bài 16 tiêu hóa tiếp theo

... at station 3, when sensed at station 4, is x 7/i2 = 7/6 = 1 .16 or a vector of 1 .16 at 180° By taking the square root of 0.752 plus 1 .162 , we get a resultant of 1.38 oz-in Now to get the proper ... in Fig 23 .16 When the structure vibrates, each point of light "stretches" into a line as in Fig 23.l6b The length of any line is estimated by comparing it with the rulings In Fig 23 .16& , the ... waveforms of instantaneous displacement versus time on a paper strip As paper speed was known, Fig 23 .16 Vibration measurement with a microscope Fig 23.17 Hand-held vibration measurement instrument...

Ngày tải lên: 25/10/2013, 13:15

... CuO(s) Fe2O3(s) H2O2(l) H2O(l) HCl(g) HI(g) I2(g) NH3(g) NO2(g) O2(g) SO2(g) -166 9.8 +30.71 -393.5 -635.5 -156.1 -822 .16 -187.8 -285.83 -92.30 +25.94 +62.25 -46.19 +33.84 -296.9 Entropy • entropy ... Don’t get ahead of the game • There is a lot of theory in this chapter • Keep terms separate (5), and equations Universe, System, Surrounds, Enthalpy, ... Energetically Equivalent States for the Expansion of a Gas Tro, Chemistry: A Molecular Approach 16 Macrostates → Microstates These microstates all have the same macrostate So there are differentThis...

Ngày tải lên: 28/11/2013, 01:12

... Angle (°) D165-M-H79 H79-M-H169 H79-M-wata Wata-M-H169 H169-M-D165 H31-M-D165 H31-M-watb a Fe 0.06 ± 0.01 0.03 ± 0.02 0.04 ± 0.03 104.8 ± 1.3 136.1 ± 0.8 97.2 151.5 77.1 74.5 111.1 82.1 169 .5 118.8 ... structure of SOD from A pernix K1 A WAT D165 (OD2) H169 (NE2) H79 (NE2) H31 (NE2) B WAT H169 (NE2) D165 (OD2) H79 (NE2) WAT H31 (NE2) C WAT D165 (OD2) H169 (NE2) H79 (NE2) H31 (NE2) D E FEBS Journal ... (Asp165 and His169) from the C-terminal domain (Fig 3) In Mn-reconstituted ApeSOD, the metal ion was five-coordinate in trigonal bipyramidal geometry (Fig 3A) Three of the ligands, OD2 of Asp165,...

Ngày tải lên: 14/02/2014, 22:20

... maturation targets the protein capsid to concerted disassembly and unfolding J Biol Chem 275, 160 37 160 43 Oliveira, A.C., Ishimaru, D., Goncalves, R.B., Smith, T.J., ¸ ´ Mason, P., Sa-Carvalho, ... bacteriophage P22 coat protein and its entropic stabilization in the icosahedral shells Biophys J 66, 163 1 164 1 23 Leimkuhler, M., Goldbeck, A., Lechner, M.D & Witz, J (2000) Conformational changes preceding ... wildtype and temperature-sensitive protein subunits of the phage P22 capsid J Biol Chem 270, 165 95 166 01 52 Teschke, C.M & King, J (1995) In vitro folding of phage P22 coat protein with amino...

Ngày tải lên: 19/02/2014, 12:20

... Corynebacterium callunae (CcStP), which has been characterized biochemically and structurally [15 ,16] , for particular reason The intersubunit contacts stabilizing the functional CcStP dimer are ... were produced as described elsewhere [15,18] Natural CcStP was puried by a reported procedure [16] If not stated otherwise, recombinant CcStP was used EcMalP was prepared according to Eis et ... of resolution Apo-EcMalP was prepared using a protocol developed by Palm and coworkers (D Palm, Theodor-Boveri-Institut fur Biowissenschaften, Universitat Wurzburg, Germany; ă ă ă personal communication)...

Ngày tải lên: 19/02/2014, 16:20

... References 156 157 159 160 160 165 165 165 167 167 167 168 168 169 171 171 171 172 173 173 177 178 178 179 179 179 179 179 181 182 183 183 ... Vitamins Chapter 16 Analytical Considerations 16. 1 Bioassays 16. 2 In Vitro Analytical Techniques 16. 3 Analytical ... 16. 5 Method Evaluation 16. 5.1 Measurement Value and Uncertainty 16. 5.2 Quality Assurance 16. 5.3...

Ngày tải lên: 08/03/2014, 00:20

... protein (residues 5–479) was generated from the rat HDC sequence (Swiss-Prot accession number P16453) using the automated comparative protein modeling server SWISS-MODEL [22–24] in First Approach ... generously supplied by L Persson (Lund University, Sweden) Recombinant Strep-tag unstained standards 161 –0362 (Bio-Rad, USA) were used as references for electrophoretic migrations In each experiment, ... Alignment of rat HDC and pig DDC sequences The 5–479 fragment of rat HDC (Swiss-Prot accession number P16453) and pigDDC sequence from the Protein Data Bank (PDB ID 1JS6) were aligned using the ProdModII...

