cytotoxicity of silver nanoparticles against mt 2 cells

... (CC50) of silver nanoparticles was defined as 3.9 ± 1.6 mg/mL against HeLa-CD4-LTR-b-gal cells, as 1.11 ± 0.32 mg/mL against human PBMC, and 1.3 ± 0.58 mg/mL against MT-2 cells Range of antiviral ... media Cells, HIV-1 isolates, and antiretrovirals HeLa-CD4-LTR-b-gal cells, MT-2 cells, HL2/3 cells, H9 cells, TZM-bl cells, HIV-1IIIB, HIV-1Bal, HIV-1BCF01, HIV-196USSN20, AZT, Indinavir, 118-D-24, ... effect of silver nanoparticles. Nanotechnology 2005, 16:2346-2353. 15 Shahverdi AR, Fakhimi A, Shahverdi HR, Minaian S: Synthesis and effect of silver nanoparticles on the antibacterial activity of...

Ngày tải lên: 11/08/2014, 00:22

... nanoparticles 1.11 ± 0.23 (4.4%) 2.12 ± 0.12 (4.2%) 4.34 ± 0.20 (4.3%) Table 2 The extent of Zn 2+ released in cell culture medium from pure and Al-doped ZnO nanoparticles. Trang 6Figure 4 Cytotoxicity ... ionization of ZnO nanoparticles26,27 Al-doping increased cytotoxicity of ZnO nanoparticles MCF-7 cells were exposed to pure and Al-doped ZnO nanoparticles at the concentrations of 25, 50 and ... co-expoexpo-sure of NAC, abolished almost fully the cell viability Sample Amount of Zn 2+ ions released, mean ± SD (%) 25 μg/ml 50 μg/ml 100 μg/ml Pure ZnO nanoparticles 2.16 ± 0.17 (8.7%) 4.22 ± 0.21 (8.4%)...

Ngày tải lên: 08/11/2022, 15:00

... Phytotoxic effect of nano particles on rice root cells was observed by (Harajyothi and Ahmed 2011; Seif et al., 2011; Salama 2012; Aghajan et al., 2013; Mirajani et al., 2013 and Mazumdar 2014) reported ... 15d (0.20 cm) In case of P fluorescens, PF-2 cell suspension had significantly highest lesion length (2.54 cm) compared with PF-2 nano viz., PF-2 N-5d (0.08 cm), PF-2 N-10d (1.33 cm), PF-2 N-15d ... al., 2014) reported efficacy of silver bionano particles prepared using Agaricus bisporus against R solani (Elgorban et al., 2015) reported inhibitory effect of silver nano on the growth of R...

Ngày tải lên: 21/05/2020, 21:10

... detected inside cells These results were 0.0 0.5 1.0 1.5 2.0 2.5 3.0 A LPS 30 LPS 278 LPS Ag [ng/cm 2 ] Ag-NP 0 5 10 15 20 LPS 30 LPS 278 LPS 0 C Ag [ng/cm 2 ] Ag-NP LPS 0.22 LPS 2.2 LPS 22 LPS B 0.0 ... 0.22 LPS 2.2 LPS 22 LPS 0 TN 0 10 20 30 40 50 Fold change in gene expression AgNO3[mM] 24 hours AgNO3 (2) *** (1) **** (1) *** (2) **** 0 50 100 150 200 250 LPS 0.22 LPS 2.2 LPS 22 LPS 0 TN AgNO3[mM] ... measured 4 h (grey bars) and 24 h (black bars) after exposure Cells were exposed to 30 ng/cm2and 270 ng/cm2Ag-NP (A) and equal concentrations of 0.22 mM and 2.2 mM as well as 22 mM AgNO 3 (B) As positive...

Ngày tải lên: 02/11/2022, 10:39

... nanoecotoxicology Front Nanosci 2014;7:127–56. 21 Schirmer K Chapter 6‑mechanisms of nanotoxicity Front Nanosci 2014;7:195–221. 22 Wigginton NS, Wigginton NS Binding of silver nanoparticles to bacterial ... Germany) [26] The values were presented as percentage of controls, and were plotted as a function of measured total silver and cell-associated silver [26] RTgill-W1 cell culture and exposure of cells ... Environ Sci Technol 2011;46:1119–27. 6 Park E‑J, Yi J, Kim Y, Choi K, Park K Silver nanoparticles induce cytotoxicity by a Trojan‑horse type mechanism Toxicol In Vitro 2010;24:872–8. 7 Fleischer...

