culture based biochip for rapid detection of environmental mycobacteria

... antigen of P multocida B:2 The number of cfu in stock culture of P multocida type A were higher than that in stock culture of P multocida B:2 The stock culture of P multocida type A was, therefore, ... total of 45 ml of The P multocida B:2 BHI broth was used for preparation of LPS antigen Estimation of carbohydrate concentration of LPS antigen The amount of carbohydrate was estimated for ... standardized for a type of P multocida B:2 antigen For the ascites of unrelated monoclonal antibody, the dilution of the ascites fluid was kept similar to the dilution of HS-1 Mab for that type of P

Ngày tải lên: 14/01/2020, 03:32

... Molecular-based techniques are useful for detection and genotyping of EG-complex hydatid cysts Conven-tional PCR assays were developed and evaluated for detection of CE [1, 36, 37] However, most of ... reaction mix 1.0 μl of fluorochrome dye was used for real-time monitoring 1.0μl of detection dye was used instead for detection of color change as ob-served by the naked eye 1.0μl of Bst DNA polymerase ... cycles of 95 °C for 1 min, 54 °C for 30 s and 72 °C for 45 s, and a final incubation at 72 °C for 10 min Thermal profiles were performed on a Techne TC-412 thermal cycler (Techne, Staffordshire,

Ngày tải lên: 24/11/2022, 17:48

... Physicochemical properties of different nanoforms of carbon In this section we look at the state of art of the physicochemical characterization of various carbon nanoforms First we will go through ... characterization of the carbon-based nanomaterials should report (i) structural, high resolution information (ii) amount and location of left over seed (often metal) particles and (iii) surface chemistry of ... reactive dandling bonds of the nD Trang 23 surface The mass of hetero-atoms (H, O, N) may be up to 10 – 14 % out of the total mass of the particle Oxygen is often the main component of the surface groups

Ngày tải lên: 26/07/2023, 07:40

... specific for Ara h 1 with no cross-reactivity The developed assay allowed the quantification of Ara h 1 between 25 and 200 g/ml with a limit of detection of 14 g/ml For the detection of Ara h ... sensitivity of the immunomagnetic bead assay for the detection of Ara h1 ……….……… 55 Figure 3.13 The specificity of immunomagnetic bead assay against various nuts and seeds……… ……… 56 Figure 3.14 Detection ... only 21.5% of peanut-allergic individuals experiencing resolution of this type of food allergy with increasing age [15] Thus, lifelong vigilance is essential for the majority of bearers of peanut

Ngày tải lên: 05/09/2017, 16:00

... last fifteen years, the detection of the activity of microbial enzymes, including sialidases, in vaginal fluid has been demonstrated to be useful for the rapid diagnosis of BV (Wiggins et al., ... enriched and Gardnerella selective agar) for the isolation of Gardnerella vaginalis and Neisseria spp (Thermo Fisher Trichomonas rapid test for the detection of Trichomonas vaginalis (Seisuki Diagnostics ... results showed a low sensitivity of BVBlue test (39.6 %) for the detection of Nugent’s score higher than four, indicative of altered vaginal flora and suggestive of bacterial vaginosis Similar

Ngày tải lên: 29/05/2020, 10:57

... L Open AccessEffects of an interactive mHealth innovation for early detection of patient-reported symptom distress with focus on participatory care: protocol for a study based on prospective, ... because of the alert system, transfer of information and generation of alerts, and lastly the content and delivery of the self-care advice We believe that the interaction between health care professionals ... assessment of the occurrence, frequency and distress level of symptoms, 2) a web interface for the healthcare providers, for monitoring patients’ data in real time, 3) an alert function, based on

Ngày tải lên: 06/08/2020, 06:11

... peculiarities of POFs, that have increased their popularity and competitiveness for telecommunications, are exactly those that are relevant for optical fiber based sensors For the detection of Fe(III) ... in its immobilized form, as self-assembled monolayer on the gold layer surface of the POF sensor The results showed that the DFO-SAM-POF-sensor was able to sense the formation of the Fe(III)/DFO ... in the development of new, simple and selective sensors for metal ions with the innovative SPR detection based on POF 2 Material and Methods 2.1 Reagents All chemicals were of analytical grade