Ngày tải lên: 08/03/2014, 08:20

... Heikemper, N.P Vela, K.R Stewart, C.S Westphal, J Anal At Spectrom 16 (2001) 299–306 [15] P Quevauviller, Trends Anal Chem 19 (2000) 86–96 [16] P Quevauviller, E.A Maier, B Griepink, S Caroli, Elem Speciation ... Extraction efficiency number of extraction Water Rice (0.182 ± 0.031) 1st 2nd Extractions n = Chicken (0 .168 ± 0.002) Methanol:water M H3 P04 1:1 9:1 1:1–9:1 77.0 ± 2.0 9.1 ± 1.0 93.0 ± 3.0 80.0 ± 4.0 ... 25.0 ± 1.7 90.0 ± 3.1 58.0 ± 2.2 27.0 ± 2.0 92.0 ± 3.0 56.0 ± 2.1 24.0 ± 1.9 85.0 ± 2.8 58.0 ± 2.8 16. 6 ± 1.1 90.0 ± 3.0 – – – Soil (631.8 ± 3.0) 1st 2nd Extractions n = 50.0 ± 3.0 28.0 ± 1.9 85.0...

Ngày tải lên: 15/03/2014, 23:49

Ngày tải lên: 22/03/2014, 11:20

... cannot grow by leveraging But, we have now turned the whole finance theory on its head This is because all economic theory and finance theory start from the assumption of normality But we are not living ... Crisis World” 15 16 November 2011, Mumbai Day – 15 November 2011 11.45–12.45 Inaugural session - Addresses by D Subbarao, Governor, RBI and Jaime Caruana, General Manager, BIS 14.00 16. 00 Session ... excerpts may be reproduced or translated provided the source is stated ISSN 160 9-0381 (print) ISBN 92-9131-088-3 (print) ISSN 168 2-7651 (online) ISBN 92-9197-088-3 (online) Preface The failure of regulation...

Ngày tải lên: 23/03/2014, 02:20

... C45T, A226G C469G, G691T,C1441T C698T,C1239T,G1597A G184A, C1317T, G1516A G99A, G495A, A1159G G1221T, G1839T A680G C123T, G 1165 C, A1509T C58T, G444T, T1793C N76D P157A, D231Y P234L, G554S E62K, ... Rational engineering based on these data resulted in the construction of a highly efficient polymerase [16] Further rational improvement of catalytic efficiency or stability would benefit from comparisons ... kcat1 ⁄ Km1 Km2 kcat2 (s)1ÆmM)1) (mM) (s)1) kcat2 ⁄ Km2 (s)1ÆmM)1) 0.74 0.60 1.19 0.19 0.14 0.28 71 165 82 1.4 1.7 2.2 0.020 0.010 0.026 0.35 0.18 0.54 0.14 0.23 0.23 29 48 62 0.52 0.43 0.86 0.018...

Ngày tải lên: 23/03/2014, 11:20

... the system will be: Cp ¼ where Eqn (12) DGU;m ¼ DHU;m À Tm ÁDSU;m ¼ ÀRÁTm ÁlnðCt Þ2 Eqn (16) By using Eqn (16) , Eqn (15) can be expressed in terms of DHU,m: DSU ¼ DHU;m þ 0:5ÁRÁln ðCt Þ þ Tm Z T ... (°C) ± ± ± ± ± ± 0.2 0.2 0.2 0.2 0.3 0.3 DGU,25 (kJÆmol)1) Tg (°C) 183 175 174 166 106 100 28.5 26.7 26.6 25.0 16. 0 15.4 101.9 98.0 97.8 94.2 74.4 73.0 ± ± ± ± ± ± 2 2 3 ± ± ± ± ± ± 0.3 0.3 0.3 ... associated with mean zero enthalpy change J Mol Biol 116, 429–442 29 Privalov, P.L (1979) Stability of proteins Small globular proteins Adv Protein Chem 33, 167 –241 30 Cobos, E.S., Filimonov, V.V., Galvez,...

Ngày tải lên: 23/03/2014, 12:20

... DS° (kJÆmol)1ÆK)1) DG° (kJÆmol)1) FN° 5.5 5.5a 6.5 6.5b 7.0 41.6 47.3 51.4 51.9 54.0 138 153 165 165 174 2.8 2.8 3.1 3.0 3.0 82 82 76 74 74 0.26 0.25 0.22 0.21 0.21 6.9 8.2 9.8 9.9 11.0 0.91 ... Materials and methods Preparation of protein and DNA samples The previously constructed pPG14 plasmid [16] , containing the HK022 Xis gene linked with a His-tag and a thrombin cleavage site at the protein ... in HK022 Xis [14] Their binding sites are very similar and the proteins can be interchanged [14 16] Structural studies of Xis were hampered by protein instability and a high intracellular toxicity...

