an in vitro study of biomineralization

... concentration) Antimicrobial and antibiofilm activity The effect of AgNPs and AgNO3 on antimicrobial activity against 27 strains of anaerobic bacteria and 6 reference strains was investigated ... area to effectively interact with a certain microorganism In general, large surface area of nanoparticles enhances the interaction with microbes and results in a wide spectrum of antimicrobial activities ... effective against both aerobic and anaerobic bacteria for treatment of numerous infectious conditions in medicine and dentistry, very often with striking success [1] Different compounds of silver and

Ngày tải lên: 15/01/2020, 11:50

... PBS, and resuspe-nded in 100 μL of binding buffer Subsequently, cells were incubated with 5 μL of annexin V-FITC and PI, followed by incubation for 15 min at room temperature in the dark The stained ... oncogenes and tumor suppressor genes Aberrant expressions of cyclin D1 and cyclin E, downregulations of p16 and p27, and mutations of Rb and p53 have been frequently observed in several types of cancers ... mutated in most cancers and mutant p53 protein promotes malignant transformation It was found that accumulation of mutant p53 was closely related to the grading of bladder cancer and the progress of

Ngày tải lên: 15/01/2020, 20:51

... Trang 1Efficacy of photochemical internalisation using disulfonated chlorin and porphyrinphotosensitisers: an in vitro study in 2D and 3D prostate cancer models Alejandra Martinez de Pinillos ... tested in a range of human and non-human bladder cancer cell lines [34] with a view for using PCI for treatment of tumours in the bladder, which underlines the interest in urological applications of ... content, and all legal disclaimers that apply to the journal pertain. Trang 2Efficacy of photochemical internalisation using disulfonated chlorin and porphyrin photosensitisers: an in vitro study in

Ngày tải lên: 24/11/2022, 17:47

... less influ-enced by inflammation Moreover, increasing cell density and increasing concentrations of TNFα and IFNγ resulted in the secretion of more VEGFA, FGF2, and L-kynurenine, empha-sizing ... Effect of Cell Seeding Density and Inflammatory Cytokines on ASCs Cytokine Secretion We assessed whether increases in cell density led to changes in ASCs secretion of L-kynurenine, a metabolite of ... Trang 1Effect of Cell Seeding Density and Inflammatory Cytokineson Adipose Tissue-Derived Stem Cells: an in Vitro Study Panithi Sukho1,2,3 &Jolle Kirpensteijn1,4&Jan Willem Hesselink1&

Ngày tải lên: 24/11/2022, 17:54

... reported in 1964 by Rosenberg [1], and its anti-cancer activity in 1979 Cisplatin is routinely employed for the treatment of testicular and ovarian cancers and is being increasingly used against ... molecular mechanisms and activated signaling pathways, such as the protein kinase B/ mammalian target of rapamycin (Akt/mTOR) which is also implicated in Cisplatin resistance in human ovarian cancer ... (IV) prodrugs in inducing anti-cancer activity to Cisplatin resistant and sensitive ovarian cancer cell lines and showed that the uptake of Cisplatin is partially dependent on Ctr1 transporter,

Ngày tải lên: 21/09/2020, 02:01

... the interaction partners of proteins, we expanded our protein interaction network to include Table 1 Changes in the expression of individual isoforms of the Androgen Receptor in our cell line ... occurring as androgen resistance evolves over time Our aim is to understand biological network profiles of transcriptomic changes occurring during the transition to androgen-resistance and to ... signalling through ERK was in fact reduced in androgen-independent PCa [27], consistent with our findings here Discussion Here, we have described anin vitro model of the devel-opment of androgen insensitivity

Ngày tải lên: 22/09/2020, 23:34

... improving plant growth and suppressing disease However, there are few reports focusing on these aspects in India Certain isolates are known to produce auxins, cytokinins and vitamin B12 providing ... which is poor in nutrients and exposed to sun, wind and rain In contrast to phyllosphere organisms, the rhizospheric microbes occur in the below-ground area and remaining in a dark and moist environment, ... Methylobacterium strains CBMB20 and CBMB110 in the presence of L-tryptophan and obtained 2.33 and 4.03 μg/ml respectively after 5 days of inoculation Measurement of ACC-deaminase activity All

