belo et al 1997 with modifications

... complete androgen insensitivity syndrome (CAIS) due to a complete disruption of the AR, the affected individuals are healthy 46XY individuals with a female phenotype (Lim et al. , 1997; Yong et al. , ... Deoxynucleotide triphosphates DRPLA Dentatorubral-pallidoluysian atrophy EDTA Ethylenediamine tetra-acetic acid fPSA Free PSA tPSA Total PSA FAI Free androgen index FBS Fetal bovine serum FSH Follicle-stimulating ... inversely with the age of onset of the disease (La Spada et al. , 1991) but it still uncertain if the length correlates with the severity of the disease 14 (Shimada et al. , 1995) Endocrine abnormalities...

Ngày tải lên: 11/09/2015, 09:05

... National Genotyping Center, Academia Sinica, for technical support Wu et al Journal of Biomedical Science 2010, 17:70 http://www.jbiomedsci.com/content/17/1/70 Author details School of Public Health, ... gender-adjusted ORs with the 95% CIs for the classic risk factors for the patient and control groups of the two cohorts Aging and being male gender were the two Wu et al Journal of Biomedical Science ... repeats were the two most common alleles, which is consistent with findings from previous reports [15,16] We thus selected 27 GT repeats as Wu et al Journal of Biomedical Science 2010, 17:70 http://www.jbiomedsci.com/content/17/1/70...

Ngày tải lên: 10/08/2014, 05:21

... seem associated with the ischemic stroke status only in those individuals with lowered HDL-C level from our observation and some previous studies Chen Bai et al Journal of Biomedical Science 2010, ... 0.0691) in those with lower HDL-C Bai et al Journal of Biomedical Science 2010, 17:12 http://www.jbiomedsci.com/content/17/1/12 level while those carriers with homozygous S allele (Ϲ26 GT repeats) ... study Author Details 1Central Laboratory, Shin Kong WHS Memorial Hospital, Taipei, Taiwan, 2School of Public Health, Taipei Medical University, Taipei, Taiwan, 3Changhua Christian Hospital Yunlin...

Ngày tải lên: 10/08/2014, 05:21

... instability of the femoral head with slight capsular laxity, through moderate lateral displacement of the femoral head, without loss of contact of the head with the acetabulum, up to complete dislocation ... c2 test Odds ratio (OR), P-value and 95% confidence interval (CI) were calculated with respect to the minor allele compared with the major allele Stratification analyses by gender of DDH were ... the risk allele; otherwise the common allele, D13, seemed to be a protective allele Association was detected in both female and male subjects after stratification by gender A detailed analysis...

Ngày tải lên: 12/08/2014, 15:22

... Analysis of other clinical parameters associated with disease severity (extra-articular disease/nodules, rheumatoid factor, surgery, mechanical joint score, etc.) revealed no differences between ... Faure S, Alibert O, Lasbleiz S, De Toma C, et al. : Association between tumor necrosis factor receptor II and familial, but not sporadic, rheumatoid arthritis: Evidence for genetic heterogeneity ... polymorphism with circulating sTNF-RII levels is consistent with a previous study [36] that demonstrated higher levels of sTNF-RII in healthy individuals carrying a T allele However, the association with...

Ngày tải lên: 09/08/2014, 07:20

... preparation, analysis and interpretation of data, and statistical analysis MC participated in collection of data, interpretation of data and genetic analysis MM performed genetic analysis FC was ... functional role by evaluating platelet aggregation in response to the costimulation with ADP and 5-HT [14] in healthy subjects with either one of the variants of the 5-HTR2A gene Materials and methods ... relative 95% confidence intervals were also calculated from × contingency tables Statistical significance was also evaluated using a 1,000-fold permutation test Genetic-association studies might...

Ngày tải lên: 09/08/2014, 13:21

... accordance with the guidelines detailed by the Canadian Council on Animal Care and were approved by the Carleton Animal Care Committee at Dalhousie University In situ hybridization analysis In ... mRNA, we decided to determine whether the 5¢-transcription initiation sites could be observed without using a PCR-based detection method RNase Ó FEBS 2004 4914 E A McCaw et al (Eur J Biochem 271) ... product in RPA analyses There was less primary transcript detected in the R6/2 HD striatal RNA samples compared to wild-type striatal RNA samples as determined by densitometric analysis, and the...

