5 apos end of expressed genes in zebrafish

... (20.95%) Trang 6the levels of four types of amino acids (proline, serine,threonine and GABA), but an increase in the levels of another four types of amino acids (lysine, valine, aspara-gine and ... process” (13.9%) In terms of molecular function, the genes were dominant in “catalytic activity” (31.4%),“binding” (24.4%), “ion binding” (15.5%), “hetero-cyclic compound binding” (13.5%) and “organic ... glucuronate interconversions 3 Pectate lyase 5, Pectinesterase 3, Pectinesterase 2 Phagosome 3 Tubulin beta-1 chain, Tubulin alpha chain, Tubulin alpha chain, Tubulin beta-4 chain Cysteine and methionine

Ngày tải lên: 26/05/2020, 23:48

... protein Four of them were clustered to profile 1, and 1 to profile 0 showing down-regulated trends Only 1 DEG belonging to profile 1 was annotated to auxin-induced protein AUX/IAA In the auxin ... up-regulated profiles, including 3 unigenes encoding ethylene receptor (ETR), 2 encoding ein3-binding F-box protein (EBF1/2), 3 encoding ethylene-insensitive 3b (EIN3) and 2 encoding ERF transcription ... components, including the abscisic acid receptor PYR1, protein phosphatase 2C, ABA responsive element-binding protein 2 and serine/ threonine-protein kinase SRK2E-like isoform 1 increased while that of

Ngày tải lên: 02/11/2022, 09:22

... spliced genes (A) Alternatively spliced unigenes (B) Alternative splicing types Fig 5 Venn diagram of the number of expressed genes (a) Venn diagram of the number of genes expressed in the normal ... capitula, in-cluding some important transcription factor genes (MYB, GRAS, and BTF3 genes), ubiquitin-conjugating enzyme genes, zinc finger protein genes, and many unannotated genes These genes may ... expressed only in the normal capitula, in-cluding 36 C2H2 genes, 6 bZIP genes, 5 bHLH genes, 5 MYB genes, 4 HB-other genes, 2 C3H genes, 2 E2F/DP genes, 2 GATA genes, 1 ERF gene, 1 HSF gene, 1 NF-X1

Ngày tải lên: 30/01/2023, 20:10

... was expressed in more than 50 cells, in 5–50 cells, in 1–4 cells, and in no cells per eye, respectively Immunohistochemistry and image capture Immunostaining was carried out against the retina ... capable of inducing spatial differentiation of expression Furthermore, by analyzing transgenic fish with a series of arrays consisting of the RH2-LCR and multiple upstream regions of the RH2 genes in ... occurred independently in the two superorders [3, 5], a portion of the RH2-LCR was highly conserved among them (Fig 1a, b) This is consistent with the RH2 opsin genes being expressed in the SDC in

Ngày tải lên: 27/03/2023, 05:16

... pro-posed [55-57] Evolutionary developmental biology of MADS box genes in a range of angiosperms has been instrumental in the development and testing of these models [reviewed in 58] and further ... Expression profiles of Actinidia flowering genes in mature plant organs Real-time RT-PCR analysis of the Actinidia flowering genes in the root, stem internode, leaf, flower and fruit of two kiwifruit ... 3LV Figure 5 Expression profiles of Actinidia flowering genes in normal and aberrant flowers Real-time RT-PCR analysis of the Actinidia flowering genes in the leaf and floral organs of A deliciosa

Ngày tải lên: 11/08/2014, 11:22

... Department of Applied Biology, PO Box 27, FIN-00014 University of Helsinki, Helsinki, Finland, 2 Finnish Graduate School in Plant Biology, PO Box 56, FIN-00014 University of Helsinki, Helsinki, Finland, ... School in Biosciences, PO Box 56, FIN-00014 University of Helsinki, Helsinki, Finland, 4 Horticultural Sciences Department, University of Florida, Gainesville, FL, USA and 5 Institute of Biotechnology, ... earlier [54], and putative SWN1 (SWINGER 1), FIE (FERTILIZA-TION INDEPENDENT ENDOSPERM), VRN1 (VERNALI-ZATION 1) and LHP1 (LIKE HETEROCHROMATIN PROTEIN 1) [19,55,56], which were found in this investi-gation

Ngày tải lên: 12/08/2014, 03:21

... Panax ginseng for all shared protein-coding genes Figure 4 Pairwise d N /d S of Nicotiana and Ipomoea vs Panax ginseng for all shared protein-coding genes Genes are ranked left to right by increasing ... retains the open reading frame, matK, con-tained within the intron of that tRNA A deletion within the trnV -CAU intron also reported in C reflexa [21], and similar to that seen in Orobanche minor, ... than that of Nicotiana tabacum (155,939 bp), largely through expan-sion of the IR region into the SSC region (fig 1) While the IR of Nicotiana barely extends into ycf1, the IR of Ipomoea includes

