adipogenic differentiation of human mesenchymal stem cells

... human stem cells cannot automatically be considered to behave in a similar way to other adherent cells lines This is because with human stem cells it is the cell itself that forms the basis of ... 10.6 cells/bead; b day 2, 10.7 cells/bead; c day 3, 6.2 cells/bead; d day 4, 9.5 cells/bead; e day 5, 19.7 cells/bead; f day 7, 52.1 cells/bead; g day 8, 67.6 cells/bead; h day 9, 98.4 cells/bead; ... of human stem cells is not a trivial exercise Indeed, expanding human stem cells in culture is currently more of an art than a science so much so that some laboratories cannot or do not grow human

Ngày tải lên: 02/11/2022, 10:41

... direct differentiation of human adipose stem cells into functional hepatocytes Jieqiong Huang*, Xinyue Guo*, Weihong Li & Haiyan Zhang The generation of hepatocytes that are derived from human ... presented in the cells of four groups The percentage of cells with LDL partiparti-cles in cells of Chir99021 at day 20 was relatively higher than other groups, which resembles the human hepatocytes ... identified to control the fate of mesenchymal stem cells (MSCs)29 However, little is known about the role of Wnt and its downstream intracellular pathways in the differentiation of definitive endoderm

Ngày tải lên: 19/11/2022, 11:45

... Recently the use of stem cells in regenerative medicine has gained momen-tum thanks to their capacity to differentiate into multiple lineages2,3 Human mesenchymal stem/stromal cells (hMSCs) can ... chondrogenic, osteogenic and adipogenic differentiation3, among others, and are not asso-ciated to the ethical concerns of other stem cells like embronic ones hMSCs differentiation has been reported ... the differentiation of hMSCs in certain areas of the construct, gradients of growth factors27 and material stiffness28 were generated To the best of our knowledge, no studies have linked the stem

Ngày tải lên: 04/12/2022, 10:32

... and adipogenic differentiation of human adipose derived stem cells E M Morandi1, R Verstappen1, M E Zwierzina2, S Geley3, G Pierer1 & C Ploner1 The fate of human adipose tissue stem cells ... induction of p21Cip1 in ITGAV- and ITGA5-KD cells Loss of ITGAV promotes adipogenic differentiation of ASC Previous studies have already shown that expression of ITGA5 counteracts adipogenic differentiation34 ... impact of RDG-motif signaling on adipogenic differentiation of ASCs via ITGA5 and ITGAV. In regenerative medicine, the exertion of influence on cell viability and in-vivo differentiation is of great

Ngày tải lên: 04/12/2022, 14:56

... the bone marrow (mesenchymal stem cells, hematopoietic stem cells) and (2) embryonic stem and embryonic germ cells derived from discarded human embryos and germ line stem cells. Adult stem cells can ... lineage specific differentiation of hES cells is mandatory. 1.5 Human Embryonic stem cells As the name suggests, the human embryonic stem cells are derived from the early embryo. These cells have ... and pluripotent stem cells that can give rise to representative of all three germ layers. Two important sources of stem cells available for regenerative therapy include (1) the adult stem cells from

Ngày tải lên: 09/10/2015, 11:24

... Introduction 1.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells Embryonic stem (ES) cells are isolated from the inner cell mass (ICM) of embryos of blastocyst ... Culture of human ES cell line 2.1.1 Preparation of feeder cells 2.1.2 Maintenance of human ES cells 2.1.3 Preparation of embryoid bodies 2.2 Culture of NCCIT cell line 2.3 Preparation of shRNA ... completion of my research Trang 31.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells 1.1.2 Regulatory networks and transcription factors in human ES cells

Ngày tải lên: 16/10/2015, 11:58

... and toxicity of O6 -methylguanine, and of MGMT, responsible for the removal of the O6-methyl adduct In fact, the lack of ex-pression of one of MMR components and/or the presence of high MGMT ... densities (O D.) of the protein of interest and those of tubulin The results are representative of one out of two experiments with similar results SJ: SJGBM-2 Panel C Analysis of MGMT activity ... activity was evaluated as fmol of32P-NAD+/μg of protein MGMT activity assay and bisulfite sequencing analysis of MGMT promoter methylation Cells (1×106) were lysed in 0.5 ml of a buffer containing 0.5%

