cell surface engineering of mesenchymal stem cells

... interventions28 Mesenchymal Stem Cells In The Treatment Of Acute Myocardial Infarction With the emergence of stem cells as potential regenerative medicine, attempts to use stem cells to reduce ... 1CHARACTERIZATION OF THE SECRETION OF MESENCHYMAL STEM CELLS AND ITS RELEVANCE TO CARDIOPROTECTION LAI RUENN CHAI NATIONAL UNIVERSITY OF SINGAPORE 2011 Trang 2   CHARACTERIZATION OF THE SECRETION OF MESENCHYMAL ... 2004 Mesenchymal stem cells and their potential as cardiac therapeutics Circ Res 95, 9–20 Quesenberry, P.J., Aliotta, J.M., 2008 The paradoxical dynamism of marrow stem cells: considerations of stem

Ngày tải lên: 10/09/2015, 08:42

... minor axes of the cells were computed from the moments up to the second order of the thresholded binary image of the cell using NIH ImageJ; the aspect ratio of the cell is the ratio of major to ... producing a high level of desired lineages from the stem cells Thus, intense efforts have been dedicated to the identification of physical contributors in the regulation of stem cell behaviors [5-7] ... analysis of cell adhesion, 1.0 x 104 cells/cm2 were seeded each well in a 24-well plate and allowed to attach for 24 hours Then, the cells were washed 3 times with PBS to remove non- adherent cells,

Ngày tải lên: 15/01/2020, 00:46

... Disease Model Systems Stem Cells 2011; 29: 920-7 5 Dasari VR, Veeravalli KK, Dinh DH Mesenchymal stem cells in the treatment of spinal cord injuries: a review World J Stem Cells 2014; 6: 120-33 ... Mesenchymal stem cells inhibit the differentiation of dendritic cells through an interleukin‐6‐dependent mechanism Stem Cells 2007; 25: 2025-32 40 Ehninger A, Trumpp A The bone marrow stem cell ... I-K, Kim B-S Mesenchymal stem cells for treatment of myocardial infarction International journal of stem cells 2008; 1: 49 10 Satessa G, Lenjisa J, Gebremariam E, Woldu M Stem cell therapy

Ngày tải lên: 15/01/2020, 09:12

... Luo X, et al A comprehensive analysis of the dual roles of BMPs in regulating adipogenic and osteogenic differentiation of mesenchymal progenitor cells Stem Cells Dev 2009; 18: 545-59 49 Luo J, ... mesenchymal stem cells Science 1999; 284: 143-7 12 Lamplot JD, Qin J, Nan G, Wang J, Liu X, Yin L, et al BMP9 signaling in stem cell differentiation and osteogenesis Am J Stem Cells 2013; ... Potential of Human Osteosarcoma Cells Cell Physiol Biochem 2013; 32: 1083-96 54 Zhao C, Wu N, Deng F, Zhang H, Wang N, Zhang W, et al Adenovirus-mediated gene transfer in mesenchymal stem cells

Ngày tải lên: 15/01/2020, 20:53

... in the field of tissue engineering, as can be seen in the Table 1.1 Table 1.1 Polymers used for nanofibers to support stem cells 1.1.4 Mesenchymal stem cells Mesenchymal stem cells, first ... marrow cells have the ability to differentiate into cartilage or muscle cells Due to the ability to differentiate into different cell lines of these cells, the name "Mesenchymal stem cells" ... section 18 2.2.1 Fabrication of gelatin nanofibers 18 2.2.2 Human Mesenchymal Stem Cell (hMSC) studies 23 2.2.3 The combination ability of bionanomembrane and cells 28 CHAPTER 3: RESULTS

Ngày tải lên: 22/12/2020, 22:04

... in the field of tissue engineering, as can be seen in the Table 1.1 Table 1.1 Polymers used for nanofibers to support stem cells 1.1.4 Mesenchymal stem cells Mesenchymal stem cells, first ... marrow cells have the ability to differentiate into cartilage or muscle cells Due to the ability to differentiate into different cell lines of these cells, the name "Mesenchymal stem cells" ... section 18 2.2.1 Fabrication of gelatin nanofibers 18 2.2.2 Human Mesenchymal Stem Cell (hMSC) studies 23 2.2.3 The combination ability of bionanomembrane and cells 28 CHAPTER 3: RESULTS

