crystal structure and cation exchange properties

... enzyme with respect to its catalytic properties and crystal structure The crystal structures of ChiG and ChiC [18] permitted structural comparison between bacterial and plant enzymes, which provided ... violet) and a small, presumably amorphous, subfraction of a-chitin and b-chitin, but was not capable of degrading crystalline chitin completely The crystal structures of chitinase G and a related ... three-dimensional structure, and catalytic mechanism [5,6] Family 18 and family 19 glycoside hydrolases both contain chitinases Members of the two families have very different three-dimensional structures and...

Ngày tải lên: 07/03/2014, 11:20

... Characteristic crystals measure 29 2.2 2 Magnetic characteristic and magnetic refrigeration measurement 32 CHAPTER III: RESULTS AND DISCUSSION 33 3.1 Crystalline structure of NiMnSbB2 and NiMnSbCo1B2 ... elemental Boron and our group Cobalt In this thesis we refer to manufacturing technologies and do experiments of crystal structure, magnetic and magnetic refrigeration NiMnSbCo1B2 alloy and NiMnSbB2 ... B-site cations This results in the possibility of ordered and disordered variants Figure 1.5 Crystalline structure of Perovskite familiar ABO 3 Trang 20Figure 1.6 Comparing the crystalline structure...

Ngày tải lên: 24/06/2016, 12:58

... - Integrated space-group and crystal-structure determination Acta Crystallogr Sect A, 71, 2015a, 3-8 SHELDRICK, G.M.: Crystal structure refinement with SHELXL Acta Crystallogr Sect C, 71, 2015b, ... unidentate ligand, as a chelating ligand, as a bridging ligand, or as a monoatomic bridging ligand This causes the existence of a rich family of compounds with various structures (DEACON and PHILLIPS ... [Cu(2-Clnic)2(3,5-py)(MeOH)] The crystal and molecular structure of the complexes under study [Cu(2-Clnic)2(Bim)2] and [Cu(2-Clnic)2(FP)2] has also been studied by X-ray structure analyses Sketch and abbreviations...

Ngày tải lên: 19/03/2023, 15:26

... found in structures I and II is stable and not subject to conformational changes due to different crystal packing Quality of protein structure models The final R and Rfree factors for structure ... other through helices a1 and a5, and helices a10 (including the subsequent loop) and a11 Molecules A and B in the asymmetric unit of the pleABD/2a structure I formed a crystallographic dimer (Fig ... crystals The first microcrystals were used as seeds Crystals reached dimensions of up to 0.8 mm after 1–2 days Data collection and processing The collection and processing of X-ray data from crystal...

Ngày tải lên: 07/03/2014, 15:20

... respectively Structure determination and refinement The crystal structure of bsSHMT (1KKJ) was used as the initial model for the refinement of Y51F and Y61A bsSHMTs Water and ligand molecules ... Y61 and increases the distance between the hydroxyl group of Y61 and Ca of the ligand. In Gly, l-Ser and l-allo- Thr complexes with Y51F bsSHMT, the hydroxyl group of Y61 and Ca of the bound ligand ... structural and functional studies on Y51F, Y61F and Y61A bsSHMTs. These studies suggest that Y61 is a possible candidate for proton abstraction from Ca of Gly and 3-hydroxy amino acids, and that...

Ngày tải lên: 16/03/2014, 06:20

... b5 and b8) forms the upper part of the structure along with strands b3 and b4, which form a b meander. On the opposite side of the sheets, after the meander, is a region of six a helices and ... collected from wild-type crystals. Structure determination and refinement Data were processed and scaled using the HKL package programs denzo and scalepack [28]. The structure was solved via molecular ... the crystal structure of the activation domain); and ⁄ or (3) there is some proteolytic removal of the N-terminus during purification. Analysis of the interaction between MS activation domain and...

Ngày tải lên: 23/03/2014, 09:21

... be 1:2 metal: ligand for C1, C3 and C5 and 1:1:1 metal: ligand: 1, 10-phenanthroline for C2, C4 and C6 The analytical data of the ligand and metal complexes are given in Table 1 and are in good ... Co(III) and Ni(II) complexes and Trang 10pyramidal for VO(IV) complexes The crystal structure of ligand HL has also been determined by X-ray diffraction studies The ligand and its Co(III), Ni(II) and ... characterization, crystal structure and Hirshfeld surface analysis of Co(III), Ni(II) and VO(IV) metal complexes with a novel Schiff base ligand and their antimicrobial activities Disha Sharma a and Hosakere...