Ngày tải lên: 12/08/2014, 05:20

... euthanasia, and improving end-of-life care J Clin Oncol 2005, 23(27):6456-6458. 34. Goy E, Ganzini L: End-of-life care in geriatric psychiatry Clin Geriatr Med 2003, 19:841-856. 35. Lloyd-Williams ... at the end-of-life (Table 2) [31] Pain control One of the most common debilitating symptoms at the end-of-life is pain Pain control is an integral element of palliative care Because pain is a ... professional training, research, prioritizing pain control, and global awareness of palliative medicine[14-16] Palliative care is becoming increasingly valued in the age of modern medicine where technological

Ngày tải lên: 13/08/2014, 23:20

... in CA The 601 genes in Figure 3b explain 36.39% of the total variance in the first axis and 16.05% of that in the second axis The 220 genes in Figure 3c contribute to 17.02% of the variance in ... in blocks 3-5 are genes showing Trang 11interaction effects between the cell lines and vitamin D treatment with genes highly expressed in the hMSC-dlk1 cell line but without vitamin D treatment ... treatment Finally, at the bottom of Figure 3a (block 6), we have a small cluster of genes exhibiting the main effects of vitamin D which are up-regulated in both cell lines It is necessary to point

Ngày tải lên: 14/08/2014, 14:21

... development in zebrafish 52 3.6 RNA binding analysis by zebrafish Stau proteins 54 3.6.1 Zebrafish Staufen dsRNA binding domains bind mRNA in vitro 55 3.6.2 Zebrafish Staufen dsRBD-4 binds nanos1 ... and branching 113 Chapter 5: DISCUSSION 115 5.1 Functions of Staufen proteins in PGC development 115 5.1.1 Implications of Staufen proteins in mRNA binding and localization 115 5.1.2 Are ... vg1 mRNA in ovary total RNA 57 3.7 Functions of Staufen proteins in zebrafish 57 3.7.1 Specificity of dominant negative peptides to deplete Staufen in zebrafish 57 3.7.2 Specificity of antisense

Ngày tải lên: 12/09/2015, 08:18

... analysis of zebrafish Pin1 61 3.1.1 Characterization of zebrafish Pin1 61 3.1.2 Sequence alignment of Pin1 in various species 63 3.1.3 Expression pattern of zPin1 in zebrafish embryos 65 3.2.2 The interaction ... is inhibited by one of the most conserved inhibitors iASPP iASPP binds to proline-rich domain of p53, thereby preventing binding of p53 Trang 27promotes its dissociation from iASPP Thereby, Pin1 ... proteins, Pin1 affects their stability by interfering with this process 1.1.7.2 The role of Pin1 in protein stability A series of proteins can be stabilized by Pin1; these proteins include cyclin

Ngày tải lên: 12/09/2015, 09:55

... 24 2.1 Origin, breeding and rearing of fish 24 2.2 Observation of vas:egfp expression 24 2.3 Hematoxylin and Eosin staining 25 2.4 Immunohistochemistry 25 2.5 In situ hybridization ... machinery of sex determination and that selection pressure had led to the addition of new upstream regulators independently in different taxa (Wilkins, 1995; Zarkower, 2001) In the following introduction ... candidate gene inhibiting the expression of aromatase in zebrafish In mammals, AMH has also been confirmed to be able to inhibit expression of aromatase, in addition to its function in inducing Müllerian

Ngày tải lên: 14/09/2015, 09:39

... The list of BCH-containing proteins can be found in Figure 1.1 Trang 18Figure 1.1: Domain architecture and classifications of BCH-domaincontaining proteins Proteins containing the BCH domains can ... classifications of BCH-domaincontaining proteins. 6 1 2 The four domains of mesodermal C&E movements inthe zebrafish gastrula and their characteristic underlying 1 3 Description of morpholinos used ... tracking of cells or proteins, or for the monitoring of proteinactivity in the zebrafish embryo In addition, the zebrafish is permeable tosmall molecules in its aqueous environment, thus making

Ngày tải lên: 05/10/2015, 19:05

... activate intronless genes expression and inhibit expression of intron-containing genes (Ruvolo et al., 1998) In contrast to the majority of cellular genes, many EBV genes expressed during lytic ... profiling results of different EBV cell clines……… 54-61 Fig 7 DNA profiling results of one NPC biopsy……… ……….76 Fig 8 DNA profiling results of one non-NPC tissue biopsy……….77 Fig 9 RNA Profiling ... methylation of some promoters of cellular genes, including the promoters of tumour suppressor genes, and results in down-regulation of the expression of these tumour suppressor genes 1.2.5.1.2 Oncogene

