cloning and expression of a novel chemotherapeutic target
CLONING AND EXPRESSION OF a MURE LIGASE ENZYME AS a POTENTIAL TARGET AGAINST BACTERIA XANTHOMONAS ORYZAE PV ORYZAE
... Trang 1CLONING AND EXPRESSION OF A MURE LIGASE ENZYME AS A POTENTIAL TARGET AGAINST BACTERIA XANTHOMONAS ORYZAE PV ORYZAE THI-HUYEN TRAN1, NGOC-TUAN NGUYEN1, LIN-WOO KANG2 1Institute of Biotechnology ... site of action of the clinically important ß-lactam and glycopeptide Trang 2classes of antibiotics [5] Each of the disaccharide-pentapeptide peptidoglycan structure is a specific and essential ... (2µl and 8µl) IV DISCUSSION Nowadays, as appearance of multiple bacterial strains resistant to currently available antibiotics may be the main reasons to distribute the spread of resistance
Ngày tải lên: 25/10/2022, 11:33
Constitutive expression of a novel antimicrobial protein, hcm1, confers resistance to both verticillium and fusarium wilts in cotton
... at P ≤ 0.05 according to a randomization one-way ANOVA test Trang 6at 8 hours (hr), while in H213 leaves, H2O2 content was increased dramatically and appeared in two peaks at 1 hr and 5 hr (Fig. 4b) ... cecropin A-melittin, also effectively confer plants with a resistance to a broad spectrum of patho-gens33,34,35 Resistance to tobacco mosaic virus, bacterial Ralstonia solanacearum, and fungal Magnaporthe ... H177, and H213) and parent W0 plants Error bars represent the standard deviation of triplicate experiments, and the EF-1α gene was amplified as a control (e) Western blot analysis of Hcm1 in transgenic
Ngày tải lên: 19/11/2022, 11:47
Báo cáo khoa học: "Identification and characterisation of a novel anti-viral peptide against avian influenza virus H9N" pps
... 5'ATTTAAGGATCCGAGAGCCATGGA 3' 5'ATGCTGCTCGAGTTATATACAAATGTTGC 3' 5'CATAGAATTCGCAAAAGCAGGAGT 3' 5'TATCGCTCGAGAGTAGAAACAAGGAG 3' 5'AGCCTGGAATTCATGAAAAAATTA 3' 5'CTCACTCGAGACATTTTCAGGGA 3' aIn all of the above mentioned ... AGCCTGGAATTCATGAAAAAATTA 3' 5' ATCGAACTCGAGATTTTCAGGGAT 3' 5' AGGGCTGGCGGTTGGGGGTTATTCGC 3' 5' GAGTCACTTTAAAATTTGTATACAC 3' 5' GATGTTAACGATACCAGCC 3' 5' GCGTGAATGTAAGCGTGAC 3' 5'ATTTAAGGATCCGAGAGCCATGGA 3' ... pC-HA-F pC-HA-R pC-NA-F pC-NA-R pC-P1-FP pC-P1-RP 5' CATAGAATTCGCAAAAGCAGGAGT 3' 5' TATCGCTCGAGAGTAGAAACAAGGAG 3' 5' ATTTAAGGTACCGACAGCCATGGA 3' 5' ATGCTGCTCGAGTATACAAATGTTGC 3' 5' AGCCTGGAATTCATGAAAAAATTA
Ngày tải lên: 12/08/2014, 04:21
Development and evaluation of a novel nanoparticulate delivery system of arsenic sulfides
... with anthracycline-based chemotherapy Therefore, Trang 28ATO offers advantages over ATRA with anthracycline-based chemotherapy in the treatment of relapsed or refractory APL, and becomes a standard ... of apoptosis through depolarization of mitochondrial membrane, activation of caspase-9, caspase-3 and PARP [Akao Y et al., 1999] Trang 30 Engaging in the extrinsic pathway by up-regulation of ... realgar (As2S2) was used together with an equal amount of ground Seman Platycladi as an excipient to make capsule containing 250 mg realgar Realgar was orally administered at a dosage of 50
Ngày tải lên: 11/09/2015, 16:03
Identifcation and stablization of a novel 3d hepatocyte monolayer for hepatocyte based applications
... 3-methylcholanthrene AHG 1-O-(6’-aminohexyl)-D-galactopyranoside AMC Academic Medical Center ALF Acute liver failure ALT Alanine Transaminase APAP Acetaminophen ASGPR Asialoglycoprotein Receptor BC Bile Canaliculi ... BLAD Bio-artificial liver assisted... and are separated from plasma by the capillary membranes Before entering the bioreactor, the plasma passes a charcoal absorber for detoxification and ... chemicals and in the mechanistic evaluation of toxicological phenomena A large amount of natural and synthetic chemicals are hepatotoxins In many cases, the toxicity is metabolism-mediated
Ngày tải lên: 12/09/2015, 08:19
Identification and characterization of a novel heart reactive autoantibody in systemic lupus erythematosus possible serological marker for early myocardial dysfunction
