combined raman spectroscopy and confocal microscopy

... and Raman microscopy is used for validation The two techniques are complimentary by nature; DHM relies on the linear elastic scattering of a wave front passing through the sample, and Raman spectroscopy ... similar power and acquisition time as our study The Raman peaks observed correlate well with our study, where they have also noted Raman bands around 1455 cm−1, 1585 cm−1 and 1665 cm−1 for T and B cells; ... instrument for combined Raman spectroscopy optical coherence tomography of skin cancers Lasers Surg Med 43(2), 143–151 (2011). 9 Ashok, P C et al Multi-modal approach using Raman spectroscopy and optical

Ngày tải lên: 04/12/2022, 15:38

... Mass Transport Effect, and Surface Relief Absorption Edge, XPS, and Raman Spectroscopy Combined with Profilometry Study O Kondrat1*, R Holomb1, A Csik2, V Takáts2, M Veres3and V Mitsa1 Abstract ... be seen, the Raman spectra of g-As40S60demonstrate a broad band with the maximum at 340 cm−1and shoulders at 310 and 380 cm−1 The main band centered at 340 cm−1 is a characteristic band of symmetric ... structure The simultaneous increases in inten-sities of 360 cm−1 Raman band and bands in the re-gion of molecular and As–As valence band vibrations (100–300 cm−1) can indicate the increasing of the

Ngày tải lên: 19/11/2022, 11:48

... novel 3-D microscopy techniques rely on non-linear optical phenomena – 2-p and multiphoton microscopy – Higher harmonics microscopy (SGH, TTH) – Coherent Anti-Stokes Raman scattering microscopy ... specimens than confocal imaging Biophys J 1998 Oct;75(4):2015-24 Trang 16acid-fucsin-by confocal microscopy with 2 µW of 532-nm light (left, columns 1 and 2) and multiphoton microscopy with ... vacuolated notochord and the distribution of somites alongside the notochord Image size: (A)–(G) and (I): 235 × 235-μm 2 ; (H): 40 × 40-μm 2 Trang 234π confocal microscopy• 4π confocal microscopy was

Ngày tải lên: 21/12/2016, 10:57

... properties and the resonance Raman spectroscopy of single wall carbon nanotubes are reviewed Because of the unique van Hove ties in the electronic density of states, resonant Raman spectroscopy ... the sense that STS ning tunneling spectroscopy) [22] and resonant Raman spectroscopy exper-iments [25,27,28,29] depend on the chirality of an individual SWNT,andtherefore trigonal warping effects ... nm,1.06–1.45 nm and 0.68–1.00 nm [3,40] Thediameter distribution in each case was determined from TEM experimentsand from measurement of the RBM frequencies using Raman spectroscopyand several different

Ngày tải lên: 07/09/2020, 14:39

... Infrared and RamanSpectroscopy Vibrational spectroscopy includes several different techniques, the most important ofwhich are mid-infrared (IR), near-IR, and Raman spectroscopy Both mid-IR and Ramanspectroscopy ... IR and Raman spectra, use of numerous tablesthat are discussed in text, and finally referenced to a selection of fully interpreted IRand Raman spectra This fully integrated approach to IR and Raman ... active in both the IR and Ramanspectra For such highly symmetrical molecules vibrations which are Raman active are IRinactive and vice versa and some vibrations may be both IR and Raman inactive.center

Ngày tải lên: 10/05/2022, 14:46

... groups in di- and tripeptides and considerations on the conformation of the extended helix J Raman Spectrosc 29, 81–86 (1998). 66 Schweitzer-Stenner, R.Visible and UV-resonance Raman spectroscopy ... concentrations using a combination of atomic force microscopy (AFM), circular dichro-ism (UV-CD), X-ray diffraction (XRD) and 1D- and 2D-infrared spectroscopy (1D-IR and 2D-IR) Previous ss-NMR studies: αS ... Two-Dimensional Infrared Spectroscopy and Atomic Force Microscopy Steven J Roeters1,*, Aditya Iyer2,*, Galja Pletikapić2, Vladimir Kogan3, Vinod Subramaniam2,4 & Sander Woutersen1 The aggregation

