characterization of nonstructural protein 5b

... isolation of potential interacting protein partners for your protein of interest The knowledge requirement for your protein of interest is only very minimal If a protein has a known function, new proteins ... infection of human herpesvirus 6 3 Table 3: Genetics of human herpesvirus 6 (HHV-6) and Trang 8LIST OF FIGURES1 2.1.1: Principle of the Two-Hybrid system 17 2 2.2.1: Cartoon representation of a ... life cycle of Picornavirus 23 3 2.2.2: Cartoon representation of the assembly and packaging of a virion 24 4 2.2.1.1:Genotypic classification of Enterovirus 71 27 6 2.2.1.3 Typical route of infection,

Ngày tải lên: 12/09/2015, 08:19

... Molecular Characterization of Coat Protein Gene of Blackgram Yellow Mosaic Virus (BGYMV) from Karnataka, India G.U Prema 1* and K.T Rangaswamy 2 1 Department of Plant Pathology, College of Agriculture, ... 880 bp with 106 bp of pre-coat protein region and 774 bp of coat protein region of blackgram yellow mosaic virus 257 amino acid lengths were predicted after translation of the nucleotide sequences ... used for amplification of coat protein gene of Blackgram yellow mosaic virus (BGYMV) Primers were designed to get the complete coat protein gene of yellow mosaic viruses of legume hosts by taking

Ngày tải lên: 21/05/2020, 21:47

... regardless of ionic strength 1.1.2.1 Myofibrillar proteins Myofibrillar proteins are made up of many different proteins, with myosin and actin being the two major ones In structural proteins, ... abundant proteins making up between 70-80% of the total proteins are the structural proteins that build up the contractile unit (sarcomere) of the myofibril (the muscle fibre) The sarcoplasmic protein ... potential of TDMPI as a function of pH 52Figure 3.12: Variation of zeta potential of TDMPI in the presence of 0.005M (a) and 0.01 M (b) salt as a function of pH 53Figure 3.13: Viscosities of different

Ngày tải lên: 25/01/2021, 23:27

... regardless of ionic strength 1.1.2.1 Myofibrillar proteins Myofibrillar proteins are made up of many different proteins, with myosin and actin being the two major ones In structural proteins, ... abundant proteins making up between 70-80% of the total proteins are the structural proteins that build up the contractile unit (sarcomere) of the myofibril (the muscle fibre) The sarcoplasmic protein ... potential of TDMPI as a function of pH 52Figure 3.12: Variation of zeta potential of TDMPI in the presence of 0.005M (a) and 0.01 M (b) salt as a function of pH 53Figure 3.13: Viscosities of different

Ngày tải lên: 17/02/2021, 21:05

... regardless of ionic strength 1.1.2.1 Myofibrillar proteins Myofibrillar proteins are made up of many different proteins, with myosin and actin being the two major ones In structural proteins, ... abundant proteins making up between 70-80% of the total proteins are the structural proteins that build up the contractile unit (sarcomere) of the myofibril (the muscle fibre) The sarcoplasmic protein ... potential of TDMPI as a function of pH 52Figure 3.12: Variation of zeta potential of TDMPI in the presence of 0.005M (a) and 0.01 M (b) salt as a function of pH 53Figure 3.13: Viscosities of different

Ngày tải lên: 17/05/2021, 14:16

... of the Fe-protein (dinitrogenase reductase, also referred to as NifH), an a2dimer of the nifH gene product and of the MoFe-protein (dinitrogenase) with an a2b2 symmetry [3] ADP-ribosylation of ... Fe-protein are marked by arrows A, Unmodified Fe-protein; B, modified Fe-protein. Fig 3 Analysis of both nitrogenase Fe-protein isoforms from conventional Coomassie stained SDS ⁄ PAGE gels by TOF ... The exchange of R102 by alanine led to a shift of the protein during SDS⁄ PAGE, which was observed previously in R capsulatus [33] Optimization of protein processing for MS analysis of the modified

Ngày tải lên: 07/03/2014, 02:20

... Oxidase and isomerase activities of cPDI measured using conotoxins as substrates. Oxidase a,b Moles of reduced tx3a per mole of oxidase Moles of nTx3.1 per mole of isomerase per min (· 10)3) a ... absence of 200 gÆL)1Ficoll 70 The amounts of nTx3.1 and sTx3.1 were calculated from their elution peak areas The data are the average of three independent experiments For the rate of increase of the ... products of Sigma (St Louis, MO, USA) Other reagents were of analytical grade Gene cloning of cPDI The full-length cDNA of cPDI was amplified by RT-PCR from total RNAs isolated from the venom ducts of