Ngày tải lên: 05/11/2020, 01:28

... promiscuity of RAR isotype signalling the use of isotype-selective ligands (de Lera et al, 2007) in the context of wild-type cells appeared to us superior to the use of RAR isotype-deficient cells, ... implication of other RXRa heterodimers in ATRA-induced F9 cell differentiation has been reported (Chiba et al, 1997a) Highly dynamic binding of RXRa–RARc during differentiation Temporal analysis of RXRa ... the retinoid-induced differentiation ofF9 embryonal stem cells by integrative genomics Marco A Mendoza-Parra, Mannu Walia, Martial Sankar1and Hinrich Gronemeyer* Department of Cancer Biology, Institut

Ngày tải lên: 24/11/2022, 17:40

... chondrogenic differentiation of hMSCs and degradation of the hydrogels [21,34,35] Human mesenchymal stem cells (hMSCs) have been shown to benefit from the presence of fibronectin in the early stages of ... effect of RGDS peptide release In effect, the presence of the MMP7–cleavable moieties in the hydrogels and the action of encapsulated cells via the release of MMP7 enables the release of RGDS ... ng/mL recombinant human MMP7) Release of the RGDS peptide from the hydrogels was evaluated over time in the absence of cells using cystamine–FITC as a model substrate in place of the RGDS peptide,

Ngày tải lên: 24/11/2022, 17:41

... undifferentiated mesenchymal cells, which retain the ability to differentiate into mature cells Induced pluripotent stem cells have been developed from various cell sources, including dental pulp-derived stem ... Odontoblastic differentiation and the close cell signaling examination of DPSC differentiation were determined using western blotting Results: Hyaluronan induced expression of the odontoblastic differentiation ... Dental pulp stem cells (DPSCs) are present in human dental pulp, even in adult pulp, as clonogenic and highly proliferative cells obtained after enzymatic disaggrega-tion [2] These cells harbor

Ngày tải lên: 04/12/2022, 10:38

... number of CDCs for experiments, regardless of age To characterize the phenotype of CDCs, the percentage of cells positive for CD90 and CD105, which are validated markers of mesenchymal stem cells14, ... the function of cardiac stem cells In addition, these factors do not necessarily influ-ence the quantity and quality of stem cells uniformly For example, although hematopoietic stem cells from ... determinant of the quantity and quality of CDCs Senescent cells accumulate in tissues with advancing age Tissue-specific stem cells, for example those of the hematopoietic and musculoskeletal system

Ngày tải lên: 04/12/2022, 14:53

... MSCs A num-ber of previous studies involving intra-articular injection of exogenous cells have observed varying levels of car-tilage repair coupled with low numbers of engrafted cells at the injury ... /Sca-1+ The cells were cultured at 2% oxygen, which the authors suggested enhanced the differentiation potential of the cells The authors also observed that a single injection of 10,000 cells, irrespective ... & Joint Health, University of Calgary, Calgary, AB, Canada 2University of Calgary, Department of Surgery, Calgary, AB, Canada 3University of Calgary, Department of Radiology, Calgary, AB, Canada

Ngày tải lên: 04/12/2022, 14:57

... and self-renewal of multipotent human intestinal stem cells (a) Expansion of single human duodenum organoid cells (passage 7) able to form new organoids (n¼ 3, mean±s.e.m.) (b) Human duodenum organoids ... effects of small molecule WNT agonists on the multilineage differentiation capacity of human mesenchymal stem cells Tissue Eng Part A 22,1264–1273 (2016) 26 Huch, M et al Long-term culture of genome-stable ... expansion of undifferentiated ESCs (Fig 2d), and higher proliferation of human duodenum organoid cells than purified Wnt3a (Fig 2e) To specifically assay for the expansion of organ stem cells, we

Ngày tải lên: 04/12/2022, 15:06

... differentiation of cultured human mesenchymal stem cells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis of human mesenchymal ... Würzburg References Kolf CM, Cho E, Tuan RS: Mesenchymal stromal cells Biology of adult mesenchymal stem cells: regulation of niche, selfrenewal and differentiation Arthritis Res Ther 2007, 9:204 ... insight: adult mesenchymal stem cells for osteoarthritis therapy Nat Clin Pract Rheumatol 2008, 4:371-380 Estes BT, Wu AW, Guilak F: Potent induction of chondrocytic differentiation of human adipose-derived...