Ngày tải lên: 01/11/2022, 08:49

... technically revised Annexes A and B form a normative part of this Interntional Standard Annex C is for information only Trang 5Introduction Because of the large variety of food and feed products, this ... 5.1 General For current laboratory practice, see ISO 7218 5.2 Culture media and reagents NOTE Because of the large number of culture media and reagents, it is considered preferable, for clarity, ... 9.3.1 Transfer 0,1 ml of the culture obtained in 9.2 to a tube containing 10 ml of the RVS broth (5.2.2); transfer 1 ml of the culture obtained in 9.2 to a tube containing 10 ml of MKTTn broth (5.2.3)

Ngày tải lên: 07/03/2014, 16:20

... Q-value of the previousstate and the performed action according to the reinforcement and the value ofthe next state The agent receives a constant negative reinforcement for eachaction it performs ... nature of the reinforcement learning algorithm poses the cessityof extracting discrete values from the sensor signals of force and posi-tion This feature extraction process along with the basis of ... training of the mapand not in our scheme SOMs perform a nonlinear projection of the probabilitydensityfunction ofthe input space onto the two-dimensional lattice of units Though all the six Trang 8force

Ngày tải lên: 28/03/2014, 14:20

... YES Skeletonization Edge Detection and Model Foreground Region Parameters Ellipse Trang 41! IMTrang 47Segmentation of Region SizeFiltering Filtering Based on Measures Region Head pixels low intensity ... Eye Detection Constraints Eye Region No Yes Trang 37(x0,y0) a b Trang 40Initial Ellipse Centroid EstimationError Computation Centroid Reestimation Convergence Test NO YES Skeletonization Edge Detection ...

4>9 Trang 16Detection Face Pattern Faces Face Face Pattern Background Pattern YES NO Trang 34Background SegmentationBackground Model Adaptation Background Template Deformable Best Face

Ngày tải lên: 24/04/2014, 14:09

... variation of H 2 S concentration in air between the values of 0% and 2%. This confirms the potential for this system to be applied not only for H 2 S detection, but also for measurements of H 2 ... malodour measurements After demonstrating the ability of the RIfS system for detection of H 2 S, the performance of our device was examined for oral malodour analysis in three volunteers with ... major presence of H 2 S that arises from bacterial metabolism of amino acids and proteins is another example of biomedical determination of H 2 S that can be used for diagnosis of specific diseases

Ngày tải lên: 18/06/2014, 22:20

... sequence information for MneRV2 consisted only of a region of the DNA polymerase which had significant sequence similarity with the macaque RV1 rhadinovi-ruses, and therefore was unsuitable for the ... compared to the sequence available for PapRV2 DNA sequence for 352 bp of the DNA polymerase gene was obtained An alignment of this sequence with the corresponding sequence of the PapRV2 rhadinovirus ... the viral load of macaque rhadinoviruses belonging to the RV2 lineage of KSHV-like rhadinoviruses The primers and probe for this assay were based on sequences within the 3' end of the ORF 60 cod-ing

Ngày tải lên: 18/06/2014, 22:20

... role of Graphiti [21] is to support the creation of algorithm and architecture graphs for the proposed framework Graphiti can also be quickly configured to support any type of file formats used for ... graph (types of vertices and edges, and attributes allowed for objects of each type); (2) the visual syntax of the graph (colors, shapes, etc.); (3) transformations from the file format in which ... three trans-formation steps: the hierarchy flattening transtrans-formation, the HSDF transformation, and the DAG transformation (seeSection 3.3.2) These transformations prepare the graph for the