Ngày tải lên: 24/11/2022, 17:49

... lipids, and DNA can be separately extracted from a Raman spec-trum and their quantitative variations and structural changes can be investigated Thus, biological and medical applications of Raman spectroscopy ... molec-ular level Raman spectroscopy is one of the vibrational spectroscopies Strong water bands in a Raman spectrum do not overlap with the fingerprint region (< 2000 cm−1)18–20 Thus, Raman spectroscopy ... near-infrared (NIR) and Raman spectroscopy17 The results of this study showed that the viability indices calculated by NIR or Raman spectroscopy were higher for the embryos that were implanted and resulted

Ngày tải lên: 04/12/2022, 15:32

... rared and Raman Trang 2INFRARED AND RAMAN SPECTROSCOPY Trang 3INFRARED AND RAMAN SPECTROSCOPY PRINCIPLES AND SPECTRAL INTERPRETATION SECOND EDITION P e t e r J L a r k in Spectroscopy and Materials ... active in both the IR and Raman spectra For such highly symmetrical molecules vibrations that are Raman active are IR inactive and vice versa and some vibrations may be both IR and Raman inactive. ... layer) Vibrational spectroscopy has a very broad range of applications and provides solutions to a host of important and challenging analytical problems. Raman and mid-IR spectroscopy are complementary

Ngày tải lên: 02/11/2023, 11:54

... capabilities of Raman spectroscopy and allowing it to reach its full potential.In the late 1970s, the advent of microscopic Raman spectroscopy, which combined an optical microscope with a Raman spectrometer, ... molecular structure, composition, and interactions.The Raman effect is fundamental to Raman spectroscopy and involves the interaction between a sample's electron cloud and the electric field of monochromatic ... D.E and Coleman, D.L., 1986 Clinical monitoring using Raman spectroscopy Journal of Clinical Monitoring, 2(4), pp.215- Lawson, D., Samanta, S., Magee, P.T and Gregonis, D.E., 1993 Raman spectroscopy

Ngày tải lên: 26/02/2025, 22:29

... label-free and nondestructive imaging techniques for biochemical studies, including coherent anti-Stokes Raman (CARS) microscopy, multiphoton microscopy, and confocal Raman microscopy.The confocal Raman ... Various methods, including surface-enhanced Raman spectroscopy (SERS), Raman tweezers, tip-enhanced Raman spectroscopy (TERS), and coherent anti-Stokes Raman scattering (CARS), facilitate the identification ... scientist to successfully reproduce Raman's work, helping to dispel doubts and coining the terms "Raman effect" and "Raman lines" for the scientific community.Raman spectroscopy is a non-destructive

Ngày tải lên: 28/02/2025, 22:52

... AI and Raman Spectroscopy in medical diagnostics • To select and implement appropriate AI algorithms for analyzing Raman Spectroscopy data • To collect and preprocess data using Raman Spectroscopy ... of Raman spectrometer setup using CCD detection module Excitation laser (Andrew Downes, 2024)Raman spectroscopyProfessor C.V Raman, along with K.S Krishnan, pioneered the discovery of the Raman ... compounds and biological materials The Raman effect remains one of the most adaptable and widely used methods in modern analytical science.Raman spectroscopy is a highly versatile and non-invasive

Ngày tải lên: 17/07/2025, 21:36

... preparation and handling and sometimes com-plicated modeling due to the irregular geometry involving additional structures such as a heater In this work, we describe a noncontact, micro-Raman spectroscopy–based ... expressly forbidden Terms & Conditions of access and use can be found at http://www.tandfonline.com/page/terms-and-conditions Trang 3Nanoscale and Microscale Thermophysical Engineering, 18: 183–193, ... ULTRATHIN FILMS USING MICRO-RAMAN SPECTROSCOPY Zhe Luo1, Han Liu2, Bryan T Spann1, Yanhui Feng3, Peide Ye2, Yong P Chen4, and Xianfan Xu1 1School of Mechanical Engineering and Birck Nanotechnology

Ngày tải lên: 06/05/2014, 08:54

... µ-filtered 50 mM HEPES (pH 7.5), 100 mM MgSO4, and 150 mM NaCl) and stored in aliquots in liquid nitro-gen Viral titers were determined by standard plaque assay combined with immunostaining for RSV Mice ... were stained with hematoxylin and eosin (H&E) and inflammation was assessed as disruption or denudation of the epithelial layer and infiltration of monocytes and lymphocytes into the perialveolar ... significantly attenuated AHR and inflammation, as seen by the decrease in eosinophils and lymphocytes and the reduced perialveolar damage com-pared to control group and mice treated with FP alone