Ngày tải lên: 04/12/2022, 14:56

... NC021 12.892 3.2547 5.2169 47.859 Trang 10Toxicity of modified ZnO NP to CD4 T cells and Hut 78 cancer cellsTrend lines depicting Trang 11Role of surface charge/ZP of ZnO NPTrang 12Role of hydrodynamic ... Hydrodynamic size (nm) Trang 21Catalytic activity of ZnO NP in darkTrang 22Zeta Potential-60 -50 -40 -30 -20 -10 0 10 20 30 40 50 Trang 230.02 0.04 0.06 0.08 0.10 0.12 0.14 0.20 ... Physics Letters, 2007, 90, p 213902. 3 Hanley, et al, Preferential killing of cancer cells and activated human T cells using zinc oxide nanoparticles Nanotechnology, 2008 19: p 295103. 4 Hanley,...

Ngày tải lên: 05/05/2014, 23:01

... supernatant in the well of the positive control f) Cytotoxicity of silver nanoparticles against MT-2 cells The cytotoxicity of the nanoparticle preparations against MT-2 cells was determined using ... 2-fold dilutions of the solutions were added to cultures of target cells (2 × 105 MT-2 and 2 × 105 cMAGI HIV-1 reporter cells with 0.2–0.5 multiplicity of infection (m.o.i) of HIV-1IIIB virus) ... in plasmon resonance of individual colloidal silver nanoparticles Journal of Chemical Physics 2002, 116:6755-6759. 29. Petit C, Lixon P, Pileni MP: In situ synthesis of silver nanocluster in...

Ngày tải lên: 11/08/2014, 00:22

... Cytotoxic effect of Cd 2+ ion As cytotoxic effects of cadmium-based QDs are often attributed to Cd2+ ion release, the cytotoxicity of Cd2+ ions to Caco-2 cells was first investigated Cells were incubated ... trillion by 2012, so the likelihood of exposure to syn-Published: 23 October 2008 Journal of Nanobiotechnology 2008, 6:11 doi:10.1186/1477-3155-6-11 Received: 16 March 2008 Accepted: 23 October 2008 ... experienced when QDs were dosed to cells (50, 25, 12.5 and 6.25% volume of Attached live and dead Caco-2 cells after 24 hr incubation with (A) 0.84 nmol/ml, (B) 4.2 nmol/ml, (C) 21 nmol/ml and (D) 105 nmol/ml...

Ngày tải lên: 11/08/2014, 00:22

... 1350 1650 1950 2400 3000 3600 1/cm 42.545 47.550 52.555 57.560 62.565 67.570 72.575 77.580 82.585 87.590 %T 3240.52 3082.35 2887.53 2737.08 2306.94 1912.48 1782.29 1186.26 1073.42 2 A Mobility ... in 20 30 40 50 60 70 80 -200 0 200 400 600 800 1000 1200 1400 1600 1800 2 Thita Degrees 17.93 nm 19.26 nm 15.19 nm 23.25 nm 311 220 200 111 AgNPs EDX spectrum showed higher percentage of silver ... patterns of silver nanoparticles indicate that the struc-ture of silver nanoparticles is face-centered cubic (fcc) (Fig 4) In addition, the diffraction peaks at 2A values of 23.25, 15.19, 19.20, 17.93...

Ngày tải lên: 02/09/2015, 13:46

... http://dx.doi.org/10.1016/j.jare.2015.02.007 2090-1232 ª 2015 Production and hosting by Elsevier B.V on behalf of Cairo University. Trang 2Accepted 27 February 2015Available online 9 March 2015 Keywords: Silver nanoparticles ... Surf B 2010;76:50–6 [34] Veeraputhiran V Bio-catalytic synthesis of silver nanoparticles Int J Chem Tech Res 2013;5(5):255–2562 [35] Dwivedi AD, Gopal K Biosynthesis of silver and gold nanoparticles ... of synthesis of silver nanoparticles.Fig 2 Protocols employed for synthesis of nanoparticles (a) bottom to top approach and (b) top to bottom approach Fig 3 Protocol for synthesis of silver nanoparticles...