Ngày tải lên: 07/03/2014, 05:20

... 4Nucleotide sequences of the ORF of both PDI geneswere exactly the same as those of cDNAs cloned in this study Comparisons of soybean genomic sequences of GmPDIL-1 or GmPDIL-2 with those of Arabidopsis ... as an ER lumen protein, were detected at a density of 1.21 gÆmL)1 in the presence of MgCl2 In the presence of EDTA, which causes release of ribo-somes from the rough ER, the peaks of GmPDIL-1, ... with two soybean proteins of comparable masses that had similar pIs It is unclear whether either protein was the product of the GmPDIL-1 gene or of different genes As the masses of both bands appeared

Ngày tải lên: 07/03/2014, 05:20

... Comparison of the fasciclin I domains in CLAP with selected proteins in the protein database The Conserved Domain Database of NCBI was screened with the protein coding sequence of clone 1 of CLAP ... consisting of a catalytic subunit (CL) of 28.5 kDa and noncatalytic subunit of 31.5 kDa with a total molecular mass of 60 kDa [23,24] The catalytic subunit of the complex has a high degree of homology ... The number of pmoles of cathepsin L were determined by titration of the active site with E-64 as described previously [29] The concentration of heterodimeric cathepsin L was 64–65% of that calculated

Ngày tải lên: 07/03/2014, 16:20

... Analysis of T-DNA insertion mutants and RNAi mutants of A thaliana homologs of OsRPA70a and OsRPA70b (AtRPA70a and AtRPA70b) A thaliana was used for genetic analysis of the functions of OsRPA70a ... efficiency of the PCR (Fig 1B) We performed Western blot analysis of total proteins from seedlings of wild-type, atrpa70b, and the AtRPA70b RNAi mutant, and confirmed that very little AtRPA70b protein ... Replication of simian virus 40 origin-containing DNA in vitro with purified proteins Proc Natl Acad Sci USA 84, 1834– 1838 Wold MS & Kelly T (1988) Purification and characterization of replication protein

Ngày tải lên: 07/03/2014, 21:20

... Biochemistry, Faculty of Medicine, National University of Singapore, Republic of Singapore Institute of Molecular and Cell Biology, A*STAR Biomedical Research Institutes, Singapore, Republic of Singapore ... overexpression of ORP2 led to a significant reduction of ACAT activity and cholesterol esters [8] Overexpression of a splice variant of ORP4 (ORP4-S) caused a 40% reduction in esterification of low density ... osh6D or OSH6 overexpressing cells (data not shown) Characterization of the functional domains of Osh6p As a short Osh protein, Osh6p consists of a conserved ORD domain that is believed to mediate

Ngày tải lên: 07/03/2014, 21:20

... Trang 1Identification and characterization of B¢¢-subunits of proteinEvidence for an independent N-terminal splice variant of PR130 and an extended human PR48 protein Ilse Stevens1, Veerle ... Xenopus orthologue of PR130 (XPR130) A second class of clones (XN73) is identical to the N-terminal part of XPR130 but ends a few amino acids downstream of the putative splicing site of PR130 To investigate ... regulates a number of different cellular events The phos-phorylation state of a protein is dependent on the opposing activities of kinases and phosphatases Among the phos-phatases, protein phosphatase

Ngày tải lên: 08/03/2014, 08:20

... and handling of sample solutions was mini-mized, because these processes caused loss of the protein in solution Inspection of the chemical forms of two Cys residues in the recombinant protein was ... N-terminal region of cp-19ks (supplementary Fig S1B), indicating that the protein homologous to cp-19k should also be present in B eburneus cement Characterization of the recombinant Mrcp-19k protein Recombinant ... cytosolic space of several organ-isms, including bacteria and fish [17], the Ala and⁄ or methyl group of Thr on the molecular surface of the protein are known to be essential in the process of binding

Ngày tải lên: 16/03/2014, 05:20

... with the amount of GmPDIL-3a mRNA, although the amount of GmPDIL-3b mRNA was not consistent with the amount of GmPDIL-3b protein expression (Fig 5B) Expression of many ER-resident proteins can be ... transport of ER proteins, or regulation of ER calcium channel activity [9–12] In plants, a set of 22 orthologs of known PDI family proteins was discovered by a genome-wide search of Arabidopsis ... isolation of membrane protein complexes in enzymatically active form Anal Biochem 199, 223–231 Walker KW, Lyles MM & Gilbert HF (1996) Catalysis of oxidative protein folding by mutants of protein...

