but not cooking the books

... Scc2 or Scc4 They found that inactivating either protein not only reduced the amount of bound condensin but also eliminated cytological benchmarks of yeast chromosome condensation Thus, the authors ... of genes included the majority of the yeast tRNA genes, of which there are 274 distributed across the genome Both complexes were even found at some sites known as ETCs, where the general transcription ... (Haeusler et al [21]) These findings suggest that condensin, either through the ability to oligomerize or the ability of single complexes to bind multiple sites, brings together the dispersed tRNA...

Ngày tải lên: 14/08/2014, 21:20

... on the inventory in the A through G sections We went over to talk with the staff and asked about their progress They told us they started their counting in section D and figured they needed another ... journal entries with the notation ‘Jenny’s entry’ as the explanation for the transaction.” I asked, “Is there any other information about the purpose of the entry?” “No, that’s all there is,” she said ... not owned by them individually or by their company Burns did his best to convince the SEC investigator the options were being renewed and they should not have been written off at the end of the...

Ngày tải lên: 01/11/2014, 17:49

... determine whether the tyrosine phosphorylation status of tau also affects the binding of tau to Fyn-SH3, CHO cells were cotransfected with Fyn together with either wild-type or the YF mutant ... of the association of these proteins could represent a potential therapeutic strategy for the treatment of AD Here, we used GST fusion proteins of Fyn-SH2 and FynSH3 to further investigate the ... to interact, but to differing extents, with Fyn-SH3 This suggests that the results shown here are not artefactual, owing to incorrect folding of the tau mutants, at least not in the regions responsible...

Ngày tải lên: 14/02/2014, 14:20

... it is apparently not used for ubiquitin addition Therefore, the reason why Lys147 rather than Lys5 is the main acceptor site for ubiquitin in Rac1 remains to be elucidated Of note, however, is ... (R63) Overexposure allows the detection of the monoubiquitinated form of Rac1, in the presence of UbR48 (C) Ubiquitinated forms of Rac1L61 accumulate in the presence of the proteasomal inhibitor ... (Rac1b) The structure of Rac1b switch I, shown as a green line at the bottom, is clearly further away from the nucleotide (GTP, in red), explaining a decreased affinity for nucleotide in Rac1b The...

Ngày tải lên: 18/02/2014, 16:20

... addition, the fact that protein levels of COX I and COX Vb, but not mRNA levels, are increased in SOL and PV– ⁄ – TA suggest that these differences are the result of translational, rather than ... seen in SOL These findings further support the presence of distinct pathways regulating fiber specific isoforms and metabolic enzymes, on the one hand, and mitochondrial biogenesis, on the other Renewed ... we addressed the question of whether the profile of selected mitochondrial proteins corresponds to the one observed in either fast- or slow-twitch muscles Beforehand, we evaluated whether such differences...

Ngày tải lên: 19/02/2014, 07:20

... In other words, the crystal structure is encoded in the diffracted X-rays – the shape and symmetry of the cell define the directions of the diffracted beams, and the locations of all atoms in the ... a map, the parts existing in the structure, but not included in the model, should show up in the positive map contours, whereas the parts wrongly introduced into the model and absent in the true ... distinguished in the electron-density maps The higher the resolution, that is, the smaller the d spacing, the better, because there are more independent reflections available to define the structure The terms...

Ngày tải lên: 16/03/2014, 06:20

... With these characteristics, the maximum velocities in the direct (V8d, synthesis of ATP) and in the reverse (V8r, synthesis of ADP) directions were mathematically adjusted, using the MATHEMATICA-3.0 ... Inuence of the hypothetical enzyme (E8) recycling ATP, on the rate of synthesis of inosine Application of the theoretical model was performed with the MATHEMATICA-3.0 program, as described in the text ... hypothetical enzyme has been used to test, with the help of the mathematical model described in [3], whether different rates of synthesis of ATP would modify the intracellular pool of inosine The...

Ngày tải lên: 17/03/2014, 10:20

... subunit of the PtdIns-3 Kinase We are currently investigating whether ubiquitination of Ruk L and P85 could affect the association between them and the activity of PtdIns-3 kinase The use of ... anti-HA antibody (top panel) The positions of polyubiquitinated Ruk species [(Ub)n-Ruk] are indicated The arrowheads mark the locations of the unmodified forms of Ruk L, M, S The membrane was reprobed ... to the Ruk L isoform, has been recently reported [12,18] These findings and the results presented above prompted us to investigate whether all Ruk isoforms can interact with Cbl To assess these...

Ngày tải lên: 17/03/2014, 23:20

... that differ from one another in subtle but distinct ways, such as variations in the mobility of the aromatic Tyr ring There is a growing number of similar observations from other peptide systems ... that the exponential growth phase extends through the apparent lag all the way to the beginning of the experiment [23] (Fig 2C), which means that the observed lag time depends inversely on the ... spectrum of peptides, further substantiating the structural differences between the two types of fibril [56] The two fibril types also differ in terms of the mechanisms leading to their formation, as...

Ngày tải lên: 23/03/2014, 03:20

... latch may be the last event to occur being triggered not by direct disruption of the interface but as a consequence of the Kd being rather large At low pH, the helical latch could be the rate limiting ... time in the absence (––) or the presence (- - -) of lM G4–6 calcium induced release of the helix latch may occur with other rearrangements between domains caused either by the occupancy by other ... with the poor accessibility of the tryptophan suggested by the position of the maximum wavelength of the fluorescence (336 nm at pH 5.7 and 342 at pH 6.8 in EGTA) The results obtained using the...

