cd9 cd63 and cd81 are dispensable for hiv 1 budding

... http://www.retrovirology.com/content/6 /1/ 64 relative infectivity A 1. 5 1. 0 0.5 0.0 vector scramble CD63 relative transmission B CD9 CD 81 CD9/ 63 CD9/ 81 CD9/ 63/ 81 shRNA 6.0 * 5.0 4.0 3.0 2.0 1. 0 0.0 vector scramble CD63 CD9 CD 81 CD9/ 63 ... regulators of HIV- 1 spread through their presence in HIV- 1- producing cells Despite the enrichment of CD63, CD9 and CD 81 at HIV1 budding sites, their knockdown did not affect the efficiency of HIV- 1 release ... significant Results CD9, CD63 and CD 81 are dispensable for HIV- 1 budding Because tetraspanins are known to enhance or repress the function of peripheral and integral membrane proteins and because of...

Ngày tải lên: 12/08/2014, 23:21

... immunodeficiency virus type gp120 J Virol 2000, 74 :19 48 -19 60 Page 11 of 11 68 Moscicki AB: Vaginal microbicides: where are we and where are we going? J Infect Chemother 2008, 14 :337-3 41 69 Elechiguerra JL, ... UC-7 81 as a virucidal agent AIDS 19 98, 12 :11 29 -11 38 McGowan I: Microbicides: a new frontier in HIV prevention Biologicals 2006, 34:2 41- 255 Fowler MG, Melnick SL, Mathieson BJ: Women and HIV Epidemiology ... transmission of HIV J Acquir Immune Defic Syndr Hum Retrovirol 19 96, 11 : 211 -2 21 13 Zussman A, Lara L, Lara HH, Bentwich Z, Borkow G: Blocking of cell-free and cell-associated HIV- 1 transmission...

Ngày tải lên: 11/08/2014, 00:22

... HIV- 1 infection and is suppressed by the viral Vif protein Nature 2002, 418 :646-650 Page 11 of 12 (page number not for citation purposes) Retrovirology 2004, 1: 27 10 11 12 13 14 15 16 17 18 19 ... http://www.retrovirology.com/content /1/ 1/27 APOBEC3G As before, HeLa cells were co-transfected with a 1: 1 ratio of pNL-A1 and pHIV-APO3G plasmid DNAs Cells were grown on cover slips and fixed 24 hr later either with formaldehyde ... H, Landau NR: Species-Specific Exclusion of APOBEC3G from HIV- 1 Virions by Vif Cell 2003, 11 4: 21- 31 Marin M, Rose KM, Kozak SL, Kabat D: HIV- 1 Vif protein binds the editing enzyme APOBEC3G and...

Ngày tải lên: 13/08/2014, 13:20

... novel selective ligands BQ -12 3 and [Ala1,3 ,11 ,15 ]ET -1 Biochem Biophys Res Commun 18 2, 14 4 15 0 40 Saeki T, Ihara M, Fukuroda T, Yamagiwa M & Yano M (19 91) [Ala1,3 ,11 ,15 ]Endothelin -1 analogs with ETB ... constant KD for binding of ETBcHx to bET -1 as 6.2 ± 1. 7 · 10 )9 (Fig 7) Similar assays with the C-terminal-truncated derivatives ETB1 31 and ETB93 revealed KD values of (2.7 ± 1. 9) · 10 )8 and (1. 7 ± ... kDa NTD T1 C1 T2 E1 T3 C2 T4 E2 T5 C3 T6 E3 T7 CTD C-terminal tag ETBDT7 ETBcHx ETBStrep ETB1 31 ETB168 ETB203 ETB306 ETB132 ETB204 ETB307 ETB93 M1–S443 Q2–S443 E27–S443 E27–C1 31 E27–P168 E27–V203...

Ngày tải lên: 16/03/2014, 10:20

... of glucagon fibrillation J Mol Biol 387, 14 7 16 1 64 Wu CS & Yang JT (19 80) Helical conformation of glucagon in surfactant solutions Biochemistry 19 , 211 7– 212 2 65 Goldsbury C, Frey P, Olivieri V, ... Blundell T (19 75) X-ray analysis of glucagon and its relationship to receptor binding Nature 257, 7 51 757 FEBS Journal 277 (2 010 ) 45 91 46 01 ª 2 010 The Authors Journal compilation ª 2 010 FEBS 46 01 ... of a beta 1- 40: implications for the search for a beta fibril formation inhibitors J Struct Biol 13 0, 217 –2 31 25 Kumar S & Udgaonkar JB (2009) Structurally distinct amyloid protofibrils form on separate...

