cell cycle regulation and transcription

... cell cycle progression 1.1.2.1 RB/E2F/DP-mediated transcriptional regulation of cell cycle progression The E2F family of transcription factors integrates cellular signals and coordinates cell ... T cells of TRIP-Br2 wildtype and mutant mice (lymph nodes) .241 Figure 46 B220+ B cells and CD11c+ dendritic cells of TRIP-Br2 wildtype and mutant mice (spleen) 242 Figure 47 B220+ B cells and ... of the cell cycle and cancer 25 1.1.1 The general schema of cell cycle progression: simplifying complexity 25 1.1.2 The control of cell cycle progression 27 1.1.2.1 RB/E2F/DP-mediated transcriptional

Ngày tải lên: 13/09/2015, 20:18

... level, play a central role in cell cycle regulation Cell differentiation converts dividing cells into non-dividing cells and also determines cell fate In many cases, cell differentiation occurs ... CDKB1;1 and XTH genes These results strongly suggest that OBP4 is a negative regulator of cell cycle progression and cell growth These findings increase our understanding of the transcriptional regulation ... and cell expansion by regulating key cell cycle genes and cell expansion factors (Fig. 8) Figure 5 OBP4 regulates the expression of core cell cycle genes and cell expansion factors (A) Using qRT-PCR

Ngày tải lên: 19/11/2022, 11:49

... and mRNA during the Cell Cycle 126 Subcellular Localization 127 Activity at Cell Cycle Checkpoints 129 Interactions with Cell Cycle Proteins 130 Transcription of Cell Cycle Genes 131 Conclusion ... Inhibitors 33 Cytokine Regulation of Cell Cycle Progression 34 Links Between the Ras/Raf/MEK/ERK Pathway and Cell Cycle Proteins 34 Cytokine Regulation of Apoptosis and Cell Death 34 Apoptotic ... Hypoxia and Cell Cycle 143 Rachel A Freiberg, Susannah L Green and Amato J Giaccia Introduction 143 Cell Cycle and Check Points 144 Hypoxia-Induced Arrest 146 Mechanisms Underlying Cell Cycle

Ngày tải lên: 10/04/2014, 11:13

... cells more susceptible to radiation-induced DNA damage and, 3) downregulates total Chk1 and Cyclin B abrogating the radiation-induced G2/M checkpoint and allowing cells to re-enter the cell cycle ... Loratadine on In Vitro Cell Cycle Progression HT29 cells treated with loratadine (75μM) for 24 hours Loratadine was then washed off and cells were irradiated (8 Gy) Cell cycle progression was ... loratadine-treated cells clearly dominated the cell cycle delay and radiation with loratadine did not cause additional cell cycle delays As shown in Figure 5B, the percent of cells in G2 did not

Ngày tải lên: 09/08/2014, 10:20

... six different FLS cell lines with eight per treat-ment group Cell cycle analysis and evaluation of cell death For cell cycle analysis FLS were harvested after 4, 8, 16, 24 and 48 hours after ... showed a strong accumulation of intracellular CML com-pared with cells receiving Co-BSA (Figure 1b) Cell proliferation, cell viability, cell cycle and evaluation of cell death To evaluate whether ... Percentage of cells in the subG1 and G1 phases of the cell cycle Incubation of fibroblast-like synovial cells (FLS) with AGE-BSA increased the percentage of cells in the subG1 and G1 phases

Ngày tải lên: 09/08/2014, 14:22

... of cell cycle and cell differentiation In a developmental context, the cell cycle and cell differentiation are tightly coordinated both spatially and temporally Adequate crosstalk between cell ... 1999), including cell fate regulation (Macaluso et al., 2006), senescence (Funayama and Ishikawa, 2007), and apoptosis (Harbour and Dean, 2000b) Its dual function in cell cycle and cell differentiation ... coordinate cell cycle and cell differentiation, and several experiments have been conducted to test this hypothesis However the elaborate relationship between cell cycle and differentiation in multicellular

Ngày tải lên: 12/09/2015, 09:28

... the mechanism of cell growth inhibition Cell proliferation was analyzed by MTT assay, and the cell cycle and apoptosis were assessed by flow cytometry And we transfected the cells with the GFP-RFP-LC3 ... AccessBicyclol induces cell cycle arrest and autophagy in HepG2 human hepatocellular carcinoma cells through the PI3K/AKT and Ras/Raf/MEK/ERK pathways Yu Wang, Hao Nie, Xin Zhao, Yong Qin*and Xingguo Gong* ... inhibited cell cycle progression at G1 phase and induced autophagy in HepG2 cells, which implied that the significant decrease in cell proliferation was mainly induced by autophagy and inhibition of cell

