cell homeostasis and stress response

... sub-network) 1 Table 10 Proteins and network crosstalk values 4 Discussion 4.1 Wnt signaling in maintaining homeostasis and managing cellular stress Homeostasis, balance of cellular processes, is an ... cancer, mesothelioma, bladder cancer Tan and Kelsey, 2009; Paul and Dey, 2008; Gehrke, Gandhirajan and Kreuzer, 2009 GSK-3β colorectal cancer Ge and Wang, 2010 Table 3 The common proteins ... important phenomenon since cells are the factories that maintain the intracellular environment and keep the conditions stable Therefore, it is essential for cells to maintain homeostasis for the organism

Ngày tải lên: 22/06/2014, 03:20

... authors read and approved the final manuscript. Acknowledgements We thank Drs M Inouye and S Phadtare for the BX04 mutant cells and the pINIII vector and Dr R Landick for the E coli RL211 cells This ... RNA chaperone activity and is involved in rRNA processing, which is important for normal growth, devel-opment, and the stress response in plants Results Structural features and characterization ... during plant growth and development, the T-DNA insertion mutant lines in CFM4 (SALK_076439 and SALK_126978) were obtained, and their phenotypes were analyzed under normal and stress conditions

Ngày tải lên: 27/05/2020, 01:43

... cultures, andtraditional 2D culture controls, we evaluated PCa cell migration and proliferation in response to bone marrow stromal cell populations, as well as PCa cell response to Docetaxel and Abiraterone ... PCa cells, and then evaluation of PCa cell proliferation or drug response Using this novel 3D culture platform, we show that PCa cell response to drugs varies considerably in 2D and in 3D, and ... with PCa cells, and the mul-tiple medium exchanges required to study the interaction of cells and different drugs in these complex cultures Fig 5 C42B cell Docetaxel drug response in mono- and co-cultures

Ngày tải lên: 24/07/2020, 01:02

... significant in MCF-7 cells and MDA-MB-231 cells, suggesting that processes other than cell cycle arrest also contribute to reduced cell growth In MCF-7 and HCC1806 cells, the increase of cells in G0/G1was ... analyse cell cycle phase Each cell line showed distinct differences in baseline cell cycle profiles, with MCF-7 cells being mainly in G0/G1, HCC1806 cells in S phase, and MDA-MB-231 cells in ... and glutamine uptake in breast cancer cells Using three different breast cancer cell lines: estrogen-receptor (ER)-positive, Luminal A MCF-7 cells, triple-negative basal-like HCC1806 cells, and

Ngày tải lên: 24/07/2020, 01:45

... stress and xenobiotic metabolic responses Activation of NRF2 perturbs stress response and detoxification pathways in ESCC Enriched pathways involving stress response, xenobiotic metabolism, and ... pathways Pathway 20 (injury and abnormalities and cell cycle), pathway 21 (carbohydrate and lipid metabolism, and molecular transport), and pathway 22 (cell death and survival pathways) serve ... pairs of esophageal squamous cell tumors and matched control tissues Results: We found significant dysregulation of genes encoding drug-metabolizing enzymes and stress response components of the

Ngày tải lên: 06/08/2020, 07:36

... real-time cell analysis and cell migration assays were performed The effects of treatment with the calmodulin antagonists, trifluoperazine (TFP) and ophiobolin A, on cellular Ca2+homeostasis, cell ... SEC62 silencing and TFP treatment additively affect cell growth and migration in PC3 cells A, Cells were seeded in 6-cm dishes and transfected with SEC62 siRNA or control siRNA 24 h and 48 h after ... Again, HeLa cells were more sensitive to the treatments than PC3 cells To confirm the results, we tested different human lung (H1299, A549 and BC01) and thyroid cancer cell lines (BHT101 and ML1)

Ngày tải lên: 05/11/2020, 05:44

... JNK action in the cell stress response The JNK kinases are activated in response to several cellular stressors and in flammatory mediators, and JNK activation can either promote cell death or survival ... either cell survival or cell death in response to stress JNK action on cell survival in pathological conditions associated with obesity and type-2 diabetes such as NAFLD,b-cell death, and vascular ... driving cell death, was also observed in response to DNA damage [48e50,69] and, more recently, in response to endoplasmic reticulum stress[70,71] A general model for the role of JNK in stress response

