centralized layered and design based approaches

... as important, and the other sentences as not important We included this approach merely as a simple baseline 2.2 Word -based approaches Word -based approaches to summarization are based on the idea ... results indicated that a simple paragraphbased algorithm that was intended as a baseline performed very poorly, and that word -based and some coherence -based algorithms showed the best performance ... previous sections are solely based on layout or on properties of word distributions in sentences, texts, and document collections Other approaches to sentence ranking are based on the informational...

Ngày tải lên: 17/03/2014, 06:20

... sensitivity, high specificity and the short incubation time needed to detect phosphorylation, this antibody -based approach presents obvious advantages over other existing methods, and may find wider uses ... In addition, a novel fluorescence -based approach for quantitative detection of peptide phosphorylation on chip using fluorescently-labeled anti-phosphoserine and antiphosphotyrosine antibodies ... high-throughput screenings of kinase activities, and potentially other enzymatic activities The intercalation of various PEGs between the slide surface and the immobilized peptides minimized non-specific...

Ngày tải lên: 16/09/2015, 17:14

... MBP and GST, and probed with Cy3-anti-EGFP, Cy5-anti-MBP and FITC-anti-GST, respectively Three corresponding non-biotinylated proteins were also spotted onto the same slide, as controls, and ... N-terminal cysteine and the thioester function of any other compound EPL represents a novel semisynthetic approach that has greatly expanded the utility of native chemical ligation chemistry and allows ... purify and label at its C-terminal by flushing the column with the cysteine containing labeling agent The expression and biotinylation of proteins was performed by Lue Yee Peng Rina, and cysteine...

Ngày tải lên: 16/09/2015, 17:14

... substrates to the microwells and incubated them with the kinase and radioactively-labeled ATP After phosphorylation had taken place, the kinases and ATP were washed away and the microwells scanned ... acids and peptides Both phosphorylated and non-phosphorylated tyrosine amino acids were spotted on an amine functionalized glass slide, and detected using FITC-labeled anti- phosphotyrosine and ... functionalized with (a) NHS PEG and (b) Amine PEG, and probed with FITC-labeled anti-phosphotyrosine 3.2.3 Rapid Microarray -Based Kinase Assay Rapid fluorescent antibody -based detection was combined...

Ngày tải lên: 16/09/2015, 17:14

... valves and hydrophobic vents for sample introduction and immobilization during thermal cycling.17 They used DNA sample of very low concentration and observed the stochastic PCR amplification and ... 33 Chapter the same time and Nagai et al reported a high throughput PCR amplification in a silicon based array.21 2.2 Results and Discussion 2.2.1 Method Development Based on Conventional Capillary ... Hydroxyethylcellulose (HEC) and 0.25 µg/mL TO, the two Z and W female bird genes 34 Chapter could be baseline resolved at 12kV as shown in Figure 2.7 Similarly to the mammalian X and Y genes, the Z and W genes...

Ngày tải lên: 16/09/2015, 17:14

... efficient size based separation of nucleic acids Matrix-free DNA analysis has also been reported and a nanofluidic channel was designed and fabricated to separate long DNA molecules based on the ... microchip based electrophoretic device The advantages conferred by such microfluidic -based systems are numerous and wide ranging The miniaturization leads to less reagent consumption, and ultimately ... microstructure Micro scale -based separations thus offer improved speed and efficiency compared to conventional electrophoretic -based separations The channel dimensions and flow rates typically...

Ngày tải lên: 16/09/2015, 17:14

... minireviews by K Tomizaki et al [33] and M Umetsu et al [34] deal with array based- technologies for detecting protein interactions in vitro, and constructive approaches to the generation of novel ... signaling -based systems, which require the incubation of yeast cells at suboptimal temperatures (25 and 36 °C), and the monitoring or discrimination of the signaling changes through quantitative and ... example, interactions of attractive drug target candidates, syntaxin 1a and nSec1 or fibroblast-derived growth factor receptor and SNT-1, were monitored, and nSec1 mutants that lost the ability to bind...

Ngày tải lên: 06/03/2014, 11:20

... T K Chaudhuri and S Paul and non-native states of targeted proteins, but how they discriminate between correctly and incorrectly folded proteins and how they selectively retain and target the ... causes aggregation and cell death in vitro and in vivo In vitro cellular models and transgenic animals have been created and 1336 analyzed with the truncated ataxin-3 with an expanded polyglutamine ... Peptidomimetics, based on the peptide LVFFA from Ab, modified at the N- or C-terminus, and the all-d (right-handed) version and several retro1342 inverso peptidomimetics, block both Ab seeding and growth...

Ngày tải lên: 07/03/2014, 12:20

... Gorrell, and J Boye 200 lb Plug and play spoken language understanding In Proceedings of SIGDIAL 2001, Aalborg, Denmark A Stent, J Dowding, J Gawron, E Bratt, and R Moore 1999 The CommandTalk ... error rate was affected by the following factors: • Use of rule based features only, N-gram based features only, or both rule and N-gram based features Data Rules NGrams Both 10% 20% 30% 40% 50% 60% ... conclusions We have presented a simple speech understanding framework combining rule -based and datadriven methods, which has been implemented and Data Rules NGrams Both 10% 20% 30% 40% 50% 60%...

Ngày tải lên: 08/03/2014, 21:20

... disciplines such as epidemiology and biostatistics typically use problembased exams and case studies, while behavioural sciences often use essay -based and oral examinations and professional programmes ... planned changes and involved in the transition process Prioritize within constraints and imperatives With this information in hand and a positive assessment of support within the college and university ... curriculum (expanded beyond that typical of MPH programmes) followed by courses designed to facilitate the integration and application of the new knowledge and skills in both practice and research...