Ngày tải lên: 20/09/2020, 18:01

... follicular cell lines, FTC133, FTC236, ML1 and TT2609, and the C643 and HTh7 ATC cell lines also had midrange IC50 values (2.9-3.2μM and 3.1-3.2 μM, respectively) HTh83 ATC cells and FTC238 FTC cells ... increased the proportion of cells in subG1 peak and induced cell cycle arrest in thyroid carcinoma cells To investigate the effects of sorafenib on cell cycle distri-bution and on cell death-associated ... of cells in subG1 for all cell lines, and different influences on cell cycle progression depending on the cell line So-rafenib induced a larger proportion of cells of papillary and anaplastic cell

Ngày tải lên: 30/09/2020, 11:14

... MM cells and inhibit STAT3 phosphorylation in U266 and MM1.S cells Next, we used western blotting to explore intracellular signaling proteins associated with cell survival in MM cells The cells ... cell proliferation in vitro and in vivo, and to elucidate the mechanisms of action of CaM antagonists, we have revealed two cellular and molecular mechanisms: induction of cell cycle arrest and ... naphtha-lenesulfonamide derivatives W-7 and W-13, selective and cell-permeable CaM antagonists, on proliferation, cell cycle progression, and apoptosis in human MM cell lines Furthermore, we demonstrated

Ngày tải lên: 14/10/2020, 13:10

... unrestricted use, distribution, and Trang 2DNA damage activates cell cycle checkpoints that arrest cell cycle progression and thereby provide time for repair and recovery This has led to the ... for CDK activa-tion and cell cycle progression Inhibiactiva-tion of Chk1 re-sults in premature activation of CDC25 phosphatases and CDK1/2, and progression through the cell cycle be-fore adequate ... performed Cell analysis Cell cycle analysis was performed by flow cytometry as de-scribed previously [10] For cell growth assays, cells were seeded at low density (500–1000 cells) in 96-well plates and

Ngày tải lên: 05/11/2020, 01:49

... (Figure 6B) Cell cycle distribution was assessed after staining fixed cells with PI and thereby cells in different phases of cell siRNA-transfected cell lines vs scrambled control and parental ... Lakes, NJ, USA), and expression of cell proliferation and cell death markers were compared with parental control cells Detection of the markers was followed by analysis of the cell cycle phases In ... tissues and two PDAC cell lines siRNA was used to knockdown HOXB7 mRNA in the cell lines and its consequences on apoptosis rate and cell proliferation were measured by flow cytometry and MTT

Ngày tải lên: 05/11/2020, 05:10

... tumorigenicity of RCC cells in vitro miR-99a induces G1-phase cell cycle arrest To investigate the role of miR-99a in cell cycle progres-sion, we restored miR-99a in RCC cells 786-O and OS-RC-2 cells were ... expres-sion of p-p70S6K and p-4E-BP1 were downregulated in Figure 3 MiR-99a induces G1-phase cell cycle arrest (A) Cell cycle analysis of transfected 786-O cells by FACS Cells which were transfected ... RCC cell lines (786-O and OS-RC-2) and normal immortalized renal cell line (HK-2) (C) Expression of mTOR and p-mTOR protein were detected by western blotting assay in RCC cell lines (786-O and

Ngày tải lên: 05/11/2020, 07:50

... Cell Cycle Control and Dysregulation Protocols focuses on emerging methodologies for studying the cell cycle, kinases, and kinase inhibitors Itaddresses the issue of gene expression in vivo and ... Dean, D C (2000) Rb function in cell-cycle regulation and apoptosis Nat Cell Biol 2, E65-E67. 14 Harbour, J W and Dean, D C (2000) The Rb/E2F pathway: expanding roles and emerging paradigms Genes ... kinases and centrosome lans Prog Cell Cycle Res 3, 221–232. 55 Ke, Y W., Dou, Z., Zhang, J., and Yao, X B (2003) Function and regulation of Aurora/Ipl1p kinase family in cell division Cell Res

Ngày tải lên: 15/08/2022, 15:07

... thetranscription of other ncRNAs as well Cell-cycle regulation resides at the core of tumor opment and progression A tightly controlled cellularmachinery defines the pace of proliferation and ... p53 and cell-cycle DE-TARs likely reflects the role of p53 in cell-cycle control Whole genome tiling array experiments are demanding of RNA material This was particularly problematic for the cell-cycle ... to identify transcriptional activity depen-dent on cell cycle, pro- and anti-proliferative stimuli We decided to use cellular systems that give the clear-est results for each pathway and process,

