a reliable tool for the analysis of cellular processes

... The area (b) reports details of the integrated network The area (c) is the canvas where the graph is drawn and can be manipulated The area (d) reports the operations that can be applied on the ... di Milano, Via Celoria 18, 20133, Milano, Italy Full list of author information is available at the end of the article networks, often carry complementary information each other, and often a source ... integration of nine organisms, the prediction of thousands of GO protein functions, and a easy-to-use graphical interface for the visual analysis, navigation and interpretation of the integrated networks...

Ngày tải lên: 25/11/2020, 12:44

... 1992 The ‘liberalization of the EUagricultural market’ indicates the fundamental reform of the CAP ana-in 2003 The energy tax act specifies the energy tax law enacted in 2006 The biofuel quota act ... situations sequentially take place in the actionarena and they are repeated until the stop criteria (final year)are met Agents adapt to the environment in each iteration Theadaptation mechanism ... annually the set-aside land quota depending on the state of the market The extension of the quota oscillated between 5% and 15% of the total agricultural area Farmers were allowed to culti-vate...

Ngày tải lên: 08/11/2022, 14:57

... Ca 2+ localization showed a gradual accumulation on the stigma, and further expansion toward the style and the ovary after anther dehiscence. At the post-anthesis phase, the Ca 2+ signal on the ... showed the accumulation of Ca/Sb precipitates in the vacuoles of the stigma cells as well as in the intracellular spaces between them. The stigmatic surface is the main place for signal exc hange ... Molecular de Plantas, Estación Experimental del Zaidín (CSIC), Profesor Albareda 1, 18008, Granada, Spain Full list of author information is available at the end of the article Zienkiewicz et al....

Ngày tải lên: 11/08/2014, 11:21

... significantly different cold-accli-mation capacities, we prove that mathematical model-ling of metabolism and validation by experimental data offers an attractive possibility for the study of complex ... or metabolic pathways other than carbo-hydrate pathways was calculated as the difference between net carbon uptake and changes in cellular car-bohydrate content The resulting surplus of carbon ... different pots in a random design before and after 1 day, 3 days and 7 days of exposure to 4C Samples were taken after a light period of 8 h At that stage, the aerial part of the plant is exclusively...

Ngày tải lên: 14/02/2014, 22:20

... negative when the substance on the right is a substrate of the reaction Then the arrow points backward, i.e away from the substance and there is a right-hand factor –a equal to)1 Influences of the ... the type of Graph 1 (these paths may contain parallel and antiparallel arrows) The sign of that element therefore depends on the both the sign and the magnitudes of these influence paths (see ... oscillations The main result of such an analysis is that oscillations arise if the parameter k3is the largest and the parameters k6and k7are the smallest in the system Oscillations in this system can indeed...

Ngày tải lên: 19/02/2014, 16:20

... Trang 1Modeling the three-dimensional structure of H+-ATPaseProposal for a proton pathway from the analysis of internal cavities Olivier Radresa1, Koji Ogata2, Shoshana Wodak2, Jean-Marie ... in the cytoplasmic domain The cavities identified in this domain trace a clear path that starts in the center of the b-strands forming the Rossman fold and leads to the membrane domain at the ... alignment The sequences of Neurospora crassa H+-ATPase (PMA1_NEUCR) and rabbit SERCA1a/ Ca2+-ATPase (ATCI_RABIT) were retrieved from the Swissprot database [22] Obtaining a correct sequence alignment...

Ngày tải lên: 23/03/2014, 21:20

... distally along the shoe), medial (greater medial than lateral wear at the heel and forefoot), which may indicate excessive pronation, or lateral (greater lateral than medial wear at the heel and forefoot), ... effect the position of the foot in the shoe, forefoot height was also measured. This measurement was taken at the level of both the first and fifth metatarsophalangeal joints and the average of both ... Central Page 1 of 12 (page number not for citation purposes) Journal of Foot and Ankle Research Open Access Research Development and evaluation of a tool for the assessment of footwear characteristics...