Ngày tải lên: 13/01/2020, 05:09

... treatment 1.22abcd 23.17 (28.73)de 19.70 (26.35)e T 2 : Injection method 1.66abc 23.67 (29.00)d 24.40 (29.60)ab (32.27)a 25.40 (30.26)a T 4 : Soil treatment 1.08abcde 28.17 (32.01)abc 24.20 (29.47)abcd ... Seedling root dip treatment (32.14)ab 24.25 (29.53)abc (27.35)ef 19.00 (25.84)ef Trang 9Table.2 Effect of different methods of application of green synthesized silver nanoparticles on total phenol, ... synthesis of silver nanoparticles Trang 3Synthesis of silver nanoparticles from culture filtrates of T asperellum Synthesis of silver nanoparticles was done by standardized method (Kaman, 2016)...

Ngày tải lên: 14/01/2020, 01:33

... Policy 2012; 33: 382-386 20 Nikolaev YuA, Plakunov VK Biofilm - “City of Microbes” or an Analogue of Multicellular Organisms? Microbiology 2007; 76: 125-138 21 Potera C Antibiotic resistance: Biofilm ... the nanoparticles to comply with safety requirements [5,24] Many studies indicated AgNPs-induced cytotoxicity in various types of human cells and tissues, including the oral cavity [5,25,26,27,28] ... conditions. Received: 2016.04.29; Accepted: 2016.07.27; Published: 2016.09.27 Abstract Objectives: In dentistry, silver nanoparticles (AgNPs) have drawn particular attention because of their wide...

Ngày tải lên: 15/01/2020, 11:50

... diffractogram of synthesized AgNP’s showed distinct diffraction peaks at 25.46°, 31.90°, 37.15°, 37.90°, 38.73°, 48.23°, 54.07°, 55 26°, 62.34°, 62.87°, 68.95°, 70.45°, 75.22° and 76.20° indexed ... the spectrum of the bacteria mediated silver nanoparticles exhibited transmittance band at 3259 cm-1 represents the presence of O-H free bond, aldehyde C-H stretching (2953 cm-1and 2920 cm-1) Peak ... at a voltage of 40 kV and a current of 30 mA with Cu Kα1 radiation (Sadhasivam et al., 2012) Scanning Electron Microscopy (SEM) The particle size and morphology of the silver nanoparticles...

Ngày tải lên: 17/03/2020, 20:11

... Complementary Medicine 2: 285-299 Mahendra R, Alka Y, Aniket G, 2009 Silver nanoparticles as a new generation of antimicrobials Biotechnology Advances 27: 76–83 Murugkar A D, Subbulakshmi G, 2005 Nutritional ... formation of silver nanoparticles of varying shape and size (Sudhakar et al., 2014)and can be attributed to excitation of surface plasmon resonance (SPR) peaks of the nobel metal nanoparticles ... nature of silver nanoparticles was confirmed by XRD spectroscopy (Fig 5) Diffraction patterns at 2Ø values 38.023, 44.6113, 46.093, 68.483 and 67.523 indicated the reflections of metallic silver...

Ngày tải lên: 21/05/2020, 20:03

... a 19 3.6 µg L −1 SDS 27.0 ± 1.4 20 8.8 mg L −1 SDS 21.6 ± 7.9 21 35.2 mg L −1 SDS 23.5 ± 3.3 22 11.8 mg L −1 SLS 2.7 ± 0.6 23 8.8 mg L −1 SDS; 20 mg L −1 NaSH 6.0 ± 0.8 24 20 mg L −1 NaSH; 8.8 ... to a size of 250 μm Procedure for formation of Ag 2 S nanoparticles The synthesis method of Ag2S nanoparticles was adapted from Xiang and coworkers [29] and Brelle and cowork-ers [30] Silver nitrate ... trans-formed into Ag2S [21] The Ag2S nanoparticles of size of 77.1 ± 56.8 nm were synthesized from l-cysteine and sil-ver nitrate The immobilized DMC sequestrated 24.1 % of Ag2S NPs (line 10) corresponding...

