concluding remarks and prospects

... Plant Pathogen Detection and Disease Diagnosis: Second Edition, Revised and Expanded, P Narayanasamy Handbook of Plant and Crop Physiology: Second Edition, Revised and Expanded, edited by Mohammad ... Functional Plant Ecology, edited by Francisco I Pugnaire and Fernando Valladares Handbook of Plant and Crop Stress: Second Edition, Revised and Expanded, edited by Mohammad Pessarakli Plant Responses ... by Keith A Smith and Chris E Mullins Growth and Mineral Nutrition of Field Crops, N K Fageria, V C Baligar, and Charles Allan Jones Semiarid Lands and Deserts: Soil Resource and Reclamation,...

Ngày tải lên: 08/04/2014, 12:49

... standard and buffer I as blanks After the addition of phosphoric acid and thiobarbituric acid, samples were heated http://www.comparative-hepatology.com/content/2/1/2 at 80°C for 15 Longer and ... Belgium, and of the University of Berne, Switzerland Authors' contributions MV and JV carried out this study together with the statistical analysis FN participated in the design of the study JF and ... FWO-Vlaanderen (n° b.0111.98) to FN References Wiest R and Groszmann RJ The paradox of nitric oxide in cirrhosis and portal hypertension: too much not enough Hepatology 2002, 35:478-491 Bosch J and...

Ngày tải lên: 13/08/2014, 13:20

... screening Error-prone PCR and DNA shuffling were separately used to create a randomized library of protein variants based on the Q143A hMnSOD template One hundred and fifty thousand QC774 transformants ... cloning site of the vector by digestion with NcoI and PstI This vector backbone was excised from an agarose gel and purified For both standard and error-prone PCR amplification of hMnSOD genes, ... grant GM54903 (DS) and National Science Foundation CAREER Award Bes-0348107 (HZ) We thank Patrick Quint and Diane Cabelli for help with kinetics, and Chingkuang Tu for assistance and helpful discussion...

Ngày tải lên: 07/03/2014, 11:20

... Densitometry of the bands using the positive control nontransgenic flower pooled RNA sample as a standard demonstrated a significant difference between wild-type and Gamma plants, and wild-type and IgG plants, ... nontransformed (WT) and transformed (c and IgG) plants Lysed cell extracts were immunoprecipitated with antiserum to murine IgG (heavy and light chains), and after SDS/PAGE and immunoblotting, ... gamma chain transgenic tobacco; and IgG, kappa and gamma chain transgenic tobacco 6046 J Nuttall et al (Eur J Biochem 269) from nontransformed and transformed plants and looked for coimmunoprecipitation...

Ngày tải lên: 31/03/2014, 08:20

... identify viral and clinical factors associated with vaginal X4- or R5-like genotypes, we evaluated plasma and vaginal viral levels, ART use, and CD4+ T lymphoctye counts using Chi-square and Fisher's ... data analysis, and drafted the manuscript PK participated in the study design and conducted the statistical analyses RC participated in the design and coordination of the study and facilitated ... samples NL processed clinical samples and conducted PCR and viral load analyses AA participated in the coordination of the study, directed sample and data analysis, and assisted in drafting the manuscript...

Ngày tải lên: 20/06/2014, 01:20

... contributions HY treated the patient, analyzed and interpreted the patient data, and was a contributor in writing the manuscript NT advised on the format and design and assisted in providing a critical ... intracystic instillation of minocycline before and Changes2in serum and cyst fluid CA19-9 levelshydrochloride Changes in serum and cyst fluid CA19-9 levels before and after the intracystic instillation ... values, and serum CA19-9 levels were rapidly decreased and normalized by amelioration of inflammation [8], indicating a significant association between inflammation of the hepatobiliary system and...

Ngày tải lên: 11/08/2014, 21:22

Ngày tải lên: 29/10/2012, 11:28

Ngày tải lên: 29/10/2012, 11:28

Ngày tải lên: 29/10/2012, 11:28

Ngày tải lên: 29/10/2012, 11:28

Ngày tải lên: 29/10/2012, 11:28

Ngày tải lên: 29/10/2012, 11:28

Ngày tải lên: 29/10/2012, 11:28

... of seven a-helices, a three-stranded antiparallel b-sheet and loops connecting these secondary structure elements The contents of the a-helix and b-strand were 50% and 11%, respectively The monomer ... (B) and (C) Prepared with PYMOL [44] C of the N-terminal extended region and the following two a-helices, and the globular, (a + b)-type C-terminal domain Oligomeric structure The A ⁄ B and C ... coordinated with six ligands: the five same ligands in the Mn-reconstituted enzyme and an additional water oxygen, which, together with the OD2 of Asp165, the NE2 of His79 and the NE2 of His169,...

Ngày tải lên: 14/02/2014, 22:20

... is disturbed and the outputs are "not matched" any more (compare figures 4.17 and 4.19) Figures 4.21 and 4.22 show the output and control responses of the system with saturation and the EG operator ... frequencies and directions The saturations alter the direction and frequency of the control signal and thus interfere with the inversion process The main problem is that although both the compensator and ... observable mode of (A,C) and let the multiplicity of ki(A)) be n i The function g(x) is finite Vxe R n if and only if a) Re(Xi(A)) < 0, Vi, and b) The modes Xi(A) with Re(Xi(A)) = and n i > have independent...

Ngày tải lên: 18/02/2014, 10:20

... zinc to Nd1 of His63, His71, and His80 and to Od1 of Asp83 ˚ Lower and upper distance limits of 1.8 and 2.3 A, respectively, were imposed between the metal ions and the donor atoms Finally, 3276 ... cCH3; and 15 bCH2 were assigned by the analysis of the HNHB experiment In each subunit the metal ions were included by allowing copper to bind to Ne2 of His48 and His120 and to Nd1of His46, and ... between the imidazole nitrogen and nonexchangeable imidazole protons Structure constraints and calculations In three-dimensional 15N- and 13C-NOESY-HSQC spectra and in two-dimensional 15N-NOESY...

Ngày tải lên: 21/02/2014, 03:20

... of 417, 536 and 566 nm, which are almost identical to those of HbCO (417, 537 and 567 nm) An O2 spectrum was also recorded, and SOD–Hb again showed the typical peaks at 414, 538 and 573 nm, similar ... Sigma-Aldrich, and standardized using an extinction coefficient of 72.8 m)1Æcm)1 at 230 nm [34] The fluorescence signal was recorded for 30 at excitation and emission wavelengths of 460 and 525 nm, ... oxidative stress and human pathologies: a brief history and a future vision Biomed Pharmacother 59, 139–142 Ronda L, Bruno S, Abbruzzetti S, Viappiani C & Bettati S (2008) Ligand reactivity and allosteric...

Ngày tải lên: 07/03/2014, 00:20

... BetA Betulinic acid inhibits hepatitis B virus and PC significantly reduced HBsAg and HBeAg serum levels and HBV DNA replication Also, both BetA and PC substantially decreased SOD2 mRNA expression, ... BetA and PC specifically increased superoxide anion generation in liver tissue, but had little effect in aorta, and no effect at all in kidney and brain, indicating that both BetA-mediated and ... for SOD2 (siSOD2) alone, and the related biomedical parameters were measured As shown in Fig 6, the levels of SOD2 mRNA (Fig 6A) and SOD2 protein (Fig 6B) decreased to 33% and 46%, respectively,...

Ngày tải lên: 16/03/2014, 01:20