Ngày tải lên: 10/08/2014, 21:23

... study and was involved in the evalua-tion of the data as well as the writing of the manuscript All authors read and approved the final manuscript Trang 10Additional material AcknowledgementsThe authors ... uptake as a marker for DNA synthesis in activated cells of S phase was also included as test of more functional relevance An important key regulator of the apoptotic pathway such as caspase-3 was ... decrease of cell viability as compared to untreated controls Statistical analysis Results of viability testing and RT-PCR analyses as well as the data for human cell lines are shown as mean values...

Ngày tải lên: 12/08/2014, 15:20

... SO as part of a database schema ensures that such ques-tions 'mean' the same thing in different databases SO also greatly facilitates the automatic validation of annota-tion data, as the relaannota-tionships ... functional/heteromerous/separable A sheep is a part_of a flock. A read is a part_of a contig. place_part_of area Not functional/homomerous/not separable England is a part_of Britain. feature_part_of ... features in SO and SOFA are richer than those of the Feature Table [12] of the three large genome databanks: GenBank [1], EMBL [13] and the DNA Data Bank of Japan (DDBJ) [14] The Feature Table...

Ngày tải lên: 14/08/2014, 14:21

... function of the cells
Assays that measure the cellular DNA quantity The determination of DNA concentration is a reasonable indicator of cell number, since the levels of DNA and RNA in cells are ... reader analysis, 2 to enhance the formation of collagen matrix on the fibroblast culture, 3 to develop collagen quantification assay that is based on a non-radioactive method and suitable for a microplate ... be an indication of cell viability There are several metabolic based assays available in the market, MTT assay being the commonly used This assay is based on the reduction of tetrazolium salts...

Ngày tải lên: 09/10/2015, 11:07

... comminution cannot be achieved with the means available in the laboratory, the use of larger sample amounts for analysis (scaling up) and/or the use of Ultra-Turrax during the first extraction step may ... sample fat will form an additional layer into which analytes may partition and get lost The extent of losses depends on the amount of lipids in the sample as well as on the polarity of the analytes ... matrix of the samples to be analyzed and should not contain any detectable residues of the analytes of interest) The blank sample is treated the same way as any other sample, but no ISTD is added...

Ngày tải lên: 06/10/2016, 09:38

... of a simply supported slab is met two days after casting The accuracy of the assessment methodologies are then evaluated and from this evaluation a decision-making tool for the striking of formwork ... 3.2 The test days of interest for striking a slab are 2, 3, and 4 The test day ages of 7 and 28 are standard test day ages and the test day ages were brought out to 56 days to investigate the late ... Area Technical Manager, for assisting with all matters on site in Clonee and to Barry and Gary in the lab for the help with the testing, especially on Saturdays and on Christmas Eve A word of...

Ngày tải lên: 03/11/2019, 10:12

... Chapter Immunoelectron Microscopy: A Reliable Tool for the Analysis of Cellular Processes 65 Ana L De Paul, Jorge H Mukdsi, Juan P Petiti, Silvina Gutiérrez, Amado A Quintar, Cristina A Maldonado ... number of species, a wide, versatile range of secondary antibodies are available to detect any primary antibody An additional advantage of using secondary antibodies is that several secondary antibody ... channels at the plasma membrane using ICC, only the small extracellular parts of the ion channel are available for antibody binding Such extracellular loops are relatively well-conserved between channels...

Ngày tải lên: 28/06/2014, 08:20

... A A A P – pS P P P P A A – P A S A A A P A A A A A A A mK K K K K aK – A A A A A A A K K K K K K amK A A P A A A R K K K K K K pS A A A A A A pS T T T T T T T K K K K K K A K K K K K K K K K K ... variation acting as a marker for a particular phenotype However, the main aim of Sarg et al [34] was to demonstrate the use of a particular chromatography technique, rather than to firmly establish ... human and mouse were taken from [43], and the data for chicken were taken from [44] Chicken H101 H110 H102 H103 H11L H11R H5 a- S a- S a- S a- A a- S a- A a- pT T T T pT T pT pS A A A A A A A A A A A P...

