candidate for a potential pan hpv vaccine

... of primers for zebrafish  ef1α (forward primer: CTGGAG GCCAGCTCAAACAT-3' and reverse primer: 5'-ATCAAGAAGAGTAGTACCGCTAGCATTAC-3')  MC1R (forward primer: GACCACG GCCTCCTGGATGT-3 and reverse ... 5-GTTGCAGAAGGGGCTGGTGG-3)  MITFa (forward primer: 5'-TGTACAGC AATCATGCTCTTCC-3' and reverse primer: 5'-GTCCCCAGCTCCTTAATTCTGTC-3')  Tyrosinase (forward primer: CGCAGATGA ACAATGGCTC-3 and reverse ... defect [Figure 5]a Data statistical analysis by GraphPad software (GraphPad Software, Inc., La Jolla, CA 92037 USA) gave the concentration-response curves for lethality and developmental defects [Figure

Ngày tải lên: 12/12/2017, 06:31

... (metachromasia) Normal (compared with host adjacent cartilage) 3 Surface regularity a Thickness of cartilage b Integration of donor with host adjacent cartilage a Total smooth area of the reparative cartilage ... attractive cell source for cartilage regenerative medicine because they can be har-vested in a minimally invasive manner, are easily isolated and expanded, and have multipotentiality that includes chondro-genesis ... Trang 1Open AccessVol 10 No 4 Research article Local adherent technique for transplanting mesenchymal stem cells as a potential treatment of cartilage defect Hideyuki Koga1, Masayuki Shimaya1,

Ngày tải lên: 09/08/2014, 10:23

... SL and TS evaluated and interpreted SNP microarray and aCGH data All authors read and approved the final manuscript for publication Acknowledgments We thank the heterotaxy patients and families ... we have developed a network biology analysis appropriate for evaluation of candidates relative to potential interactions with known genetic pathways for heterotaxy, LR patterning, and ciliopathies ... quality at each aligned base position which serves as the basis for variant calling Variants are reported based on a configurable formula using the following additional parameters: depth of coverage,

Ngày tải lên: 09/08/2014, 23:20

... Zakaria, Abu Bakar Salleh2, Raja Noor Zaliha Raja Abdul Rahman3, Mohd Basyaruddin Abdul Rahman1 Abstract Background: Cosmeceuticals are cosmetic-pharmaceutical hybrids intended to enhance health ... Trang 1R E S E A R C H Open AccessA potential tocopherol acetate loaded palm oil esters-in-water nanoemulsions for nanocosmeceuticals Brian Sheng Xian Teo1, Mahiran Basri1*, Mohd Rezuwan Shah ... vitamin E In addition, formulations F1 and F2 showed phase separation after the third week of analysis There-after, particle sizing analysis was ceased for F1 and F2 The stability of the formulations

Ngày tải lên: 11/08/2014, 00:22

... Cassandra.Roschel@gmail.com; Muhammad Mukhtar - mukhtar.muhammad@gmail.com; Alagarsamy Srinivasan - alagarsamy.srinivasan@gmail.com; Mohammad Rafi - Mohammad.Rafi@jefferson.edu; Roger J Pomerantz - RPomeran@its.jnj.com; ... Philadelphia, PA 19107, USA and 6 Tibotec Inc 1020 Stony Hill Road, Suite 300, Yardley, PA 19067, USA Email: Edward A Acheampong - eacheamp@yahoo.com; Cassandra Roschel - Cassandra.Roschel@gmail.com; ... Rockford, IL) and the mem-branes were probed with specific monoclonal antibodies against GFAP at a concentration of 1:1000, mouse anti-Caspase 3 antibody at a concentration of 1:1000 as pri-mary antibodies

Ngày tải lên: 12/08/2014, 04:20

... z-axis Image analysis was performed using Advantage Windows 4.2 (GE Medical Systems) and AccuLite Trang 5(AccuImage Diagnostics Corporation, South San Francisco,CA, USA) Statistical analysis Data were ... required an alternative vascular access due to only minimal Qa via the dual-lumen catheter Neither hemorheologically relevant aspects nor CO and CVP correlated with the Qa achievable via the femoral ... [International Organization for Standardization] 9001) The principles of laboratory animal care were followed with respect to the guidelines of the Euro-pean and German societies of laboratory animal