... non-white population of the USA, Europe, Canada and Australia (Danchenko et al 2006a) Trang 281.1.2 Pathogenesis of SLE The pathogenesis of SLE relates to abnormalities of both the innate and adaptive ... prevalence and incidence in this population than in Australian Caucasians Both racial and genetic differences, and more complex social factors related to poverty and access to care are likely associated ... anticholesterol antibody aCL anticardiolipin antibodies ANA antinuclear autoantibody ANP atrial natriuretic peptide APS anti-phospholipid syndrome aPL anti-phospholipids antibodies ARA American Rheumatology
Ngày tải lên: 14/09/2015, 12:42
Roles of siRNAs and miRNAs in host responses to virus infection identification and characterization of a novel viral suppressor of RNA silencing
... method against human viral and cancer diseases (Aoki et al., 2003; Coburn and Cullen, 2002; Park et al., 2002b; Yamamoto et al., 2002) Trang 26of the target DNA as well as degradation of the target ... demonstrated as a natural antiviral defense in plants Is RNAi also antiviral in animals? Flock house virus (FHV), which can infect both animals and plants, was demonstrated as both an initiator and a ... regulate their target mRNAs 1.3.6 Interaction between viral suppressor and miRNA regulation Trang 41.5 Rationality and Aims of the project Chapter 2 General materials and methods 2.1 Plant materials
Ngày tải lên: 16/09/2015, 17:11
Cloning and expression of the plasmodium falciparum metacaspase gene PfMCA1
... GTACAACCATTCAATTCATATTTGG PfMCA-Pact-3’-4300 AAGAAACTTCCTTATCTTTACATCCAC PfMCA-Pact-3’-4700 AGGGTGGTTTAAAAATAGAAATAGAG PfMCA-pESC-rv-5202 GATTGGAGTTATGTAAATCATTAGATGC PfMCA-pESC-rv-5601 GACCAGAAAATAGGAAGAACAGAATG ... PfMCA-Pact-5’-2295 CCTCACCCTAACATATTTTCCAATTAAC PfMCA-Pact-5’-2700 CTTACTGCTTTTTTCTTCCCAAG PfMCA-Pact-5’-3100 ATTGATGTTGTAAAGAAATGTACATTGC PfMCA-Pact-5’-3500 ATAGCACTTATATGAACAATTCACCTAC PfMCA-Pact-3’-3800 ... (GCCGAATTCATGGAAAAAATATACGTCAAAAT) and 3’PfMCA-SalI (GGGCGTCGACTAAAAAAAAAATAAATTTTTAAGTTC), with the EcoRI and SalI restriction sites underlined respectively Subsequently, the reverse primer was modified
Ngày tải lên: 03/10/2015, 20:57
Isolation, cloning and expression of NAD+ glycohydrolase from neurospora crassa
... NAD+ Metabolism and NAD+ Glycohydrolase 1.1.1 Mammalian NADase 1.1.2 Neurospora crassa NADase 1.2 cADP-Ribose and Ca2+ Signalling 11 1.3 N crassa NADase in Cycling Assay 13 1.4 Neurospora crassa ... 664-666 Kato I Takasawa S., Akabane A., Tanaka O., Abe H., Takamura T Suzuki Y., Nata K., Yonekura H., Yoshimoto T and Okamoto H 1995 Regulatory role of CD38 (ADP-ribosyl cyclase/cyclic ADP-ribose ... LIST OF FIGURES FIGURE TITLE PAGE 1.1 De novo synthesis and salvage pathway of NAD+ 1.2 Reaction mechanism of NADase and ADP-ribosyl cyclase 1.3 Cycling assay for cADPR 15 1.4 Asexual cycle of
Ngày tải lên: 08/11/2015, 17:15
Simulation and analysis of a novel micro-beam type of mems strain sensors
... the values of elastic limit of Si material in wide range of conditions Thus, the MEMS strain gauge can be operated safely to avoid the break of strain gauge in wide range of the tension load and ... direction of the stress and strain on the material objects 2 MATERIALS AND METHODS 2.1 Theoretical background for mechanical analysis system In this theoretical analysis, the strain gauge problem can ... strain sensors Trang 9Table 2 Summary of longitudinal and transverse strain (ε xx, εyy) of some metals and semiconductor strain sensors (εxx, εyy) Rajanna and Mohan [3] thin foil sensors metal:
Ngày tải lên: 13/01/2020, 12:21
Design and evaluation of a novel felbinac transdermal patch: Combining ion-pair and chemical enhancer strategy
... 2000;207:31 –7. 4 Shiba T, Shikata Y, Takagawa N, Sasaki M, Yanaka M, Sugiyama Y, et al Antiinflammatory and analgesic activities of felbinac adhesive preparation (felbinac patch) Jpn Pharmacol Ther 1992;20:81 ... was used as the internal standard and detection wavelength was set at 254 nm Data Analysis Each experimental value was an average of minimum four measurements Statistical analysis was conducted ... through ear marginal vein of rabbit (n=4) b Plasma concentration-time profiles of FEL after transdermal administration of FEL-TEA patch and commercial FEL patch at the abdominal site of rabbit (n=4)
