the same procedure as the biogas samples (See previous ... pasture by the seashore For these farms, only three soil samples were taken around the cattle house and eleven soil samples were taken from the common pasture All samples were collected during the...

Ngày tải lên: 12/08/2014, 18:22

... the cluster are represented by a heatmap The highest levels of expression are indicated by the brightest shades of red, while lower expression levels are represented by progressively brighter ... differential expression 'noise' due to environmental differences Wound tissues were harvested at either 30 minutes to identify immediate early genes, hours for early tissue repair effector genes, ... for example, which chemokines might be key attractive cues by revealing which of the chemokine receptors are expressed by these cells Of most therapeutic significance are those genes expressed...

Ngày tải lên: 14/08/2014, 14:21

... the difference between target gene and housekeeping gene The ∆∆Ct is then subsequently obtained by subtracting ∆Ct of treated sample from that of calibrator sample, i .e the untreated sample The ... lymphocyte activation Chemically different antigens stimulate different types of immune response TcRs recognize antigens only when they have been ingested, degraded and presented on the surface of ... present and confirmed through biochemistry by a combination of suppressed TSH and elevated free T4 In early and recurrent Graves’ disease, T3 may be secreted in excess before T4 is elevated Therefore,...

Ngày tải lên: 07/10/2015, 09:55

... known xenoestrogens (such as alkylphenol-ethoxylates and ethinylestradiol) Upon binding an active ligand, the occupied receptor then binds to the estrogen-response sequences (EREs) and interacts ... other transcriptional components to modulate gene transcription This causes expression of the reporter gene Lac-Z, and the enzyme produced (β-galactosidase) is secreted into the medium where it ... possible to significantly lower EE2, E2 , E1 , and APE concentrations for the secondary treatment water and for ozone treatment water in the sewage treatment plants than for the inflow water (Figure3...

Ngày tải lên: 05/09/2013, 08:40

... reference, demonstrative reference and comparative reference - Personal reference is by means of the personal pronouns, possessive pronouns and possessive identifiers - Demonstrative reference ... opening speeches in English and Vietnamese (2008) However, her research only focused on formal opening speeches which were well-prepared in written text and read by the speechmakers There has been no ... They are superlative adjectives, adjectives preceded by an intensifier and extreme adjectives tabulated in the following table: Table 4.3: The Use of Adjectives Expressing Extreme Meaning Adjectives...

Ngày tải lên: 26/11/2013, 12:41

... an operator stands before subject It is device used to reduce the length of the sentence and to emphasize the important factor theme in the sentence Inversion is governed much by the sentence structure ... asked any question? Wh – questions are formed by the Q-element which comes first in the sentence Then there come the operator and the subject except when the Qelement is subject [10] Where did you ... initial here carries more stress than here placed after the verb There is usually a different in meaning between Tom comes here and Here comes Tom Whereas the former means that it is habit to come to...

Ngày tải lên: 11/12/2013, 23:48

... included in the imperative, the tone of the sentence is softened The sound of the imperative sentence is more courteous and less demanding Even when the speaker or writer is aware that he or she ... they may say: Let not the dog be beaten Besides, some other mistakes Vietnamese learners often get are: imperative with 72% To emphasize in imperatives, they use “please” instead of “do” preceding ... a request You can also use the imperative to make a request, but you should use a polite word before the verb For example: Please take a seat Please wait here 26 Please hold the line Please don’t...

Ngày tải lên: 11/12/2013, 23:51

... the Vietnamese learners also ask their teachers or their friends who learn better than them This way makes progress in their studies effectively Even if they can ask the native people because ... the tenses in the subjunctive mood clause not depend on the adverb of time in the sentence However, the adverb of time is one of the sign for the learners to realize the kinds of subjunctive ... they use the mixed type conditional There are two mixed types of sentences of unreal condition In the first of these, the condition refers to the past and the consequence refers to the present...

Ngày tải lên: 11/12/2013, 23:53

... solicited in vain Executives have been given increased authority, increased pay One executive reported a large increase in salary because he applied these truths Another, an executive in the Philadelphia ... achievements Wave after wave of laughter swept over the audience Few professional speakers could have equaled his performance The next speaker, Godfrey Meyer, was a gray-headed banker, the father ... he wrote letters and poems ridiculing people and dropped these letters on the country roads where they were sure to be found One of these letters aroused resentments that burned for a lifetime...

Ngày tải lên: 24/01/2014, 00:20