cloning expression and purification of idps in the pfs 2

... single and multiple doses of baicalin To expand on the in vivo results, the effects of baicalin on the activity and expression of CYP3A in the rat liver were examined in vitro and ex vivo. 2 ... doses of baicalin inhibited the expression of CYP3A2 in rat liver 3.3 Inhibitory Effects of Baicalin on MDZ-Hydroxylation Activity in RLMs Interestingly, the inhibition of baicalin on the appearance ... of Biotechnology, China) on X-ray films The band was then scanned, and the intensity was quantified using the Image J software (NIH) 2.7 Determination of𝐾𝑖of Baicalin against MDZ Metabolism in

Ngày tải lên: 02/11/2022, 11:45

... dịch nuôi cấy thu được 50 mg protein LLOE247M/D320K tinh sạch Hình 6 Kết quả SDS-PAGE phân tích quá trình tinh chế protein LLO E247M/D320K (A): Tinh ch ế protein dung hợp LysSRN-6xHis-TEVsite-LLO; ... pHT322 và pHT362 để tạo thành plasmid pHT363 có chứa gen mã hóa cho protein LLO được thay thế amino acid glutamate (E) ở vị trí 247 thành methionine (M) và aspatate (D) ở vị trí 320 thành lysine ... dạng LLO E247M/D320K Hoạt tính tán huyết của hai dạng protein LLO wt và LLO E247M/D320K Kết quả 1 HU của protein LLOwt và LLOE247M/D320K tương đương với lượng độc tố lần lượt 3,75 ng và 12 ng Như

Ngày tải lên: 18/02/2023, 06:47

... group of lysine residues or other primary amine The reaction leads to the formation of an isopeptide bond between two proteins and the covalent incorporation of polyamine into proteins [1,2] In ... localized at the cell surface and to mediate the interaction of integrin with fibronectin [22,23] The physio-logical significance of these multifunctional roles of TGase 2 is currently under investigation ... significantly conserved The sequences around the GTP-binding region,catalytic site,and Ca2+-binding region are highly homologous to the corresponding regions of the human TGase 2 and the other invertebrate

Ngày tải lên: 17/03/2014, 23:20

... potatoes on the Red River Delta 4 2.2.3.2 To identifying the training needs of FCRI staff and personnel in co-operatives collaborating with the FCRI in postharvest technology of seed and ware potatoes ... placed on the project by the Vietnamese counterparts as the results of the trials and training became evident 3.5 Monitoring of project In the initial design of the project, monitoring was to ... farmers and FCRI staff understanding of the dynamics of both domestic and export markets and the various means farmers have of collectively manipulating the supply of potatoes (and prices) to the

Ngày tải lên: 22/06/2014, 13:20

... of seats 1, 2 and 4, then the relative ordering is p1 < p2 ≤ q1 < p3 ≤ q2 < q3, the safe permutation is (312) and the attainable permutations are (312), (132), (213), and (123). (Throughout ... stack, then we move the top out of the stack and add it as the last element of the left list The algorithm is successful if the list L 0 contains the elements of L sorted increasingly; if this is the ... u(k)], and we say that u is stack-sortable if L u is stack-sortable For example, the sorting processes for (312) and for (231) go as follows: 312 3 12 3 1 2 1 3 3 12 3 123 2 231 2 31 2 31 2 3 1

Ngày tải lên: 07/08/2014, 07:21

... in the leaf cuticle of P halepensis Mill after a 2 min immersion of the foliage in a saline solution of LABS simulating a storm According to Schreiber and Schönherr (1993), the plant leaves in ... Sigoillot, 1982) at a concentration of 1.7 x 10mol kg H O at 22 °C Three batches of ten pines were immersed during 2 min in distilled water alone (batch 1), in LABS-distilled water (batch 2) and in LABS-sea ... not re-tained by the epicuticular wax The fraction of LABS found in the chloroform extract re-sults from the sorption and diffusion of molecules into epicuticular waxes and a part of the LABS

Ngày tải lên: 08/08/2014, 18:21

... brandy-making indus-tries that the flavour imparted by oak casks varies according to the geographic origins of the oak wood used in their construction Although the role of the hydrolyzable tan-nins in ... described in earlier studies (Scalbert et al, 1988; Viriot et al, 1994) allowed the identification of nine of these 12 ellagitannins (fig 1) Purified samples of vescalagin, castalagin, grandinin and ... roburin D shows a similar pattern to vescalagin, which contrasts with the vari-ation of roburins A and C Roburin A in-creases in concentration during the first 10 years of ageing and roburin C