Ngày tải lên: 13/08/2014, 03:20

... for the APOPEC3G-UBA2 fusion was 5'-ATCGTACTCGAAGCTTCTAACTCAGGAG-GAAGTTGGCAG-3'; and the 3' primer for the APOPEC3G-UBA2* fusion was 5'-ATCGTACTCGAAGCT-TCTAACTCAGagcGAAGTTGGCAG-3' Purified PCR ... 5'-ATCCAAGACG-GAATTCGTTTTCCTGATTCTGGAG-3' The UBA2 gene fragment was amplified from plasmid pcDNA3.1-HHR23A by PCR The 5' primer used was 5'- ATCCAAGACGGAATTCACGCCGCAGGAGAAA-GAAGCTATAG-3'; the 3' primer for the APOPEC3G-UBA2 ... 5'-GCGCGCGCGCCTCGAGACCATGAAGCCT-CACTT-3'; The 3' primers used for the construction of the E plasmid was 5'-ATCCAAGACGGAATTCCTA-GAACTCGTTTTCCTGATTCTGGAG-3' and the 3' primer used for the U and M plasmid was 5'-ATCCAAGACG-GAATTCGTTTTCCTGATTCTGGAG-3'

Ngày tải lên: 13/08/2014, 05:21

... samples were obtained at baseline (day of randomization) and daily through to study day 7 A central laboratory (Covance Central Laboratory Services, Indianapolis, IN, USA) performed all assays ... was a randomized, Company, Indianapolis, IN, USA) in adult patients with severe sepsis ENHANCE was an open-label, single-arm, clinical trial of DrotAA All investigative sites obtained approval ... 28-day mortality RR reduction (DrotAA versus placebo) for each potential biomarker at baseline Illustration of 28-day mortality RR reduction (DrotAA versus placebo) for each potential biomarker

Ngày tải lên: 13/08/2014, 08:21

... Fishers Lane, Bethesda, MD 20892-9403, USA, 2 Laboratory of Pediatric and Respiratory Viral Diseases, Center for Biologics Evaluation and Research, Food and Drug Administration, Bldg 29A, 8800 ... permeabilization of the fixed cells allowed a comparison of intracellular and cell surface expression of the chimeric CD4 proteins Each protein was transported to the plasma membrane and was availa-ble ... DNA transfection, the func-tionality of the CD4 proteins was initially evaluated in HeLa cells by a syncytia-forming assay Infection of trans-fected HeLa cells with a vaccinia virus recombinant

Ngày tải lên: 13/08/2014, 09:21

... probe 5'FAM-ACGCCGTCTTCCTCCATCTCATA GC-TAMRA3' Thermal cycler parameters included one cycle at 94°C for 2 min, and 45 cycles involving denaturation at 94°C for 10 s annealing at 53°C for 30 s and ... recommendation of the manufacturer(Dharmacon Research, USA) [19] The following sequences were successfully made: siRNA-EGFR sense GGAGCUGCCCAUGAGAAAUdTdT-3' and antisense 5'-AUUUCUCAUG GGCAGCUCCdTdT-3' ... receptordouble stranded RNAsmall interference RNAnon small cell lung cancer Abstract Lung cancer has emerged as a leading cause of cancer death in the world Non-small cell lung cancer (NSCLC) accounts for

Ngày tải lên: 14/08/2014, 19:22

... TEWL and decreased skin capacitance in areas adjacent to the application sites, as far as approximately 0.75cm away (Patil, Singh, & Maibach, 1995) It appears that SLS acts in a time-and-dose ... completed each segment of my project and were instrumental to the technical aspects of this project For Dr Ravi Sahu, Dr Mohammed Al-Hassani, Dr Sarita Sehra, Dr Simarna Kaur, Qiaofang Yi, Davina A Lewis, ... suprabasal keratinocytes in a complex biochemical cascade involving phosphatases and proteases into the active form filaggrin (FLG) (Koch, et al., 2000) FLG has a high affinity for keratins and

Ngày tải lên: 24/08/2014, 12:37

... Replication Protein Binding Trans-acitivtor1 Trang 18SAGA Spt-Ada-Gcn5-Acetyltransferase Trang 19ABSTRACT The TRIP-Br proteins are a novel family of transcriptional regulators that function at E2F1-responsive ... Medicine of National University of Singapore, especially Prof Bay Boon Huat, Ms Stacy Tan, Ms Geetha Sreedhara Warrier and Ms Malika Raguraman for their kind helps during the last four years in NUS ... mutant protein lead to Rubinstein-Taybi Syndrome (RTS), a human developmental disorder comprised by mental retardation, an unusual facial appearance, broad thumbs, and broad big toes (Murata et

Ngày tải lên: 12/09/2015, 08:19

... demonstrated that mpa and pafA mutants are severely attenuated in a mouse model of infection (Darwin et al., 2005) Mpa is an ATPase that forms hexamers like the Clp ATPase Meanwhile, pafA, despite ... Clp ATPase belongs to the AAA+ superfamily of ATPases AAA+ proteins are generally modulated by a group of otherwise unrelated proteins termed adaptor proteins An adaptor protein serves as an accessory ... adds 11 amino acid tag (AANDENYALAA) to the incomplete protein Proteins tagged with the SsrA peptide are targeted for degradation The C-terminal Ala-Ala residues are critical for SsrA recognition