Ngày tải lên: 27/05/2020, 04:08

... single-crystal structure studies, and these intermolecular interactions in the single crystal structure of bis(2‒isobutyrylamidophenyl) amine have been visualized via Hirshfeld surface analysis and ... the solid‒state structure of H3L NNN The molecular structure, vibrational frequencies, and infrared intensities of H3L NNN were computed by ab initio HF and DFT (B3LYP, B3PW91, and BLYP) methods ... ionization (ESI) source and triple quadrupole ion trap mass analyzer by direct infusion and ESI operated in the positive and negative mode in Advanced Technology Research and Application Center, Mersin...

Ngày tải lên: 13/01/2022, 00:31

... exclusion, and cation-exchange chromatography During the screening for crystallisation conditions it turned out that microcrystals appeared already in the concentrated protein solution Crystals ... TrCel45A) and C (Phanerochaete chrysosporium PcCel45A) Furthermore, the crystal structure of MeCel45A is reported Initial rates and hydrolysis yields were determined by reducing sugar assays and product ... X-ray Trang 3diffraction data and the refined protein model are summarized in Tables S1 and S2 2.2 Structure of MeCel45A M edulis Cel45A has a compact and globular structure with approximate...

Ngày tải lên: 01/01/2023, 13:11

... confirmed by subsequent NMR [18] and crystallographic studies [19,20] Recently, crystal structures of chagasin in complex with human cathepsins L and B [20,21], and additionally with falcipain ... where Fo and Fc are observed and calculated structure factors, respectively Rfree is calculated analogously for the test reflections, randomly selected and excluded from the refinement e Ramachandran ... inhibitor concentration Crystallization All crystallization experiments were performed at 18 °C using the hanging-drop vapor diffusion method and Crystal Screens I and II and PEG ⁄ Ion Screen from...

Ngày tải lên: 16/03/2014, 04:20

... high-pressure nitrogen and methane adsorption isotherms of natural and monovalent (Li+ and Na+) cation-exchanged clinoptilolite at 0 and 25 ◦C and reported that the adsorption and kinetics characteristic ... type and distribution of the charge-balancing cations Faghihian et al.33 studied the adsorption of N2, CH4, and C2H6 on natural clinoptilolite and on its cation-exchanged forms (Na, K, and H) ... of the acid and heavy metal cation-exchange treatments on structural, thermal, and methane adsorption properties of the mordenite samples Trang 32 Results and discussion2.1 Materials and chemicals...

Ngày tải lên: 12/01/2022, 23:46

... domains of Spir-2 and PTPN13 were generated by RT-PCR and inserted into pEGFP-C1 and pGEX-4T-2 The N- and C-terminal regions of the v-KIND KIND2 and the MAP2 CD were generated by PCR and cloned into ... structural and functional properties of the KIND domains remain largely unknown Within the KIND protein family, v-KIND is unique because it possesses two tandem-repeated KIND domains, KIND1 and KIND2, ... polymerization, stability and rear-rangement of microtubules in neuronal dendrites [13–15] and is associated with some neurological and psychiatric disorders [16,17] However, the structure– function...

Ngày tải lên: 14/02/2014, 19:20

... a three-stranded antiparallel b-sheet and loops connecting these secondary structure elements The con-tents of the a-helix and b-strand were 50% and 11%, respectively The monomer structure comprised ... enzymes were higher (Table 1), the crystallographic studies were per-formed on apo- and metal-reconstituted ApeSODs Crystallization and determination of structure The crystals of ApeSOD were grown ... The crystal structures of the superoxide dismutase from A per-nixin the apo, Mn-bound and Fe-bound forms were determined at resolu-tions of 1.56, 1.35 and 1.48 A˚, respectively The overall structure...

Ngày tải lên: 14/02/2014, 22:20

... indexed, integrated and scaled using the programs Mosflm and SCALA in the CCP4 suite [47] with DPS [48] Structure determination and refinement The structure of the enzyme was determined and refined using ... is inactive and serves as an inhibitor for diol and glycerol dehydratases [57] Overall structure of glycerol dehydratase in complex with cobalamin and propane-1,2-diol The crystal structure of ... three bands with an Mrof 61 000 (a), 22 000 (b) and 16 000 (c) (marked with an arrowhead) were overexpressed in E coli carrying pUSI2E(GD) (Fig 2A) and progressively enriched upon purification,and...

