biochemical and molecular analysis of bone cells

... planning of the project. XH was the leader of the project. SY and WZ amplified the complete genome and analyzed the genome profile. QS and FH went on the sequence alignment. All authors read and approved ... Yang 1 and Xiuguo Hua* 1 Address: 1 School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai 200240, PR China and 2 School of Medical Science and Laboratory ... Sapovirus SaKaeo-15/Thailand Table 2: Summary of sapovirus strains and representative strains for Lagovirus, Vesivirus, and Norovirus genera and NB-like viruses used in sequence analysis Strains Genus/genogroup...

Ngày tải lên: 12/08/2014, 04:21

... better clarify the occurrence of LOXs and their influence on hazelnut seed quality, we carried out the biochemical and molecular characterization of the main LOX isoforms expressed during seed ... products of the hazelnut LOX were compared with the reaction products of soybean LOX type I and authentic standards of 9- and 13-HODE. As shown in Fig. 4, 9-HODE is the main product of the hazelnut ... correlate LOX activity and the production of C 6 and C 9 aldehydes deriving from the activity of other enzymes of the LOX pathway, we carried out a gas- chromatographic analysis of the head space...

Ngày tải lên: 21/02/2014, 00:20

... Trang 1Biochemical and molecular characterization of a laccaseShicheng Chen, Wei Ge and John A Buswell Department of Biology and the Centre for International Services ... activity of 13.5 UÆmg protein)1 Corresponding pH optima and specific activities for syringaldazine and 2,6-DMP were pH 5.6 and 3.4UÆmg protein)1and pH 4.6 and 2.8 UÆmg protein)1, respectively In standard ... apparent Kmvalue of 0.03 mMand a Vmax of 16.4ÆU mg protein)1 Corresponding values for syring-aldazine and 2,6-DMP under optimal conditions were 0.01 mM and 4.9 UÆmg protein)1, and 0.57 mM and 5.6 UÆmg...

Ngày tải lên: 07/03/2014, 15:20

... addresses *Division of Metrology for Quality Life, Korea Research Institute of Standards and Science, Daejeon, Republic of Korea Division of Molecular Biology, Department of Zoology, Sri Venkateswara ... (*YpCM, y2828), purification of the protein, investigation of the properties of the enzyme, and the crystal structure analysis of the protein. Results and Discussion Annotation of CMs in M. tuberculosis ... alignment that helix 3 of 90-MtCM is a shorter version of helix 3 of EcCM, TtCM and PfCM lacking two of the binding site residues (Fig. 4C) corre- sponding to Ser84 and Gln88 of EcCM as discussed below. During...

Ngày tải lên: 17/03/2014, 17:20

... a molecular mass of 14 kDa and a specific activity of 400 UÆmg)1 in the presence of 1 mm sodium taur-odeoxycholate and 4 mm CaCl2with phosphatidylcholine as substrate The N-terminal sequence of ... 50% of its activity in the absence of NaTDC The maximal PLA2specific activity of 400 UÆmg)1was measured at pH 9.5 and at 37C in the presence of 1 mm NaTDC and 4 mm CaCl2 Pure ChPLA2 lost  80% of ... flow rate of 1 mLÆmin)1. Molecular mass markers were used to esti-mate the molecular masses of eluted pro-teins Right panel: SDS ⁄ PAGE (15%) Lane 1: molecular mass markers Lane 2: 10 lg of fractions...

Ngày tải lên: 30/03/2014, 01:20

... Trang 1Open AccessResearch Biochemical and virological analysis of the 18-residue C-terminal tail of HIV-1 integrase Address: 1 Department of Cancer Immunology and AIDS, Dana-Farber Cancer ... dimer-of-dimers architecture for the active IN tetramer [29,30] and the high affinity binding mode of the com-mon lentiviral integration cofactor LEDGFp75 [31] An SH3 fold comprised of five  strands ... l of LEDGFp75 was added, and the reac-tions were allowed to proceed at 37°C for 1 h The final concentrations of IN and LEDGFp75 were both 0.8 M Reactions stopped by the addition of EDTA and...