Ngày tải lên: 08/08/2014, 18:21

... original work is properly cited. Trang 2the treatment of acute mania, the approval of quetiapineand the olanzapine-fluoxetine combination against acute bipolar depression and the approval of olanzapine, ... 39 in the aripiprazole group (41%) and 13 out of the 27 in the placebo group (48.1%)) The only dif-ference concerned manic relapses (nine in the placebo and six in the aripiprazole group) Again ... aripiprazole and 95 receiving haloperidol completed the first 3 weeks of treat-ment; the week 12 numbers were 89 and 50, respectively The response rate was 49.7% in the aripiprazole group and 28.4% in the

Ngày tải lên: 08/08/2014, 23:21

... Figure 2b), suggesting that CCL18 gene expression in SF PMN occurs as a result of the recruitment of PMN into the inflammatory milieu of the joint Differential expression of chemokine receptors in ... protein after 24 or 48 hours of incubation (data not shown), indicating that TNF-α induced CCL18 mRNA expression in the absence of protein synthesis Interestingly, as shown in Figure 4a, neither ... upregulation of two selected chemokine receptors, CCRL2 and CXCR4, in SF PMN, thereby confirm-ing the results of the microarray analysis These data therefore indicate that chemokine responsiveness of SF

Ngày tải lên: 09/08/2014, 10:21

... examined further the intracellular localization of the gE protein using the rabbit polyclonal antiserum specific to it in DPV-infected cells We examined the expression of gE protein in DPV-infected ... Tyrosine-containing sorting motifs in regulating the intracellular traffic of membrane proteins [33,35] The Tyrosine-containing sorting motifs usually consist of a tetrapeptide bearing the sequence ... in E coli BL21(DE3); Lane 5, the pET32a/DPV-gE after inin-duction; Lane 6, the pET-32a(+) after induction; Lane 7, the pET32a/DPV-gE be-fore induction in E coli BL21(pLysS); Lane 8, the pET32a/DPV-gE

Ngày tải lên: 12/08/2014, 04:20

... carti-lage and cathepsin K in the subchondral bone, which is in line with the findings of our previous study [6] and supports this hypothesis The anti-inflammatory effects of TLN are interesting, as they ... reduced lameness and gait dis-ability, and improved joint pain perception These find-ings are in line with the report on a rat model of OA [12] showing that the analgesic effect of the nitrogenous ... pain and functional disability in human OA [40] as well as in the canine ACL model of OA [41] The high level of synovial fluid NOx found in this study is in line with our previous findings in

Ngày tải lên: 12/08/2014, 17:21

... established in resting memory CD4+ T-cells in vitro following incubation with the chemokines CCL19 and CCL21 (ligands for CCR7), CXCL9 and CXCL10 (ligands for CXCR3) and CCL20 (ligand for CCR6) [11, 12] ... block in initiation and completion of HIV transcription as well as a block in translation of viral proteins by the expression of microRNAs (reviewed in [21]] In addition, a clear block in export of ... prostratin [24, 25], histone deacetylase inhibitors (HDACi) such as vorinostat [26, 27], methylation inhibitors [28, 29] or a combination of these approaches [25] Ideally, reactivation of virus

Ngày tải lên: 13/08/2014, 01:21

... in 2010, and then again back to finance tighten stage, restructure the economy in 2011, which lasts until the middle November 2011 and the beginning of 2012, when the economy struggles and interest ... 2.1 Introduction of BIDV 21 Fields of operation 21 History of establishment and development 22 Organizational structure 22 2.2 Analysis of BIDV business environment 24 2.2.1 Analysis of ... and budget processing and other business and commercial banking activities; Trang 22 Retailing banking activities: provide and the develop products and services of capital raising, lending and

Ngày tải lên: 26/03/2015, 10:58

... 323’UTR Over-expression of miR-221 and miR-222 in both types of tumors down-regulated p27kip1 protein expression and induced a G1 to S shift in the cell cycle, consistent with the role of p27kip1 ... involved in the control of developmental timing in C elegans uncovered a ~22-nucleotide single-stranded non-coding RNA as the product of the lin-4 gene Lin-4 RNA repressed the protein levels of lin-14, ... Argonaute proteins contain two RNA-binding domains: the Piwi domain, which binds the small RNA guide at its 5’ end, and the PAZ domain, which binds the single-stranded 3’ end of small RNA The endonuclease