Ngày tải lên: 11/05/2020, 11:58
Molecular cloning and characterization of a flavonoid-O-methyltransferase with broad substrate specificity and regioselectivity from Citrus depressa
... program Arabidopsis thaliana (At3OMT, GenBank accession number AED96460 and AtOMT1, AAB9679), Catharanthus roseus (CrFOMT, AAM97497 and CrOMT6, AAR02419), Chrysosplenium americanum (Ca3 ′OMT1, AAA80579), ... producing various PMFs Methods Strains and vectors Fruits of C depressa Hayata (known as shequasar or Taiwan tangerine) were purchased from an agricultural cooperative in Okinawa, Japan to obtain genomic ... number ABP94018), Arabidopsis thaliana (At3OMT, AED96460), Chrysosplenium americanum (Ca3 ′OMT1, AAA80579), Mentha × piperita (Mp3 ′OMT, AAR09601), and Triticum aestivum (Ta3′4′5′OMT, ABB03907) Amino
Ngày tải lên: 22/05/2020, 04:36
Cloning and expression of recombinant VP2 capsid protein gene of canine parvovirus in E. coli System
... A-72 (Kumar et al., 2003; Rai et al., 2004 and Hirayama et al., 2005) Many diagnostic tests lack specificity and sensitivity whereas many are very laborious and time consuming ELISA assays have ... Mukhopadhyay1, Mouttou Vivek Srinivas1*, Muthuraj Muthaiah3, Prabhakar Xavier Antony1 and Jacob Thanislass2 Department of Veterinary Microbiology, 2Department of Veterinary Biochemistry, Rajiv Gandhi ... detection of CPV from faecal samples of dogs like haemagglutination test (Carmichael and Binn et al., 1981), latex agglutination test (Veijalainen et al., 1986), immunochromatographic test (IC) (Esfandiari
Ngày tải lên: 17/06/2020, 13:53
Development and validation of a novel pseudogene pair-based prognostic signature for prediction of overall survival in patients with hepatocellular carcinoma
... regression analysis (univariate and least absolute shrinkage and selection operator) All individuals were classified into high- and low-risk groups based on the optimal cutoff Subgroups analysis of the ... develop and validate an accurate and robust pseudogene pairs-based signature for the prognosis of hepatocellular carcinoma (HCC) Methods: RNA-sequencing data from 374 HCC patients with clinical follow-up ... University, No.277, Yanta West Road, Xi ’an 710061, Shaanxi, People’s Republic of China Full list of author information is available at the end of the article Trang 2Hepatocellular carcinoma (HCC) is the
Ngày tải lên: 28/09/2020, 09:50
Design, fabrication and control of a novel linear magnetic actuator for a controllable squeeze film damper
... and applied in a wide range of the industrial actuators, such as LMAs, tubular permanent-magnet generator/machines, linear oscillo-actuators and generators have been increasingly researched and ... which applied appropriately to the Controllable Squeeze Film Damper The novel configurations of LMA and SFD were proposed, designed and fabricated All of the evaluated experimental apparatus and ... Trang 1 , Design, Fabrication and Control of a Novel Linear Magnetic Actuator for a Controllable Squeeze Film Damper Truong Quoc Thanh Trang 2 , Design, Fabrication and Control of a Novel
Ngày tải lên: 31/01/2021, 23:18
Báo cáo khoa học: cDNA cloning and characterization of a novel calmodulinlike protein from pearl oyster Pinctada fucata potx
... nucleotide sequence of oyster CaLP cDNA obtained by RACE, a PCR reaction was performed using a pair of specific primers P3 (5¢-GGAAGAATACAGACACGGACAG-3¢) and P4 (5¢-ATAACAACAGTTTATACATCGCTTC-3¢) corresponding ... sequence of CaLP2 were used in the nested PCR reactions The first round of PCR reaction was performed with a forward primer UPM (a mixture of primers 5¢-CTAATACGACTCACTATAGGGC AAGCAGTGGTAACAACGCAGAGT-3¢ ... sequence, an open reading frame consisting of 483 bp, a TGA stop, a 146 bp 3¢-untranslated sequence, and a poly (A) tail of 18 nucleotides A putative polyadenylation signals (AATAAA) is recognized at...