Ngày tải lên: 12/08/2014, 23:22

... PROTEIN AND MOLECULAR ANALYSIS OF YEAST FACTOR Trang 2LIVE-TRACKING VIRE2 PROTEIN AND MOLECULAR ANALYSIS OF YEAST FACTOR PHILOSOPHY DEPARTMENT OF BIOLOGICAL SCIENCES NATIONAL UNIVERSITY OF SINGAPORE ... Bacterial cells from 4 ml of overnight culture were collected by centrifugation The cells were washed once with TES and with 75 μl of 3M NaCl, 62.5 μl of proteinase K (5 mg/ml) and 62.5 μl of 10% ... assay of VirE2 in yeast cells 68 Figure 3.22 GFP11 does not perturb the function of VirE2 in AMT of plants 71 Figure 3.23 Study of putative nuclear localization signals of VirE2 in AMT of N...

Ngày tải lên: 09/09/2015, 11:19

... expression of CD56 isoform transcripts in tissues and leukocytes 89 4.4.6 Trout CD56 expression in T and myeloid cells contrast to IgM + B cells and thrombocytes 90 4.4.7 Up-regulation of trout ... processing and presentation via MHC class II by professional antigen presenting cells (macrophages, dendritic cells and B cells) During recent years, genes encoding MHC class I and II, TCR and their ... maturation of B cells into plasma and memory B cells and activation of CD8+ T cells and macrophage Activated CD4+ T cells also secrete many different cytokines that regulate and propagate the immune responses...

Ngày tải lên: 27/03/2020, 07:23

... Isolation, Biochemical and Molecular Characterization of Endophytic Bacteria from Tomato (Lycopersicon esculentum Mill.) B Sai Sushma*, B Vidya Sagar, S Triveni and G Uma Devi Department of Plant ... Materials and Methods Isolation of endophytic bacteria Sample collection endophytic bacteria and isolation of Healthy tomato plant samples were collected and separated into root, shoot and leaf ... rinse and plated on TSA to check the efficacy of surface sterilization A total of24 isolates obtained in this manner were maintained on TSA slants and stored at 4°C Biochemical characterization of...

Ngày tải lên: 28/04/2021, 01:50

... syndrome The clinical and biochemical characteristics were obtained and mutation testing of the IDS gene was done on the probands and their female relatives Results: The mean age of the patients was ... Dr Paul Hwu of the National Taiwan University Hospital for doing the urine GAGs and enzyme assays of the patients; to the staff of Biochemical Genetics la-boratory of the Institute of Human Genetics-NIH ... in the collection and interpretation of data and wrote the paper; DC was the one who did the mutation analysis of all patients and female relatives; MA helped in the collection of data; MB helped...

Ngày tải lên: 19/11/2022, 11:46

... Delbrueck Center for Molecular Medicine, Berlin, Germany 3 Actelion Pharmaceuticals Ltd, Allschwil, Switzerland 4 Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, ... terms of charge and is positioned at the tip of a loop and is therefore reminiscent of RGD sequences present in integrin-binding molecules because of its exposed loca-tion On the basis of these structural ... superimposition of the 3D structures of kistrin [32] and cardosin A (Fig 8) The importance of both motifs and in particular their basic residues was fur-ther emphasized by the complete lack of interaction...

Ngày tải lên: 30/03/2014, 11:20

... activity [26] and the pattern of channel activity is characteristic of the self-assembly of conductive oligomers Gel permeation analysis of Vpu oligomers Figure 4 Gel permeation analysis of Vpu oligomers ... restriction FRET analysis of Vpu interactions Figure 6 FRET analysis of Vpu interactions COS-1 and U2-OS cells were cotransfected with Vpu-ECFP and Vpu-EYFP expression vectors and FRET was performed ... oligomer-ization of Vpu [22] Here we have used various genetic, biochemical and biophysical approaches to characterize the oligomerization of Vpu in vitro and in vivo Molecular dynamic simulations and conductance...

Ngày tải lên: 18/06/2014, 18:20

... better understanding of the mechanisms of drug action and drug resistance may ultimately lead to new approaches for circumventing resistance and improve patient survival However, in spite of recent ... paclitaxel, and we use gene expression profiling to identify genes and pathways that may be important to the development of drug resistance in ovarian cancer Methods Cell line and generation of drug ... proteasome regulation and steroid biosynthesis for doxorubicin resistance, and control of translation and oxidative stress for paclitaxel resistance Conclusions: Ovarian cancer cells develop drug...