Ngày tải lên: 04/12/2022, 14:55

... the experimental work and drafted the manuscript AB and IA helped in the RT-PCR and DD-PCR analysis SM and RWB together designed the experiments IA and SM had proof-read and finalized the manuscript ... amplified by different anchored and arbitrary primer pairs (Table 1; Figures 1A-F) The anchored and arbitrary are random decamer primers, and used as reverse and forward primer for cDNA synthesis ... DD12 (B1-B20) The bands eluted for analysis are indicated ( ®). Trang 4This suggests that the expression ofbC1 gene results in astress response and the plant responds to these stresses by increasing

Ngày tải lên: 11/08/2014, 21:21

... demand with an appropriate response This cognitive response came to be known as the "fight-or-flight" response [5] and involved the activation of necessary physiological and behavioral responses ... adreno-cortical response to anumber of somatosensory stimuli [42] Thus, the limbic system is actively involved in the body's stress response and this stress response is affected by memory and emotion ... noreadrenocorticotro-pinephrine and eadrenocorticotro-pinephrine [7] Once the stress response is activated, behavioral and physiological changes lead the way for the organism to adjust homeostasis within the body, and increase

Ngày tải lên: 13/08/2014, 14:20

... infection Since the effects of shear stress, tissue hypoxia, cell activation and cell injury are hardly distinguishable from Figure 5 Effects of shear stress and different conditions of activation ... numbers 315/99 and 69/2003-A) and informed consent, human umbilical venous endothelial cells (HUVEC) and polymorphonuclear neu-trophils (PMN) were derived from human umbilical veins and citrated ... cell damage and organ dysfunction, are there mechanisms, independent of the physiological immune response, that gain a leading role for the distribution of leukocyte accumulation? Materials and

Ngày tải lên: 13/08/2014, 21:21

... of the stress damage and cell survival, or alternatively apoptosis PERK promotes cell viability in response to ER stress, and loss of PERK induces cell death in pancreatic β-cells (9), indicating ... system 22 4C ATF5 and cell survival 22 4D ATF5 and its target genes 24 5 Role of eIF2 phosphorylation in disease .24 MATERIALS AND METHODS .28 Trang 8 2 Cell Culture and Stress Conditions ... between GCN2 and other stress response pathways The target of rapamycin (TOR) is a serine/threonine protein kinase and a sensor of cellular nutritional status in yeast and mammalian cells Rapamycin,

Ngày tải lên: 24/08/2014, 11:17

... 2001a). ABCA1 AND LIPID HOMEOSTASIS Normal cellular lipid homeostasis In normal metabolism, there is continuous traf-fic of cell phospholipid (PL) and free cholesterol (FC) to and from their ... similar impact on cell growth (Y Hamon unpublished) ABCA1 AND CELL TURNOVER: THE CLEARANCE OF CELLS DYING BY APOPTOSIS Apoptosis or programmed cell death is a genet-ically controlled and highly regulated ... lines and in primary cellular sys-tems in mouse, such as those of resident or elicited peritoneal and bone marrow derived macrophages and in humans in activated monocytes, macrophages and foam cells

Ngày tải lên: 29/12/2017, 16:40

... mitochondrial oxidative stress is governed by an ef-fective response, including antioxidant machinery and the repair of damaged cellular components [4] However, an oxidative stress response is energetically ... mitochondrial and stress response proteins in root tissue from untreated plants and plants treated with 50 μM selenite for 1 and 3 days 20 ug of denatured protein was loaded per lane and analyzed ... roots and likely impact respiration and primary metabolism Thus, the objective of this study was to better understand the metabolic adjustments that occur in roots in response to selenite stress

Ngày tải lên: 27/05/2020, 01:23

... biotic and abiotic stress response, but also regulate growth and development Grapevine (Vitis vinifera) production is largely limited by stressful climate conditions such as cold stress and the ... of stress, including pathogen exposure, salicylic acid, ethylene, cold and drought stress [32] VvWRKYs that were up-regulated in response to more than two types of stresses (e.g patho-gen and ... gene expression in plants during abiotic stresses such as cold [22,23], salt [24,25] and drought [26-28] Besides roles in response to biotic and abiotic stress, WRKY proteins were also implicated