Ngày tải lên: 05/10/2015, 22:31

... 323’UTR Over-expression of miR-221 and miR-222 in both types of tumors down-regulated p27kip1 protein expression and induced a G1 to S shift in the cell cycle, consistent with the role of p27kip1 ... involved in the control of developmental timing in C elegans uncovered a ~22-nucleotide single-stranded non-coding RNA as the product of the lin-4 gene Lin-4 RNA repressed the protein levels of lin-14, ... Argonaute proteins contain two RNA-binding domains: the Piwi domain, which binds the small RNA guide at its 5’ end, and the PAZ domain, which binds the single-stranded 3’ end of small RNA The endonuclease

Ngày tải lên: 06/10/2015, 20:35

... including the HAM-A psychic and somatic factors scores, the anxiety and tension items (1 and 2) of the HAM-A, and the CGI-I, in addition to the HADS anxiety and depression subscales Pregabalin ... guidelines and the Declaration of Helsinki appli-cable at the time of the study For all sites, the respective ethics committees granted approval of the protocol, and after an explanation of the ... double-blind taper and follow-up phase Efficacy Analyses The primary efficacy measure was the change from baseline to endpoint in the total score of the 14-item, clinician-rated HAM-A in the pregabalin

Ngày tải lên: 21/08/2016, 18:51

... STRATEGY OF BANK OF INVESTMENT AND DEVELOPMENT OF VIETNAM IN THE PERIOD OF 2010-2015INTRODUCTORY 1 Rational of the study Business strategy is the foundation of successful business But there are, of ... strategy in most of the popular business literature and in the thinking of most practicing executives 1.2.7 Strategic Analysis and Choice For single-or dominant-product businesses: Building sustainable ... within the business to that value.VCA takes a process point of view: It divided the business into sets of activities thatoccur within the business, starting with the inputs a firm receives and finishing

Ngày tải lên: 31/10/2016, 11:39

... Biochem 27 0) Fig Overexpression of HRT1 and HRT2 in E coli and purification of HRT2 The pETHRT1 and pETHRT2 were constructed and introduced into E coli BL21(DE3) The expression of these genes were induced ... cis-prenyltransferase, such as AAM 928 80 (AAM 928 89, AAM 928 90), AAM 928 81, AAM 928 79, BAB 920 23 (AAM 928 83, AAM 928 84, AAM 928 85, AAM 928 87, AAM 928 88), BAB 920 24 and AAM 928 82 (AAM 928 86) (submitted to GenbankTM and DDBJ by Coldren ... On the other hand, addition of WBP to the homogenate of BL21(DE3)/pETHRT2 resulted in a remarkable increase in the amount of IPP incorporation in the toluene/hexane extracts (Table 1) The increase...

Ngày tải lên: 07/03/2014, 21:20

... crab Glucose (ug/mL hemolymph) A 300 ** 20 0 100 22 to 22 22 to 29 22 to Temperature (°C) Lactate (ug/ mL hemolymph) B 180 *** 120 ** 60 * 22 to 22 22 to 29 22 to Temperature (°C) 1e+9 1e+8 b 1e+7 ... surface of the PO and many nerve fiber tracts and varicosities in trunks of the PO Cloning and sequencing of PO-CHH cDNA The first PCR of PO cDNA with a combination of a set of primers LF1 and LR1 (Table ... revealing the presence of a long 3¢-UTR ( 1.5 kbp) The amplicon (1 32 bp) produced from the nested PCR of 5¢-RACE cDNA contained a 5¢-UTR and the remaining sequence of the 5¢-end of PO-CHH The...

Ngày tải lên: 16/03/2014, 06:20

... cutting a strip of the paper into 0.5 cm pieces, from the origin to the solvent front, and counting each strip in a scintillation counter RESULTS Cloning and expression of M tuberculosis TPS The ... expressed as in Fig The insert shows the Lineweaver–Burk plot of the data authentic trehalose in solvent B and was a nonreducing sugar based on its lack of reactivity in the reducing sugar test and its ... TPS The effect of substrate concentration on the activity of the TPS was determined as shown in Figs and Fig shows the effect of increasing concentrations of either UDP– glucose or GDP–glucose in...

Ngày tải lên: 17/03/2014, 10:20