cell and tissue culture and plant regeneration

... design of culture systems Keywords:  3D printing, Prototyping, Plant tissue culture, Micropropagation, Light quality, LED lighting system, Culture vessel design Background Plant tissue culture ... is the aseptic culture of cells, tissues, organs or whole plants under controlled nutritional and environmental conditions, allowing the growth and development of the cells or tissues to be manipulated ... al Plant Methods (2017) 13:6 multiplied to produce several thousand plants in a relatively short time period and little space on a year round basis Despite the importance of plant tissue culture

Ngày tải lên: 19/11/2022, 11:41

... differentiated plant cells; a wound response Cell Culture  Maintenance or cultivation of cells in vitro, including culture of single cells In cell cultures, the cells are no longer organized into tissues Cell ... bifurcatum, 133 Pitcher plants, 141 Plant cell/tissue culture, 1–2 Plant growth regulators, 32–36 Plant modification and improvement, in recent past, 7–8 Plant protoplasts/cells, Plant quality, 46 ... Explant Culture  The maintenance or growth of an explant in culture Feeder Layer  A layer of cells (usually lethally irradiated for animal cell culture) upon which is cultured a fastidious cell

Ngày tải lên: 04/10/2023, 15:52

... of the explanted tissues in preparation for drying; secondly tissue dehydration; thirdly tissue freezing; and finally tissue recovery involving thawing and plantlet production Three tissue dehydration ... advancement in tissue culture include the heterogeneous response of diverse coconut explanted tissues, the slow growth of these explanted tissues in vitro, and their further lack of vigour when planted ... CO2 enrichment system and photoautotrophic culture (Samosir and Adkins 2014) 2000 1962 Advent of the most commonly used basal medium in plant tissue culture (Murashige and Skoog 1962) Genetic

Ngày tải lên: 17/12/2017, 15:22

... Programmed cell death in animal development and disease Cell 2011, 147:742–58 Gunawardena AHLAN: Programmed cell death and tissue remodelling in plants J Exp Bot 2008, 59:445–51 Noodén LD (Ed): Plant Cell ... Jones AM: Programmed cell death in development and defense Plant Physiol 2001, 125:94–7 Lord CEN, Gunawardena AHLAN: Programmed cell death in C elegans, mammals and plants Eur J Cell Biol 2012, 91:603–13 ... in LPCD cells in comparison with EPCD cells Large aggregates form in EPCD cells and are readily apparent in LPCD cells TVS increase notably in EPCD cells but are less frequent in LPCD cells Approximately

Ngày tải lên: 26/05/2020, 23:29

... (30.78 and 27.41 cm), (11.52 and 10.49) and in plant density was D1 at 5th MAP (31.85 and 28.24 cm)(11.88 and 11.04) and shooting stage (62.61 and 60.18cm) and 18.39 and 17.32) of plant and ratoon ... registered in D3 (triple plants per pit) in plant (10.50 and 16.26) and ratoon crop (9.31 and 15.54) Both in plant and ratoon crop, the highest number of leaves was registered in single plant per pit with ... optimized in tissue culture banana especially cv Ney Poovan (AB) for adopting the high density planting system with two or more plants per pit and effectively utilization of land and solar energy

Ngày tải lên: 14/10/2020, 18:20

... Queensland in 2016 ARC Centre of Excellence in Plant Cell Walls, Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation Abstract The nutritional and health ... determined and quantified, with the binding capacities following the order of Cellulose>Cellulosearabinoxylan>Cellulose-xyloglucan>Cellulose-pectin>Apple cell walls, for the binding of (+/-)catechin and ... the chemical organization and local microstructure of cell wall components, and the charged nature of cell wall polymers) and extrinsic factors (e.g pH, temperature and salt) are major factors

Ngày tải lên: 11/07/2021, 16:37

... the way a mammalian cell and yeast cells coordinate cell growth and cell-cycle progression Ian Conlon and Martin Raff Address: MRC Laboratory for Molecular Cell Biology and Cell Biology Unit, ... eukaryotic cells in culture, including yeast cells and mammalian cells, the mean cell size remains constant over time, even though individual cells vary in size at division [10]. Thus, cells that ... if large and small cells grow and progress through the cell cycle at the same rates (after Brooks [10]). The initial division is unequal and produces one cell of 10 mass units and one cell of

Ngày tải lên: 06/08/2014, 18:20

... including 78 vascular plants and 7 moss and lichen species and it was higher than in reclaimed sites where the total number was 77 including 70 vascular plant species and 7 moss and lichen species ... thickness of humus horizon, and energy trapped in soil organic carbon and plant biomass in the developing ecosystem 2–3 times and nitrogen accumulation 5 times. Keywords: sand mining; reclamation; ... community and ecosystem level process Supported by the Polish Ministry of Scientific Research and Information Technology, Grant No. 3 P06S 039 25. Soil and plant communities development and ecological

Ngày tải lên: 07/08/2014, 10:22

... thus, it is a key variable for understanding water and gas exchange processes in trees, both at plant and stand scales. g L is regulated by biological and environmental variables, but the relative ... 3.2. Effects of atmospheric evaporative demand, leaf water status and plant hydraulic conductance The effects of atmospheric evaporative demand and plant hydraulic factors on g L were analysed ... conductance and plant car- bon gain [12]. However, Niinemets [28] recently analyzed a huge amount of data on Picea abies (L.) Karst. and Pinus sylvestris L., covering 126 stands of various height and