Ngày tải lên: 05/10/2015, 22:32

... C A single colony was inoculated into 3 ml of LB broth and incubated overnight at 37° C The next day, 1 ml of such culture was inoculated into 100 ml of LB broth in a 250 ml flask and incubated ... Quantitative-PCR analysis of expression in ES cell culture 52 3.3 Z YGOTIC EXPRESSION EXAMINATION 54 3.3.1 Gene Cloning in O celebensis 54 3.3.2 Sequence analysis 55 3.3.3 RT-PCR analysis using species-specific ... homologs/orthologs remain to be identified and characterized in lower vertebrates 1.2.3.1 Klf4 Klf4, which is a zinc finger transcription factors, has a defining role in maintaining self-renewal in ES cells

Ngày tải lên: 09/10/2015, 10:49

... 0.6 0.4 0.2 2 .5 1 .5 acgh 0 .5 5' chr20 47 950 000 3' 47 955 000 GSDMB 25 20 15 coverage 10 acgh chr20 380 650 00 3' coverage 5' BCAS4 40 30 20 10 acgh 380 750 00 38070000 chr20 5' 49430000 49 450 000 49470000 ... multiple lines of evidence suggesting the potential functional importance of the fusion genes, including the involvement of known oncogenic partner genes, exclusive expression of the partner genes ... expression of the exons of the 3’ partner genes taking part in the fusions Exonic expression of CEP 250 in ZMYND8-CEP 250 (upper), ENSG00000236127 in CSE1LENSG00000236127 (second from top), GSDMB in TATDN1-GSDMB...

Ngày tải lên: 09/08/2014, 22:23

... find that the voting method again improves the results, returning very similar numbers of correct genes as a 5v5 regularized t-test (Figure 5) The voting method returns 41% more of the top 50 ... 2004, 5: R70 GEO/R.t-test vote 2v2 Regularized t-test 2v2 GEO 2v2 t-test 25 20 15 10 50 100 150 200 Number of genes 250 300 15 10 250 300 Figure dystrophy dataset A comparison of each method in 1v1 ... value of the higher rank A final score was obtained by combining the results from each method in this way, and the genes were re-ranked on the basis of this score By using the 75% / 25% ratio, genes...

Ngày tải lên: 14/08/2014, 14:21

... 1401-1 450 1 451 - 150 0 1401-1 450 1 451 - 150 0 1401-1 450 1 451 - 150 0 951 -1000 901- 950 851 -900 801- 850 751 -800 701- 750 651 -700 601- 650 55 1-600 50 1 -55 0 451 -50 0 401- 450 351 -400 301- 350 251 -300 201- 250 151 -200 ... 801- 850 751 -800 701- 750 651 -700 601- 650 55 1-600 50 1 -55 0 451 -50 0 401- 450 351 -400 301- 350 251 -300 201- 250 151 -200 101- 150 che-2::PABP/acr -5: :PABP ranks 1001-1 050 951 -1000 901- 950 851 -900 801- 850 751 -800 ... 751 -800 701- 750 651 -700 601- 650 55 1-600 50 1 -55 0 1 -50 51 -100 Fraction Fraction Volume 6, Issue 2, Article R17 451 -50 0 401- 450 351 -400 301- 350 251 -300 201- 250 151 -200 101- 150 51 -100 1 -50 Fraction...

Ngày tải lên: 14/08/2014, 14:21

... Gli2 In addition, both N-terminal repressor domain and C-terminal activator domain containing VP16-like 153 Discussion TAF binding domain and CBP binding site are found in Gli2b, indicating a ... role of gli2/gli2b in development of the zebrafish brain, especially the hindbrain Our results indicated that Gli2b plays a more important role in regulating Hh signaling in zebrafish hindbrain ... 0. 15 100 12(12%) 28(28%) 54 (54 %) 6(6%) 0.3 100 15( 15% ) 13(13%) 49(49%) 23(23%) 0.6 50 7(14%) 5( 10%) 17(34%) 21(42%) 100 35( 35% ) - 11(11%) 54 (54 %) gli2bMMA 50 3(6%) 45( 94%) 2(4%) - gli2bSpl MO Injected...

Ngày tải lên: 15/09/2015, 17:10

... alignment of mouse Gli2 proteins indicated that the sequence similarity is high in the N-terminal, including the five zinc-finger domains and six putative Protein Kinase A binding domains However, ... expression in the hindbrain acquires a characteristic pattern at 32 hpf in the dorsal hindbrain (Fig.3-9A); it increased in the bilateral domains in the lateral hindbrain at the level of rhombomere ... gli2b is not affected in both medial and lateral domains of the hindbrain Thus, FGF signaling seems not involved in regulation of gli2b expression in the midbrain and hindbrain 112 Results A B C...

Ngày tải lên: 15/09/2015, 17:10