Ngày tải lên: 27/09/2020, 13:22

... mannitol aerosols exiting the nasal cannula and measured by laser included in the table to show the influence of leucine The D50, D90and span of Man+Leu increased slightly with in-creasing DFR However, ... DFR and IFR were independent of each other because a patient’s breathing is independent of the air coming out of the nasal cannula To minimize the aero-sol loss in the gap between the cannula and ... our findings in healthy human subjects Investigating subject-specific deposition in humans would be relevant as well The topography of the nasal airway can be accurately determined with an acoustic

Ngày tải lên: 23/10/2022, 15:54

... flavanones pinocembrin and pinostrobin, which were contrary, only small amounts of the main birch-bud flavanone sakuranetin was present in poplar-type propolis Sakuranetin and another flavanone, ... suppres-sion of the endogenous microflora in these bees, the almost inevitable pollution of beekeeping products with medicinal residues, and the appear-ance of bacterial resistappear-ance (Lodesani and ... University of Technology, 17-200, Hajnówka, Poland 2 Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland 3 Department

Ngày tải lên: 04/12/2022, 15:01

... normalizing the transfections Transient transfections using Lipofectamine LTX and PLUS reagent (Invitrogen) were performed according to the manufacturer's instructions Eight hours after transfection, ... siRNA (Stealth RNAi, Invitrogen) using Lipofectamine RNAiMAX (Invitrogen) ac-cording to the manufacturer's instructions After 8 h of transfection, the culture medium was changed to osteogenic ... V-ATPase were determined using 5μg/mL of anti-Wnt3a (Santa Cruz, CA), anti-Runx 2 (Santa Cruz), anti-β-catenin (BD Bioscience, CA), anti-V-ATPase (Santa Cruz), and anti-GSK-3β antibodies (Enzo Life

Ngày tải lên: 02/11/2022, 11:35

... domain linked to a glycosyl- phosphatidylinositol anchor showed no significant change in Table 1 Effect of anti-CD47/IAP, anti-TSP-1, and anti-SIRPα on mechanically induced changes in human articular ... engagement of α5β1 integrin and CD47/IAP [41]. It has been speculated that the five membrane-spanning segments of CD47/IAP and the two membrane-spanning domains of the heterodimeric integrin form an ... becoming increasingly clear that it has important roles in regulation and modulation of integrin signalling [14,17-21]. CD47/IAP is found in association with αVβ3 and other integrins [10-13]. Antibodies

Ngày tải lên: 09/08/2014, 10:22

... activities, including anti-viral [3], anti-tumor and anti-proliferative activity [4,5], this class of compounds revealed an anti-inflammatory activity in vitro by inhi-biting the generation of a superoxide ... anion in primed leucocytes, showing an inhibitory effect on the chemo-taxis of human peripheral blood neutrophils in vitro [5,6] An acute in vivo anti-inflammatory activity of this compound in ... showed an inhi-bition of the IL-6 and IL-8 expression induced by inflammatory cytokines concomitant to an enhance-ment of the COX-2 expression The possible pathways involved in inflammation and

Ngày tải lên: 12/08/2014, 17:21

... resistance to FLT3 inhibitors in MV4-11-R 56 3.3.3 Identification of enhanced activation of STAT pathways and overexpression of survivin in the resistant lines 58 3.3.4 Upregulation of survivin in ... Technology (CST, Danvers, MA); anti-Actin, anti-p21, anti-p27, anti-p53, anti-CDK2, and anti-CDK4 from Santa Cruz Biotechnology (Santa Cruz, CA) Rabbit anti-human c-Mos oncoprotein polyclonal antibody ... applications of ABT-869 will greatly benefit from better understanding of the molecular mechanism of the compound in sole or (N-[4-(3-amino-1H-indazol-combination therapies both in vitro and in vivo