Ngày tải lên: 18/02/2014, 11:20

... present the mesoscopic model of biological protein materials composed of protein crystals prescribed by Go potential for characterization of elastic behavior of protein materials Specifically, ... mechanical characterization of protein materials may allow for gaining insight into the biological functions of mechanical proteins Mechanical characterization of biological molecules such as proteins ... Fig Schematic illustration of biological protein materials composed of protein crystals (a) cartoon of a fiber, made of protein crystals, under mechanical loading (b) protein crystal lattices constituting...

Ngày tải lên: 22/02/2014, 08:20

... quality factor of cantilever and phase of the cantilever relative to the driver [29] The wear rate ranges between Page of 10 Figure Wear of protein layers in tapping mode AFM AFM image of FBS layer ... employed for characterization of amplitude variations of topography and phase across the surface RMS values were also used as an error bar of determined layer thickness [8] Page of 10 Results ... previous reports [30,31] The slope of linear regression of the data is 2.1 × 10-3 scan-1 It should be noted that this is not wear of individual protein molecules but of the layer adsorbed as a whole...

Ngày tải lên: 21/06/2014, 04:20

... approaches to the derivatization of CdSe/ZnS QDs to achieve conjugation of active agonists of the A2AAR The problems of limited aqueous solubility of the QD [20-23] and access of the flexible tethered ... (20 μg of protein) , 50 μL of agonist radioligand, and 50 μL of increasing concentrations of the test ligands in Tris-HCl buffer (50 mM, pH 7.5) containing 10 mM MgCl2 The concentration of the ... conjugates of quantum dots for characterization of G protein- coupled receptors: strategies for immobilizing A2A adenosine receptor agonists Journal of Nanobiotechnology 2010, 8:11 Page 19 of 19 ...

Ngày tải lên: 11/08/2014, 00:22

... glycoprotein I, a protein localized in the transGolgi network Embo J 1996, 15:6096-6110 Alconada A, Bauer U, Sodeik B, Hoflack B: Intracellular Traffic of Herpes Simplex Virus Glycoprotein gE: Characterization ... 5, the pET-32a(+) after induction; C Effect of different time of the pET32a/DPV-gE protein in Rosseta M: Protein marker; Lane 1-6, the pET32a/DPV-gE protein was expressed respectively for 2, 3, ... detected the product of the gE gene Intracellular localization of the gE product in DPV-infected cells To confirm the intracellular localization of gE protein, indirect immunofluorescence studies...

Ngày tải lên: 12/08/2014, 04:20

... total of 11 paralogous protein families with more than one hundred member proteins were identified in rice which encoded proteins such as zinc finger proteins, protein kinases, Myb-like proteins, ... sion (r) of of paralogous protein families with exactly two HistogramricePearson's Correlation Coefficients of expresHistogram of Pearson's Correlation Coefficients of expression (r) of rice paralogous ... 171 147 109 87 78 >8 Number of Proteins in Each Family Figure Arabidopsis Size distribution of paralogous protein families in rice and Size distribution of paralogous protein families in rice and...

Ngày tải lên: 12/08/2014, 05:20

... presentations of apoCaM and Ca2+/CaM 20 Fig 1.9 Summary of CaM binding proteins 22 Fig 1.10 Comparison of the in vivo (with PTMs) and in vitro (without PTMs) states 23 Fig 1.11 A list of protein interaction ... Purification profile of Cbl-TKB 49 Fig 2.2 Gel filtration profile of cleaved Cbl-TKB 49 Fig 2.3 Glutaraldehyde cross-linking of Cbl-TKB 50 Fig 2.4 Initial crystals from the screening of Cbl-TKB complexed ... N Number of binding sites Nav Voltage gated sodium channel isoform Nav1.4IQ IQ motif of voltage gated sodium channel isoform 1.4 Nav1.6IQ IQ motif of voltage gated sodium channel isoform 1.6...

Ngày tải lên: 11/09/2015, 09:11