Ngày tải lên: 26/11/2015, 22:38

... Cornell University, Ithaca, NY Yamada, T., Sekiguchi, Y., Imachi, H., Kamagata, Y., Ohashi, A., and Harada, H., (2005) Diversity, Localization, and Physiological Properties of Filamentous Microbes ... THAI NGUYEN UNIVERSITY UNIVERSITY OF AGRICULTURAL AND FORESTRY DAM HA LUONG THANH BIOMETHANE POTENTIAL TEST FOR RAPID EVALUATION OF ANAEROBIC DIGESTION OF SEWAGE SLUDGE FROM MULTIPLE MATERIALS ... hydrate solution 24 Table Characteristics of inoculum and materials 28 Table Bio-methane potential of substrates 29 Table Weighted average methane potential per kg of substrate

Ngày tải lên: 05/06/2018, 14:40

... is “a residual solid from sewage Trang 6plants treating domestic and urban waste water and from other sewage plants treating waste water of a composition similar to domestic and urban waste water”2.1.1 ... contain oil, grease, vegetable materials, paper, faecal materials, sanitary and medical waste, kitchen waste Treatment process such as activated sludge process, or rotating biological contactors results ... organic waste, and stabilized residue for a long time Over the last two decades, a great deal of the literature has been published on feasible applications of AD for solid waste and wastewater

Ngày tải lên: 28/03/2019, 10:54

... fiberglass tank with biofiltration and foam fractionation operated in by-pass Tanks were filled with approximately 150 l of filtered natural seawater and covered with transparent acrylic glass to avoid ... to marine and estuarine fish and crustaceans are limited Trang 23Classical LC50 tests were performed with blue crab (Callinectes sapidus) and Atlantic menhaden (Brevoortia tyrannus) (Richardson ... fisheries cannot cover the increasing demand for aquatic animal protein for human consumption anymore (FAO, 2009a) This lack of aquatic animal protein represents one of the greatest challenges

Ngày tải lên: 27/09/2019, 10:30

... data for Bafia 27 Table 4-7: Site reference and climate data for Yaoundé 28 Table 4-8: Site reference and climate data for Douala 30 Table 4-9: Site reference and climate data for Bertoua ... MWh Megawatt Hour - NASA National Aeronautics and Space Administration - NGO Non-governmental Organization - NRCan Natural Resources Canada - OECD Organisation for Economic Co-operation and Development ... reference and climate data for Garoua 23 Table 4-4: Site reference and climate data for Ngaoundéré 24 Table 4-5: Site reference and climate data for Bamenda 25 Table 4-6: Site reference and climate

Ngày tải lên: 06/01/2020, 09:53

... dry reforming (CO2 reforming) and partial oxidation of methane Dry reforming of methane (DRM) (1) has drawn attention because this process takes advantage of the available CO2 in natural gas reservoirs ... NiO-based catalysts such as coke formation and sintering at high reaction temperatures, many diverse researches from using new carriers to supporting catalyst by alkali, alkaline earth metals and ... supports, leading to a reduction of coke formation and an increase of CO2 adsorption [45] For example, adding a basic Lewis promoter such as alkali metal oxides (Na2O, K2O), alkaline earth (CaO, MgO)

Ngày tải lên: 12/01/2020, 00:26

... validatinghydrocarbon associated anomaly[41] The last example depicts the effect of fractures on S-wave propagation Both radial and transverse component data are displayed Traces recorded at different angles are ... characterization and monitoring 1 Alberta, Canada [27] 2 Manitou Lake, Canada [45] 3 Ross Lake heavy oilfield in Saskatchewan, Canada [46] 4 Faja Petrolı´fera del Orinoco, Venezuela [47] Reservoir monitoring ... resources (shale’s oil/gas, tight sand reservoirs) 1 British Colombia, Canada [10] 2 Marcellus Shale, Pennsylvania, US [44] 3 Shaunavon Tight Oil Reservoir, Canada [11] Heavy oil characterization and

Ngày tải lên: 12/01/2020, 00:35

... Trang 1ORIGINAL ARTICLEEl-Salam canal is a potential project reusing the Nile Delta drainage water for Sinai desert agriculture: Microbial and chemical water quality Nabil A Hegazi b,* a National ... Delta drainage water is annually discharged into the Mediterranean Sea El-Salam (peace) canal, having a mixture of such drainage water and the Nile water (1:1 ratio), crosses the Suez canal eastward ... Spectrochim Acta B 2006;61:180–4. [34] Tavares TM, Carvalho FM Avaliac¸a˜o de exposic¸a˜o de populac¸a˜es humanas a metais pesados no ambiente: exemplos do Recoˆncavo Baiano’ Quı´mica Nova 1992;15:147–54

Ngày tải lên: 13/01/2020, 14:28