Ngày tải lên: 29/05/2020, 14:27

... 17 CHAPTER 2: MATERIALS AND METHODS 19 2.1 Overview of experimental design 19 2.2 Preparation of AgNPs 20 2.2.1 Synthesis of AgNPs 20 2.2.2 Characterization of AgNPs 21 2.2.2.1 Physicochemical ... properties 21 2.2.2.2 Evaluation of the antimicrobial activity of AgNPs 22 2.2.2.3 Determination of the cytotoxic effect of AgNPs on NIH 3T3 cell 24 2.3 Preparation of CM and effect of CM on NIH ... solution was stored at 4 ºC for future use Trang 2921 Figure 2.2 Schematic procedure of AgNPs synthesis 2.2.2 Characterization of AgNPs 2.2.2.1 Physicochemical properties Synthesized AgNPs...

Ngày tải lên: 04/09/2020, 08:39

... AgNPs 20 2.2.2 Characterization of AgNPs 21 2.2.2.1 Physicochemical properties 21 2.2.2.2 Evaluation of the antimicrobial activity of AgNPs 22 2.2.2.3 Determination of the cytotoxic effect of AgNPs ... of silver nanoparticles and bio-factors for wound healing 17 CHAPTER 2: MATERIALS AND METHODS 19 2.1 Overview of experimental design 19 2.2 Preparation of AgNPs 20 2.2.1 Synthesis of AgNPs 20 ... 3T3 cell 24 2.3 Preparation of CM and effect of CM on NIH 3T3 migration in vitro 26 2.3.1 Preparation of CM 26 2.3.2 Effect of CM on NIH 3T3 migration - Scratch assay in vitro 26 Trang 52.4.1 Deep...

Ngày tải lên: 27/10/2020, 19:58

... 1 251 ± 50 250 ± 50 254 ± 50 251 ± 50 254 ± 50 3 285 ± 10 282 ± 10 284 ± 10 282 ± 10 286 ± 10 6 318 ± 10 305 ± 10 308 ± 10 302 ± 10 318 ± 10 9 349 ± 10 332 ± 10 335 ± 10 336 ± 10 350 ± 10 12 ... concentrations for 24, 48, or 72 h (treated cells) All cells were tested and authenticated in March 2011 and again tested in June 2012 In vitro experimental studies Cell viability (MTT) assay MCF-7 cells ... 25 cm2flasks in triplicate The flasks were set up for controls and differ-ent asiaticoside concdiffer-entrations (0.0025, 0.01, 0.02, 0.04, 0.1, 0.2, 0.25, 0.3, 0.5, 1, 10, 20, 40, 50, 125, 250...

Ngày tải lên: 05/11/2020, 00:55

... 17 CHAPTER 2: MATERIALS AND METHODS 19 2.1 Overview of experimental design 19 2.2 Preparation of AgNPs 20 2.2.1 Synthesis of AgNPs 20 2.2.2 Characterization of AgNPs 21 2.2.2.1 Physicochemical ... properties 21 2.2.2.2 Evaluation of the antimicrobial activity of AgNPs 22 2.2.2.3 Determination of the cytotoxic effect of AgNPs on NIH 3T3 cell 24 2.3 Preparation of CM and effect of CM on NIH ... solution was stored at 4 ºC for future use Trang 2921 Figure 2.2 Schematic procedure of AgNPs synthesis 2.2.2 Characterization of AgNPs 2.2.2.1 Physicochemical properties Synthesized AgNPs...

Ngày tải lên: 06/02/2021, 19:38

... 17 CHAPTER 2: MATERIALS AND METHODS 19 2.1 Overview of experimental design 19 2.2 Preparation of AgNPs 20 2.2.1 Synthesis of AgNPs 20 2.2.2 Characterization of AgNPs 21 2.2.2.1 Physicochemical ... properties 21 2.2.2.2 Evaluation of the antimicrobial activity of AgNPs 22 2.2.2.3 Determination of the cytotoxic effect of AgNPs on NIH 3T3 cell 24 2.3 Preparation of CM and effect of CM on NIH ... solution was stored at 4 ºC for future use Trang 2921 Figure 2.2 Schematic procedure of AgNPs synthesis 2.2.2 Characterization of AgNPs 2.2.2.1 Physicochemical properties Synthesized AgNPs...

Ngày tải lên: 17/03/2021, 09:00