5 isolation of whole casein in the dairy industry

... in the hydrostatic pressure However, in the presence of dense gas, the melting point of the solid decreases as the pressure increases owing to the increasing solubility of gas in the solid The ... peptides in the dairy industry 12.8 Treatment of effluents and technical fluids in the dairy industry 12.9 Conclusions and future trends in the dairy industry 12.10 The egg ... branches; one going from the critical point of the component with the higher critical temperature, initially in the direction of the critical point of the other component and then rising sharply...

Ngày tải lên: 20/09/2015, 01:57

... are distinct proteins rather than the nonallelic isoforms of a single protein in pseudotetraploid X laevis Another cDNA encodes a 401-residue protein containing two N-terminal BIR domains and ... containing two BIR domains could inhibit apoptosis (Fig 6) However, the presence of the residue 219–269-containing PEST1 blocked its apoptosis-inhibiting function, and the further addition of the ... Thus, in both xEIAP ⁄ XLX and xXIAP, the C-terminal region containing the RING finger domain is required for the initial phase of proteolysis within h and also for the final degradation of caspase-generated...

Ngày tải lên: 30/03/2014, 16:20

... other common anthocyanin containing fruits and vegetables, the concentration of anthocyanins in purple-fleshed ISPs are in the same range as some of the highest anthocyanin producing crops like blueberries, ... the change in starch content as a percent of dry matter and was examined in this study to determine the amount of starch converted during hydrolysis In this case, the main effects of solvent ... 78.3% of the dry matter, leaving a residual starch content of at least 11.4% of the dry matter The highest change was observed in the hydrolysis of treatments extracted with acidified ethanol using...

Ngày tải lên: 05/05/2014, 08:39

... understanding of the molecular mechanism of WSSV infection and assembly, or be helpful for the control of virus infection in the future Conclusion The localization of the two proteins, VP51 and ... blotting analysis indicated that the two proteins were exclusively detected in nucleocapsid fraction (Fig 1b) To eliminate the possible interference of the remaining viral envelope proteins, ... any remaining bound proteins The low-pH treated sample was suspended in 0.2 ml of TM buffer Expression and purification of proteins VP51 and VP76 are the products of ORF wsv308 and wsv220 of WSSV...

Ngày tải lên: 20/06/2014, 01:20

... contain a distinct yolk nucleus (Balbiani's vitelline body) Towards the end of the vitellogenic period, or by the beginning of the final maturation, the germinal vesicle (nucleus), which in the ... re-organized through multiple insertions and deletions of intervening sequences during the evolution of the various lineages Characterization of the Vtg region in the genome of the rainbow trout, e.g., ... purposes In the present context, the term "zona radiata proteins" (Zr-proteins) will be used to identify the constituent proteins of the inner layer of the envelope that surrounds the oocyte of the...

Ngày tải lên: 13/08/2014, 13:20

... screening Half of the colony was streaked on SD/-Trp agar plate and the other half inoculated into 20µl of PCR mix PCR products were sequenced to determine the identity of the interacting proteins 115 ... contains a mutated NubG domain The fusion plasmids are then cloned into the yeast cell and expressed on the cell membrane If the prey protein interacts with the bait protein, this would bind the ... B virus and involved in the transcytosis of the virus [17] Further functional analysis of these HBsAginteracting cellular membrane proteins should shed new insights on their role in HBV morphogenesis...

Ngày tải lên: 02/11/2012, 11:08

... of the UV-absorbing peak indicated it was composed mainly of nucleic acid In the profile of the NaOH-extracted polymer (B), the elution volume (80 ml) of saccharide corresponded to that of the ... included the band corresponding to a molecular weight of 103,000 in the steaming-extracted polymer (1), the band corresponding to 38,000 in the NaOH-extracted polymer (2), the band corresponding to ... Ag-protein stain, the components varied greatly by polymer Several bands demonstrated the same mobility in both stains, suggesting that these bands were complexes of protein and saccharide These included...

Ngày tải lên: 05/09/2013, 09:08

... capable of predicting the specificity of RNA-binding proteins across entire families (such as the RRM superfamily), or of redesigning the specificity of these proteins, would be of equal importance in ... test Starting with each complex in the training set containing one or more RRM or KH domains, we extracted the protein coordinates and the four nucleotides bound at the center of the domain (for ... line represents the rank of cognate recognition sequences using the contactcounting score; the blue line represents the rank of these sequences using the distance-dependent potential The points...

Ngày tải lên: 18/02/2014, 16:20

... assess the dynamics and kinetic properties of proteins in living cells [1219] FCS detects the uctuations of uorescent intensity derived from the movement of a single uorescent molecule in a very tiny ... diagram of the constructs microinjected into the medaka eggs and transfected into HeLa cells The olvas, nanos and tudor coding sequences were joined to the C-terminal region of the GFP or RFP coding ... 3A) During the measurement of the PGC, the 10 Time (s) 15 20 Fig FCS and FRAP analyses of OlvasGFP in the PGC FCS was used to measure the movement of OlvasGFP into the cytosol out of the nuage...

Ngày tải lên: 18/02/2014, 16:20

... accurate definition of the C-terminal domain boundary Experimental Study of the interactions of the Anp32a LRR module determination of the dynamic features of the domain in solution, together with a ... with the aim of mapping the surface of interaction between the two proteins This method, which relies on the effect that binding of a molecule has on the electron distribution of another, causing ... by an induced fit mechanism but by semirigid docking of the partners onto the surface of the Anp32a LRR domain Interactions with the INHAT complex, mapped to the flexible C-terminus, may induce...