Ngày tải lên: 08/08/2014, 01:21

... collagenase and DNase, and passed through a cell strainer to produce single-cell suspensions The cells were pelleted by centrifugation at 500g for minutes at 4uC, and then red blood cells were ... Materials and Methods, and mononuclear cells were isolated from the lungs Cells were stained with fluorescein isothiocyanate (FITC)–anti-CD11c and rhodamine (Rhod)-anti-CD11b, and CD11c+b+ cells ... 071101-02 and VA Merit Review and Career Scientist Award to S.S.M and the Florida Biomedical Research Foundation Bankhead-Coley Award and Mabel and Ellsworth Simmons Professorship to S.S.M., and by

Ngày tải lên: 08/08/2014, 21:20

... significantly elevated levels of T cell related cytokines (IL-2, IL-4, IL-13 and IL-17) and stromal cell and macrophage related cytokines (EGF, bFGF, IL-1 and IL-15) when compared with synovial ... leucocytes and stro- mal cells (for review [54]). For example, it induces IL-6 and IL- 8 production by fibroblasts [55] and stimulates macrophage IL-1 and TNF-α production [56]. IL-15 stimulated T cells ... immune and stromal processes that are present soon after the clinical onset of rheumatoid arthritis (RA) by assessing a panel of T cell, macrophage, and stromal cell related cytokines and chemokines

Ngày tải lên: 09/08/2014, 06:22

... Introduction Cell line CHO dhfrCell line Sf9 Cell line Schneider-2 Cell lines COS 1/COS Cell line NIH3T3 Cell line HeLa Cell line J558L Cell line Vero Myeloma cell lines Hybridomas Cell line MRC-5 Cell ... of cells and their first subculture Use of haemocytometer5 for the calculation cell densities of Cell culture §ubculture of suspension cells Subculture of adherent cells Rejection of failed cell ... 4.3 xvi CULTURE CELL AND TISSUE Angela Martin National Cell and Tissue Culture Centre, Bioscience Ireland, School of Biological Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland Section:...

Ngày tải lên: 10/04/2014, 11:12

... INTRODUCTION TO CELL AND TISSUE CULTURE: Theory and Technique Jennie P Mather and Penelope E Roberts Page iii Introduction to Cell and Tissue Culture Theory and Technique Jennie P Mather and Penelope ... to cell and tissue culture : theory and technique / Jennie P Mather and Penelope E Roberts p cm.¡ª (Introductory cell and molecular biology techniques) Includes bibliographical references and ... mammalian cell in vitro The Practice and Theory of Tissue Culture This book is meant to serve as an introduction to cell culture both for students who have little or no experience of cell culture and...

Ngày tải lên: 11/04/2014, 09:48

... treatment involved 30-60 explants and was repeated twice Culture media and conditions Organ cultures Explants were cultured on modified GresshoffDoy (GD) medium (Gresshoff and Doy, 1972), BTM (Chalupa, ... embryogenesis and plant regeneration in Picea, Quercus, Betula, Tilia, Robinia, Fagus, and Aesculus Commun Inst For Cech 15, 133-148 V (1987b) European hardwoods In: Cell and Tissue Culture in Forestry ... proliferation Biol Plant 30, 414-421 318 Chalupa V (1990c) Plant regeneration by somatic embryogenesis from cultured immature embryos of oak (Quercus robur L) and linden (Tilia cordata Mill) Plant Cell Rep...

Ngày tải lên: 08/08/2014, 19:21

... remaining stem cells for tissue regeneration and repair? In conclusion, MSCs derived from adult tissue present an exciting progenitor cell source for applications of tissue engineering and regenerative ... stem cells in order to identify core ‘stem’ genes and to examine supportive tissue to understand what genes and pathways are involved not only in stem -cell differentiation, but also in stem -cell ... engineering and regeneration [134] Mesenchymal stem cells versus embryonic stem cells Embryonic stem (ES) cells are derived from the inner cell mass of the embryonic blastocyst These cells can...

Ngày tải lên: 09/08/2014, 01:21

... isolates WE54, LCMV Cl13, and Traub efficiently target antigen presenting cells (dendritic cells) in the spleen and perturb their ability to present antigen to T cells and B cells, resulting in a ... cytotoxicity, and limited tropism Able to withstand ultracentrifugation, LCMV glycoproteins yield MLV and HIV vector titers similar to the widely used amphotropic and VSV-G envelopes [1-3] Stable cell ... investigate the expression of a- and b-DG in the liver at pre- and postnatal time points and document the in vivo tissue tropisms of these LCMV pseudotypes in neonatal and adult mice We hypothesized...

Ngày tải lên: 14/08/2014, 19:22

... catalase–peroxidases and APXs have been outlined [15], and a dendrogram of 29 lignin and manganese peroxidases have been presented [16] The present study should contribute to our understanding of the ... Blosum 30 protein–weight matrix; helix and strand gap penalty, 4; and loop gap penalty, Finally, areas of extensive gaps (i.e longer than 10 amino acid positions and present in more than 90% of sequences) ... and Chlamydomonas reinhardtii) and all thylakoid APXs from higher plants analysed here An important observation is the occurrence of two different APXs from the same organism (e.g thylakoid and...

Ngày tải lên: 19/02/2014, 16:20

... negative than Yw (cell) (–0.244 MPa), and water will move from the turgid cell to the solution As water leaves the cell, the cell volume decreases As the cell volume decreases, cell Yp and Yw decrease ... loss and the threat of dehydration A major difference between plant and animal cells that affects virtually all aspects of their relation with water is the existence in plants of the cell wall Cell ... water loss by the cell and consequent increased concentration of cell solutes Contrast this with the volume changes of a cell lacking a wall Measurements of cell water potential and cell volume (see...

Ngày tải lên: 20/02/2014, 01:20