cell cycle transcription and translation

... level, play a central role in cell cycle regulation Cell differentiation converts dividing cells into non-dividing cells and also determines cell fate In many cases, cell differentiation occurs ... size and shape49 Plant morphogenesis relies on spatially and temporally coordinated cell proliferation and differentiation44 Cell proliferation is increase the number of cells through the cell ... through the cell growth and division producing daughter cells The core cell cycle regulatory mechanisms are conserved in eukaryotes The cell cycle consists of the replication phase and the mitotic phase,

Ngày tải lên: 19/11/2022, 11:49

... Materials and methods Human endothelial cells and proinflammatory cytokines Human aortic endothelial cells (HAEC) and human umbilical vein endothelial cells (HUVEC) (frozen vials of 106 cells) were ... of F11R mRNA in HAEC and HUVEC by real-time PCR HAEC and HUVEC endothelial cells were grown to con-fluence and treated with cytokines at various times and doses The treated cells were washed with ... (100 units/ ml) and/or IFNg (200 units/ml) for various periods of time Analysis of F11R in HAEC and HUVEC lysates and cell culture media Monolayers of arterial and venous endothelial cells (90 -95%

Ngày tải lên: 18/06/2014, 19:20

... cells more susceptible to radiation-induced DNA damage and, 3) downregulates total Chk1 and Cyclin B abrogating the radiation-induced G2/M checkpoint and allowing cells to re-enter the cell cycle ... Loratadine on In Vitro Cell Cycle Progression HT29 cells treated with loratadine (75 μM) for 24 hours. Loratadine was then washed off and cells were irradiated (8 Gy). Cell cycle progression was ... loratadine- treated cells clearly dominated the cell cycle delay and radiation with loratadine did not cause additional cell cycle delays. As shown in Figure 5B, the percent of cells in G2 did not

Ngày tải lên: 09/08/2014, 10:20

... six different FLS cell lines with eight per treat-ment group Cell cycle analysis and evaluation of cell death For cell cycle analysis FLS were harvested after 4, 8, 16, 24 and 48 hours after ... showed a strong accumulation of intracellular CML com-pared with cells receiving Co-BSA (Figure 1b) Cell proliferation, cell viability, cell cycle and evaluation of cell death To evaluate whether ... Percentage of cells in the subG1 and G1 phases of the cell cycle Incubation of fibroblast-like synovial cells (FLS) with AGE-BSA increased the percentage of cells in the subG1 and G1 phases

Ngày tải lên: 09/08/2014, 14:22

... trees resulting from the transcription and translation datasets (hereafter referred to as the 'transcription tree' and the 'translation tree') are shown in Figure 2a and 2b, respec-tively The ... transcription and translation datasets Figure 2 Unrooted maximum likelihood (ML) phylogenetic trees obtained from the transcription and translation datasets (a) Transcription; (b) translation The ... two parts: B' and B" The black and white boxes correspond to the B' and B" parts of the gene, respectively S and F represent the split and fusion event hypotheses of the B' and B" parts

Ngày tải lên: 09/08/2014, 20:21

... fibroblast cells, CEM-A human T cells, D17 canine osteosarcoma cells, and 293 human embryonic kidney cells. Forty-eight hours post-transduction, the transduced cells were harvested and Luc activity ... 1) improving translation of the internal transgene transcript; and 2) functionally synergizing with a transcriptional enhancer to achieve coordinate increases in RNA synthesis and translation. ... and cytomegalovirus immediate early enhancer (CMV IE). Results from transfected cells and four different transduced cell types indicate that: 1) PCE enhanced transgene protein synthesis; 2) transcription

Ngày tải lên: 13/08/2014, 09:21

... of cell cycle and cell differentiation In a developmental context, the cell cycle and cell differentiation are tightly coordinated both spatially and temporally Adequate crosstalk between cell ... coordinate cell cycle and cell differentiation, and several experiments have been conducted to test this hypothesis However the elaborate relationship between cell cycle and differentiation in multicellular ... muticellular organisms arise from a single cell During this process, the cell number increases by cell division and the cells become different from each other by cell differentiation How cell