Ngày tải lên: 23/03/2014, 15:21

... (JÆmol)1) DHvh/DHcal coiled-coil transition 486 281 258 182 120 108 75 58 )9280 )3610 )3170 )166 0 – )1 216 )630 )515 )7600 )3080 )2640 )1170 )687 )656 – – – )530 )530 )490 – )560 – )515 0.13 0.39 ... 227, 680–685 16 Schagger, H & Jagow, G (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from to 100 kDa Anal Biochem 166 , 368–379 ... fibritin mutants in 0.01 M Na phosphate buffer (pH 8.0) and 0.15 M NaCl Protein concentration was 16 mM chain)1 for the full-length fibritin, and 50 mM chain)1 for the others (a) Thermal transition...

Ngày tải lên: 24/03/2014, 03:21

... supported the idea that heme attachment takes place after the apoprotein has left the cytoplasm [16] Effect of thiol–disulfide redox conditions A defect in an integral membrane protein, DsbD (also ... targeted to the periplasm by fusing the signal peptide of bacterial cytochrome c at the N-terminus [16] Periplasmically expressed exogenous cytochromes c in host cells so far have spectrophotometric ... (2000) Haem–polypeptide interactions during ¨ cytochrome c maturation Biochim Biophys Acta 1459, 316 324 Page, M.D., Sambongi, Y & Ferguson, S.J (1998) Contrasting routes of c-type cytochrome assembly...

Ngày tải lên: 24/03/2014, 03:21

... -0.1315* 0.0142 0. 1168 * -0.2018* 0.2472* 0 .169 8* 0.4214* -0.0449* 0.2048* -0.1870* -0.2298* -0.0195 0.0286* -0.1486* 0.0879* 0.1480* 0.2102* 0.0683* -0.1956* -0.2904* -0.0 616* 0.0297 -0.1718* ... 1.991*** [0.522] -0.053*** [0.006] 0.685** [0.297] -0.004 [0.007] [0.005] 16. 569*** [1.481] x 2162 7 0.13 x 21993 0.43 x 168 36 0.25 x 19788 0.19 x 11534 0.26 33 Maturity match (9) Stability Return ... -0 .168 ** [0.068] -6.127*** [1.559] -0.004 [0.017] -5.713*** [0.864] 0.142 [0.154] 0.759*** [0 .166 ] 0.386*** [0.139] 113.066*** [4.460] Year fixed effects x x x Number of observations 12512 2161 1...

Ngày tải lên: 29/03/2014, 08:20

... 0.10 162 2 % Variation second derivative 0.06 1530 ΔAbs (a.u.) 0.04 0.02 0.00 –0.02 –0.04 1530 cm–1 1552 cm–1 162 2 cm–1 165 1 cm–1 1.0 0.08 1552 –0.06 0.8 0.6 0.4 0.2 0.0 165 1 –0.08 1700 165 0 160 0 ... 50 60 70 40 Temperature (°C) 162 2 0.12 0.10 0.08 0.06 0.04 0.02 1530 0.00 –0.02 –0.04 –0.06 –0.08 –0.10 –0.12 –0.14 1552 80 90 80 90 160 8 cm–1 162 2 cm–1 163 5 cm–1 169 3 cm–1 1.0 % Variation second ... 169 3 cm–1 1.0 % Variation second derivative ΔAbs (a.u.) C 0.14 1500 0.8 0.6 0.4 0.2 0.0 165 1 1700 165 0 160 0 Wavenumber 1550 1500 20 30 40 50 60 70 Temperature (°C) (cm–1) Fig Attenuated total...

Ngày tải lên: 30/03/2014, 02:20

... contrast, the W243I mutant was found to exhibit a remarkably higher E274nm ⁄ E455nm ratio (ranging from 16 to 25, depending on the enzyme preparation), indicating a significantly lower content of flavin ... elution volume corresponding to a monomeric form of DAAO at all protein concentrations tested (0.2– 16 mgÆmL)1) We previously reported that yeast DAAO is a stable dimer and that it converts to a monomeric ... previously [11] c These values were estimated using a three-state model, as described previously [11 ,16] sensitive to temperature than the wild-type DAAO (Table 2) Taken together, these data show that...

Ngày tải lên: 30/03/2014, 11:20

... of membrane proteins in the cell-free cysteine J Bacteriol 185, 1161 – 1166 system was not related to the number of transmembrane 16 Bullock, W.O., Fernandez, J.M & Stuart, J.M (1987) XL1-Blue: ... polypeptides in high yield Science 242, amino acid selective labeling of membrane proteins without 1162 – 1164 any losses in the protein yields Together with the fast Alakov, Y.B., Baranov, V.I., Ovodov, ... in a Balzers 400T freeze-fracture apparatus (Balzers, Lichtenstein) with the specimen stage at )160 °C Platinum/carbon shadowing was at 45° (with respect to the specimen stage) whereas pure carbon...

Ngày tải lên: 30/03/2014, 13:20