and anti apoptotic bcl 2 family proteins

... targeted therapies ABT-263 and its structurally related compound ABT-737 are potent inhibitors of the anti-apoptotic proteins Bcl-2, Bcl-xL, and Bcl-w, but not of Mcl-1, and induce apoptosis in ... et al BMC Cancer (2016) 16:531 DOI 10.1186/s12885-016-2600-y RESEARCH ARTICLE Open Access Pro-survival responses to the dual inhibition of anti-apoptotic Bcl-2 family proteins and mTOR-mediated ... Bratislava, Slovak Republic), and against Mcl-1, Risberg et al BMC Cancer (2016) 16:531 Bcl-2, Bcl-xL, caspase-3, mitogen-activated protein kinase3/1 (ERK1/2), p-ERK1/2(Thr202/Tyr204), AKT, pAKT(Ser473),...

Ngày tải lên: 20/09/2020, 15:08

... known as Bcl-2 homology (BH) domains The family consists of the anti-apoptotic Bcl-2 group (such as Bcl-2, Bcl-XL, Mcl-1), the pro-apoptotic Bax group (Bax, Bak and Bok) and the pro-apoptotic ... of anti-apoptotic Bcl-2 family proteins It induces apoptosis in a wide range of tumor cell lines and enhances chemotherapy- and radiation-induced cytotoxicity both in vitro and in vivo Bcl-2 and ... it phos-phorylates and inactivates anti-apoptotic Bcl-2 family members, including Bcl-2, Bcl-XL and Mcl-1 [49-51] Finally, other investigators have recently shown that Bcl-2 antagonists like...

Ngày tải lên: 09/08/2014, 10:20

... apoptosis is regulated by Bcl-2 family proteins which regulate cell survival and is comprised of Bcl-2, Bcl-XL, Bcl-W, Bcl-2-A1, and Mcl-1 Studies show that deregulation of the Bcl-2 as primary oncogenic ... analysis of the anti-apoptotic protein Bcl-2 and Mcl-1 have shown that the anti-apoptotic genes such as Bcl-2 and Bcl-xL were overexpressed in CSC while the ex-pression of pro-apoptotic genes ... either Bcl-2 or Mcl-1, providing a survival dependency that pre-dicts the activity of Bcl-2 antagonists [18] Analogous to its predecessor ABT-737, ABT-263 possesses high affinity for Bcl-xL, Bcl-2,...

Ngày tải lên: 17/06/2020, 18:33

... no Z23279 for basic, Z23273 for hip, Z23274 for hop1, Z23275 for hop2, and Z23272 for hiphop1) were 5¢-TTTCA TCGGC ATCAT CATCA TCATC CTT-3¢ (sense) and 5¢-CCTTC CAGCT CCTCC ATTTC CTCTT-3¢ (antisense); ... the control and STZ-treated groups with respect to the expression of Bcl-2 and Bcl-XL, showing a 63% and 74% decrease of Bcl-2 and Bcl-XL, respectively (Fig 6B,C) Although both PACAP27 and PACAP38 ... pro-apoptotic and anti-apop-totic members that interact at both the physical and functional level to regulate apoptotic cell death [35] The pro-survival members (Bcl-2 and Bcl-XL) oppose two pro-apoptotic...

Ngày tải lên: 16/03/2014, 04:20

... expression.22 Antibodies and Reagents AffiniPure rabbit anti-mouse IgG (RAM IgG), AffiniPure RAM IgG F(ab´)2 fragment (RAM F(ab´)2), and AffiniPure mouse anti-rat IgG (H+L) F(ab´)2fragment (MAR F(ab´)2) ... regulates phosphorylation and expression of forkhead and Bcl-2 family members Scand J Immunol 2006;63:1–6 18 Alfredsson J, Puthalakath H, Martin H, et al Proapoptotic Bcl-2 family member Bim is involved ... with 2.4G2 rat Ab, and (as a consequence) coaggregate FcRI and FcRIIB in cells sensitized with both IgE and 2.4G2 rat Ab Since aggregation using 2.4G2 rat Ab together with TNP7-MAR F(ab´)2 does...

Ngày tải lên: 08/08/2014, 21:20

... family of proteins (Youle and Strasser, 2008) All pro-apoptotic and pro-survival (anti-apoptotic) proteins belong to the Bcl-2 family (Reed et al., 1996) Bcl-2 protein counteracts Bax, and when ... (Roth and Hansen, 2004) Apoptosis is regulated by the interplay of the pro- and anti-apoptotic (pro-survival) factors, involving chiefly members of the B-cell lymphoma/ leukemia 2 (BCL-2, Bcl-2) family ... related to apoptosis (pro-apoptotic Bad and Bax; and anti-apoptotic Bcl-2) during oocyte maturation and the ameliorating effects of select antioxidants- viz melatonin and zinc In the experiment,...