Ngày tải lên: 16/03/2014, 18:20

... pGalAD-AR1)14, pGalAD-AR16)36, pGalAD-AR17)32, pGalAD-AR24)39, pGalAD-AR17)32 (18/19AA), pGalADAR17)32(20/21AA), pGalAD-AR17)32(23 A), pGalADAR17)32(24/25AA), pGalAD-AR17)32(26/27AA), pGalADAR17)32(28/29AA) ... interaction system, deletion of AR3)36 (GalAD-AR.NTDD3–36) completely abolished the ligand-dependent functional N/C interaction (Fig 2A) Deletion of the N-terminal 13 amino acids (GalAD- AR.NTDD1-13) ... indicated GalADARpeptides with GalDBD-AR.LBD in yeast in the presence of lM DHT In each experiment the activity of GalAD-AR2-36 was set at 100% (see also legend to Fig 2A) (C) Western analysis of...

Ngày tải lên: 17/03/2014, 10:20

... on labial salivary gland (n = 145; n [%]) 130 (90) SD, standard deviation iation observed in only one healthy individual; and -871 T/C, which was observed among patients with pSS with a C allele ... significant differences in allele and genotype frequencies of BAFF polymorphism were detected between patients with pSS and control individuals (T allele frequency: 49.7% in patients with pSS versus 50% ... rheumatoid factor levels was analyzed using analysis of variance Statistical analysis was performed using Analyse-it for Microsoft Excel (Leeds, England, UK) Results The allelic and genotypic distribution...

Ngày tải lên: 09/08/2014, 07:20

... EE, Trikalinos TA, Countopoulos-Ioannidis DG: Replication validity of genetic association studies Nat Genet 2001, 29:306-309 Power Calculator [http://calculators.stat.ucla.edu/powercalc/] Page ... common platforms for phenotype definition, inclusion criteria, genotyping and analytical methods, and to unite the ethnically diverse resources available for study Such efforts would increase the ... Antonio Gonzalez The letter from Ikegawa and colleagues highlights some difficulties in defining what constitutes replication of previous genetic association in the context of studies with different...

Ngày tải lên: 09/08/2014, 08:22

... [http://www.ncbi.nlm.nih.gov/SNP] 34 Barrett JC, Fry B, Maller J, Daly MJ: Haploview: analysis and visualization of LD and haplotype maps Bioinformatics 2005, 21:263-265 35 Simple Interactive Statistical Analysis [http://www.quantita ... regression analysis was conducted for a subset of the material, in which all information regarding genetic factors, antibodies and matching variables was available This same sample set was used ... There are strong medical and biological arguments in favour of subgrouping RA patients, and an analysis of nonstratified samples might conceal a true association In the initial analysis of the genotype...

Ngày tải lên: 09/08/2014, 10:21

... pattern revealed a characteristic staining pattern with the terminal web and the lateral junctions of intestinal epithelial cells (Figure 1A) Discussion Based on gene expression data, chromosomal location ... A Superficial epithelium of the terminal ileum with cytoplasmic staining of the terminal web and lateral junctions of the epithelial cells (green arrow) B Staining of the superficial epithelium ... Health Sciences Center Institutional Review Board (IRB) approval and informed consent and assent Patient and control DNA were also obtained from archival colonic tissue blocks after IRB approval...

Ngày tải lên: 11/08/2014, 10:23

... to the 5,10-methylene tetrahydrofolate reductase (MTHFR) 1298 genotypes Individual and mean values (horizontal lines) expressed as the FMD percentage are shown Palomino-Morales et al Arthritis ... the values observed in individuals carrying the C allele The 677TT genotype is associated with significantly higher total plasma homocysteine levels than in heterozygotes or in individuals with ... Hospital Clínico were taking folic acid 10 mg/week Informed consent was obtained from all patients The local institutional committees approved the study To determine the potential association between...