Ngày tải lên: 21/06/2014, 20:20

... probability of detection approximately 73% for probability of false alarm 4% [33]. For the same probability of false alarm, a probability of detection, which falls between 80.95% for rectangular ... probability of detection for vocal fold paralysis in men was measured, that is approximately equal to 90% for probability of false alarm 10%. The gain in the probability of detection for vocal ... Option for the Detection of Vocal Fold Paralysis and Vocal Fold Edema Constantine Kotropoulos (EURASIP Member) 1, 2 and Gonzalo R. Arce 2 1 Department of Informatics, Aristotle University of Thessaloniki,

Ngày tải lên: 21/06/2014, 20:20

... the detection of the max-ima is that most of the brightest points are mapped to a value of 255 This clipping is unavoidable, as high-reflective sur-faces are involved Hence, for the detection of ... [5] The principle of all these methods is the combination of three-dimensional information obtained by one or more of calibrated cameras with information depicted in disturbances of the projected ... that the knowledge of the projected pattern combined with the observed deformation of the structure on the object surface permits the retrieval of accurate depth in-formation of the scene [2] This

Ngày tải lên: 22/06/2014, 19:20

... the known value of the constituent of interest for sample j, and Npis the number of samples in the prediction set The ratio of performance to deviation (RPD: ratio of the SD of the reference ... of prediction; RPD: ratio of performance to devia-tion SECV SEL SEP Number of PLS terms RPD Table V Statistics for equations formulated for the extractive-free disk samples. N: total number of ... value of 2.0–3.0 is regarded as adequate for rough screening A value of above 3.0 is regarded as satisfactory for screening (for example in plant breeding), values of 5 and upward are suitable for

Ngày tải lên: 08/08/2014, 14:20

... over 65 years of age (Table 3) Analysis of ROC also showed that the best cut-off for each subgroup remained at 4.0 (Figure 2) Figure 2 ROC curves for severity score for the detection of coronary ... performance of 3DMP, sensitivity and specificity were evaluated at different cut-offs for severity (Table 5) This comparison also showed that a cut-off of 4.0 provided the best compromise of ... to assess the performance of the prediction of stenosis independent of the prevalence of stenosis the positive and negative likelihood ratios (LR) were calculated [20] A value of P < 0.05

Ngày tải lên: 08/08/2014, 16:23

... patients Figure 2 ROC curves for severity score for the detection of coronary stenosis for different gender, age groups, and type of revascularization yoa = years of age 1 - Specificity 1,0 0,8 ... cut-offs for severity (Table 5) This comparison also showed that a cut-off of 4.0 provided the best compromise of sensitivity and specificity As the negative predictive value at a cut-off of 4.0 ... cause of death in the developed world Between 15% and 20% of all hospitalizations are the direct results of CAD [1] Revascularization of coronary arteries is one of the most frequently performed

Ngày tải lên: 08/08/2014, 17:20

... http://arthritis-research.com/content/10/6/R131 Page 1 of 10 (page number not for citation purposes) Vol 10 No 6 Research article Limited reliability of the indirect immunofluorescence technique for the detection of anti-Rib-P antibodies ... conventional HEp-2 cells substrates for the detection of anti-Rib-P antibodies and to compare different state-of-the-art diagnostic technologies for the detection of anti-Rib-P antibodies. Materials ... injection of affinity-purified anti-Rib-P anti- bodies. Thus, the detection of anti-Rib-P antibodies might be important not only for the diagnosis of SLE but also for the evaluation of CNS complications,

Ngày tải lên: 09/08/2014, 13:22

... sample Interpretation of Cut off value (RLU of specimen/mean RLU of PC) Cut-off ratio of to 0.99 is negative for HR HPV; Cutoff ratio greater than 1.0 positive for HR HPV The cut off ratio at 1.0 ... intensity of HPV infection This ratio of any specimen represents empirically a relative measure of the viral load in it Cut off value (RLU of specimen/ mean RLU of PC) was 1.0 pg/ml of sample ... of HPV viral load Ratio of relative light unit (RLU) of the sample/mean of RLU of three positive controls (PC) were taken as an estimate of approximate viral load which relates to the index of...

Ngày tải lên: 12/08/2014, 01:22