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... carried the wild-type F0 operon on the plasmid and the deletion of the chromosomal F0 operon The cells used for chromatophores preparations were routinely checked for the presence of the mutation ... in the case of the mutant The large difference relative to the wild-type can be best appreciated by taking the derivative of the fitting functions of the mutant and wild-type data and plotting their ... plotting the ratio of the fitting functions of wild-type over mutant, either in the presence or in the absence of a Du, as shown in Fig 3C In the presence of a Du, the difference between the two...

Ngày tải lên: 21/02/2014, 03:20

Ngày tải lên: 14/03/2014, 20:20

... section of the bar The size of this section is dependent on the size of the resource being returned and the available bandwidth The number following the bar is the total time for the resource, ... rst line indicates the point at which the browser began to render the page The second line indicates the point at which the onLoad event fi red — that is, the point at which the page had fi nished ... on other hardware Usually, this is the disks, so check for high values in the io and swap columns Whether the CPU will mostly be running user code or kernel code depends on the nature of the...

Ngày tải lên: 17/03/2014, 12:20

... [28] Furthermore, though these substitutions modified the interaction of the reduced enzyme with the IP product, as indicated by the observation that Y228F totally abolished the formation of the absorbance ... of EFlseq to the oxidized and reduced forms, with the endpoint containing the thermodynamically stabilized amount of semiquinone The Y238S mutant showed a higher percentage of the thermodynamically ... [17], in order to assess changes in the thermodynamic properties of the flavin centre caused by the mutation and to explain the different thermodynamic stability of the semiquinone form with respect...

Ngày tải lên: 17/03/2014, 17:20

... pH < 6.5 the signal was absent The profile of the entire signal, without interference of the rhombic signal, was obtained by subtracting the pH 6.4spectrum from the pH 8.4-spectrum (see the inset ... (a) The signal profile and the position of g values fundamentally deviate from that of the P1+ signal (g ¼ 2.06, 1.96, 1.83) of the Rhodobacter MoFe protein Furthermore, the redox potential of the ... characterized These are the enzymes of Azotobacter vinelandii [5,6], Rhodospirillum rubrum [9] and Rhodobacter capsulatus [8,18,19], the heterometal-free N2-fixation system from the latter organism being the...

Ngày tải lên: 18/03/2014, 01:20

... relationship Of course, the quality of the relationship matters too The deeper the relationship, the greater the odds that we will listen to someone, be persuaded by them, or take action at their request ... failing to recognize the gap between what they know about the organization and what the board and other close friends of the organization know The Executive Director lives and breathes this stuff ... The organization runs like a rat around the wheel, but never gets the wheel to the next level In my experience, these organizations fail to hit the next level because they fail to recognize the...

Ngày tải lên: 18/03/2014, 03:20

... State of the World’s Mothers report The focus is on the critical shortage of health workers in the developing world and the urgent need for more female health workers to save the lives of mothers, ... alongside other female health providers in the community have the greatest potential to improve the reproductive health of women and save the lives of mothers and babies in the developing world The ... in This Report The State of the World’s Mothers Report uses the most up-to-date information available to describe the health of mothers, newborns and children around the world The data used in...

Ngày tải lên: 22/03/2014, 15:20

... lack of the opsinobligatory interaction K296 (7.43) in the latter In the context of the crystal structures (Fig 5), the G51V mutation changes the environment of D83, thereby modifying the interactions ... II [22] The G51A, G51V and G51L mutant proteins were thermally less stable compared with WT rhodopsin, both in the dark and after photoactivation Both the stability of the mutants and their ability ... correlated with the increase in size of the side-chain at position 51, pointing to a disruption of the interhelical packing due to the mutations In the case of mutations at position 89, the charge...

Ngày tải lên: 22/03/2014, 16:20

... as it were, the soil; the tenets of our teacher are, as it were, the seed; instruction in youth is like the planting of the seed in the ground at the proper season; the place where the instruction ... against the wall, their features all changed, and they neither saw, heard, nor spake, and their clothes were still smouldering where the gunpowder had burned them As I was looking at them with ... all The Lord Marshal sent me now hire now there to dress the soldiers committed to me who were wounded in other cities beside Turin, so that I was always in the country, one way or the other M the...

Ngày tải lên: 22/03/2014, 23:20

... biosynthesized by various OSCs (Fig 1) and these enzymes regulate the isoprenoids pathway controlling the biosynthetic flux towards either triterpenoids or phytosterols [11] Of the members of the ... biosynthetic pathway of terpenoids in these plant species, and to further our knowledge on the physiological significance of these compounds Very recently, we were the first group to identify the ... before their evolution to product-specific OSC Therefore, the terpene synthase of B gymnorrhiza may be considered to be a more highly evolved form compared to the terpene synthase in the other two...