Ngày tải lên: 18/06/2014, 17:20

... Advances in Robust Control – Novel Approaches and Design Methods, Edited by Andreas Mueller p cm ISBN 978-953-307-339-2 free online editions of InTech Books and Journals can be found at www.intechopen.com ... equilibrium position and F = u is the force acting on the mass, with m the mass, c the damper constant and k the spring constant 10 Recent Advances in Robust Control – Novel Approaches and Design Methods ... Ciprian Lupu Chapter 16 Partially Decentralized Design Principle in Large-Scale System Control 361 Anna Filasová and Dušan Krokavec Chapter 17 A Model-Free Design of the Youla Parameter on the...

Ngày tải lên: 19/06/2014, 08:20

... – Novel Approaches and Design Methods * − t Where Dij = P2 Ai + Ait P2 + WiC j + C tj Wit + ε ij 1K tj Ebi Ebi K j Thus, conditions (12) and (13) yield for all i, j from (25) and (26) and by using ... case of delay being constant and unknown and no uncertainties (ΔAi = 0, ΔAτi = 0), the existing delay-dependent approaches are used to design the fuzzy controllers Based on theorem 3, for λ = ... topic, we refer the reader to (Tanaka & al 1998 and Wand & al, 1995) and the references therein The stability analysis and the synthesis of controllers and observers for nonlinear systems described...

Ngày tải lên: 19/06/2014, 08:20

... Advances in Robust Control – Novel Approaches and Design Methods Figures and respectively show the behaviour of error e1 (t ) and e2 (t ) with and without the H ∞ approach and also the behaviour obtained ... Control – Novel Approaches and Design Methods Lauber J (2003) Moteur allumage commandé avec EGR: modélisation et commande non linéaires, Ph D Thesis of the University of Valenciennes and Hainault-Cambresis, ... CPU 2.40 GHz, and 504 MB of RAM, and software specifications of MS Windows XP 2002 OS and Matlab 6.5 simulator 4.1 Model reduction using neural -based state transformation and lmi -based complete...

Ngày tải lên: 19/06/2014, 08:20

... Control – Novel Approaches and Design Methods [12] K Gallivan, A Vandendorpe, and P Van Dooren, “Sylvester Equation and ProjectionBased Model Reduction,” Journal of Computational and Applied Mathematics, ... )⎤ds where t0 and t f are ⎦ 2t ⎣ 96 Recent Advances in Robust Control – Novel Approaches and Design Methods the initial time and the final time, respectively; and S(t f ) ≥ , Q ≥ , and R ≥ with ... simple pole placementbased state feedback controller can be designed For example, assuming that a controller is 84 Recent Advances in Robust Control – Novel Approaches and Design Methods needed...

Ngày tải lên: 19/06/2014, 08:20

... and load resistance variations 118 Recent Advances in Robust Control – Novel Approaches and Design Methods Among the various switching control methods, PWM which is based on fast switching and ... Advances in Robust Control – Novel Approaches and Design Methods come together in an organized and cooperative manner under the unified intelligent control design framework to meet the Chapter’s ... system design because the real engineering systems are vulnerable to external disturbance and measurement noise and there are always differences between mathematical models used for design and the...

Ngày tải lên: 19/06/2014, 08:20

... Approaches and Design Methods controller that makes impossible in selecting hardware and leads to premature failure of hardware The values for λ and in Eq (17) and Eq (20) are chosen as 20 and ... robust to parameter 152 Recent Advances in Robust Control – Novel Approaches and Design Methods uncertainties and disturbances Design of such systems includes two steps: (i) choosing a set of ... are many parameters and nonlinearities Nonlinear effects can better be understood by varying the parameters and 156 Recent Advances in Robust Control – Novel Approaches and Design Methods examining...

Ngày tải lên: 19/06/2014, 08:20

... Novel Approaches and Design Methods Theorem Consider the process (3) with limited uncertainties and assigned design values ˆ ˆ of K v and δˆr −d If there exist a set of reference poles P and a ... Novel Approaches and Design Methods Fig Four case two output responses of y1 and y2 (i)ideal sliding output, (ii) with no uncertainty and no disturbance, (iii)with matched uncertainty and matched ... (iii)matched uncertainty/disturbance, and (iv)unmatched uncertainty and matched disturbance 234 Recent Advances in Robust Control – Novel Approaches and Design Methods Fig Four phase trajectories...

Ngày tải lên: 19/06/2014, 08:20

... one constituted of the LR and the goodput (cf Section 4.1) and the second constituted of the LR and a TCP-compatible bandwidth share measure (cf Section 4.2) Both LR and bandwidth characteristics ... receivers and the receiver clustering mechanism governing the feedback aggregation are described, respectively, in Sections and Section describes the multilayer source and channel rate control and ... lead to periodic 160 congestion and periodic losses [15] PLM (Packet-pair layered multicast) [16] is a more recent layered multicast congestion control protocol, based on the generation of packet...

Ngày tải lên: 23/06/2014, 01:20

... Seismic Design Philosophies and Performance -Based Design Criteria 37.1 37.2 Introduction Design Philosophies No-Collapse -Based Design • Performance -Based Design 37.3 No-Collapse -Based Design Approaches ... Approach) Design philosophy Design load Design approach Analysis Design Force Ductile component Nonductile component Foundation Design displacement Concrete and steel design Foundation design For ... Bridge Design Specifications [26] have not been formally revised since 1989, project-specific criteria and design memoranda have been developed and implemented for the design of new bridges and the...

Ngày tải lên: 08/07/2014, 12:20