Ngày tải lên: 16/03/2014, 23:20
Cloning and characterization of a novel kelch like gene in zebrafish
... great potential to serve as a model for human disease that range from heart failure and vascular disease to fields as diverse as osteoporosis, renal failure, Parkinson’s disease, diabetes and cancer ... Postlethwait and Talbot, 1997) The two main approaches of cloning mutated genes, positional cloning and candidate gene approach, have benefited greatly from the recent advances in zebrafish genomic ... et al., 2000) Similar results were obtained in another study done at the same time (Barbazuk et al., 2000) Analyses of mouse and human, as well as zebrafish and human synteny groups have also...
Ngày tải lên: 03/10/2015, 20:57
Báo cáo khoa học: Pulchellin, a highly toxic type 2 ribosome-inactivating protein from Abrus pulchellus Cloning, heterologous expression of A-chain and structural studies ppt
... human and rabbit erythrocytes, and kills mice and the microcrustacean Artemia salina at very low concentrations [21] Here we report the cloning of pulchellin A- chain (PAC), its cDNA characterization, ... based on DNA sequences obtained previously Thus, the sequences of the primers used for 5Â RACE were: 5Â-GGGCATCACGGA AGAAATAG-3Â for a reverse transcription and 5Â-GC TCTAGAGCATTCGTCACATCGATACC-3Â ... enzymatic activity of rPAC Figure shows an Fig Deduced amino acid sequence of recombinant pulchellin A- chain (rPAC) aligned to abrin -a, abrin-c and ricin (RTA) A- chains Conserved amino acids are highlighted...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: Cloning, characterization and localization of a novel basic peroxidase gene from Catharanthus roseus potx
... AY032675 DQ650638 AY206412 AY206413 AF244923 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA At5g40150 NA NA NA NA NA NA NA NA NA NA NA NA At5g05340 NA NA NA NA NA NA NA NA NA Unpublished Unpublished ... Primer pairs were: #GSP-4 and #PFLF1 (lanes and 2); #GSP-2 and #GSP-4 (lanes and 4); #GSP-2 and #PFLR-1 (lanes and 6); and #PFLF-1 and #PFLR-1 (lanes and 8) (B) Schematic organization of the CrPrx ... database, i.e Avicennia (BAB16317), Nicotiana secretory peroxidases (AAD33072), cotton (COTPROXDS) (AAA99868), barley grain (BP1) (AAA32973), Ar thaliana (ATP 2A) A2 (Q42578) and HRP-C (AAA33377) Residue...
Ngày tải lên: 23/03/2014, 09:21
TARGETING ACUTE PHOSPHATASE PTEN INHIBITION AND INVESTIGATION OF A NOVEL COMBINATION TREATMENT WITH SCHWANN CELL TRANSPLANTATION TO PROMOTE SPINAL CORD INJURY REPAIR IN RATS
... using a general rat endothelial cell marker, and calculation of the vessel-positive area was performed in the ipsilateral and contralateral gray matter in vehicle- and bpV-treated animal groups ... the dynamics of cervical SCI and related intracellular signaling and death mechanisms is essential Through behavior, Western blot, and histological analyses, alterations in phosphatase and tensin ... (Unoki and Nakamura, 2001) It is widely known that activity of the mammalian target of rapamycin (mTOR) is a major inhibitor of the progression of apoptosis, and stability of mitochondrial function...
Ngày tải lên: 24/08/2014, 09:14