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... tctgttctgttggccctttt mStARqFwd ttgggcatactcaacaacca mCycD2Fwd ctgtgcatttacaccgacaac mStARqRev acttcgtccccgttctcc mCycD2Rev cactaccagttcccactccag mSCCqFwd aagtatggccccatttacagg mCox-1Fwd cctctttccaggagctcaca ... cagttctgcagcagtgtcatc mHpgdsRev aattcattgaacatccgctctt mCYP19Fwd cctcgggctacgtggatg mLPgdsFwd ggctcctggacactacacct mCYP19Rev gagagcttgccaggcgttaaa mLPgdsRev atagttggcctccaccactg mEP2Fwd tgctccttgcctttcacaat ... cctctttccaggagctcaca mSCCqRev tggggtccacgatgtaaact mCox-1Rev tcgatgtcaccgtacagctc mDP1Fwd cccagtcaggctcagactaca mCox-2Fwd gctcttccgagctgtgct mDP1Rev aagtttaaaggctccatagtacgc mCox-2Rev cggttttgacatggattgg

Ngày tải lên: 20/06/2014, 07:20

... evaluating the effect of curcumin in modulating the altered dopaminergic receptors, CREB and phospholipase C in the cerebral cortex and cerebellum of STZ induced diabetic rats Radioreceptor binding ... binding parameters were determined using Scatchard analysis [32] The specific binding was deter-mined by subtracting non-specific binding from the total The binding parameters, maximal binding ... mRNAs to the CT values of the internal control β-actin in the same sam-ples (ΔCT = CTTarget - CT β-actin) It was further normal-ized with the control (ΔΔCT = ΔCT - CTControl) The fold change in expression

Ngày tải lên: 10/08/2014, 05:21

... manufacture's specification (Invitrogen, Carlsbad, CA, USA) The control pCMV (Clontech, CA, USA) was co-transfected to monitor the transfection effi-ciency After 24 hr, the cells were then co-treated ... monocytic cell line were obtained from the American Type Culture Collection (Cryosite, Lane Cove NSW, Australia) Dulbecco's modified Eagle medium (DMEM), penicillin, streptomycin, and fetal bovine ... statistically significant in the inhibi-tory effect of Bee venom on the LPS-induced, and in the inhibitory effect of melittin on the SNP-induced NF-κB in THP-1 cells by 10 μM of SP600125 JNK inhibitor suppressed

Ngày tải lên: 11/08/2014, 08:22

... 90%, respectively In addition, AtMYB60 and VvMYB60 disclosed a distinctive PHEEG signature, encompassing the two highly conserved glutamic acid residues, located in the loop connecting the R2 and ... proteins and other closely related proteins from subgroup 1: a distinctive PHEEG sig-nature in the MYB domain, located in the loop connecting the R2 and R3 repeats, and the lack of the first (CtM1) ... Lucio Conti1,2, Eleonora Cominelli1,5, Chiara Tonelli1and Patricio Arce-Johnson3* Abstract Background: Under drought, plants accumulate the signaling hormone abscisic acid (ABA), which induces

Ngày tải lên: 11/08/2014, 11:21

... 5'-GTGGGGCGCCCCAGGCACCA-3', reverse primer: 5'-CTTCCTTAATGTCACGCACGATTTC-3') were used as control for RNA integrity PCR was done using an Icycler Thermal cycler (Bio-Rad, Hercules, CA, USA) under conditions ... entry (CCE) Ca2+ entry via SOC (SOCE) in the membrane caused by ER depletion is the dominated component of CCE [16] Ca2+ influx via SOC appears to be a determinant in maintaining a sustained increase ... progressive disease characterized by a sustained increase in pulmonary arterial pressure and vascular remodeling A few molecular mechanisms such as prostacyclin, nitric oxide (NO)/cyclic guanosine monophosphate

Ngày tải lên: 12/08/2014, 14:20

... subsequences containing only the 1st and 3rd codon letters (13) are compared The black dots in the RCMs indicate amino acids that are within 6Å of each other in the protein structure The colored ... the end of a codon pattern indicates the presence (-P), in contrast to the non-presence (-N), of that particular codon code to determine a specific amino acid co-location in a concrete protein ... 81) This periodic FFE difference is not present in introns It is therefore a specific physico-chemical characteristic of coding sequences and might contribute to unambiguous definition of codon

Ngày tải lên: 13/08/2014, 23:20

... transfectant and introduced to cells for 1 hour Trang 22Table 1 DNA Oligonucleotides Primer name Sequence (5'→3') Sense ATACCGTCCCACCATCGGGC Antisense GAATTCCTAAGCAGTCACTTGATCCTTTT 30A CCCCTATCCTTTCCGCGTCCTTACTTCCCC ... catalyst is used to activate the side chain of a specific amino acid that then forms an intermediate This intermediate then acts as a nucleophile and attacks the side chain of an adjacent amino acid ... detected by two methods of zero-distance crosslinking Zero distance crosslinking is characterized by covalently binding of two molecules directly together without the aid of a linker arm (40) A catalyst

