... reassembly stacking protein 55 (GRASP55) Further analysis revealed that GRASP55 associated with the cytoplasmic domain of both proteases and that the LLY573motif in the MT1-MMP intracellular ... GRASP55 using a mammalian two-hybrid (M2H) system Using this approach, we have identified the GRASP55 bind-ing site in the ICD of MT1-MMP, as well as the GRASP55 domains involved in the interaction ... using the MYC tag antibody Top black arrowheads indicate IgGs The bottom black arrowhead indicates a crossreaction (C) IB analysis of protein extracts prepared from the EGFP-negative (lane 1) and
Ngày tải lên: 18/02/2014, 04:20
... is advocating the rights of nations like Pakistan, Afghanistan, Saudi Arabia, Syria, Iran, and, yes, Iraq; and, rather than labeling them as evil, treating them with reason and decency A great ... agendas and bank accounts above the well-being of their nation Since at least Calvin Coolidge, there was nothing new in acting against the interests of the common people in order to aggrandize the ... Reagan’s cut all but eliminated the Pell Grants, and at a time when, according to Michael Boskin (Reagan and the Economy, 1987), “the personal savings rate… was at a 40 year low.” By claiming
Ngày tải lên: 06/03/2014, 06:21
Philosophy in a New Key A Study in the Symbolism of Reason, Rite, and Art
... elaborate 'ape-ways' into which a newcomer is graduallyacculturated, including among other patterns ways of usingavailable instruments for reaching and climbing, a sort ofrhythmic play or dance, and ... might arise, just as they occur in overt action; the white rat in a maze makes mistakes, and sodoes the trout who bites at a feather-and-silk fly In so com-plicated an organ as the human cortex, a ... objects as molecules and ether-waves—we really see onlythe fluctuations of a tiny arrow, the trailing path of a stylus, or the appearance of a speck of light, and calculate to the Trang 25"facts"
Ngày tải lên: 14/03/2014, 17:25
A walk in the Garden of Eden Genetic Trails into our African Past pptx
... state may claim that it, too, has an interest in this information – if it might result in reducing the escalating health care budget, for example Forensic DNA databases are being set up in many ... to all that, she is an efficient organiser who is not afraid of hard work She is playing an important role in furthering the aims of the AHGI Himla Soodyall is an enthusiast; a great human being, ... beenlaunched, primarily by the Americans, but soon joined by theBritish, the French, the Germans, and the Australians There werevery few human geneticists in South Africa at that time andmolecular
Ngày tải lên: 15/03/2014, 02:20
Once Upon A Time in the Italian West: The Filmgoers' Guide to Spaghetti Westerns
... thesurrounding area, including the rock formations at La Pedriza (in the GuadarramaMountains), the reservoir at Santillana and the landscape of Manzanares El Real Trang 19The Andalusian province of Almeria ... quarries atMagliana, the landscape of Manziana around Bracciano Lake, the Abruzzo NationalPark and the Nature Reserve at Tor Caldara The most distinctive spaghetti western locations were in Spain ... what amounts to a cameo role as Marisol, a Mexican peasant womanwith jet-black hair and Cleopatra eyeliner Koch was a popular actress in Europe atthe time, occasionally appearing in British and
Ngày tải lên: 15/03/2014, 18:39
A Plum In The Syrup pptx
... high achievement For example, an auto mechanic stands a better chance of earning more than a day laborer and an attorney stands a better chance of earning more than an auto mechanic The reason ... been said that there has been more information and reading material printed and published over the past 20 years then in all other previous years combined I cannot say with certainty whether or ... again, the law of attraction says there is no such thing According to the law of attraction the question ultimately lies in what is particular to this man’s internal energy to attract the winnings
Ngày tải lên: 16/03/2014, 21:20
Báo cáo khoa học: H2O2, but not menadione, provokes a decrease in the ATP and an increase in the inosine levels in Saccharomyces cerevisiae An experimental and theoretical approach pot
... at the indicated times, as described in Materials and methods. Trang 10H2O2evokes a decrease and an increase in the intracellularconcentration of ATP and inosine, respectively Searching for the ... disappearance of ATP or appearance of Ino were observed Theoretical approach – mathematical simulation of some metabolic pathways related to adenine nucleotide meta-bolism This was a theoretical approach ... 5¢–nucleotidase), E3 (AMP deaminase), E4(adenosine deaminase), E5(purine nucleo-side phosphorylase), E6 (adenylate kinase), E7 (adenosine kinase), E8(a hypothetical enzyme catalyzing two general and
Ngày tải lên: 17/03/2014, 10:20
