bcl 2 regulated mitochondrial intrinsic apoptotic pathway

... human prostate cancer Mol Cancer Ther 20 05, 4:197 -20 5 Page of 10 (page number not for citation purposes) Radiation Oncology 20 09, 4:47 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 Li ZM, ... the intrinsic apoptotic pathways [36] Bcl- 2 proteins contain regions of amino acid sequence similarity, known as Bcl2 homology (BH) domains The family consists of the anti -apoptotic Bcl- 2 group ... cell lines Cancer Res 20 00, 60:6101-6110 Kang MH, Reynolds CP: Bcl- 2 inhibitors: targeting mitochondrial apoptotic pathways in cancer therapy Clin Cancer Res 20 09, 15:1 126 -11 32 Kitada S, Leone M,...

Ngày tải lên: 09/08/2014, 10:20

... cDNA: 5¢- and 3¢-primers for PAC1 (GenBank accession no Z2 327 9 for basic, Z2 327 3 for hip, Z2 327 4 for hop1, Z2 327 5 for hop2, and Z2 327 2 for hiphop1) were 5¢-TTTCA TCGGC ATCAT CATCA TCATC CTT-3¢ ... Endocrinology 1 12, 1469–1473 FEBS Journal 27 5 (20 08) 55 42 5551 ª 20 08 The Authors Journal compilation ª 20 08 FEBS S Onoue et al 26 Andersson M, Sillard R & Rokaeus A (19 92) Demonstration of [ 125 I]VIP ... PACAP27 VIP Secretin Glucagon Helodermin GLP-1 GLP-1(7–37) Control (5 · 10)6 M) Coenzyme Q10 FEBS Journal 27 5 (20 08) 55 42 5551 ª 20 08 The Authors Journal compilation ª 20 08 FEBS M) 33.7 22 .1 7.3...

Ngày tải lên: 16/03/2014, 04:20

... transformed phenotype 1 .2 Bcl- 2 and Bcl- 2 family proteins: Since the discovery of Bcl- 2, many other Bcl- 2- like proteins were subsequently discovered and documented These Bcl- 2 family proteins were ... novel function of Bcl- 2 was reported Interaction of Bcl- 2 with Nur77 led to a conformational change in Bcl- 2, exposing its BH3 domain, converting Bcl- 2 from anti -apoptotic to pro -apoptotic (Lin, ... antimycin-treated CEM /Bcl- 2 cells due to increased activity in the context of Bcl- 2 overexpression, then the observation of untreated CEM /Bcl- 2 cells consistently displaying higher mitochondrial O2- production...

Ngày tải lên: 14/09/2015, 08:42

... TZDs in 3T3-L1 adipocyte 19 20 21 22 23 24 25 26 27 28 29 30 31 32 agonist improve insulin resistance J Biol Chem 27 6, 4 124 5–4 125 4 Berger J & Moller DE (20 02) The mechanisms of action of PPARs ... (20 05) The activation of Akt ⁄ PKB signaling pathway and cell survival J Cell Mol Med 9, 59–71 Reed JC (1998) Bcl- 2 family proteins Oncogene 17, 322 5– 323 6 FEBS Journal 27 7 (20 10) 687–696 ª 20 09 ... pioglitazone and PGJ2 all induce apoptosis (Fig 2) However, TZDs not induce adipocyte apoptosis by directly binding to Bcl- 2; rather, they so by reducing Bcl- 2 levels Bcl- 2 overexpression blocks...

Ngày tải lên: 16/02/2014, 09:20

... GFP -Bcl- 2 WT Endo -Bcl- 2 100 Steady-state S/ER Cumulative 75 B % TG-induced Change in Fluo-4 Fluorescence % Cells with Numerous GFP-LC3 Punctae B * 50 25 Vector Bcl- 2- wt Bcl- 2- ER Fig Bcl- 2 control ... Bcl- 2- ER versus vector (cumulative) Furthermore, in cells expressing Bcl- 2- ER, TG reduced autophagic activity to an even greater extent 40 * 30 20 10 -10 -20 Vector Bcl- 2- wt Bcl- 2- ER ** Fig Bcl- 2 ... activation of the autophagic pathway [34] Beclin contains a Bcl- 2- binding domain which has been shown to interact with antiapoptotic Bcl- 2 and Bcl- XL, but not proapoptotic Bcl- 2 family members [35]...