Ngày tải lên: 18/02/2014, 17:20

... based on the conserved amino acid sequences of the catalytic domain of members of the GH18 family resulted in the amplification of an expected 147 bp sequence Cloning and sequencing of this fragment ... composed of the sole Glyco_18 domain, the C-terminal tail of C gigas CLPs may not noticeably contribute to the structure and the function of these proteins Interestingly, Cg-Clp1 and Cg-Clp2 C-terminal ... from the oyster Crassostrea gigas [5] Interestingly, this protein, named C gigas chitinase-like protein (CgClp1) was found to be involved in the control of growth and remodelling processes in a...

Ngày tải lên: 19/02/2014, 00:20

... out in the linear range determined using serial dilutions of samples (Fig 2) Linearity consisting of continuous signal increase and of reproducible glycoprotein patterns was determined in the ... The signal intensity of each of the three protein bands was quantified by densitometry The mean values of the calculated signal intensities were analyzed for each of the N- or C-terminal binding ... when using C-terminal binding antibodies Our data show that the high signal intensity corresponding to the size of the nonglycosylated full-length protein indicated antibody binding at the structured...

Ngày tải lên: 19/02/2014, 02:20

... downstream of the initiator ATG in exon (Ex1) of the 67 exon-containing htt gene [5] Pathogenesis in HD correlates with the cleavage of mutated htt and the release of an N-terminal fragment bearing the ... analyzed The arrow indicates the position of the more intensively oxidized polypeptide in the assay The bracket underlines the domain (molecular mass range 10–40 kDa) of the oxyblots that contains the ... analyze the EGFP-containing inclusions Figure 1F shows that the expression of Hsp70 together with Hdj-1 (Hsp40) did decrease the average size of the EGFP-containing inclusion bodies (average size of...

Ngày tải lên: 19/02/2014, 06:20

... on the result of selection If the cutting sites are in the flexible loop of the native structure, they could prevent the selection of stable proteins By examining the structures of the proteins ... protein in the absence of any cofactors In this work, the authors used the method similar to that of Finucane et al [11] except that the protein A B-domain instead of His-tag was used to select the ... proteolysis of the target protein also removes the N-terminal domains and prevents the infection of phage in E coli (B) A library of target proteins with a tag can be fused to the g3p protein on the...

Ngày tải lên: 19/02/2014, 12:20

... immunoblotting data, it was of interest to re-examine the fate of calsequestrin by studying the entire complement of key Ca2+-binding elements in dystrophindeficient skeletal muscle fibres Because the ... temperature maintained at 90 °C for to aid the staining of protein spots The tray was then placed on a laboratory shaker for a further 10 at room temperature Destaining was achieved by placing gels in a ... combination of the pI value and molecular mass of individual 2D spots can be useful in the initial identification of proteins However, owing to the abnormal electrophoretic mobility of certain...

Ngày tải lên: 19/02/2014, 16:20

... Massachusetts, USA ACL Jing Jiang and ChengXiang Zhai 2007 Instance weighting for domain adaptation in NLP In Proceedings of the 45th Annual Meeting of the Association of Computational Linguistics, pages ... study the effect of different parameter settings There are several parameters in the framework: the number of generated seeds r, the number of new candidates k2 and the number of selections k in ... Oregon ACL Minqing Hu and Bing Liu 2004 Mining and summarizing customer reviews In Proceedings of the tenth ACM SIGKDD international conference on Knowledge discovery and data mining, pages 168–177,...

Ngày tải lên: 19/02/2014, 19:20

... present in the dataset The scopes of cues that are missing from the baseline system were not included in the results As the baseline system was not penalized for missing cue phrases, the results ... encompassed the cue word(s) and all words in the scope (shown in a box on Figure 1) The subtree rooted by this ancestor is the basis for the resulting lexico-syntactic rule The leaf nodes of the resulting ... 2010) The system, inspired by the NegEx algorithm (Chapman et al., 2001), uses a list of phrases split into subsets (preceding vs following their scope) to identify cues using string matching The...

Ngày tải lên: 20/02/2014, 04:20

... vector Vm of a morpheme m is a vector consisting of frequencies of all possible unigrams 126 The morpheme feature M F (mi ) is a pair of the surface string and the part -of- speech of mi The similar ... and Hirai, 2004) formalized the task of extracting NEs as a chunking problem of a sequence of characters instead of a sequence of morphemes In this paper, we keep the naive formalization, because ... from the label B and the label I for eight NE types, in order to distinguish them When the task of extracting Japanese NEs from a sentence is formalized as a chunking problem of a sequence of morphemes,...

Ngày tải lên: 20/02/2014, 09:20

... that the former is likely to be in the Cterminal half and the latter in the N-terminal half of the protein [29,48±51] Sequence-based structure prediction methods suggest that the caseins are of the ... (rather than b sheet from the appearance of the amide I ROA, see below) [18] The negative intensity in a similar region of the ROA spectrum of b -casein may have a similar origin The two caseins ... above In view of the close similarity of the ROA spectra of the casein, synuclein and tau proteins shown in this paper, we have reproduced in Fig from an earlier study [22], the ROA spectrum of...

Ngày tải lên: 21/02/2014, 15:20