Ngày tải lên: 02/11/2022, 08:47

... most cell cycle responses. While each single pocket protein can mediate cell cycle arrest upon cell- cell contact, pRb is the critical mediator of cell cycle arrest upon growth factor deprivation and ... (42) P. Kaldis: Cell Cycle Regulation DOI 10.1007/002/Published online: 24 February 2006 © Springer-Verlag Berlin Heidelberg 2006 The Retinoblastoma Gene Family in Cell Cycle Regulation and Suppression ... suppression and cell mortality. Cell 85:27–37 Serrano M, Lin AW, Mila EM, Beach D, Lowe SW (1997) Oncogenic ras provokes prema- ture cell senescence associated with accumulation of p53 and p16 Ink4a . Cell...

Ngày tải lên: 25/10/2013, 21:20

... Regulated During the Cell Cycle by a CDE ⁄ CHR Tandem Element and is Downregulated by p53 but not by p63 or p73. Cell Cycle 6, 853–862. 37 Yoshida K (2005) Cell- cycle- dependent regulation of the human and mouse ... p53. Cell cycle arrest can be medi- ated by p53 downregulating the transcription of central cell cycle regulators such as cyclin B, Cks1, Cdc2 and Cdc25C. CDE ⁄ CHR-dependent cell cycle- gene transcription ... encoded by cyclin B1 and cyclin B2 genes is Keywords cell cycle; cell cycle genes homology region (CHR); cell cycle- dependent element (CDE); DREAM complex; E2F Correspondence K. Engeland, Molecular...

Ngày tải lên: 16/02/2014, 09:20

... completion of the cell division cycle is significantly extended compared with continually fed cells. Thus, starvation not only blocks cell cycle progression, but causes cells to exit the cycle and enter ... of the regulation of the cell cycle and its relation to cancer, which culminated in the 2001 Nobel Prize being awarded to Leland Hartwell, Tim Hunt, and Paul Nurse. In the investigations of cell cycle ... restriction point of the cell cycle. Cell Cycle 1:103–110 Buchkovich K, Duffy LA, Harlow E (1989) The retinoblastoma protein is phosphorylated during specific phases of the cell cycle. Cell 58:1097–1105 Burdon...

Ngày tải lên: 07/10/2012, 16:51

... the cell cycle process affected: initiation and maintenance of meiosis, transcriptional and translational fac- tors, cellular signaling, cytoplasmic and apoptotic factors and prophase I regulation. 2 Cell ... pregnancy loss and birth defects, it is critical to dissect out mei- otic cell cycle checkpoints and proper chromosome segregation (Hassold and Hunt 2001). However, understanding cell cycle regulation ... kinase, but male and female Cell Cycle Regulation in Mammalian Germ Cells 347 Table 1 (continued) Gene Protein Name Effect on germ cell development Refs. B. Transcriptional and Translational...

Ngày tải lên: 25/10/2013, 21:20

... Nature 343:665–669 Modeling Cell Cycle Control and Cancer with pRB Tumor Suppressor 231 cycle, and strongly suggested that deregulation of the mechanisms controlling cell cycle progression could ... mechanisms for controlling cell cycle progression (Morgan 1997; Zachariae and Nasmyth 1999). These studies and others demonstrated the universality of the cell Modeling Cell Cycle Control and Cancer with ... Orc1 and Mcm2–7) and DNA replication (e.g. Dhfr, Rnr, TK, TS and Polα). Many E2F target genes encode products in- volved in cell cycle progression (e.g. cyclins A and E, pRB, p107, E2F1–3) and apoptosis...

Ngày tải lên: 25/10/2013, 21:20

... REVIEW ARTICLE Cell biology, regulation and inhibition of b-secretase (BACE-1) Clare E. Hunt and Anthony J. Turner Proteolysis Research Group, Institute of Molecular and Cellular Biology, ... 10001–10006. 110 Andrau D, Dumanchin-Njock C, Ayral E, Vizzavona J, Farzan M, Boisbrun M, Fulcrand P, Hernandez JF, Martinez J, Lefranc-Jullien S et al. (2003) BACE1- and BACE2-expressing human cells: ... with isophthalamide derivatives as the P 2 –P 3 ligands showed good selectivity between BACE-1 and BACE-2, nanomolar potency in vitro and in cell- based studies, and a significant reduction in Ab40 levels...

Ngày tải lên: 07/03/2014, 00:20