Ngày tải lên: 14/01/2020, 14:22

... families and domains [18] A46, A52, B15 and K7 are included in a single family (Pox_A46) together with other poxvirus proteins like VACV C6 and C16/B22, whereas N1 is classified in the Orthopox_N1 family ... (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and Trang 2to BH3 peptides from pro-apoptotic proteins Bid, Bimand Bak [12] and even inhibits the increase in ... alpha-helix 2 is observed in N1, K7, B15 and A52 structures and it can be predicted in other proteins from their sequences In N1 protein residues projecting outwards from alpha-helix 2 include Asp22,...

Ngày tải lên: 12/08/2014, 04:20

... May 2009, accepted 29 May 2009) doi:10.1111/j.1742-4658.2009.07123.x Protein disulfide isomerase (PDI) and other PDI family proteins are mem- bers of the thioredoxin superfamily and are thought to ... The cDNAs encode proteins of 520 and 523 amino acids, and have been denoted GmPDIL-3a and GmP- DIL-3b, respectively. GmPDIL-3a and GmPDIL-3b are the first plant ER PDI family proteins reported ... spectrophoto- metrically at 320 nm (25 °C) in the absence or presence of 1.2 lm recombinant GmPDIL-3a, GmPDIL-3b, and GmPDIL-2. Antibody production Guinea pig antiserum specific for GmPDIL-3a and rabbit antiserum...

Ngày tải lên: 18/02/2014, 11:20

... Cumulative Bcl-2-wt Bcl-2-ER * 0 25 50 75 100 Vector Bcl-2-wt Endo-Bcl-2 GFP-Bcl-2 Bcl-2-ER Fig 5 Bcl-2 control of autophagic activity HL-1 cells were trans-fected with mCherry-LC3 and empty ... autophagic pathway [34] Beclin 1 contains a Bcl-2-binding domain which has been shown to interact with antiapoptotic Bcl-2 and Bcl-XL, but not proapoptotic Bcl-2 family members [35] How-ever, the nature ... CA 92182-4650, USA Fax: +1 619 594 8984 Tel: +1 619 594 8981 E-mail: robbieg@sciences.sdsu.edu (Received 17 July 2006, revised 23 April 2007, accepted 27 April 2007) doi:10.1111/j.1742-4658.2007.05849.x...

Ngày tải lên: 07/03/2014, 09:20

... Samali A (2009) Bcl-2 family on guard at the ER Am J Physiol Cell Physiol 296, C941–C953 22 Reed JC (2006) Proapoptotic multidomain Bcl-2 ⁄ Baxfamily proteins: mechanisms, physiological roles, and ... Bcl-2 and Bcl-xL in oncogenesis and tumor progression It may be useful for future improvement of cancer treatment by modulating the two processes Bcl-2 and Bcl-xL in the apoptosis Bcl-2 and Bcl-xL ... [23–25] Bcl-xL and Mcl-1, but not Bcl-2, have been shown to target Bak, whereas all of the antiapoptotic members interact with Bax to inhibit apoptosis [26–29] The antiapoptotic function of Bcl-2...

Ngày tải lên: 28/03/2014, 23:20

... Cancer Res 2005, 24:245-254. 39 Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta CT) Method Methods 2001, 25:402-408. 40 ... local control and survival in a spontaneous canine model of primary mucosal melanoma Melanoma Res 2006, 16:23-27. 54 Reed JC: Bcl-2-family proteins and hematologic malignancies: history and future ... Real-time PCR of bcl-2 mRNA expression GAPDH mRNA expression was used as internal control Panel D: Relative level of bcl-2 gene expression calculated as a ratio of the quantity of bcl-2 and GAPDH PCR...

Ngày tải lên: 18/06/2014, 22:20

... Aa, Ab and A16 for OEA-A; Bb, Be, Bd, Be, Bf, Bg, Bh, Bi, BF3, BF35, BF38 and BF418 for OEA-B; Ca, Cb and CF5 for OEA-C; Da for OEA-D; Ma and Mb for OEA-M; R and 0 for OEA-R; F30 and F275 Hemolytic ... between the 2 groups Blood group antigens Among the 25 sheep reagents tested, 23 yielded positive results in the mouflon The A16 and Bd blood factors were not found while all the 172 mouflons ... result of mainland introductions from native populations on Corsican and Sardinian islands (Tomiczek, 1985; Uloth and Prien, 1985) Numerous European countries (from Spain to ex-USSR) and other parts...

Ngày tải lên: 09/08/2014, 18:21

... Free Dry Milk and subjected to immunoblotting using anti-bodies against individual human Bcl-2 family proteins [Apoptosis Bcl-2 Family Antibody Sampler Kit or Pro-Survival Bcl-2 Family Antibody Sampler ... the blocking of heterodimerization between anti-apoptotic members, like Bcl-2, Bcl-XL, and Mcl-1, and pro-apoptotic members like Bax and Bak of the Bcl-2 family [33] Our demonstration that TW-37 ... cell lines and increase the fre-quency of apoptotic cells in fresh patient cultures (Fig 1) Inventory of Bcl-2 family protein by Western-blot quantification of anti-, pro-apoptotic Bcl-2 family protein...