Ngày tải lên: 12/09/2015, 09:28

... cell cycle progression 1.1.2.1 RB/E2F/DP-mediated transcriptional regulation of cell cycle progression The E2F family of transcription factors integrates cellular signals and coordinates cell ... T cells of TRIP-Br2 wildtype and mutant mice (lymph nodes) .241 Figure 46 B220+ B cells and CD11c+ dendritic cells of TRIP-Br2 wildtype and mutant mice (spleen) 242 Figure 47 B220+ B cells and ... of the cell cycle and cancer 25 1.1.1 The general schema of cell cycle progression: simplifying complexity 25 1.1.2 The control of cell cycle progression 27 1.1.2.1 RB/E2F/DP-mediated transcriptional

Ngày tải lên: 13/09/2015, 20:18

... the mechanism of cell growth inhibition Cell proliferation was analyzed by MTT assay, and the cell cycle and apoptosis were assessed by flow cytometry And we transfected the cells with the GFP-RFP-LC3 ... AccessBicyclol induces cell cycle arrest and autophagy in HepG2 human hepatocellular carcinoma cells through the PI3K/AKT and Ras/Raf/MEK/ERK pathways Yu Wang, Hao Nie, Xin Zhao, Yong Qin*and Xingguo Gong* ... inhibited cell cycle progression at G1 phase and induced autophagy in HepG2 cells, which implied that the significant decrease in cell proliferation was mainly induced by autophagy and inhibition of cell

Ngày tải lên: 20/09/2020, 18:01

... follicular cell lines, FTC133, FTC236, ML1 and TT2609, and the C643 and HTh7 ATC cell lines also had midrange IC50 values (2.9-3.2 μM and 3.1-3.2 μM, respectively) HTh83 ATC cells and FTC238 FTC cells ... increased the proportion of cells in subG1 peak and induced cell cycle arrest in thyroid carcinoma cells To investigate the effects of sorafenib on cell cycle distribution and on cell death-associated ... effects on the cell cycle Sorafenib treatment increased the proportion of cells in G1 and decreased the proportion of cells in S phase in all papillary cell lines (BHT101, B-CPAP and TPC1) and in the

Ngày tải lên: 30/09/2020, 11:14

... MM cells and inhibit STAT3 phosphorylation in U266 and MM1.S cells Next, we used western blotting to explore intracellular signaling proteins associated with cell survival in MM cells The cells ... cell proliferation in vitro and in vivo, and to elucidate the mechanisms of action of CaM antagonists, we have revealed two cellular and molecular mechanisms: induction of cell cycle arrest and ... cell cycle progression RPMI 8226, U266, and MM1.S cells were treated with ana-lyzed by flow cytometry Treatment with W-7 and W-13 increased the percentage of cells in the G0/G1 phase of the cell

Ngày tải lên: 14/10/2020, 13:10

... unrestricted use, distribution, and Trang 2DNA damage activates cell cycle checkpoints that arrest cell cycle progression and thereby provide time for repair and recovery This has led to the ... for CDK activa-tion and cell cycle progression Inhibiactiva-tion of Chk1 re-sults in premature activation of CDC25 phosphatases and CDK1/2, and progression through the cell cycle be-fore adequate ... performed Cell analysis Cell cycle analysis was performed by flow cytometry as de-scribed previously [10] For cell growth assays, cells were seeded at low density (500–1000 cells) in 96-well plates and

Ngày tải lên: 05/11/2020, 01:49

... (Figure 6B) Cell cycle distribution was assessed after staining fixed cells with PI and thereby cells in different phases of cell siRNA-transfected cell lines vs scrambled control and parental ... Lakes, NJ, USA), and expression of cell proliferation and cell death markers were compared with parental control cells Detection of the markers was followed by analysis of the cell cycle phases In ... tissues and two PDAC cell lines siRNA was used to knockdown HOXB7 mRNA in the cell lines and its consequences on apoptosis rate and cell proliferation were measured by flow cytometry and MTT

Ngày tải lên: 05/11/2020, 05:10

... tumorigenicity of RCC cells in vitro miR-99a induces G1-phase cell cycle arrest To investigate the role of miR-99a in cell cycle progres-sion, we restored miR-99a in RCC cells 786-O and OS-RC-2 cells were ... protein translation, metabolism, cell invasion, and cell cycle [26] Major downstream targets of mTOR are p70S6K and 4E-BP1, which is activated by mTOR and then dissociates from the eukaryotic translation ... RCC cell lines (786-O and OS-RC-2) and normal immortalized renal cell line (HK-2) (C) Expression of mTOR and p-mTOR protein were detected by western blotting assay in RCC cell lines (786-O and