Ngày tải lên: 18/02/2014, 08:20

... biodegradability of superparamagnetic core-shell nanoparticles Biomaterials 2010, 31:1316-1324 19 Hatanaka S, Matsushita N, Abe M, Nishimura K, Hasegawa M, Handa H: Direct immobilization of fluorescent ... 14 Lacava LM, Garcia VAP, Kuckelhaus S, Azevedo RB, Lacava ZGM, Silva O, Pelegrini F, Gansau C, Buske N, Morais PC: Magnetic resonance and light microscopy investigation of a dextran coated magnetic ... JC, Gazeau F: Intracellular uptake of anionic superparamagnetic nanoparticles as a function of their surface coating Biomaterials 2003, 24:1001-1011 57 Wilhelm C, Gazeau F: Universal cell labelling...

Ngày tải lên: 11/08/2014, 00:22

... TCC AAC TGC AGA GCC atg tcc cct ata cta- 3’ 5’-GGT GGT CTG CAG tca gtc acg atg cgg-3’ 19 pT-Rex-DEST30-intein/EGFP 5’-GGGG ACA AGT TTG TAC AAA AAA GCA GGC TTC GAA GGA GAT AGA ACC ATG GCT ATC ... minimal modifications to the target protein, apart from the introduction of a few extra amino acid residues at the N-terminus of the target protein We have shown that the strategy may be readily applied ... glutathione was conjugated with Cy3-NHS (Amersham Pharmacia, USA) for 1h in 0.1M NaHCO3, pH according to the manufacturer’s protocol and purified through a NAP-5 column (Amersham Pharmacia, USA)...

Ngày tải lên: 08/11/2015, 16:30

... [40] The mean of the individual ratios of the two measured AUC are then multiplied by 100 to derive the foods GI value Over the years, GI values for a wide THEORETICAL BACKGROUND range of carbohydrate ... indicated a prevalence of 7.2% among adults aged between 18 and 79 years with an additional 2.1% of undiagnosed cases [65] Compared to data from the German National Health Interview and Examination ... cytokines and macrophages, inflammatory signaling pathways can also be activated intracellularly through metabolic 19 THEORETICAL BACKGROUND stress [118]: the functional capacity of the ER can be overburdened...

Ngày tải lên: 25/11/2015, 14:52

... Osaka, Kobe, Hiroshima, and others Therefore, it is considered that a large amount of pollutants runs into the sea from the urban as well as industrial areas As the area around the Seto Inland ... around Matsuyama for multipurpose usage such as agriculture and urban life There are citrus groves around the urban area of Matsuyama, which occupy more than 14% of the whole area Therefore, it ... increased amount of SS to the increased amount of Chl .a was higher than that of the algae Increased amount of SS were higher in the cases of high nitrate concentration - 117 - Journal of Water and...

Ngày tải lên: 05/09/2013, 10:17

... find out the similarities and differences between the syntactic features of ESVs and VSVs - Analyzing data of ESVs and VSVs in terms of the semantic features and making a contrastive analysis to ... 22 Table 4.46 A Summary of the Meaning Nuances of SUGGEST Table 4.48 A Summary of the Comparison of the Meaning and Their Vietnamese Equivalents Verb English Meaning Nuances Vietnamese Equivalents ... of any status can have good ideas to suggest - More relate to the moral or emotional aspects Table 4.49 The Semantic Specification of the Groups of SVs Table 4.47 A Summary of the Meaning Nuances...

Ngày tải lên: 26/11/2013, 13:17

... beliefs, arts, morals, law, custom, and any other capabilities, and habits acquired by man as a member of a society” ♦ Relation of Culture and Language The relationship between language and culture are ... below Authentic Data I have had a survey at a foreign language class at Quang nam university In which, the learners are asked to translate some EFLSs into Vietnamese The result shows that the learners ... process, maybe culture or habits of using languages of Vietnamese translators It means that the translation process is a complicate process Therefore, the learners and the translators of English may...

Ngày tải lên: 26/11/2013, 13:21