Ngày tải lên: 10/08/2014, 22:20

... NF177 10-6M PGE2 (pg/well) 6-keto-PGF1α (pg/ well) 58 ± 18 275 ± 22 288 ± 17 277 ± 28 280 ± 22 268 ± 17 256 ± 22 292 ± 16 888 ± 30 4055 ± 22 3990 ± 38 3885 ± 33 3905 ± 55 3875 ± 42 4005 ± 35 4055 ... M, Sridhar SK: Synthesis and pharmacological activities of 1, 8naphthyridine derivatives Biol Pharm Bull 2002, 25:798-802 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 Roma G, Di ... 6-keto-PGF1α (pg/ well) 45 ± 280 ± 29 295 ± 31 285 ± 23 288 ± 28 260 ± 21 277 ± 15 300 ± 24 797 ± 23 3391 ± 98 3508 ± 82 3330 ± 49 3452 ± 74 3660 ± 84 3280 ± 94 3220 ± 77 (b) Control IL-1α NF161 10-4M...

Ngày tải lên: 11/08/2014, 08:21

... transformed phenotype 1.2 Bcl-2 and Bcl-2 family proteins: Since the discovery of Bcl-2, many other Bcl-2-like proteins were subsequently discovered and documented These Bcl-2 family proteins were generally ... group of Bcl-2 family proteins is anti-apoptotic and contains BH1-4 domains These include Bcl-2, Bcl-xL, Bcl-w and Mcl-1 (Strasser 2005) The second group of members is pro-apoptotic and contains ... binding and inhibition of anti-apoptotic Bcl-2 proteins or the direct activation of Bax and Bak They also exhibit varying specificities in their binding to other Bcl-2 family members (Willis and...

Ngày tải lên: 14/09/2015, 08:42

... _ 39 4.2.2 Establishment of Neuro2A cell models stably expressing GFRα2 isoforms 42 4.2.3 GFRα2 isoforms differentially activated ERK1/2 and Akt 43 4.2.4 [ 125 I]GDNF bound ... that GDNF and NTN activate MAPK signaling by phosphorylation on Thr202/204 of ERK1/2 through GFRα2 Figure 3.2 GDNF and NTN induced MAPK (ERK1/2) phosphorylation in BE(2)-C cells A, BE(2)-Cells ... GFRα2 .17 3.1 Background and objectives _ 18 3.2 Results _ 21 3.2.1 Neuroblastoma BE(2)-C cells express GFRα2, NCAM and RET _ 21 3.2.2...

Ngày tải lên: 14/09/2015, 13:13

... between Erk and Bcl-2 Signaling Pathways Yong Wen1, 2, Yawen Ji1, 2, Yunpeng Zhang1, 2, Baoqi Jiang1, 2, Cuizhu Tang1, 2, Qi Wang1, 2, Xiyan Chen1, 2, Linglu Jia1, 2, Weiting Gu3, Xin Xu1, 2 School ... Road, Jinan, Shandong, 250012 P.R China Tel./Fax: +86-531-82169268 Xin Xu (xinxu@sdu.edu.cn) No 44-1, Wenhua Xi Road, Jinan, Shandong, 250012 P.R China Tel./Fax: +86-531-88382923 © Ivyspring ... Int J Med Sci 2017, Vol 14 Ivyspring International Publisher 1231 International Journal of Medical Sciences 2017; 14(12): 1231-1240 doi: 10.7150/ijms.20504 Research Paper Knockdown...

Ngày tải lên: 15/01/2020, 21:35

... Hematology & Oncology 20 09, 2: 50 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 Salvesen GS, Riedl SJ: Caspase Mechanisms Adv Exp Med Biol 20 08, 615:13 -23 Sulston JE, Horvitz ... BH1 and BH2 [19] Members of this family fall into three main groups based on their structure and function: the anti- apoptotic proteins, which include Bcl- 2 and Bcl- XL; the pro -apoptotic proteins, ... by the release of pro -apoptotic molecules into the cytoplasm from mitochondria These molecules belong to the Bcl- 2 family of proteins Bcl- 2 and Bcl- XL are anti- apoptotic proteins that reside in...

Ngày tải lên: 10/08/2014, 22:20

... 22 0 Chapter 12: Marketing Yourself and Your Properties Online and in Print 22 1 Targeting Your Marketing Message 22 2 Defining your target audience 22 2 ... 22 4 Creating and Placing High-Impact Ads 22 7 The power of a great headline 22 7 Writing high-impact advertising copy .22 8 Choosing the right media outlets 23 0 Converting ... Ready for Showing 20 3 Chapter 12: Marketing Yourself and Your Properties Online and in Print 22 1 Chapter 13: Negotiating the Contract and Closing the Deal .24 5 Part IV: Running a Successful...

Ngày tải lên: 27/03/2014, 01:27