cells cancer stem cells and hepatocellular carcinoma

... anti-tumor and protective effects Our study aimed to estimate different responses of hepatic cancer cells and hepatocytes to the combination of nsPEFs and baicalin Cell viability, apoptosis and necrosis, ... [11, 13, 14] and diverse signal kinase pathways activation in cancer cells [15-17], and the ablation effect of nsPEFs has been validated on various malignancies including hepatocellular carcinoma ... Research Paper Dual-function of Baicalin in nsPEFs-treated Hepatocytes and Hepatocellular Carcinoma cells for Different Death Pathway and Mitochondrial Response Yubo Wang1*, Shengyong Yin1*, Yuan Zhou1*,

Ngày tải lên: 16/01/2020, 02:22

... EpCAM-positive HCC cells also express markers associated with cancer stem cells and the epithelial to mesenchymal transition, supporting a hypothesis that EpCAM enrichment identifies stem-like cells with ... concept, design, and protocol, consented and enrolled patients, managed and analyzed data, and wrote manuscript MJ-MM performed CTC and WGA assays and contributed to data analysis and writing TMB ... low-frequency variants and lower coverage depth than FFPE or PBMC Keywords: Hepatocellular carcinoma (HCC), Circulating tumor cells (CTC), EpCAM, Sequencing Background Hepatocellular carcinoma (HCC)

Ngày tải lên: 30/09/2020, 11:17

... antigens, and IFN-g-producing CD4þTh1-cells and CD8þT-cells are the principle immune cells responsible for inhibiting tumor growth and development Tregs are mainly induced from CD4þCD25T-cells in ... infection and HCC, as well as the host's response to antiviral and antitumor therapies.21Considering the substantial role of Tregs in immune responses against HBV and cancer cells, understanding ... dysregulation and tumori-genesis in HCC IFN-g-producing CD4þT helper 1 (Th1) cells and CD8þT-cells are believed to be the primary immune cells responsible for limiting tumor growth and development

Ngày tải lên: 04/12/2022, 16:03

... XTH and LMZ collected 56 patients’ samples and extracted tissue RNAs, GYW, FLD, ZYX and DFC carried out gene expression, GYW, JPZ, FLD, SS and QHD analyzed the data, QHD and JPZ conceived and ... homeostasis and metabolic functions MiR-122 targets CUTL1 and CCNG1, and loss of miR-122 results in blocked differentiation, genomic instability, and inflammation associated with liver disease and HCC ... and 11 other genes that are implicated in the occurrence of HCC and HBV infection Methods Patients and samples Tumor and paired adjacent non-tumor tissue samples were obtained from 56 liver cancer

Ngày tải lên: 06/08/2020, 03:28

... tumor-initiating cells are the founding cells of an arising tumor These undifferentiated self-renewing cells are the propagating pool responsible for tumor growth [30] Cancer stem cells (CSC) seem ... propagating neural stem cells Culture in serum-free medium containing bFGF and EGF promotes the growth of undifferentiated stem cells that form neurospheres and show self-renewal and exponential long-term ... culture and storage of the tumor cells and establishment of a stable tumor cell line It also includes genetic characterization and genetic alteration (such as lentiviral transduction) and use

Ngày tải lên: 21/09/2020, 11:46

... basal-like breast cancer cell progression5 Expression of FBP1 is downregulated in gastric and colon cancer cells6,7, and its loss associates with poor prognosis of clear cell renal cell carcinoma8 This ... expression in HCC cells HepG2 and SK-Hep1 cells were treated with different dosages of HDAC inhibitors including NaBu (A), SAHA (B) and LBH589 (C) for 24 hours Cells were harvested and FBP1 mRNA ... expression in HCC cells, we focused our attention on HDAC1 and HDAC2 among the 18 different members of the family because they are often deregulated in human cancers HepG2 and SK-Hep1 cells were transfected

Ngày tải lên: 04/12/2022, 14:57

... AccessIdentification of a unique hepatocellular carcinoma line, Li-7, with CD13(+) cancer stem cells hierarchy and population change upon its differentiation during culture and effects of sorafenib Takeshi ... biosciences) and FACSDiva software version 6.1 (BD biosciences) Doublet cells were eliminated using FSC-H and FSC-W, SSC-H and SSC-W Dead cells were eliminated as 7-AAD-positive cells For the positive and ... metastasis and recurrence of cancers [2,3] This new CSC model has deepened our understanding of the complexity of tumor tissues [4] Hepatocellular carcinoma (HCC) is one of the major causes of cancer-related