Ngày tải lên: 07/03/2014, 09:20

... Greenberg AH (20 00) BNIP3 heterodimerizes with Bcl- 2 ⁄ Bcl- X(L) and induces cell death independent of a Bcl- 2 homology (BH3) domain at both mitochondrial and nonmitochondrial sites J Biol Chem 27 5, 1439–1448 ... (1999) Bcl- 2 ⁄ E1B 19 kDa-interacting protein 3-like protein (Bnip3L) interacts with bcl- 2 ⁄ Bcl- xL and induces apoptosis by altering mitochondrial membrane permeability Oncogene 18, 4 523 –4 529 Yang ... transmembrane FEBS Journal 27 8 (20 11) 134–1 42 ª 20 10 The Authors Journal compilation ª 20 10 FEBS Z Zhang et al BNIP3 in excitotoxicity B A Cell death (%) 80 Glu100 μM Glu10 μM 60 40 20 12 24 Hours 36 48...

Ngày tải lên: 22/03/2014, 17:20

... A1 BCL- 2- associated X protein BCL- 2- antagonist ⁄ killer-1 BCL2 -related ovarian killer BCL- 2 antagonist of cell death BH3-interacting domain death agonist BCL- 2- like-11 BCL- 2- modifying factor BCL- 2- interacting ... the BH3 receptor domain of Bcl- 2 or Bcl- xl, to disrupt the interaction between Bcl- 2 or Bcl- xl and Beclin In addition, Bcl- 2 ⁄ Bcl- xL phosphorylation results in Bcl- 2 ⁄ Bcl- xL dissociation from ... Journal compilation ª 20 10 FEBS F Zhou et al 24 25 26 27 28 29 30 31 32 33 34 35 36 BAX and BAK: a requisite gateway to mitochondrial dysfunction and death Science 29 2, 727 –730 Antonsson B, Montessuit...

Ngày tải lên: 28/03/2014, 23:20

... Elevated PSA 13 32 41% 100% 12 30 39% 97% 25 62 40% 98% Lung 13% 13% 13% Liver 9% 10% 10% Bone 28 88% 29 94% 57 90% Bone Marrow 0% 3% 2% Pleura 3% 0% 2% Other Prior Treatment 11 34% 29 % 20 32% Orchiectomy ... Medicine 20 10 8 :20 Received: November 20 09 Accepted: 24 February 20 10 Published: 24 February 20 10 References Jemal A, Siegel R, Ward E, Hao Y, Xu J, Murray T, Thun MJ: Cancer statistics, 20 08 CA ... 19 92, 52: 6940-6944 Banerjee PP, Banerjee S, Brown TR: Bcl- 2 protein expression correlates with cell survival and androgen independence in rat prostatic lobes Endocrinology 20 02, 143:1 825 -1832...

Ngày tải lên: 18/06/2014, 16:20

... Curcumin( M) Bcl- 2 GAPDH C Curcumin( M) 40 60 93 .25 6.47 97.31 80 37.65 19.14 23 .86 62. 33 80. 82 92. 88 72. 29 49.91 38 .27 KG1a Red 76.13 Kasumi-1 27 .68 50.05 84.76 20 .25 7 .25 0.36 15 .22 79.73 92. 74 99.63 ... Curcumin( M) 40 60 0. 02 1.81 80 2. 66 0.56 3.83 38 .27 76 .2 19.80 70.89 25 .78 57.34 0.03 7.93 0.03 17 .22 0.00 23 .89 70.95 21 .09 51.47 31 .28 41.55 34.56 5.30 0.90 19.96 0.43 12. 83 54.06 25 .08 9.95 76.79 ... Medicine 20 11, 9:71 http://www.translational-medicine.com/content/9/1/71 B Si-control Si -Bcl- 2 Si-control KG1a Bcl- 2 KG1a Si -Bcl- 2 0.05 GAPDH 1.01 0 .26 6.86 97.09 1.85 72. 80 20 .08 0.06 0.60 1.15 24 .29 ...