Báo cáo Y học: Nuclear proteins that bind to metal response element a (MREa) in the Wilson disease gene promoter are Ku autoantigens and the Ku-80 subunit is necessary for basal transcription of the WD gene ppt
... nuclear extracts were incubated with avidin–agarose beads (Sigma) at 4C for 30 min to eliminate materials in the extracts that bind nonspecifically to the beads After removing the beads by filtration, ... molecular size marker (SM) are indicated at the left of each panel The purified protein bands are indicated by solid arrowheads. Fig 4 Microsequencing analysis of the MREa-binding proteins. (A) The ... was carried out for 30 min at 25C After elinimating by centrifugation denatured pro-teins generated during the binding reaction, the mixture was combined with 20 lL avidin–agarose beads and incubated
Ngày tải lên: 17/03/2014, 23:20
THE FIRST BANK OF THE UNITED STATES - A CHAPTER IN THE HISTORY OF CENTRAL BANKING pdf
... $100 Although reluctant to intercede in financial markets, Hamilton saw the need for intervention as the earlier financial bubble kept collapsing, credit was becoming less available, and the possible ... other banks to obtain shares in the First Bank and as the First Bank itself opened and began making loans and issuing its own banknotes In addition, Duer was borrowing heavily to pay for his investments ... opposition in some quarters Secretary of State Thomas Jefferson, for one, was afraid that a national bank would create a financial monopoly that would undermine state banks He also believed that creating
Ngày tải lên: 22/03/2014, 21:20
AMERICAN HERO-MYTHS. A STUDY IN THE NATIVE RELIGIONS OF THE WESTERN CONTINENT potx
... Hermeias, is but a transliteration of the Sanscrit Sarameyas, under which he appears in the Vedic songs, as the son of Sarama, the Dawn Even his character as the master thief and patron saint of the ... than others, and most of the American languages do so in a marked manner, by the broad grammatical distinctions they draw between animate and inanimate objects, which distinctions must invariably ... the Calendar Meaning of the Name The Myth of the Four Brothers Kukulcan's Happy Rule and Miraculous Disappearance Relation to Trang 7Quetzalcoatl Aztec and Maya Mythology Kukulcan a Maya Divinity
Ngày tải lên: 22/03/2014, 22:20
Episode 13 A wedding in the air docx
... Donatella Versace está por llegar de un momento a otro Tiene ideas maravillosas para mi traje para la boda ¡Hasta pronto! Sound of telephone receiver being put down MRS ROMERO Gracias, Antonio ANNIE ... have mail! Ah, and they’re all from your mother Shall I? HECTOR Yes please, do ANNIE What do they say? HECTOR ¿Cuántas damas de honor va a llevar Annie? ¿Diez o doce? Well, she says, “How many ... did you have a good day? NICK Gre-at! I saved London again Oh, and I was interviewed by Bridget Man, I thought she was going to ask me to marry her! Ha-ha! HECTOR Gre-at NICK OK, what’s up? HECTOR
Ngày tải lên: 23/03/2014, 00:20
Báo cáo khoa học: Identification of a domain in the a-subunit of the oxaloacetate decarboxylase Na+ pump that accomplishes complex formation with the c-subunit pot
... the biotin-binding domain in the C-terminal part [5] The b-subunit is an integral membrane protein with nine membrane-spanning a-helices and a fragment inserting into the membrane but not traversing ... contains the carb-oxyltransferase domain in its N-terminal portion and the biotin-binding domain in its C-terminal portion The c-subunit plays a profound role in the assembly of the complex It interacts ... more conservative than the mutation D509A the mutation D509A probably causes a conformational change in a-2-C resulting in a rearrangement of the binding surface which in turn allows another residue
Ngày tải lên: 23/03/2014, 13:20
History of Cuba; or, Notes of a Traveller in the Tropics pdf
... theassertion of the discoverers The island was politically divided into nine provinces, namely, Baracoa, Bayaguitizi, Macaca, Bayamo, Trang 22Camaguey, Jagua, Cueyba, Habana and Haniguanica At ... captain-general was established in 1589, and, with a succession of incumbents,the office has been maintained until the present day, retaining the same functions and the same extraordinarypowers The ... on the death of Ferdinand;afterwards called Ave Maria, in honor of the Holy Virgin Cuba is the Indian name [2] "Thus," exclaims the pious Arrati, "began that gathering of an infinite
Ngày tải lên: 24/03/2014, 03:21
Báo cáo hóa học: " Targeting the inflammation in HCV-associated hepatocellular carcinoma: a role in the prevention and treatment" pptx
... increased levels of aspartate transaminase, alanine transaminase, alkaline phosphatase, acid phosphatase, lactate dehydrogenase, gamma-glutamyltransferase, 5’-nucleotidase, bilirubin, alpha-fetoprotein, ... toll-interleukin-1 receptor domain-containing adaptor inducing IFN (TRIF) TRIF is an adaptor protein that is a critical component of the TLR3 signaling pathway By cleaving IPS-1 and inactivating ... Trang 1R E V I E W Open AccessTargeting the inflammation in HCV-associated hepatocellular carcinoma: a role in the prevention and treatment Giuseppe Castello1*, Susan Costantini1*, Stefania Scala2