Ngày tải lên: 12/08/2014, 12:20

... of an IL-4R polymorphism with RA severity Materials and methods Study populations and clinical evaluation Twenty patients with established RA and 26 healthy individuals were enrolled in the study ... of the Th17 cells in both healthy individuals and patients with RA are of dual Th17/Th1 lineage IL-4 generally reduced the number of IL-17+/IFNg+ cells in parallel with reductions in the number ... PBS with 10% fetal calf serum per well for hours The plates were then washed three times with 200 μl of × PBS/0.05% Page of Tween per well The standard curve was created in duplicate starting with...

Ngày tải lên: 12/08/2014, 15:22

... antimicrobial properties, antagonism of which may account for the excess mortality observed with NOS inhibition in patients with sepsis [9] Asymmetrical dimethyl arginine (ADMA) is a naturally occurring ... of asymmetrical dimethyl arginine (ADMA) and inflammatory markers Variation in asymmetrical dimethyl arginine (ADMA) levels with carriage of specific alleles at DDAH II -449 Parameter ADMA day ... Albsmeier J, Cooke JP, Boger RH: Determination of asymmetric dimethylarginine (ADMA) using a novel ELISA assay Clin Chem Lab Med 2004, 42:1377-1383 Vallance P: Importance of asymmetrical dimethylarginine...

Ngày tải lên: 13/08/2014, 03:20

... Wu et al Table VII Association analysis of the CA3 (TC)n polymorphism in 326 pigs Least squares mean values with different letters are significantly different Small letters: P < 0.05, capital letters: ... VI Association analysis of the CA3 SJ158 polymorphism in 320 pigs Least squares mean values with different letters are significantly different Small letters: P < 0.05, capital letters: P < 0.01 ... breeds with repeat numbers 17, 13 and 12, for alleles A, B and C, respectively Allele B is also prevalent in the two pig breed types, with the exception of Meishan pigs, in which allele A is prevalent...

Ngày tải lên: 14/08/2014, 13:22

... promoter (Louie et al. , 2003; Metzger et al. , 2005; Shang et al. , 2002; Wang et al. , 2005; Wang et al. , 2002; Yamane et al. , 2006), resulting in the formation of an AR transcriptional activator ... metastasis and angiogenesis in cancers (Bryant et al. , 2007; Chang et al. , 2011; Li et al. , 2010; Lu et al. , 2010; Min et al. , 2010; Richter et al. , 2009; Simon and Lange, 2008) Accordingly, ... (Systematic Evolution of Ligands by Exponential Enrichment) approach was utilized (Nelson et al. , 1999; Roche et al. , 1992; Zhou et al. , 1997) to meet this challenge Not only these studies confirm...

Ngày tải lên: 09/09/2015, 10:14

... B Zheng et al monomer availability Transcriptional regulation, mediated by cytoskeletal actin dynamics, can be attributed to modulation of the subcellular localization of transcriptional regulators ... which are also expressed in cardiac muscle and which form heterodimers with myocardin In a yeast two-hybrid screen of a skeletal muscle cDNA library using STARS as bait, Barrientos et al [58] identified ... cytoskeletal signaling cascades that converge on the AR Supervillin (SV) was initially identified from blood cells as an ABP and was found to be expressed in skeletal muscles and several cancer...

Ngày tải lên: 07/03/2014, 01:20

... (1991) Overview of metallothionein Methods Enzymol 205, 613–626 17 Vallee, B.L (1979) Metallothionein: historical review and perspectives Experientia 34(Suppl), 19–39 18 Dvir, A., Peterson, S.R., ... Chen, H.Y., Brinster, R.L & Palmiter, R.D (1984) A 12-base-pair DNA motif that is repeated several times in metallothionein gene promoters confers metal regulation to a heterologous gene Proc Natl ... beads by washing with a buffer containing 0.5 M KCl (see Materials and methods for details) Two major polypeptides of 70 and 82 kDa were detected by SDS/ PAGE (Fig 3B, lane 3) Also, there was...

Ngày tải lên: 17/03/2014, 23:20