Ngày tải lên: 18/06/2014, 19:20

... 1998, 4 :23 2 -23 4 Jansen B, Wacheck V, Heere-Ress E, Schlagbauer-Wadl H, Hoeller C, Lucas T, Hoermann M, Hollenstein U, Wolff K, Pehamberger H: Chemosensitisation 22 23 24 25 26 27 28 29 of malignant ... Res 20 06, 16 :23 -27 54 Reed JC: Bcl- 2- family proteins and hematologic malignancies: history and future prospects Blood 20 08, 111:3 322 -3330 55 Lopes de Menezes DE, Mayer LD: Pharmacokinetics of Bcl- 2 ... Cancer Res 20 05, 24 :24 5 -25 4 39 Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2( -Delta Delta CT) Method Methods 20 01, 25 :4 02- 408 40...

Ngày tải lên: 18/06/2014, 22:20

... Research 20 09, 2: 16 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 progression of ovarian cancer: influence and source of tumor stromal cells Mol Cell Endocrinol 20 01, 175(1 -2) :29 -39 ... (11) 50 (2) 92 ( 12) 82 (14) 75 (3) 62 (8) 65 (11) 25 (1) 38 (5) 35 (6) WD MD PD Total Cancerous 19 28 100 (3) 100 (6) 68 (13) 79 (22 ) 33 (1) 67 (4) 79 (15) 71 (20 ) 67 (2) 33 (2) 21 (4) 29 (8) 100 ... (2) 100 (5) 50 (2) 82 (9) 0 50 (2) 18 (2) 100 (2) 100 (2) 100 (2) 100 (2) Cysts Cystadenomas Total Benign 13 17 100 (4) 100 (13) 100 (17) 25 (1) 38 (5) 35 (6) 75 (3) 62 (8) 65 (11) 50 (2) ...

Ngày tải lên: 20/06/2014, 07:20

... enhancer chuỗi nặng gen kháng thể sản xuất B tế bào ung thư kết quảcủa lymphoma Burkitt Các chuyển vị BCL- locus nhiều đột biến làm phát sinh ác tính tế bào B Tất bệnh bạch cầu dẫn bệnh bạch cầu mãn...

Ngày tải lên: 01/08/2014, 21:20

... (G3, day 12 PI), lanes and 8; EAE (R0, day 21 PI) Immunohistochemical Localization of Bcl- 2 in the Spinal Cords of Rats with Experimental Autoimmune Encephalomyelitis 28 1 Table Bcl- 2 immunoreactivity ... cl -2 in EAE Bcl- 2 was constitutively expressed in the normal rat spinal cord (Fig 1, lane 1), and expression increased in response to immunization with CFA (Fig 1, lane 2) The degree of Bcl- 2 ... suggests that the decreased expression of Bcl- 2 in EAE by western blot might come from the less immunoreactivity of Bcl- 2 in host cells, or the amount of Bcl- 2 expression in normal spinal cords overwhelm...

Ngày tải lên: 07/08/2014, 15:20

... 20 05, 3 12: 1 12- 119 24 25 26 27 28 29 Xiao Y, He J, Gilbert RD, Zhang L Cocaine induces apoptosis in fetal myocardial cells through a mitochondria-dependent pathway J Pharmacol Exp Ther 20 00, 29 2:8-14 ... damage Proc Natl Acad Sci U S A 1997, 94 :20 07 -20 12 46 Adams JM, Cory S The Bcl- 2 protein family: arbiters of cell survival Science 1998, 28 1:1 322 -1 326 3 02 ... grants HL- 827 79, HL-83966 (L.Z.) and by the California Tobacco-Related Disease Research Program Award 14FT-0075 (D.X.) Conflict of Interest 301 10 11 12 13 14 15 16 17 18 19 20 21 22 23 The authors...