Ngày tải lên: 18/06/2014, 16:20
Báo cáo hóa học: "Research Article Harnack Inequality for the Schrödinger Problem Relative to Strongly Local Riemannian p-Homogeneous Forms with a Potential in the Kato Class" potx
... local Riemannian p-homogeneous forms with a potential in the Kato class The first result in the case of Laplacian has been given by Aizenman and Simon [1] They proved a Harnack inequality for the ... Laplacian were defined in [3], and the case of (bilinear) Riemannian Dirichlet form was considered in [4,17] In [7] the Kato class was defined in the case of subellipticp-Laplacian and in [10] the ... is a measure in the Kato class and we prove a Harnack inequality (on balls that are small enough for the intrinsic distance) The main difference with respect to [7] is the proof of theL ∞-local
Ngày tải lên: 22/06/2014, 22:20
Báo cáo toán học: "A Discontinuity in the Distribution of Fixed Point Sums" pps
... enumerative situation in which thoughts of Trang 3discontinuities might be far from the mind of an investigator, yet they materialize in an interesting and important way A quick explanation for the ... trivial Not so, however • Permutations in which elements in the same cycle must have the same parity have a label-based constraint on structures Hence the replacement in (c) may not give a valid ... n−|S| labels for the rest of the structure and (c) replacing i by ai in D where [n] \ S = {a1 < a2 < }. At first, deciding the truth or falsity of (i) in a particular instance may seem
Ngày tải lên: 07/08/2014, 07:21
Báo cáo y học: "Is there a dysfunction in the visual system of depressed patients" pptx
... one can study the electrical and metabolic activity of the outer layers of the retina During the adaptation of the retina to dark, the amplitude of the EOG gradually decreases, reaching a nadir ... depression and just intensify or attenuate the clinical picture in the same way they affect normal mood e The internal coincidence theory, which basically focuses to the time of awakening Wehr and Wirz-Justice ... of the current study provide empirical data in order to assist in the understanding of the international literature and to explain the mechanism of action of therapies like sleep deprivation and
Ngày tải lên: 08/08/2014, 21:20
Báo cáo y học: " Initiation of bacteriophage T4 DNA replication and replication fork dynamics: a review in the Virology Journal series on bacteriophage T4 and its relatives" pptx
... mutational inactivation of T4 recombination proteins leads to the DNA arrest phenotype, characterized by a paucity of late DNA replication The additional inactivation of UvsW suppresses this DNA ... replication is blurred by the involvement of recombina-tion proteins in certain aspects of origin replicarecombina-tion Another example of overlap is the finding that repair of double-strand breaks ... sequence that is invaded could be at the other end of the same DNA molecule, since the infecting T4 DNA is terminally redundant, or it may be within the interior region of a co-infecting T4 DNA molecule,
Ngày tải lên: 11/08/2014, 21:21
Báo cáo y học: " Structural analysis of bacteriophage T4 DNA replication: a review in the Virology Journal series on bacteriophage T4 and its relatives" pptx
... [17]. The E. coli helicase loader, DnaC, is also a t wo domain protein. However, the C-terminal domain of DnaC is an AAA + ATPase related to DnaA, as revealed by the structure of a truncated DnaC ... loading of the ring shaped PCNA clamps onto duplex DNA is a conundrum; imagine a magician’ s linking ring s taken apart and r eassembl ed without an obvious point for opening. The clamp loa- ... composed of the gp41 hexameric helicase, the gp59 helicase loading protein, the gp61 primase, and the gp32 single stranded DNA binding protein. A.) the gp32 single-stranded DNA binding protein binds
Ngày tải lên: 11/08/2014, 21:21
Báo cáo sinh học: " A mutation in the MATP gene causes the cream coat colour in the horse" pot
... Reverse CAAATAAGTAGGCTTTGATG Reverse GTCCATCCAATGAGGTGGCTG Reverse GAGGTTAAAGGGCACAGTGT Reverse CCGCAGACGCTGTGATCACC Reverse Trang 6or Sau3A, and PCR amplification of the specific regions was performed ... MATP (AIM-1) Membrane-associated transporter protein (underwhite) CACAGGTTTTGGAGGTGCCC GAACATGACCTGGAATTCTG 0.95 / mouse MYO10 Myosin X GCCATCAAGATATTCAATTC GTCAGGATCTGCCAGCTGTA 0.92 / human were ... (HMS77) with a (TG)20N(GA)17GC(GA)5 repeat in the BAC clone containing MYO10 (Tab IV) On the contrary, no microsatellite was found in the BAC clones containing MATP. When amplified on a DNA panel of
Ngày tải lên: 14/08/2014, 13:21