Ngày tải lên: 11/09/2015, 09:06

... Trang 1   IN VITRO AND IN VIVO STUDY OF VITAMIN E TPGS COATED IMMUNOLIPOSOMES FOR SUSTAINED AND TARGETED DELIVERY OF DOCETAXEL ANANDHKUMAR RAJU (B.TECH, ANNA UNIVERSITY, INDIA) A ... Trang 31may also be involved Most of the time, multiple cases of cancer in a family are just a matter of chance Having more than two drinks each day for many years may increase the chance of ... 1998) While vinca alkaloids such as vindesine and vinorelbine can inhibit the assembly of microtubule by reducing the rate of tubulin addition, taxanes have the opposite effect, inhibiting the disassembly

Ngày tải lên: 09/10/2015, 11:18

... or antagonistic effects on human cancer cell lines when given together, an in vitro investigation would be conducted to examine the effects of COL-3 and tamoxifen, in single agent or in combination ... critical analysis Much of the drug interaction in vitro in the past has been interactions between drug and drug, and drug and fluid in intravenous infusions Newer work has concentrated on interactions ... drug-drug interactions Interactions involving renal excretory mechanisms can have important clinical implications in terms of patient mortality and morbidity Molecular mechanism of renal drug transport

Ngày tải lên: 09/10/2015, 11:24

... Trang 1Application of a Human Bone Engineering Platform to an In Vitro and In Vivo Breast Cancer Metastasis Model Verena Maria Charlotte Reichert, MD Faculty of Built Environment and Engineering, ... microscopy images to determine invasiveness levels of integrin alpha 2, 3, 5, v and beta 1and 3 Trang 18Chapter 5 and hOBs 179 in 3D 184 qRT-PCR analysis of 2D and 3D cultures 185 injections as well ... Osteomimicry of breast cancer cells 26 2.9 Osteolytic bone metastasis 27 2.10 Models investigating bone-cancer cell interaction in vitro 29 2.11 Models investigating bone-cancer cell interaction in

Ngày tải lên: 07/08/2017, 15:52

... type II and aggrecan core protein was steadily expressed in fibrin/PLGA and PLGA Interestingly, suppression of collagen type I was observed in fibrin/PLGA and PLGA at weeks and weeks β-actin gene ... the design of the study and conceived of the study GK participated in the design of the study, conceived the study and drafted the manuscript All authors read and approved the final manuscript ... explanation in relation to the significant reduction of cell proliferation in fibrin/PLGA hybrid construct by 1.37-fold by the end of 21 days of in vitro culture Macroscopic observation of in vitro...

Ngày tải lên: 20/06/2014, 01:20

... immunofluorescence and 3,3-diaminobenzidine disclosure) for aggrecan and the staining intensity increased with time post injection (Figures and 5b) Aggrecan matrix staining within the vicinity of ... within a small number of cells in monolayer culture Following injection into NP tissue explants, MSCs stained strongly positive for type II collagen and matrix staining in the vicinity of the injected ... X collagen and Alizarin red staining, and participated in interpretation of the data AJF participated in interpretation of the data and contributed to the preparation of the final manuscript JAH...

Ngày tải lên: 09/08/2014, 01:22

... TH and JR participated in gene expression data processing and in study design and coordination CK and MS conceived the study and participated in its design and coordination All authors read and ... oxidant injury [40] and inducing apoptosis [41] Besides ADORA2A, the expression of COX-2 as an important pharmacological target gene of inflammation was induced The formation of prostaglandins ... categorized into six rheumatoid arthritis-relevant groups and are listed with accession number, mean fold change in expression and mean signal intensity (generated by GCOS and RMA) Annotation of mean...

Ngày tải lên: 09/08/2014, 10:22

... modulating their expression or intrinsic adhesive functions [18] PMNs maintain several of these receptors in an inactive state during transit in the bloodstream and extracellular fluids, and only ... 92 ± 3% and 94 ± 5% inhibition, respectively These findings may suggest a direct action of these compounds on membrane structures and/or on CAMs, rather than through inhibition of an intracellular ... and interference with receptor activation Several groups of pharmaceutical agents in use clinically interfere with the function of CAMs either directly or indirectly Many clinical trials of anti-adhesion...

Ngày tải lên: 11/08/2014, 08:21