... colonies intact Then, the cells colonies, which contained ovarian cells such as theca stem cells and granulosa cells (Honda et al., 2007; Kossowska-Tomaszczuk et al., 2009) The filtered cells were ... most of the cells, including the ovarian cell colony-derived cells The dispersed cells were then of theca stem cells, followed by culture on dishes coated with gelatin (1:1 dilution) Under these ... resulted in the transformation of induced embryonic stem cells (ESCs) into PGC-like cells The PGC-like cells then went on to contribute to the pool of OLCs in the reconstituted ovaries These studies
Ngày tải lên: 16/12/2017, 04:38
... normal cells and tissues In other work, we have demonstrated that nor-mal mouse embryo fibroblasts and human primary fibro-blasts and epithelial cells and microvascular endothelial cells and primary ... of Ras itself (the pancreatic cancer cells are K-Ras mutant, and the melanoma cells are N-Ras mutant) MCF7 and the primary prostate and breast cancer stem cells, for example, had normal Ras (See ... pro-portion of cells expressing CD44, a candidate surface marker of breast cancer stem cells, and/or a smaller pro-portion of cells expressing the non-stem cell marker CD24, compared with adherent cells
Ngày tải lên: 05/11/2020, 01:11
class 72 welcome to our teachers welcome our teachers where are the students what are they doing when do they often do these games thursday october 29th2009 unit 5 work and play period 3010
... Trang 16What are they doing? They are playing catch Trang 17 They are reading1 2 3 4 5 6 7 What are they doing? Trang 18What are they doing?They are playing marbles Trang 19What are they doing?1 ... past five, the bell rings and all the students go in to the yard It’s time for recess They are all happy and excited They meet their friends and have some fun Many are talking about the last ... 4 5 6 7 They are eating, drinking and chatting Trang 20What are the boys doing? They are talking Trang 21What are the girls doing? 1 2 3 4 5 6 7 They are skipping rope Trang 22b.Ask and answer
Ngày tải lên: 19/04/2021, 19:04
where are the easter eggs
... 2In the nestOn the nest Beside the nest Trang 3Among the basketIn front of the basket Behind the basket Trang 4Beside the bunnyAmong the bunny Over the bunny Trang 5In front of the bunnyUnder the ... of the tulips Behind the tulips Trang 13Behind the bunnyAround the bunny In front of the bunny Trang 14Over the signUnder the sign Between the sign Trang 15On the stickAt the stick Right of the ... 16Behind the trainIn the train Over the train Trang 17Behind the chicksIn front of the chicks Betwwen the chicks Trang 18On the signIn front of the sign Between the sigh Trang 19Left and rightOver and
Ngày tải lên: 04/12/2016, 16:47
Comparison of oxidative stress effects on senescence patterning of human adult and perinatal tissue derived stem cells in short and long term cultures federica facchin1,2*, eva bianconi1,2*,
... cell-based therapy since their isolation is minimally invasive and because they are abundant and rapid in proliferation On the other hand, in the human body, these cells, as well as all other adult ... multi-lineage differentiation and the equilibrium between these two events determines the stem cell fate and their roles in the human body [1] They can be isolated and expanded in vitro from virtually ... activity, the capability of these cells to undergo passages, the morphological changes and the gene expression of tumor protein p53 (TP53, alias p53) and cyclin The comparison between the hASC and
Ngày tải lên: 15/01/2020, 02:05
Identification and characterization of cancer stem cells in human head and neck squamous cell carcinoma
... upper surface of the membrane with a cotton swab, and cells on the bottom surface of the membrane were fixed and stained with H&E Cells in five random fields per well were counted The experiments ... CD24+/CD44+ and CD24-/CD44+ cells The CD24+/CD44+ cells show significantly higher number of colonies P values for invasion and clonogenic assays are shown in the figure. Trang 7cells formed tumors and ... initiation, growth, and invasion of cancer are driven by a small population of cancer stem cells (CSC) Previous studies have identified CD44+ cells as cancer stem cells in head and neck squamous
Ngày tải lên: 05/11/2020, 01:35
biomaterials approach to expand and direct differentiation of stem cells
... by other organs such as muscles, the pulmonary system, the kidney, and endocrine glands Therefore, selection of biomaterials and the design of a tissue construct for repairing these organ systems ... cell signaling between stem cells and also between stem cells and their natural cellular niches Members of the Wnt protein family promote self-renewal of HSCs57 and MSCs58 and induce neural differentiation ... pluripotent stem cells for cell therapy and regenerative medicine EG cells are derived by the adaptation of primordial germ cells to survive and self-renew in culture.17,162 Despite the lower ethical
