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Báo cáo khoa học: Optimization of an Escherichia coli system for cell-free synthesis of selectively 15N-labelled proteins for rapid analysis by NMR spectroscopy pdf

Báo cáo khoa học: Optimization of an Escherichia coli system for cell-free synthesis of selectively 15N-labelled proteins for rapid analysis by NMR spectroscopy pdf

... for Saccharomyces carlsbergensis, no data available for E. coli; b value for Lupinus luteus and bovine, no data available for E. coli; c value for Paracoccus denitrificans, no data available for ... reaction mixture with ammonium or potassium acetate. In addition, the activity of peptide deformylase a ppeared to be generally reduced in the cell -free expression system. Keywords: cell -free protein ... acids chosen for hCypA labelling were 50 l M for [ 15 N]Trp and [ 15 N]Tyr, 150 l M for [ 15 N]Ile, [ 15 N]Thr and [ 15 N]His, 0.35 m M for remaining Group II [ 15 N]amino acids, and 1m M for those...

Ngày tải lên: 16/03/2014, 18:20

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The Fight for a Free Sea: A Chronicle of the War of 1812 pot

The Fight for a Free Sea: A Chronicle of the War of 1812 pot

... Detroit River the day before, formed within musket shot of the American lines and opened the attack with a battery of three-pounders. They might have rushed the camp with bayonet and tomahawk ... carronades. He was not afraid to risk a duel with the Phoebe even with this handicap in armament, but the sloop-of-war Cherub was a formidable vessel for her size and the Essex, Junior, which was ... reputation for courage and conduct, he intended to leave nothing to the chances of fortune which might decide a combat between equals. He therefore would accept no provocation to fight without the...

Ngày tải lên: 17/03/2014, 15:20

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