Ngày tải lên: 20/06/2014, 01:20

... the M13 phage using Raman spectroscopy The observed vibrations are compared with theoretical calculations based on an elastic continuum model and appropriate Raman intensities and selection rules ... rate of 76 MHz and has a pulse width of about 70 ps 90°-scattering geometry was employed The Raman scattered signal was collected and analyzed by a standard computer-controlled Raman system which ... of vibration of the M13 phage in solution and qualitatively and semi-quantitatively reproduced the observed Raman spectrum This indicates that Raman spectroscopy is a useful, non-destructive

Ngày tải lên: 20/06/2014, 02:20

... tape,42,43 gasoline,58 hair dye,46 soil,49 pen ink,51 and tire rubber.59 19 CHAPTER RAMAN SPECTROSCOPY 2.1 Review of Raman Spectroscopy Raman spectroscopy is a vibrational spectroscopic technique ... Discriminant Analysis 15 CHAPTER RAMAN SPECTROSCOPY 19 2.1 Review of Raman Spectroscopy 19 2.2 Blue Toners 21 2.2.1 Materials and Methods 21 2.2.1.1 Instumental ... called the Raman effect This shift provides information about vibrational, rotational and other low frequency transitions in molecules Raman spectroscopy can be used to study solid, liquid and gaseous...

Ngày tải lên: 24/08/2014, 11:52

... and guidance and my committee members: Dr Melissa Kacena and Dr Robert Yost I would also like to thank Dr Kacena and her lab for their help with the osteoclast cell culture, Dr Keith Condon and ... mice have two loxp sequences and produces a 520bp band STAT3+/+ mice lack the loxp sequences and therefore produce a smaller490bp band Cre mice produce a 615bp band if it is present (Figure 7) ... chromosome and transcription of CTSK is activated by RANKL and M-CSF Transforming growth factor β1 (TGFβ1) and interleukin 10 (IL-10) both inhibit CTSK, which has a molecular mass of 24 kDa and consists...

Ngày tải lên: 24/08/2014, 12:40

... IR and Raman spectroscopy in various scientific disciplines ranging from chemistry and physics to bioscience and medicine On the other hand, handling, manipulating and evaluating of IR and Raman ... at ~L ˆ 9394 cm–1 and the bands at wavenumbers lower and higher than n 9394 cm–1 arise from Stokes and anti-Stokes Raman scattering, respectively On the other hand, a standard Raman spectrum comprises ... version provides four different workspaces The workspaces NIR.ows and RAMAN. ows are particularly devoted to NIR spectroscopy and Raman spectroscopy, respectively All functions of OPUS are available...

Ngày tải lên: 17/03/2014, 14:46

... the book focuses on the analysis and An Introduction to Confocal Imaging presentation of confocal images The field of confocal microscopy is now extremely large, and it would be impossible to include ... of confocal, two-photon, and four-dimensional imaging Lists meetings and workshops, and a booking form for reserving time on the instruments in Madison 6.1.2 Confocal Microsxope Companies www .microscopy. bio-rad.com ... courses and CSH Press publications msa .microscopy. com Web site of the Microscopy Society of America contains useful links to other sites and microscopy societies Details of their annual conference and...

Ngày tải lên: 11/04/2014, 01:50

... Overview of Raman spectroscopy 1.3.1 The Raman effect 1.3.2 Raman instrumentation 1.3.2.1 Confocal Raman microscopy 1.3.2.2 Fiber-optic Raman spectroscopy ... vivo confocal Raman diagnostic modality was superior to NIR autofluorescence (AF) spectroscopy, but marginally higher than the co-contributions of NIR AF and confocal Raman spectroscopy The confocal ... 0.88, 0.86 and 0.56 for the confocal Raman, the composite NIR AF /Raman, and NIR AF spectroscopy modalities, respectively 80 Figure 5.1 (a) Comparison of mean in vivo Raman spectra ± standard...

Ngày tải lên: 10/09/2015, 09:24

... that CARS microscopy allowed chemically specific and label-free imaging in living organism Le et al combined CARS microscopy with TPEF microscopy and Raman spectroscopy to visualize and analyze ... appreciation and respect to Professor Dietmar W Hutmacher and Professor Hanry Yu and their group members, who taught me useful knowledge on biology and biomedicine research and offered me cellular and ... emission for easy filtering and detection In the previous work of M-CARS spectroscopy, a narrow band picosecond dye laser and a broadband pulsed dye laser were used as the pump and Stoke beam, respectively...

Ngày tải lên: 11/09/2015, 10:00