Ngày tải lên: 23/03/2014, 21:20

... perfectly match their theoretical values, assuming formation of all seven disulfide bonds in the sPLA2 molecule Influence of the mutations on the secondary structure and overall conformation of the AtnI2 ... NLYQF…, of each recombinant chimera confirmed the specific cleavage of the fusion protein during activation just after the last R of the fusion peptide The molecular masses of chimeric proteins, ... (w/v) agarose gel The larger DNA fragment was purified from the gel, cleaved with BamHI and HindIII, and the restriction fragment (399 bp) inserted into the expression vector as above The nucleotide...

Ngày tải lên: 31/03/2014, 08:20

... and the heavy chain of HLA-I; ii) that the disruption of the first transmembrane domain of HPV16 E5 modifies the subcellular distribution of the protein; and iii) that the disruption of the first ... SDS-PAGE migration attributable to the different hydrophobicity of the proteins To analyze the differential involvement of the each of the three E5 transmembrane domains in the interaction between ... Immunoblot showing the expression levels of the codon-optimised E5 gene (left panel) and of the viral E5 gene (right panel) Note the differences in the immunoreactivity signals despite the higher amount...

Ngày tải lên: 18/06/2014, 18:20

... receptor, cluster with each other but not with the viral encoded chemokines targeting the same receptor There is one exception, vCCL2, which on the dendrogram in Figure lies in the middle of a cluster ... results was found using the two vector constructs (data not shown) The low potency of mXCL1 on the mXCR1 in the IP3 assay could also be related to the cell-line used, but again this explanation ... activation of the murine but not the human lymphotactin receptor Antagonistic activity on XCR1 and mXCR1 We next tested whether vCCL2 was able to act as an antagonist on the murine and the human...

Ngày tải lên: 20/06/2014, 01:20

... adjective, these word can be eirther attributive (that is , usually preceding but occasionally following a noun) Ex: door The child, laughing happily, ran out of the He seened extremely pleasant The ... words in underline are phrases in examples below: The sun rises in the east Peter sat on a wall The girl with red hair is an artist The Phrase There are five commonly occurring types of phrase ... to come next year The Phrase A preposition phrase: begins with a preposition and can act as a noun, an adjective or can adverb Ex: On the table by the car , over the rainbow The Phrase A number...

Ngày tải lên: 13/07/2014, 23:26

... retinal-pigmented epithelium in the maxillary sinus Not only is the deposition of pigment clearly visible (higher magnification insets) but also the induction of proliferation of underlying tissues and the change ... hyperplastic brain phenotypes were observed at a low penetrance in Ptdsr-mutant mice (less then 4.5% of homozygotes), but these not resemble to any extent the brain-overgrowth phenotypes of caspase- ... pathways in the worm [45] But the loss-of-function phenotype of psr-1 mutants and the complementation phenotypes in overexpressing transgenic worms shown by Wang et al [45] are rather weak as...

Ngày tải lên: 06/08/2014, 18:21

... nifedipine is consistent with the hypothesis that VEGF signalling is impaired in SSc Our results also not support the implication of the sVEGFR-1 in the VEGF dysregulation, but more experimental evidence ... suggests that these drugs improve endothelium injury A decrease of sVCAM-1 by nifedipine was previously reported in SSc [24] The improvement of all these markers and the correlation between the values ... [17] The data reported herein confirm these results with a different design of the study (midterm effects) and extend the known beneficial effects of these drugs to a vascular marker (sVCAM) The...

Ngày tải lên: 09/08/2014, 01:23

... glycosaminoglycans (S-GAGs) are available, these assays not distinguish between synthesis and degradation of the proteoglycans [19] Furthermore, they not distinguish MMPmediated degradation that ... understanding of the molecular events leading to cartilage destruction Many alternative hypotheses and conditions, including but not limited to the following, need to be investigated: (a) whether the aggrecanases ... according to the manufacturer's instructions Extraction of the cartilage explants The amount of S-GAG in the cartilage explants after termination of the culture was determined by extraction of the proteins...

Ngày tải lên: 09/08/2014, 10:23

... coordinating the incoming triphosphate In the process, Arg72 donates hydrogen bonds to the a-phosphate and the ε-amino group of Lys65 donates hydrogen bonds to the g-phosphate These events lead to the ... http://www.virologyj.com/content/8/1/33 K65R and L74V and the rare occurrence of K65R+L74V on the same HIV genome [6,27] may be related to the observed attenuation of the virus in the presence of these mutations [5,7,12,15] ... HIV-infected patients The preferential selection and persistence of one mutation relative to another, however, is not well understood Specifically, the rare combinations of mutations have not been analyzed...

Ngày tải lên: 11/08/2014, 21:21

... between the Rar1silenced plants and other resistant controls but revealed significant differences with the susceptible control, Katahdin These results show that the RB-mediated resistance was not ... sporangial count, than the K41 control, the transgenic but not silenced Rar1-RNAi and Sgt1-RNAi lines and the Rar1silenced plants (Figure 6) These results are consistent with those from the conventional ... process until the end of the image selection The data report details the total number of counted particles, representing the total number of fluorescing sporangia on the leaf surface As the Phytophthora...

Ngày tải lên: 12/08/2014, 05:20