Ngày tải lên: 20/06/2014, 08:20

... spindle-shaped cells), macrophages (purple cells), T cells (green cells), B cells (dark blue cells) and other cells invade the subchondral bone and trigger massive local osteoclastogenesis and – as ... 5.1 per bone perimeter, covering a mean of 4% of the endosteal bone surface Figure 4 Distribution of osteoclasts and osteoblasts within local bone erosions Distribution of osteoclasts and osteoblasts ... by the detection of osteoclasts at sites of local bone erosion These cells are localized at the interphase of inflammatory tissue and bone, and are found in all animal models of destructive arthritis...

Ngày tải lên: 09/08/2014, 07:20

... IL-1b-treated cells (C,D) n-3 PUFAs inhibit cyclin D1 expression and phosphorylation of Rb Lysates of whole cells were prepared from cells treated as described above and 20 lg aliquots of protein ... control of SMC proliferation 4466 S Bousserouel et al (Eur J Biochem 271) Modulation of cyclin D1 synthesis and hyperphosphorylation of Rb by n-3 and n-6 PUFAs Induction of cyclin D1 is one of the ... for 16 h The cells were then fixed and the BrdU incorporated was quantified by using a commercial detection kit (Roche Molecular Biochemicals) Ó FEBS 2004 Anti-mitogenic action of EPA and DHA (Eur...

Ngày tải lên: 19/02/2014, 16:20

... indicated times Cells in the absence of any of these were taken as the control (Con) Two micrograms of total RNA were reverse transcribed with random primers and the levels of PEPCK and G6Pase mRNA ... Each band was analysed densitometrically and the values are depicted after normalization of PEPCK and G6Pase bands with those of 18S rRNA (B) Each point is the mean ± SEM of three sets of experiments ... primary cells and animal models but, as a preliminary step, they unravel one of the mechanisms of TNFamediated withdrawal of insulin action in HepG2 cells Results Incubation of HepG2 cells with...

Ngày tải lên: 16/03/2014, 02:20

... mM potassium glutamate and used subsequently for biochemical analysis The amount of FAD cofactor of the NifL fractions was calculated using a UV/Visual spectrum at 450 nm and the extinction coefficient ... 280 and 320 nm (MD) Preparation of inside-out vesicles of K pneumoniae One litre cultures of K pneumoniae cells were grown under nitrogen- and O2-limited conditions, harvested at a D600 value of ... absence of O2, when the flavin cofactor is reduced [16–19] Thus, under anaerobic conditions in the absence of ammonium, reduction of the flavin moiety of NifL is required to relieve NifL inhibition of...

Ngày tải lên: 17/03/2014, 10:20

... expression analysis with histomorphometry allows the exploration of any relationships between gene expression and indices of bone architecture and bone remodelling Interestingly, both OCN and ALP ... 0.008 and r = 0.70, p < 0.008, respectively; Fig- Comparison of bone structural and turnover indices between OA and control individuals Bone histomorphometry was performed on IT trabecular bone ... knowledge about the molecular and cellular mechanisms involved in the increase or maintenance of bone mass in OA This study of the IT region of the proximal femur in primary hip OA and non-OA postmortem...

Ngày tải lên: 09/08/2014, 08:23

... also found Because TLR is the product of TLRi and RA, this can be the result of the negative correlation of RA and RS and the lack of correlation between TLRi and RS However, no correlation Genome ... blue) between RA and PA, PA and TLR or TLRi, RA and TR and between TR and TLR or TLRi Some of these correlations have been described previously [8,11,17,19] The correlation between TR and TLR was ... sizes were equal to that of genes in each considered group and subsequent computation of average and standard deviations for each variable An estimation of the average standard deviations (aSE)...

Ngày tải lên: 14/08/2014, 08:20

... understanding of the influence of the molecular function is necessary, as well as of biochemical processes and of the biophysical properties provoked amongst others by a moderate repression of the ... the behaviour of the cells, which may alter the biochemical effect of the transported cargo and which could consequently influence the interpretation of the results Table List of the examined ... block and the software version 1.2 The 10µl reaction consisted of 25ng of transcribed RNA (1µl), 1pmol Universal Probe (0.1µl), 50pmol each of forward + reverse primer (0.5µl), water (3.4µl) and...

Ngày tải lên: 03/11/2012, 11:44