Ngày tải lên: 27/05/2020, 01:45

... oxidative stress [11] In addition, the tobacco plants overexpressing AtUCP1 exhibited faster germination under control and stressful conditions, improved performance under drought and salt stresses, and ... investigated the mechanism of UCP1 action in the stress response We used molecular, cellular and genomic tools to investigate the molecular and cellular events resulting from the overexpression ... (GRPe chaperone) and energy metabolism (NADH-DeH, NADH-IS2, and NADH-IS7) in L1 leaves of WT and transgenic lines P49 and P07 (G) Immunoblot analysis of nuclear- (IDH, VDAC and AOX) and mitochondrial-encoded

Ngày tải lên: 27/05/2020, 02:01

... out by oligosaccharides, and particularly, by oligoglucans 2 Cell Metabolism – Cell Homeostasis and Stress Response Oxidative stress and reactive oxygen species Oxidative stress is defined as the ... understand common molecular responses to a given stressor The chapters in this book address stress response in yeast, plants and humans The topics discussed include acetic acid-induced stress response ... chloroplasmatic APX (in the stroma and thylakoid) (Ishikawa et al., 1996) and mitochondrial (De Leonardis et al., 2000) Each one, Cell Metabolism – Cell Homeostasis and Stress Response with a specific...

Ngày tải lên: 22/06/2014, 03:20

... peroxidases and catalases 16 Cell Metabolism – Cell Homeostasis and Stress Response The analysis of differential gene expression and analysis of global patterns of gene expression using macro and microarray ... pattern, whatever the nature of the stressor 14 Cell Metabolism – Cell Homeostasis and Stress Response Examples of non-specific indications of the state of stress are: increased respiration, ... Signal interactions between NO and reactive oxygen intermediates in the plant hypersensitive disease resistance 10 Cell Metabolism – Cell Homeostasis and Stress Response response Proceedings of the...

Ngày tải lên: 22/06/2014, 03:20

... temperatures Plant Cell Environmental, Vol.23, pp.893-902 38 Cell Metabolism – Cell Homeostasis and Stress Response Zhu, J-K (2001a) Cell signaling under salt, water and cold stresses Current ... superoxide anion radical (O2•−), hydrogen peroxide (H2O2), and hydroxyl radical (OH•) ROS also 40 Cell Metabolism – Cell Homeostasis and Stress Response include the singlet oxygen (1O2), which is not ... ОН• (2) 42 Cell Metabolism – Cell Homeostasis and Stress Response This reaction can also be catalyzed by univalent cuprous ion, which is oxidized to divalent ion Both the oxidized iron and cuprum...

Ngày tải lên: 22/06/2014, 03:20

... Currently, there is 78 Cell Metabolism – Cell Homeostasis and Stress Response increasing evidence that apoptotic-like cell death pathways exist in unicellular organisms such as yeast and that this ability ... elicited stress responses may be insufficient to guarantee cell survival and cell death may occur The simplicity of yeast and its amenability to manipulation and genetic tractability make this unicellular ... appears that in Z bailii both membrane transport and acetyl-CoA synthetase could assume particular 76 Cell Metabolism – Cell Homeostasis and Stress Response physiological relevance in regards to...

Ngày tải lên: 22/06/2014, 03:20

... yeast: the role of intracellular polyols J Gen Microbiol 1972; 72:589-591 110 Cell Metabolism Cell Homeostasis and Stress Response Campanella ME, Chu H & Low PS Assembly and regulation of a glycolytic ... Instituto Politộcnico Nacional Mexico ** Corresponding Author * 102 Cell Metabolism Cell Homeostasis and Stress Response and Fulton (Fulton, 1982) have suggested that enzymes are not dispersed ... ATP-G-actin is kept stationary (Fig 2) 104 Cell Metabolism Cell Homeostasis and Stress Response Upon incorporation to a filament, G-actin-bound ATP is hydrolyzed ADP and Pi remain non-covalently bound...

Ngày tải lên: 22/06/2014, 03:20