Ngày tải lên: 01/11/2022, 09:01
identification and characterization of cancer stem cells in human head and neck squamous cell carcinoma
... upper surface of the membrane with a cotton swab, and cells on the bottom surface of the membrane were fixed and stained with H&E Cells in five random fields per well were counted The experiments ... CD24+/CD44+ and CD24-/CD44+ cells The CD24+/CD44+ cells show significantly higher number of colonies P values for invasion and clonogenic assays are shown in the figure. Trang 7cells formed tumors and ... initiation, growth, and invasion of cancer are driven by a small population of cancer stem cells (CSC) Previous studies have identified CD44+ cells as cancer stem cells in head and neck squamous
Ngày tải lên: 02/11/2022, 11:41
What is cross border e commerce, the current market size (globally and by major market), and what are the consumer behaviors and drivers for
... CBEC Then there are the major risks that come with CBEC which can affect the purchase attitude These are risks that can harm the consumer if precautions are not taken The 6 major risks of this are ... customs and eventually be placed into the care of the buyer The more the customer does this form of shopping the more they begin to understand the complex system that works to provide him with ... E-Commerce, the current market size (globally and by major market), and what are the consumer behaviors and drivers for this channel, and which areas provide the greatest potential in the next
Ngày tải lên: 18/01/2025, 19:50
Mesenchymal stem cells are efficiently transduced with adenoviruses bearing type 35-derived fibers and the transduced cells with the IL-28A gene produces cytotoxicity to lung carcinoma
... system, to cells is mediated primarily by the binding of Ad fibers, which include the shaft and the knob regions, to the cel-lular receptor, the coxsackie adenovirus receptor (CAR) molecules, and ... Trang 5the expression on MSCs together with immortalized fibro-blasts, OUMS-24 and HFF cells, and esophageal carcin-oma TE-11 cells as a reference of normal cells and as a positive control for the ... AccessMesenchymal stem cells are efficiently transduced with adenoviruses bearing type 35-derived fibers and the transduced cells with the IL-28A gene produces cytotoxicity to lung carcinoma cells co-cultured
Ngày tải lên: 14/10/2020, 16:11
Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"
... system [10] When, the anesthetized nude mice were placed on the platform, the background image was taken under the lights of an illuminator Trang 3Then, the illuminator was turned off, and the ... circulation are adherent to the vascular endothelial cells, cross the endothelial cells, migrate and finally reached at ischemic sites In-flammatory may be observed in the local ischemic tissues, and ... head The normal femoral head was smooth and round, and the articular cartilage was transparent and glossy (A) After freezing, the femoral head was pale in the absence of normal glossiness and
Ngày tải lên: 25/10/2012, 11:18
Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
... factor (LIF) These cells express the Rex-1 and b-actin genes but not the embry-onic and adult globin genes (Fig 2) Upon differenti-ation the embryonic- and adult-specific b-globin genes are sequentially ... experimindepend-ents The results show that RNA Pol II, TPB, and Me2K4H3 are present at the core regions of the LCR (HS2 and HS3) but not at the ec- and bmajor-globin genes in undifferentiated ES cells (day ... At this time point, the LCR elements and the genes are associated with dimethyl H3K4 and acetylated H4 None of these marks are present in a region flanking HS2 and HS3 or in the murine necdin gene
Ngày tải lên: 07/03/2014, 12:20
Stem CELLS and the FUTURE OF REGENERATIVE MEDICINE pdf
... leading stem cells for stem cells for stem cells that to birth of a child research and research and are genetically recipient for research and therapy Starting material Isolated stem cells Cells ... called the trophoblast,and the cluster of cells inside the sphere is called the inner cell mass Atthis stage, there are about 70 trophoblast cells and about 30 cells in theinner cell mass The cells ... site,www.nationalacademies.org/stemcells.) The participants discussed the science of stem cells and avariety of ethical and other arguments relevant to public policy as it applies to stem cells The committee considered the
Ngày tải lên: 15/03/2014, 09:20
Báo cáo khoa học: A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells doc