Ngày tải lên: 05/11/2020, 07:50

... 2004 571.8'4 dc22 2004006931 Preface Cell Cycle Control and Dysregulation Protocols focuses on emerging methodologies for studying the cell cycle, kinases, and kinase inhibitors It addresses the ... manner We are confident that Cell Cycle Control and Dysregulation Protocols will facilitate and optimize the work of practical scientists involved in researching the cell cycle We greatly acknowledge ... Reviews and Protocols, Second Edition, edited by Paulina Balbas and Argelia Johnson, 2004 266 266 Genomics, Proteomics, and Clinical Bacteriology: Methods and Reviews, edited by Neil Woodford and

Ngày tải lên: 15/08/2022, 15:07

... p53 and cell-cycle DE-TARs likely reflects the role of p53 in cell-cycle control Whole genome tiling array experiments are demanding of RNA material This was particularly problematic for the cell-cycle ... to identify transcriptional activity depen-dent on cell cycle, pro- and anti-proliferative stimuli We decided to use cellular systems that give the clear-est results for each pathway and process, ... thetranscription of other ncRNAs as well Cell-cycle regulation resides at the core of tumor opment and progression A tightly controlled cellularmachinery defines the pace of proliferation and

Ngày tải lên: 02/11/2022, 08:47

... on the transcriptional or on the translational level, the system of combined transcription ⁄ translation was decoupled. The influence on the transcriptional level was investigated by in vitro transcription ... maximize the cell- free H-Ras expression by reducing Influence of CSPs on transcription and translation R. Hofweber et al. 4692 FEBS Journal 272 (2005) 4691–4702 ª 2005 FEBS translation ⁄ transcription ... proteins on transcription and translation studied in cell- free model systems Roland Hofweber 1 , Gudrun Horn 1 , Thomas Langmann 2 , Jochen Balbach 3 , Werner Kremer 1 , Gerd Schmitz 2 and Hans...

Ngày tải lên: 07/03/2014, 21:20

... mechanisms for controlling cell cycle progression (Morgan 1997; Zachariae and Nasmyth 1999). These studies and others demonstrated the universality of the cell Modeling Cell Cycle Control and Cancer with ... Nature 343:665–669 Modeling Cell Cycle Control and Cancer with pRB Tumor Suppressor 231 cycle, and strongly suggested that deregulation of the mechanisms controlling cell cycle progression could ... Orc1 and Mcm2–7) and DNA replication (e.g. Dhfr, Rnr, TK, TS and Polα). Many E2F target genes encode products in- volved in cell cycle progression (e.g. cyclins A and E, pRB, p107, E2F1–3) and apoptosis...

Ngày tải lên: 25/10/2013, 21:20

... most cell cycle responses. While each single pocket protein can mediate cell cycle arrest upon cell- cell contact, pRb is the critical mediator of cell cycle arrest upon growth factor deprivation and ... roles in controlling cell cycle exit upon growth-inhibiting signals. Only ablation of all pocket proteins fully alleviated a cell cycle arrest upon serum withdrawal, cell- cell contact inhi- bition, ... suppression and cell mortality. Cell 85:27–37 Serrano M, Lin AW, Mila EM, Beach D, Lowe SW (1997) Oncogenic ras provokes prema- ture cell senescence associated with accumulation of p53 and p16 Ink4a . Cell...

Ngày tải lên: 25/10/2013, 21:20

... during the cell cycle [13], upon exit and entry to the cell cycle both genes are controlled, at least in part, transcriptionally. Cdt1 and geminin are highly expressed in proliferating cells in ... upon cell cycle exit. To this effect, HFF cells were deprived of serum f or 48 h to induce cell cycle exit. Serum was th en r e-added a nd samples taken as cells progressed into the cell cycle. ... in G0 cells and induced upon cell cycle re-entry [23] and to be under the transcriptional c ontrol of E2F [24– 26]. We show here that, similar to Cdc6, Cdt1 mRNA levels are r educed in G0 and r...

Ngày tải lên: 07/03/2014, 16:20