Ngày tải lên: 21/02/2014, 03:20

... phasing model for the N structure The G and TG structures were solved using the N structure as a search model, and the TG structure was used in phasing of the T structure The structures were refined ... backbone atoms of Mglu and Bacillus glutaminase induced by ligand binding Displacement values for backbone atoms of the nonliganded and liganded structures are shown The average and SD of these conserved ... Effect of L-glutamate and Tris on backbone atoms Backbone atoms of the overall amino acid residues of the T and TG (T ⁄ TG), N and G (N ⁄ G), G and TG (G ⁄ TG), and N and T (N ⁄ T) structures are superimposed...

Ngày tải lên: 06/03/2014, 09:22

... the structures of UcPLA2 and AcPLA2 on the basis of the crystal structures of the porcine group I PLA2 (PDB 5p2p [27]) and D r russelli PLA2 (PDB 1vip [29]), respectively Structure modeling and ... 105 Fig Models of UcPLA2 and AcPLA2 UcPLA2 (A) was modeled on the basis of the crystal structure of the porcine PLA2 (PDB 5p2p) and AcPLA2 (B) on the basis of the crystal structure of D r russelli ... – its primary structure and phylogenetic classification ˇ ˇ ˇ ˇ ˇ ˇ Andrej Razpotnik1, Igor Krizaj2, Jernej Sribar2, Dusan Kordis2, Peter Macek1, Robert Frangez3, William R Kem4 and Tom Turk1...

Ngày tải lên: 06/03/2014, 11:20

... conserved strands b3 and b4 (Fig 4A, boxed), and the hydrogen bond network between residues Asp120 and Gly121 and Val74 (Fig 4A,B) [33], are also conserved in PPL2 On the other hand, the lar-gest ... brasiliensis) (PDB code 2HVM), the Fig 3 Crystal structure of PPL2 (A) and (B) show two views of the (ab)8barrel fold of PPL2 The a-helices (red) and b-strands (yellow) are labeled from 1 to 8 Disulfide ... sequence by a combination of Edman deg-radation and cDNA cloning, and report its biochemi-cal characterization and the determination of its crystal structure Our results show that this protein,...

Ngày tải lên: 16/03/2014, 13:20

... profiles, and secondary structure prediction of the 105-kDa protein, HSP90, APG-1, and OSP94 For the hydropathy profiles of APG-1 or OSP94, we used the hydrotable of Hopp and Woods The secondary structure ... residues, and those amino-acid sequences were the same To determine the interaction between the 105-kDa protein and APG-1 or OSP94, we investigated the amino-acid sequence and some biochemical properties ... centrifugation at 20 000 g for 10 min at 4C, and used for SDS/PAGE and immunoblotting as the insoluble fraction Homology search, hydropathy profile, and secondary structure prediction DNASIS(version 2.1,...

Ngày tải lên: 17/03/2014, 10:20

... SGSGSG-RATE and SGRSGRSG-RATE were successfully crystallized as described in Experimental procedures, all yielding the same crystal form The crystals were highly isomorphic, and the structures ... GPPG, SGSGSG, and SGRSGRSG, which allowed us to analyze the mode of binding of RI to the RNase A tandem enzymes Modeling studies with the crystal structures of the RI–RNase A complex and the SGRSGRSG-RNase ... binding of RI and RATEs, the crystal structures of various RATEs differing in linker sequence length and amino acid composition (GPPG, SGSGSG, or SGRSGRSG) were solved On the basis of these structures,...

Ngày tải lên: 22/03/2014, 16:21

... Previously, we compared the structure and properties of the wild-type HSP22 and its K141E mutant [29] The present study analyses the structure and properties of K137E and the K137,141E mutant of ... strands of two crystallized nonmetazoan sHsp Two predictions slightly differ with respect to the location and length of specific b-strands For example, in the model of aB-crystallin, the b7 strand ... b-strands are numbered and the b5 and b7 strands are shown in red and blue, respectively G104 (equivalent to K137 of human HSP22) and R108 (equivalent to K141 of human HSP22) are shown in purple and...

Ngày tải lên: 23/03/2014, 07:20