Ngày tải lên: 23/03/2014, 03:20

... Lundkvist for fruitful discussion and Prof Antti Vaheri for general support This work was supported by the research grants RFA 915 and 202 012 from the Academy of Finland 20 References 21 10 11 Worobey ... Division) http://www.virologyj.com/content/2 /1/ 12 12 13 14 15 Competing interests The author(s) declare that they have no competing interests 16 17 Authors' contributions AngP participated in ... products of RT-PCR with primers RECF738 and RECR855 Lines and show negative controls M, molecular weight marker; bands of 14 7 and 11 0 bp are indicated by arrows and the N protein of Tula hantavirus...

Ngày tải lên: 18/06/2014, 22:20

... 1. 0 01 1.0 01 to 1. 002 < 0.0 01 Plasma DNA for each increase of 500 GE/ml) 1. 756 1. 314 to 2.347 < 0.0 01 1.359 1. 125 to 2.350 < 0. 01 6-h lactate (for each increase of mmol/l) 1. 348 0. 912 to 1. 6 31 NS ... NS 1. 115 0.765 to 1. 530 NS Age (for each increase of one year) 1. 0 31 0.899 to 1. 0 41 NS 1. 376 0.865 to 1. 240 NS Admission glucose (for each increase of 10 mg/dl) 1. 019 0.833 to 1. 282 NS 1. 012 ... 37 (33 to 41) NS Plasma DNA (GE/ml) 2,400 (1, 370 to 3,550) 4 ,15 0 (3,460 to 5 ,18 0) 0.0 01 Basal lactate (mmol/l) 10 .8 (9 .1 to 12 .2) 11 .5 (10 .3 to 13 .1) NS 5.5 (4.9 to 6.6) 6.9 (5.8 to 8.4) NS 52...

Ngày tải lên: 13/08/2014, 20:21

... THE FORMATION AND CONCEPTS OF DUTY-FREE BUSINESS 1. 1 Introduce NoiBai Airport Services Join-Stock Company (NASCO), Duty-free Department (DFD) and Duty-free shops 1. 1 .1 NASCO 1. 1.2 DFD 1. 2 Formation ... world and in Vietnam 1. 2 .1 History and development of duty-free business in the world 1. 2.2 The formation of duty-free business in Vietnam 1. 3 Concepts of duty-free business 1. 3 .1 Definition 1. 3.2 ... & Accessories Total Item Can Can Item Item 71 6 81 2% 357 15 9 11 0 34% 443 669 1% 24 469 0 .1% 14 8 27 533 6% 464 785 10 0% (Report of NASCO – 2009) Cigarette was one of the most kinds of goods sold...

Ngày tải lên: 18/04/2013, 14:39

... Your System Functional 18 1 Chapter Doing: Making the Best Action Choices 19 1 Chapter 10 Getting Projects Under Control 211 Part 3: The Power of the Key Principles Chapter 11 The Power of the Collection ... Books India (P) Ltd, 11 Community Centre, Panchsheel Park, New Delhi - 11 0 017 , India Penguin Books (N.Z.) Ltd, Cnr Rosedale and Airborne Roads, Albany, Auckland, New Zealand Penguin Books {South ... life and personal life and get a lot more done with less effort And you can make frontend decision-making about all the "stuff" you collect and create standard operating procedure for living and...

Ngày tải lên: 14/09/2013, 20:01

... transcription 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 890 G A Muller and K Engeland ¨ transcription initiation site coincides with induction of cdc2 expression Mol Cell Biol 15 , 69 01 6 913 Zwicker ... RK, Cobrinik D, Lee MH & Dyson N (19 97) pRB and p107 ⁄ p130 are required for the regulated expression of different sets of E2F responsive genes Genes Dev 11 , 14 47 14 63 89 Korenjak M, Taylor-Harding ... CDE and CHR elements A report implied p53 and p21WAF1 ⁄ CIP1 in this downregulation by employing p53-positive or p53-negative cell lines [69] For the Plk1 gene, coding for Polo-like kinase 1, ...

Ngày tải lên: 16/02/2014, 09:20

... Rac1 is regulated by JNK A 6His-myc-Ub + - + myc-Rac1L 61 - + + R147 R16 R96 + + + Cobalt extraction IB : Rac IB : Rac IB : actin Rac1L 61 MG132 (20 µM) - Rac1L61R147 + - Rac1L61R16 + - + Rac1L61R96 ... HA–Rac1L 61, HA– Rac1bWT, HA–Rac1bL 61 and 6His–myc–Ub as indicated Proteasomal degradation of Rac1L 61 and Rac1b (right panel) Proteasomal degradation was assessed by determining Rac1L 61, Rac1bWT and ... B myc-Rac1 WT L 61 L61R16 L61R147 input myc-Rac1 WT L 61 L61R16 L61R147 GST PAK GST PAK GST PAK GST PAK IB : myc GTPase activity, and failure to interact with RhoGDI; as a result, Rac1b has been...