Ngày tải lên: 24/08/2014, 11:02

... :• Price/Performance a key decision factor in new accounts • Need to maintain and enhance competitive edge • Maintain Common Business Logic across Mainframe, UNIX and Windows • Deliver Scalable, ... for Connected and Disconnected data access  Disconnected data access liberates SQL application from a continual connection to a database  Enhance the performance of web and other applications ... Managed code solution reusing existing COBOL mainframe DB2 code in service based architecture • Match or exceed mainframe performance and scalability • Micro Focus Net Express with NET combined

Ngày tải lên: 23/10/2014, 20:11

... and the overall impact on the innovation process Some of the key findings within the report include: ● Telecoms firms are increasingly embracing Open Innovation The traditional R&D model in ... focused on Don’t know/ refining business processes refining business processes refining business processes Not applicable To what extent is innovation at your firm focused on refining existing ... perhaps, the process involves a readiness to accept that individuals, coming together from disparate corporate and non-corporate backgrounds, may now hold the key to the most successful brainstorming

Ngày tải lên: 06/12/2015, 23:01

... 5′-GCATCTTGGATTACACGGATACG; 5′GTGGGTTTTGGGTAGGCTC; VEGFA: 5′- CTTGCCTTGCTGCTCTACC; 5′CACACAGGATGGCTTGAAG; VEGF-C: 5′-ATGTGTGTCCGTCTACAGATGT; 5′GGAAGTGTGATTGGCAAAACTGA; Actin B: 5′-TCCTGTGGCATCCACGAAACT; ... Background Clear cell renal cell carcinoma (ccRCC) is the most common epithelial tumor of the kidney that occurs in adults It is characterized by malignant epithelial cells with clear cytoplasm and a compact-alveolar ... to the Creative Commons licence, and indicate if changes were made The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated

Ngày tải lên: 06/08/2020, 05:29

... Epithelial-mesenchymal transition, Urinary bladder neoplasms Background Bladder cancer (BC) is the most common carcinoma of the urinary tract The cancer is the sixth leading cancer in men and the ... TGCCACGTCTTCTGTCTGTC-3’), E-cadherin (forward: 5’-CGGGAATGCAGTTGAGGA TC-3’; reverse: 5’-AGGATGGTGTAAGCGATGGC-3’), Vimentin (forward: 5’-GACCTCTACGAGGAGGAGA T -3’; reverse: 5’-TTGTCAACATCCTGTCTGAA-3’) ... that CLASP2 is crucially implicated in the carcinogenesis and invasion of BC Few studies about the roles of Vimentin in bladder cancer had been published The role of Vimentin in predicting progression

Ngày tải lên: 20/09/2020, 01:22

... sequences were as follows: mouse growth hormone (GH): forward, 5′-CAGCCTGATGTT CGGCACCTCGGA-3′ and reverse, 5′-GCGGCGACAC TTCATGACCCGCA-3′; mouse IGF-1: forward, 5′-CT GGACCAGAGACCCTTTGC-3′ and ... photographed and counted using a dissecting microscope Administration of growth hormone and prolactin in mice Mice were intravenously injected with B16F10 cells (6 × 104) GH or prolactin (5 mg/kg each; R&D ... heat-inactivated FBS, 100 U/ml penicillin, and 0.1 mg/ml streptomycin (Invitrogen, Carlsbad, CA) Knockdown of GHR in the cells was achieved by transfecting the cells with a plas-mid vector carrying

Ngày tải lên: 20/09/2020, 18:35

... lung cancer [2] NSCLC is highly resistant to the existing cancer therapeutics and the great majority of NSCLC patients are diagnosed at advanced tumor stage Although the recent advances in clinical ... cause of cancer-related mor-talities worldwide [1] Histologically, NSCLC is consisted of three different subtypes: squamous cell carcinoma, adenocarcinoma, and large cell carcinoma, accounting for ... Giodini A, O'Connor DS, Plescia J, Padgett KM, Tognin S, Marchisio PC, Altieri DC Survivin exists in immunochemically distinct subcellular pools and is involved in spindle microtubule function

Ngày tải lên: 21/09/2020, 09:52

... C-terminal cleaved TCTP specifically associates with Apaf-1 of apoptosome in apoptosome-forming condition thereby inhibiting the amplification of caspase cascade Conclusions: TCTP protects the cancer cells ... etoposide-induced cell death by inhibiting the mitochondria-mediated apoptotic pathway Interaction of TCTP with Apaf-1 in apoptosome is involved in the molecular mechanism of TCTP-induced chemoresistance ... blotting in the presence or absence of TCTP in the reaction (C) TCTP-induced inhibition of caspase-9 and -3 activities confirmed by inhibitor assay The specific inhibitor of caspase-9 and -3, Ac-LEHD