Ngày tải lên: 23/03/2014, 07:20

... that the fault was there when you bought it In these circumstances you can ask the dealer to repair the car free of charge or replace it Before you buy ask the salesperson these questions • Is the ... www.smmt.co.uk) The code from the SMMT, who represent all sectors of the automotive industry in the UK, says that you are free to get your car serviced anywhere in the UK and still benefit from the manufacturer’s ... the SMMT code which permits servicing by independent garages? • How much will the servicing costs be? (Compare them to other garages in your area) • How much leeway is there if I cannot get the...

Ngày tải lên: 23/03/2014, 10:20

... synthesized [20] RC protein was purified from Rb sphaeroides strain R26 [21] Either the native UQ10 was removed from the QB site or the native UQ10 was removed from the QA and QB sites [22] The ... methoxy group and the oxygen at C4 lower the binding order of the carbon C4 and strengthen the downshift of the 4C¼O mode [10,11] In principle, X-ray-based structural models of the reaction centre ... [10] The difference spectra with unlabelled and 13C-labelled UQ10 at the QA or QB sites were normalized on the 1800– 1700 cm)1 region, which was unaffected by the labelling The IR spectra of the...

Ngày tải lên: 23/03/2014, 21:20

... retrieve all the clauses and phrases in the corpus that encode a particular local rhetorical relation I then hypothesized functional reasons for alternations in form and tested them with the data ... this the terminating condition form In this case, the use of this form marks the fact that the readers don't have to redo the action PURPOSES One important factor in the choice of form is the ... preference for the use of less prominent grammatical forms (in this case, phrases rather Here, the agent that performs the action determines, in part, the form of the expression In (4a), the action...

Ngày tải lên: 31/03/2014, 06:20

... inch, the the PUSHING TO hills surrounding FRONT THE were held soon Union by soldiers Were these they were things compelled of Did the Tuscans the Tiber idas march the of of Armada? he from gathered ... the back Lightning had days of bus Columsea men on from fallen but Newton fall the head earth the by their of momentum from first in planets if as the was the to the universe the trees, all them ... office they in the while there where world only there now " was classes these the were than successes of three there one, little A four or fifty And are the to through avenues " reach of there...

Ngày tải lên: 02/04/2014, 13:57

... Now if we read the word Lord (law) it will give us the key to the statement.   The fear of the law (Karmic law) is the beginning of wisdom (not the fear of the Lord).   When we know the whatever we send out comes back, we begin to be afraid of our own  ... One day he seemed to feel his room moving. He felt the motion of a ship. He went to the window to breathe the fresh air, and it smelt like the aroma of the docks. With his inner  ear he heard the shriek of a sea‐gull and the creaking of the gangplank. The trunk had  ... hand  over  the sea,  and  the sea  returned;  and  the Egyptians fled against it, and the Lord overthrew the Egyptians in the midst of the sea."   "And the waters returned, and covered the chariots, and the horsemen, and all the hosts ...

Ngày tải lên: 02/04/2014, 13:58

... for the abandonment of the other If the costs were not approximately equal, then there would be no dilemma to start with Now, if the problem of the rhetorical presidency were derived from the ... that we attend to the substance of presidential rhetoric as we observe the expansion of the rhetorical presidency into the rhetorical executive Not only is over one-third of the contemporary White ... objections would remain of the rhetorical presidency We would then be left with the problem of the unequal rhetorical balance of power between the president and congress, but then this becomes a problem...

Ngày tải lên: 10/06/2014, 21:26

... love the whole mythology of that brand I love the fact that Kohler is the name of the brand and the town it originated in, and the head of the company is the mayor of the town The work they is ... wants them to He tells them he wants them to change the world He insists that they think bigger than they’re able to think Then, as they sit there, he tells them what he finds remarkable about them ... Our team designed the Microsoft Store logo and it gave me chills to see the logo lit above the door to the store, on the bag, and on employee uniforms The BFGoodrich pitch was the first one I ran...

Ngày tải lên: 28/06/2014, 17:20

... become available, they, too, will need to meet or exceed the safety standards set by the currently available therapies for AR Understanding the potential concerns and the data behind these concerns ... between any of the treatments and placebo Owing to the short nature of the study and the large variability inherent in knemometry, these large treatment effects did not reach the predetermined ... had normal growth prior to the study During the study, the children had their heights measured by stadiometry at months 1, 2, 4, 6, 10, and 12 of treatment They also had their HPA axis assessed...

Ngày tải lên: 08/08/2014, 21:20