Ngày tải lên: 18/02/2014, 16:20

... HTLV1 LTR Ets-responsive region by transcription factors Ets1 and Sp1 EMBO J 12 , 11 69– 11 78 43 Schaefer, T.S., Sanders, L.K & Nathans, D (19 95) Cooperative transcriptional activity of Jun and ... Oct -1, Ets -1, and Sp1/Sp3 As fgl2 expression is tightly controlled [49], the interaction of transcription factors Sp1/Sp3 with the fgl2 promoter form a core to recruit both Oct -1 and Ets -1 to form ... nuclear extracts for 45 before adding DNA probe Anti-Sp1, Sp3, Stat3, Oct -1, Oct-2, Ets1/2, and PU .1 Ig are from Santa Cruz Biotechnology Inc (Santa Cruz, CA, USA) For competition, 10 0 · cold oligo...

Ngày tải lên: 20/02/2014, 11:20

... http://www.rbej.com/content/3 /1/ 19 22 23 References 10 11 12 13 14 15 16 17 18 19 20 21 Luck MR: The gonadal extracellular matrix Oxford Reviews in Reproductive Biology 19 94, 16 :33-85 Rodgers RJ, van ... 1. 00 10 .00 10 0.00 % Round cells % Pyknotic cells (b) 0. 01 0 .10 1. 00 10 .00 10 0.00 % Round cells (c) Steroid secretion 15 00 10 00 500 0. 01 0 .10 1. 00 http://www.rbej.com/content/3 /1/ 19 10 .00 10 0.00 % ... *** * 20000 ** 10 000 10 0.0 18 .0 14 .0 (b) 10 .0 % Pyknotic cells 30000 Number of cells http://www.rbej.com/content/3 /1/ 19 * 7.5 5.0 2.5 0.0 0.0 * ** 10 0.0 *** *** 10 00 10 0.0 18 .0 14 .0 0.0 (d) 400...

Ngày tải lên: 05/03/2014, 17:20

... Basilica 10 0 Brass of Titus, showing the Colosseum 10 5 Region I Monti, Device of 10 6 Santa Francesca Romana 11 1 San Giovanni in Laterano 11 6 Piazza Colonna 11 9 Piazza di San Giovanni in Laterano 12 6 ... Augustus 16 9 Forum of Trajan 17 1 Ruins of Hadrian's Villa at Tivoli 18 0 Palazzo del Quirinale 18 5 Region III Colonna, Device of 19 0 Arch of Titus 19 1 Roma Immortalis, Vol 1, by Francis Marion Crawford ... Republic and the Empire fought for power and conquest and got both by force, endurance and hardness of character, so, in her second life, others fought for Rome, and courted her, and coveted her, and...

Ngày tải lên: 08/03/2014, 12:20

... the old United States dollar, which consisted of 25 and 8 -10 grains of gold, or 412 and 5 -10 grains of silver To allow the laws of supply and demand free play, Congress should authorize at once ... arbitrary, and therefore unjust? A man wishes to obtain certain lands, wagons, &c., and applies to you for money to buy them with: you can lend the money for six per cent interest, and no more; ... exchange, all of which are liable to be presented for discount For example: Suppose Peter sells John $10 0 worth of pork at six months' credit, and takes a bill at six months for it; and that John sells...

Ngày tải lên: 29/03/2014, 08:20

... and 10 lM) and NIM 811 (10 lM) caused significant inhibition of mitochondrial swelling **P < 0. 01 versus control; ns, not significant by two-way ANOVA C Isolated mitochondria CsA (1 and 10 lm) and ... 5 417 R, rotor F45-30 -11 , Eppendorf AG, Hamburg, Germany (all centrifugation carried out using same centrifuge and rotor types) The supernatant was collected and centrifuged at 11 000 g for 10 ... removed, and the pellet was resuspended in 10 volumes of extraction buffer and centrifuged at 600 g for Finally, the supernatant was centrifuged at 11 000 g for 10 The supernatant was removed, and...

Ngày tải lên: 30/03/2014, 08:20

... gts1D pACGTS1[N-C]/gts1D pACGTS1[DKN]/gts1D pACGTS1[C53Y]/gts1D tdh1D tdh2D tdh3D pACTDH1/tdh1D pACTDH1pr.TDH2/tdh1D pACTDH1pr.TDH3/tdh1D 5.03 5 .12 5 .10 5.08 5.03 5. 01 4.58 3.32 4.95 3.40 2.45 ± ... in gts1D, the recombinant plasmids carrying GTS1[C53Y] and GTS1[DKN] in centromere-based plasmids, named pACGTS1[DKN]/gts1D and pACGTS1[DKN]/gts1D were transformed into gts1D, and the transformants ... oligonucleotides for 5¢ and 3¢ primers, and for TDH1, Ó FEBS 2002 Gts1p–GAPDH1 interaction in metabolic oscillations (Eur J Biochem 269) 3563 and for TDH2, and and (Table 1) for TDH3, respectively,...

Ngày tải lên: 31/03/2014, 21:21