Ngày tải lên: 05/11/2020, 01:32

... primers used for ChIP were as follows: 5′-CACGCGCCACAGCGATCTCACTGA-3′ (−472 to −449, sense) and 5′-AGATCCGATCTCCACAAGCACTCC-3′ (−284 to −261, antisense) The PCR product was separated in 1.5% agarose ... GGATTGTCCC NM_000833 GATGGTTGT ACTGTACCAAG NM_000834 TCCTTGTTCAG CATCAGTGT NM_000835 CAGCAAAGAA ATGGTTTCCT NM_000836 TCGGTTGA TAGCAGTAATGA NM_133445 AGACGGCAGTG GCTCACAG NM_138690 CACAGGTTGA AAACAAGAT ... exist in other cancer cells as reported in lung carcinoma cells [28] Conclusions In conclusion, this study provides insight into the role of the FOXO signaling pathway on growth inhibition by the

Ngày tải lên: 05/11/2020, 05:15

... significant increase in the percentage of cells containing pyknotic nuclei, compared to siScr– PC3 transfected cells (Figure 3C) These data further reinforce the notion that PCA3 modulates PCa cell ... androgen-responsive intronic ncRNAs have been described, indicating that intronic ncRNAs, such as PCA3 [31], may have control mechanisms that are com-mon to protein-coding transcripts, such as those involv-ing ... Interestingly, siScr-transfected LNCaP cells showed no significant increase in cell growth during the time-course evaluation, suggesting that the decreased cell growth after PCA3 knockdown in siPCA3-transfected

Ngày tải lên: 05/11/2020, 07:41

... you in? floor is your classroom? Trang 9Which grade is Phong in? Which class is Phong in? Where is Phong’s classroom?Trang 10Which grade is Thu in?Which class is Thu in? Where is Thu’s classroom? ... tâm tin học và dịch vụ khách hàng tỉnh Bắc Giang • Trung tâm tin học và dịch vụ khách hàng trực thuộc bưu điện Bắc Giang được thành lập với nhiệm vụ: Cung cấp các dịch vụ, bán hàng, sửa chữa ... báo cáo, ghi chép gây nhàm chán dẫn đến hiệu quả công việc giảm sút. • Cần có một chương trình để thay thế các công việc thủ công là một yêu cầu cấp thiết. Trang 28Cơ chế quản lý của hệ thống cũTrang

Ngày tải lên: 20/04/2021, 05:37

... results in a quenching of the intrinsic flavin fluorescence, suggesting that the flavin chromophore is shielded from the solvent in the protein–protein complex [19] From the fluorescence titration assays, ... quenching of flavin fluorescence, confirming that these two proteins not interact with hMS We have compared the electrostatic potentials for the surface of the CPR FMN domain (in the region of the ... of MS During primary turnover, the homocysteine-binding domain (dotted barrel) and the CH3-H4-folate binding-domain (black barrel) form discrete complexes with the cobalamin-binding domain (dark...

Ngày tải lên: 18/02/2014, 08:20

... CTAGATCTAAC-3¢ and 5¢-AGGCCCCGGGTCACCTC CTAGCTAGAATTC-3¢ for a1; and 5¢-AGGTGATC ATATGCTTCTAGAGAAGAGTGAAATA-3¢ and 5¢-AG AGGATCCTCAGCCCATTTGGAGGGCGG-3¢ for b1 In each case, the forward primer introduced ... MKIVCRCNDV, respectively The N-terminal aminoacid sequence of the a2 subunit corresponded to the underlined amino-acid sequence within MEIVRINEHPILD from the putative protein encoded by the predicted ORF ... another, as yet unknown, dyel-proDH is produced by this organism In the present study, we identified the gene encoding this other enzyme, expressed it in Escherichia coli, and examined the characteristics...

Ngày tải lên: 20/02/2014, 01:20

... maps of the E focardii c- T1 and c- T2 nanochromosomes Coding, noncoding regions, introns, and telomeres (C4 A4 ⁄ G4T4) are indicated in the key The coding sequences of the E focardii c- T1 and c- T2 ... centrosome function in the closed orthomitosis of the micronucleus We did not detect c- tubulin or microtubules in the macronucleus of E focardii, in contrast to reports that c- tubulin and microtubules ... GATA-transcription factor binding site in the c- T2 promoter, although other processes might also be involved In multicellular organisms, GATA-binding factors play critical roles in development, including...

Ngày tải lên: 07/03/2014, 04:20