Ngày tải lên: 09/08/2014, 14:22

... vitro differentiation of size-sieved stem cells into electrically active neural cells Stem Cells 2002, 20(6):522-529 Tzeng SF, Tsai MJ, Hung SC, Cheng H: Neuronal morphological change of size-sieved ... promote differentiation of human mesenchymal stem cells into insulin producing cells J Biomed Mater Res A 2008, 86(4):1097-1105 Chao KC, Chao KF, Fu YS, Liu SH: Islet-like clusters derived from mesenchymal ... 53(7):1721-1732 Page 10 of 10 doi:10.1186/1423-0127-17-56 Cite this article as: Lin et al.: Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through...

Ngày tải lên: 10/08/2014, 05:21

... NANOFIBERS FOR OSTEOGENIC DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS NGUYEN THI HIEN LUONG (B.Eng., Ho Chi Minh city University of Technology, Vietnam) A THESIS SUBMITTED FOR THE DEGREE OF ... Nguyen, Susan Liao, Seeram Ramakrishna and Casey K Chan Role of Nanofibrous Structure in Osteogenic Differentiation of Human Mesenchymal Stem Cells with Serial Passage Nanomedicine 6(6): 961-974, 2011 ... Ramakrishna Electrospun Poly(L-lactic acid) Nanofibres Loaded with Dexamethasone to Induce Osteogenic Differentiation of Human Mesenchymal Stem Cells Journal of Biomaterials Science, Polymer Edition...

Ngày tải lên: 09/09/2015, 10:07

... expression pattern of undifferentiated and differentiated human embryonic stem cells Stem Cells Dev 2007, 16(6):1003-1016 Page 15 of 17 (page number not for citation purposes) Journal of Translational ... The tested adult cells were Human Microvascular Endothelial Cells (HMVEC), Human Umbilical Vein Endothelial Cells (HUVEC), Umbilical Artery Smooth Muscle Cells (UASMC), Normal human astrocytes ... 13 The expression of miR-92 has been reported in human embryonic stem (ES) cells [16,26], mouse ES cells[ 20] or Page 12 of 17 (page number not for citation purposes) Journal of Translational Medicine...

Ngày tải lên: 18/06/2014, 15:20

... culture of ICM cells with stem cell-like morphology from human blastocysts (Fig 1.1A) though cultures failed beyond passages The first long-term culture (4-5 months) of human embryonic stem cells ... analysis of developmental events was made possible with the isolation of embryonic stem cells Extensive investigation of the properties and capabilities of these cells has led to the development of ... Mu ° – Degree x CHAPTER 1: GENERAL INTRODUCTION 1.1 Embryonic stem cells The isolation of mouse and human embryonic stem cells (ES cells) heralded a new era in regenerative medicine, raising hopes...

Ngày tải lên: 04/10/2015, 16:03

... the induction of MSC adipogenesis Myogenesis Most investigations of myogenesis in adult stem cells are based on a small population of skeletal muscle-derived stem cells, or satellite cells A recent ... is critical for self-renewal of human multipotent adipose-derived stem cells Stem Cells 2006, 24:2412-2419 Kleber M, Sommer L: Wnt signaling and the regulation of stem cell function Curr Opin ... homing of human mesenchymal stem cells at exposed sites but promotes their widespread engraftment to multiple organs: a study of their quantitative distribution after irradiation damage Stem Cells...

Ngày tải lên: 09/08/2014, 10:20

... Spontaneous differentiation of germ cells from human embryonic stem cells in vitro Human molecular genetics 2004, 13:727-739 29 Niwa H, Miyazaki J, Smith AG: Quantitative expression of Oct-3/4 defines differentiation, ... RT-PCR analysis of expression levels of hTERT and GAPDH, and (B) Real-time RT-PCR analysis of expression levels of DNMT1, DNMT3A and DNMT3B in primary human mesenchymal stem cells transfected ... of mesenchymal stem cell in vitro BMC cell biology 2006, 7:14 Hung SC, Chen NJ, Hsieh SL, Li H, Ma HL, Lo WH: Isolation and characterization of size-sieved stem cells from human bone marrow Stem...

Ngày tải lên: 10/08/2014, 05:21