... genes a Of the genes analyzed, the GO biological process is known for 739 up-regulated genes and 215 down-regulated genes Others are unknown.bOf the genes in the mother population, the GO biological ... 12 441 Others are unknown.cThe significance of the appearance of the GO term (biological process) in the up-regulated and down-regulated genes was calculated as a P value by the soft-ware GO Term ... networks are precisely regu-lated in the hepatic differentiation system, and that the AT-MSCs differentiate into mature hepatocytes Mesencyhmal-to-epithelial transition is the reverse of the epithelial–mesenchymal
Ngày tải lên: 30/03/2014, 04:20
Tế bào gốc và ứng dụng trong y sinh học (Stem cells and the application in biomedicine) potx
... Trang 1 Tế bào gốc và ứng dụng trong y sinh học (Stem cells and the application in biomedicine) Nguyễn Thị Thu Hà* Bệnh viện Trung ương Quân đội 108 ... bào thai người (Human Embryonic Stem cell) Năm 1998 nhóm nghiên cứu của James Thomson đã phân lập và duy trì được một loại tế bào gốc bào thai (ES: embryonic stem cell) từ khối tế bào bên trong ... phát triển trong môi trường nuôi cấy tổ chức sau khi chúng được tách ra từ phôi hoặc bào thai Tuỳ theo điều kiện của môi trường nuôi cấy mà những tế bào gốc bào thai có thể tạo nên những cụm tế
Ngày tải lên: 02/04/2014, 22:20
báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... miR-200c, miR-302b, and miR-367 between hES cells and EB, and between hES cells and adult cells was significant (P < 0.05) The difference in miR-302c expression between hES cells and adult cells was also ... to understand the molecular mechanisms responsible for the maintenance of the undifferentiated status and the differentiation process of human embryonic stem cells MicroRNAs (miRNAs) are small ... mouse embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that are directly targeted
Ngày tải lên: 18/06/2014, 15:20
Báo cáo y học: "The need for prognosticators in rheumatoid arthritis. Biological and clinical markers: where are we now" potx
... neoepitope Within the COL2 network, other components of the cartilage matrix are interspersed These components are degraded in the course of cartilage damage Among these are aggrecan, the breakdown ... the periphery often does not reflect the events occurring in the joint, but these by themselves are also not sufficiently predictive With the exception of macrophage infiltrates, therefore, the ... may therefore allow for optimized understanding and prediction of the fate of the individual RA patient There is recognition that clinical assessment (measuring and noting the change in index and
Ngày tải lên: 09/08/2014, 10:23
Báo cáo y học: "Human infrapatellar fat pad-derived stem cells express the pericyte marker 3G5 and show enhanced chondrogenesis after expansion in fibroblast growth factor-2" potx
... passages, including the clonally expanded cells The cells showed cell surface epitope expression similar to adult stem cells They stained strongly for CD13, CD29, CD44, CD90, and CD105 and were negative ... mesenchymal stem cells) and for CD29 (β1 integrin) (Figure 3) The cells stained poorly for LNGFR and STRO1, which are markers on freshly isolated bone marrow-derived stem cells, and stained sparsely ... the tunica intima consisting of the endothelial layer and the basement membrane, and αSMA (e, f) staining predominantly the tunica media consisting of the muscular layer of the arteriole are
Ngày tải lên: 09/08/2014, 10:23
Báo cáo y học: "Adipose-derived mesenchymal stem cells from the sand rat: transforming growth factor beta and 3D co-culture with human disc cells stimulate proteoglycan and collagen type I rich extracellular matrix" ppsx
... CD34 [23] These sand rat-derived AD-MSC are positive for the markers CD29 and CD105 and negative for the haemat-opoietic markers CD45 and CD34 The readily available anti-human markers CD90 and CD44 ... collagen of the nucleus pulposus It should be noted that the ECM com-ponents identified here are not exclusive to the annulus fibro-sis, and are also present in the ECM of the nucleus pulposus and cartilage ... interests The authors declare that they have no competing interests Authors' contributions HEG and ENH conceived the study and participated in its design and co-ordination HT and HEG wrote the manuscript
Ngày tải lên: 09/08/2014, 10:23
Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... separated by the proximity of the cells to the joint space and the bone respectively, alignment of the chondrocytes along the arcades of Benninghoff [29] and by the appearance of hypertrophic cells ... points in the BMP-2-modified aggregates compared with the GFP controls and BMP-4 transduced cultures, whereas signif-icantly higher values in the BMP-4 modified cultures compared with the GFP controls ... isolated and purified according to standard protocols [21] The iso-lated λ gt10 DNA was then digested with EcoRI to release the 1.7 kB sized BMP-4 cDNA insert, which was then cloned into the EcoRI
Ngày tải lên: 09/08/2014, 14:22