Ngày tải lên: 06/02/2021, 19:38

... Preconstruction of a vascular network through coculture of osteogenic cells (osteoblasts or mesenchymal stem cells (MSCs)) and vasculogenic cells (endothelial progenitor cells or endothelial cells (ECs)) ... expressed by the two types of cells According to the flow cytometry results of CD31, the negative cells contained only 0.03% CD31+ cells, and HUVECs reached 89.24% of positive cells after MACS sorting ... endothelial cells synergistically promote osteogenesis of mesenchymal stem cells via integrin β1-FAK-ERK1/2 pathway State Key Laboratory of Bioreactor Engineering, East China University of Science

Ngày tải lên: 12/01/2022, 22:32

... types of somatic cells along with follicular fluid [1] The main types of aspirated follicular cells (AFCs) are represented by granulosa cells (GCs) and theca cells (TCs) The main role of GCs ... commercially available cell line of bone marrow-derived mesenchymal stem cells (BM-MSCs) was used (Chemicon, Millipore, cat number SCC034) These cells were cultured in a mesenchymal stem cell expansion ... potential relation of AFCs to mesenchymal stem cells (MSCs), the most common cells tested in the regeneration of impaired ovarian function in the animal models [12,13] The aim of this study was

Ngày tải lên: 02/11/2022, 10:44

... loss1 Periodontal ligament stem cells (PDLSCs), a population of mesenchymal stem cells (MSCs), were 1 Department of stomatology, Daping hospital, Research institute of Field surgery, Third military ... technique All of cell lines are identified by flow cytometry to analyze the cell surface markers and by multi- differentiation experiment All of cell lines expressed mesenchymal stem-cell markers ... coherent feed-forward loop in mesenchymal stem cells isolated from periodontal ligaments of patients with periodontitis Stem cells 29, 1804–1816, doi: 10.1002/stem.728 (2011). 16 Ding, G et

Ngày tải lên: 24/11/2022, 17:43

... SEM, the damage to the connections of endothelial cells of venules was increased over time after induction of ANP The gaps between connections of endothelial cells progressed at each time point, ... connections of venule endothelial cells increased over time after induction of ANP (b, ANP 6 h; c, ANP 12 h; d, ANP 24 h) The gaps between connections of endothelial cells at 24 h was the most wide of ... distribution on culture plates (Fig 1a) The cells were identified by their surface markers High expression of CD 29/90 and low expression of CD 34/45 confirmed that these cells were MSCs (Fig 1c–f) After

Ngày tải lên: 24/11/2022, 17:56

... University of Milan, Milan, Italy Full list of author information is available at the end of the article Abstract Background: Mesenchymal stem cells (MSCs) might act as fine-tuners of inflammation ... by PTX3-deficient MSCs (Table 1) Fig 2 Early effects of mesenchymal stem cells (MSCs) on oxygenation, lung edema, and alveolar inflammatory cells in acid aspiration acute lung injury Wild-type ... Additional file 1 of this article Results Mesenchymal stem cells enhance short- and long-term recovery from experimental acid aspiration acute lung injury In 24 h, i.p administration of WT-MSCs 1

Ngày tải lên: 04/12/2022, 15:02

... marrow-derived mesenchymal stem cells (BMMSCs) have a great potential of proliferation and differenti-ation, and they have been considered as a suitable source for cell therapy [5, 6] Mesenchymal stem cells ... differentiation of MSCs Differentiation of MSCs to cardiogenic cells was accom-plished afterwards MSCs of the three groups were cells per well To induce cell differentiation, the cells were incubated ... non-adherent cells were re-moved The adherent cells were washed two times gently with phosphate-buffered saline (PBS) to reduce the degree of hematopoietic lineage cell contamination The cells were

Ngày tải lên: 04/12/2022, 15:12

... human placenta Stem Cells 22, 1338–1345 Bieback K, Kern S, Kluter H & Eichler H (2004) Critical parameters for the isolation of mesenchymal stem cells from umbilical cord blood Stem Cells 22, 625–634 ... specific features of the plasticity of adult stem cells are largely unknown Recently, we demonstrated the hepatic differentiation of human adipose tissue-derived mesenchymal stem cells (AT-MSCs) ... scalp tissue Stem Cells 23, 1012–1020 In’t Anker PS, Scherjon SA, Kleijburg-van der Keur C, de Groot-Swings GM, Claas FH, Fibbe WE & Kanhai HH (2004) Isolation of mesenchymal stem cells of fetal

Ngày tải lên: 30/03/2014, 04:20

... delivery of CX3CL1 to multiple lung tumors by mesenchymal stem cells Stem Cells 2007, 25(7):1618-26 12 Komarova S, Kawakami Y, Stoff-Khalili MA, Curiel DT, Pereboeva L: Mesenchymal progenitor cells ... the level of cells that home to tumors or other sites The tumor homing behavior of MSCs was demonstrated by the mere presence of these cells in the sites of interest and/or lack of such cells in ... phenomenon of MSCs was confirmed in different experimental systems [2,12] Other cell types, such as umbilical cord matrix stem cells (UCMS) [14], neural stem cells [15,16] and endothelial progenitor cells...