Ngày tải lên: 08/08/2014, 17:20

... Apoptosis is regulated by members of the Bcl- 2 family A1, one of the antiapoptotic Bcl- 2 family members, is described as being important for the survival of mast cells during allergic reactions .20 To ... expression of forkhead and Bcl- 2 family members Scand J Immunol 20 06;63:1–6 28 Shinjyo T, Kuribara R, Inukai T, et al Downregulation of Bim, a proapoptotic relative of Bcl2 , is a pivotal step in ... TNP7MAR F(ab´ )2, functions as a multivalent antigen recognized by the Fc⑀RI-bound IgE but also recognizing bound 2. 4G2 rat Ab.13 Aggregation with 2. 4G2 rat Ab together with TNP7-MAR F(ab´ )2 does not...

Ngày tải lên: 08/08/2014, 21:20

... cells Gynecol Oncol 20 07, 105(1) :21 8 -22 2 Kang MH, Reynolds CP: Bcl- 2 inhibitors: targeting mitochondrial apoptotic pathways in cancer therapy Clin Cancer Res 20 09, 15(4):1 126 -11 32 Lin B, Kolluri ... NF-κB activities [14-17 ,28 ] Interestingly, the apoptotic effect of arsenic trioxide largely depends on a Bcl- 2- controlled pathway [ 12, 18 -20 ] Bcl- 2, an anti -apoptotic Bcl- 2 family member, for which ... Gastroenterol 20 02, 8(1):36-39 16 Bowling BD, Doudican N, Manga P, Orlow SJ: Inhibition of mitochondrial protein translation sensitizes melanoma cells to arsenic trioxide 24 25 26 27 28 29 30 31 32 33...

Ngày tải lên: 09/08/2014, 03:21

... Surgical Oncology 20 08, 6:65 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 A New American Joint Committee on Cancer staging system for cutaneous melanoma Cancer 20 00, 88:1484-1491 ... expression of bcl- 2 in three types of metastases bcl- 2 positive (%) bcl- 2 negative (%) Total (%) 26 (74.3) 24 (85.7) 14 ( 82. 3) 64 (80.0) (25 .7) (14.3) (17.7) 16 (20 .0) 35 (100) 28 (100) 17 (100) ... 4:1865-1871 Leiter U, Schmid RL, Kaskel P, Peter RA, Krähn G: Antiapoptotic bcl- 2 and bclxL in advanced melanoma Arch Dermatol Res 20 00, 29 2 :22 5 -23 2 Balch CB, Buzaid AC, Atking MB, Cascinelli N, Coit DG,...

Ngày tải lên: 09/08/2014, 07:21

... Bcl- 2 (Bcl- 2 WT), cytosolic Bcl- 2 lacking the transmembrane domain (Bcl- 2 ΔTM) and Bcl- 2 mutants restricted to the outer mitochondrial membrane (Bcl- 2 ActA) or to the endoplasmic reticulum (Bcl- 2 ... phospholipase A2 Nat Immunol 20 03, 4:387-393 Page of (page number not for citation purposes) Radiation Oncology 20 09, 4:41 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 He S, Wang L, ... metabolism ? Bcl- 2 Bcl- 2 Bcl- 2 Bcl- 2 ER Crosstalk Bcl- 2 Bcl- 2 Mito ? Nucleus ? ROS, PARP, NAD+/ATP-depletion, AIF, ROS, PARP, NAD+/ATP-depletion, AIF, Cathepsins, Calpains, EndoG, HtrA2/OMI Cathepsins,...

Ngày tải lên: 09/08/2014, 10:20