adult neural stem cells identity and regulation

high glucose induces apoptosis and suppresses proliferation of adult rat neural stem cells following in vitro ischemia

... performed flow cytometry Figure 1 Establishment of an adult neural stem cell in vitro model of ischemia (a) The identification of adult neural stem cells throughout different passages Immunocytochemical ... NSCs following anoxia, and to test whether high glucose affects NSC recovery from ischemic injury Results: In this study, we used immortalized adult neural stem cells lines and we induced in vitro ... reports have shown that endogen-ous and transplanted neural stem cells (NSCs) can be activated by cerebral ischemia and take part in the regen-eration of neural function [2,3] However, some basic

Ngày tải lên: 02/11/2022, 11:35

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fasudil stimulates neurite outgrowth and promotes differentiation in c17 2 neural stem cells by modulating notch signalling but not autophagy

... neurite outgrowth in neural stem cells (NSCs) Methods: Changes in cell morphology were imaged under a light microscope, and neurite-bearing cells were counted Cell viability and the necrosis rate ... differentiation in C17.2 cells through modulating Notch signalling but not autophagy Materials and Methods Cell culture and reagents The C17.2 cell line, which is composed of neural stem cells that were ... cytotoxic to the C17.2 cells in our research Fasudil promoted C17.2 cell to differentiate into neuronal cells and astrocytes C17.2 cells differentiated into neuronal cells and astrocytes as detected

Ngày tải lên: 02/11/2022, 10:39

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single cell transcriptomic analysis defines heterogeneity and transcriptional dynamics in the adult neural stem cell lineage

... Heterogeneity and Transcriptional Dynamics in the Adult Neural Stem Cell Lineage Graphical Abstract Highlights d Single-cell RNA-seq to characterize adult neural stem cell populations d Machine learning and ... strategies, and different neurogenic niches These data provide a resource for the field and contribute to an integrative understanding of the adult NSC lineage. INTRODUCTION Populations of neural stem cells ... young adult (3 months old) GFAP-GFP male mice and stained with markers of NSC identity and (A) FACS scheme for the enrichment of astrocytes, qNSCs, aNSCs, and NPCs from the SVZs of adult mice and

Ngày tải lên: 04/12/2022, 16:18

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báo cáo khoa học: " Capturing Alzheimer’s disease genomes with induced pluripotent stem cells: prospects and challenges" pps

... human embryonic stem cells Stem Cells 2011, 29:802-811. 45 Marchetto MCN, Carromeu C, Acab A, Yu D, Yeo GW, Mu Y, Chen G, Gage FH, Muotri AR: A model for neural development and treatment of ... pluripotent stem cells Cell 2010, 143:527-539. 46 Pruszak J, Ludwig W, Blak A, Alavian K, Isacson O: CD15, CD24, and CD29 define a surface biomarker code for neural lineage differentiation of stem cells ... Carson CT: Cell-surface marker signatures for the isolation of neural stem cells, glia and neurons derived from human pluripotent stem cells PLoS One 2011, 6:e17540. 48 Funato H, Yoshimura M, Yamazaki

Ngày tải lên: 11/08/2014, 12:21

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cancer stem cells theories and practice_part1 pps

... Leukemic Stem Cells to Overcome Drug Resistance 401 Miaorong She and Xilin Chen Cancer Stem Cells and Chemoresistance 413 Suebwong Chuthapisith Cancer Stem Cells in Drug Resistance and Drug Screening: ... population of drogen-insensitive cells that survive [androgen ablation therapy] and can expand in the absent of androgen … Since normal adult prostate stem cells (PSCs) are androgen-insensitive, it is ... transformation of normal stem cells into cancer stem cells,” but it might also be a consequence of long-term culture in vitro and not an intrinsic characteristic of stem cells On the other hand, “Aft er