Ngày tải lên: 29/09/2020, 16:01

... Iwama A Cancer stem cells in hepatocellular carcinoma: recent progress and perspective Cancer Lett. 2009;286:145 –53. 6 Lobo NA, Shimono Y, Qian D, Clarke MF The biology of cancer stem cells. ... tumorigenic liver cancer stem/ progenitor cells Gastroenterology 2007;132:2542 –56. 9 Yoon SK The biology of cancer stem cells and its clinical implication in hepatocellular carcinoma Gut Liver ... vivo Keywords: Hepatocellular carcinoma, Wnt/β-catenin signaling, Cancer stem cells, Epithelial cell adhesion molecule (EpCAM), Tumor initiating cells Background Hepatocellular carcinoma (HCC)

Ngày tải lên: 03/07/2020, 01:24

... bladder urothelial carcinoma: similarities and differences between biopsy samples and cancer stem-like cells BMC Cancer 2014 14:646. Submit your next manuscript to BioMed Central and take full advantage ... complexity of the bladder cancer genome, and for the first time to our knowledge, on the possibility to compare genomic alterations of in vitro selected cancer stem-like cells (CSCs), and their original ... number alterations, cancer stem like-cells Background Bladder cancer is the fourth most common cancer in men and the eighth in women in both incidences and mortality and over 90% of bladder tumors

Ngày tải lên: 14/10/2020, 15:19

... [17,19-22] On the other hand, recent breast cancer studies have revealed the plasticity of cancer cells in which differentiated cancer cells can transform to attain cancer stem-like properties via ... cycle, drug efflux, and ROS Keywords: Cancer stem cell, HCC, Sphere, Chemoresistance Background Hepatocellular carcinoma (HCC) is the sixth most common and the third most deadly cancer worldwide ... this study, we induced cancer stem-like cells from hepatocellular carcinoma (HCC) cell lines using a unique medium, and examined their potential for resistance to anti-cancer drugs Methods: The

Ngày tải lên: 14/10/2020, 16:14

... growth, and invasion of cancer are driven by a small population of cancer stem cells (CSC) Previous studies have identified CD44+ cells as cancer stem cells in head and neck squamous cell carcinoma ... Dirks P, Eaves CJ: Cancer stem cells: an evolving concept Nat Rev Cancer 2012, 12:133 –143. 5 Reya T, Morrison SJ, Clarke MF, Weissman IL: Stem cells, cancer, and cancer stem cells Nature 2011, ... identified CD24 as a marker in cancer stem cells in several cancers, including pancreatic cancer [11], colorectal cancer-derived cell lines [8], and ovarian cancer [9] Cancer stem cell immunophenotype

Ngày tải lên: 05/11/2020, 01:35

... growth, and invasion of cancer are driven by a small population of cancer stem cells (CSC) Previous studies have identified CD44+ cells as cancer stem cells in head and neck squamous cell carcinoma ... Dirks P, Eaves CJ: Cancer stem cells: an evolving concept Nat Rev Cancer 2012, 12:133 –143. 5 Reya T, Morrison SJ, Clarke MF, Weissman IL: Stem cells, cancer, and cancer stem cells Nature 2011, ... identified CD24 as a marker in cancer stem cells in several cancers, including pancreatic cancer [11], colorectal cancer-derived cell lines [8], and ovarian cancer [9] Cancer stem cell immunophenotype

Ngày tải lên: 02/11/2022, 11:41

... Targeting cancer stem cells is considered an important approach in cancer treatment However, the similarity between stem cells and cancer stem cells can cause mistargeting between stem cells and cancer ... selective inhibition of breast cancer stem cell-dendritic cells and mesenchymal stem cell-dendritic cells on breast cancer stem cells The results show that a mixture of DCs and CTLs effected BCSC proliferation ... drug-resistant cancer cells as well as cancer stem cells. Keywords: dendritic cells, 4T1 cell line, breast tumor, breast cancer stem cells, verapamil, drug resistance Introduction Breast cancer is

Ngày tải lên: 16/12/2017, 00:33

... HCC cells- reactive T cells are induced by fusion cells, T cells from healthy donors were stimulated by fusions of DCs from the same healthy donors (HLA-A2+) and the HCC cells (HLA-A2+, WT1+, and ... nonadherent cells were removed and the adherent cells were cultured overnight To generate immature DCs (DCs), the nonadherent and loosely adherent cells were collected on the next day and placed ... penicillin, and 0.1 mg/ml streptomycin DCs alone, the HCC cells alone, an unfused mixture of both DCs and the HCC cells were used as controls T cells were purified with nylon wool and cultured...