Ngày tải lên: 20/06/2014, 07:20

... accelerated degeneration of the treated IVD [18-24] An alternative cell source are adult stem cells, in particular, bone-marrow-derived mesenchymal stem cells (MSCs) The use of these cells would allow ... Intervertebral disc cell- mediated mesenchymal stem cell differentiation Stem Cells 2006, 24:707-716 27 Richardson SM, Hughes N, Hunt JA, Freemont AJ, Hoyland JA: Human mesenchymal stem cell differentiation ... E, Bertram H, Abel R, Chen B, Winter A, Richter W: Induction of intervertebral disc-like cells from adult mesenchymal stem cells Stem Cells 2005, 23:403-411 44 Risbud MV, Albert TJ, Guttapalli...

Ngày tải lên: 09/08/2014, 01:22

... L-selectin)/lymphohematopoietic stem and progenitor cells, small-vessel endothelial cells, embryonic fibroblasts Augments B responses/ plasma cells Growth, differentiation, and isotype-switching of B cells/ B cells Ligand ... marrow-derived mesenchymal stem cells and neural progenitor cells Haematologica 2003, 88:126-133 Reyes M, Verfaillie CM: Characterization of multipotent adult progenitor cells, a subpopulation of mesenchymal ... Ligand for CD28 and CD152 (CTLA-4)/B cells Growth factor receptor/haemopoietic stem and progenitor cells, mast cells Function unknown/haematopoietic stem cells and precursors [67] [67,68] [67]...

Ngày tải lên: 09/08/2014, 08:23

... Expression levels of ADAM and adhesion molecules during chondrogenic differentiation of mesenchymal stem cells (MSCs) The gene-expression (MSCs) profile of mesenchymal stem cells (MSCs; n = 2) ... Page of 12 (page number not for citation purposes) Arthritis Research & Therapy Vol No Djouad et al Figure stem cells of the expression of cell- surface proteins on mesenchymal Modulation(MSCs) stem ... 39 40 41 42 43 44 45 46 47 48 49 characterization of mesenchymal stem cells Stem Cells 2006, 24:475-481 Charo IF, Ransohoff RM: The many roles of chemokines and chemokine receptors in inflammation...

Ngày tải lên: 09/08/2014, 10:20

... migration of mesenchymal stem cells to conditioned medium from tumor cells or bone marrow cells Stem Cells 2007, 25:520-528 Reya T, Morrison SJ, Clarke MF, et al: Stem cells, cancer, and cancer stem cells ... ratio of MSCs to cancer cells, nature of tumour cells and cancer stem cells, integrity of immune system, number of stem cell passages and site of injection; all can affect the outcome of MSCs ... relationship between stem cells and tumor cells Qiao and coworkers reported that human mesenchymal stem cells (hMSCs) can home to tumor sites and inhibit the growth of tumor cells[ 8] Furthermore,...

Ngày tải lên: 10/08/2014, 10:21

... Characterisation of canine adipose tissue derived mesenchymal stem cells In order to demonstrate that the cells isolated from the canine adipose tissue (Figure 1A) are indeed mesenchymal stem cells (MSCs) ... morphological features of apoptotic cell death was determined by scoring 100 cells from 30 different microscopic fields per culture and the number of apoptotic cells expressed as an indicator of MSC culture ... the biofunctions and biotransformations of curcumin Biofactors 2000, 13:153-158 19 Conget PA, Minguell JJ: Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells...

Ngày tải lên: 12/08/2014, 14:22

... Figure Mesenchymal stem cells used during this study Mesenchymal stem cells used during this study Typical morphological aspects of mesenchymal stem cells observed through culture flask (A) Mesenchymal ... distribution of mesenchymal stem cells in vivo Figure Early dynamic distribution of mesenchymal stem cells in vivo Sequential whole body scintigraphies after infusion of indium-111 labeled mesenchymal stem ... perspective of cellular therapy by intravenous stem cells infusion The participation of stem cells in several vascular diseases pathogenesis was first proved with haematopoietic stem cells (HSCs)...

Ngày tải lên: 12/08/2014, 18:20