Ngày tải lên: 18/06/2014, 15:20

... characterized miRNAs and is implicated in the tumorgenesis of several types of human cancers including B-cell lymphoma, breast cancer, colon cancer, lung cancer and pancreatic cancer Our data from ... HBsAg expression in (A) PLC/PRF/5 cells and (B) 2.2.15 cells …………………………………… 89 Figure 4.4 Effect of siRNA on HBsAg RNA in (A) PLC/PRF/5 cells and (B) 2.2.15 cells ……………………………………………………………… 91 ... might aid in cancer diagnosis and understanding of miRNA function in cancers may provide therapeutic targets Keywords: Short interfering RNA, microRNA, hepatocellular carcinoma, hepatitis B, expression...

Ngày tải lên: 14/09/2015, 12:11

... c-glutamylcysteine synthetase [14] and glutathione transferase [15] in cancer cells Such alterations of the glutathione system can enhance survival of cancer cells In general, hypoxia and reoxygenation may ... antioxidant system, in particular glutathione metabolism, and on studying DNA damage The metabolism of glutathione and DNA breaks were investigated in hepatocellular carcinoma HepG2 cells cultivated ... HepG2 cells during hypoxia and after reoxygenation HepG2 cells were incubated for h under normoxic and hypoxic conditions in the absence and presence of pyruvate (0.8 mm) Thereafter, hypoxic cells...

Ngày tải lên: 18/02/2014, 16:20

... lower levels of uPA and c-met proteins and mRNAs were detectable in AB2 normal cells and in the differentiated HCC cells, HepG2 and HuH6 cells (Fig 2A,C) These data Fig The uPA and c-met 3¢-UTRs, ... uPA and c-met protein expression in AB2 cells Western blot analysis (A) of uPA in the conditioned media of control cells (lanes 1, 2, 4, and 5) and anti-miR-23b-transfected cells (lanes and 6) ... total RNA isolations from control cells (lanes 1, 2, 4, and 5) and transfected cells (lanes and 6) were examined by RT-PCR to detect uPA mRNA (A) and c-met mRNA (B), and by real-time PCR to detect...

Ngày tải lên: 29/03/2014, 23:20

... CD44, CD24, ESA and ALDH1 in different cancers [1] Few of these markers were used for the confirmation of an MUC4 enriched cancer stem cell population Stem cells and cancer stem cells are known ... overexpressed ovarian cancer cells induce the aggressiveness of the cancer cells and enrich the cancer stem cell population It also showed that MUC4 is expressed in the ovarian cancer stem cell population ... Batra SK: Ovarian cancer: emerging concept on cancer stem cells J Ovarian Res 2008, 1:4 Dean M, Fojo T, Bates S: Tumour stem cells and drug resistance Nat Rev Cancer 2005, 5:275-284 Andrianifahanana...

Ngày tải lên: 20/06/2014, 07:20

... Telmer and Eva Turley Breast Cancer Stem Cells 235 Chapter 11 The Microenvironment of Breast Cancer Stem Cells Deepak Kanojia and Hexin Chen Chapter 12 Involvement of Mesenchymal Stem Cells in ... of breast cancer cells, molecular tumor classification methods, in vitro cancer models, breast cancer and microenvironment, breast cancer stem cells, gene regulation in breast cancer, and mechanism ... Muehlberg, F., Beckmann, C and Song, Y H (2009) Tissue resident stem cells produce CCL5 under the influence of cancer cells and thereby promote breast cancer cell invasion Cancer Lett, Vol 284, No.1:...

Ngày tải lên: 27/06/2014, 17:20

... expression profiling of cancer stem cell in human lung adenocarcinoma A549 cells Mol Cancer 2007, 6:75 19 Pfeiffer MJ, Smit FP, Sedelaar JP, Schalken JA: Steroidogenic enzymes and stem cell markers ... AK1C3 [19] and CD44 [20] have been proposed to be the markers of cancer stem cells Their expression in tumor cells could correlate with their aggressive biological behavior, drug resistance and poor ... variety of malignant tumor cells In particular, AK1C1, and AK1C3 are reported in human non-small cell lung carcinoma (A549) cells [21], and a high expression of AL1A1 in lung cancer cell lines, especially...

Ngày tải lên: 10/08/2014, 09:22