3 somatic cell sources for human induced pluripotent stem cells

... pluripotent stem cells and embryonic stem cells are distinguished by gene expression signatures Cell Stem Cell 2009, 5:111-123 93 Chin MH, Pellegrini M, Plath K, Lowry WE: Molecular analyses of human ... Pellegrini M, Plath K, Lowry WE: Molecular analyses of human induced pluripotent stem cells and embryonic stem cells Cell Stem Cell 2010, 7:263-269 94 Deng J, Shoemaker R, Xie B, Gore A, LeProust EM, ... pluripotent embryonic stem cells and differentiation Nat Rev Mol Cell Biol 2006, 7:540-546 51 Park SH, Kook MC, Kim EY, Park S, Lim JH: Ultrastructure of human embryonic stem cells and spontaneous...

Ngày tải lên: 11/08/2014, 12:21

... differentiated cells is undone The resultant cells, known colloquially as induced pluripotent stem cells (iPSCs), become akin to pluripotent embryonic stem cells (ESCs) and pluripotent cells of the ... utilised before a successfully reprogrammed cell is obtained 1.7.3 Cell explantation Cell explantation is a technique whereby immortalised cells are selected for from a culture of somatic cells (Figure ... pluripotency Cell Stem Cell, 2007, 1(1): 39-49 Figure Different methodologies to reprogram Somatic cells can be reprogrammed into pluripotent cells by four primary methods: nuclear transfer, cell fusion,...

Ngày tải lên: 09/09/2015, 18:56

... of human SOD1G37R astrocytes on motor neurons derived from human embryonic stem cells Cell Stem Cell 2008, 3:649-657 52 Di Giorgio FP, Boulting GL, Bobrowicz S, Eggan KC: Human embryonic stem cell- derived ... basal forebrain cholinergic neurons from human embryonic stem cells Stem Cells 2011, 29:802-811 45 Marchetto MCN, Carromeu C, Acab A, Yu D, Yeo GW, Mu Y, Chen G, Gage FH, Muotri AR: A model for ... Gage FH, Goldstein LS, Carson CT: Cell- surface marker signatures for the isolation of neural stem cells, glia and neurons derived from human pluripotent stem cells PLoS One 2011, 6:e17540 48 Funato...

Ngày tải lên: 11/08/2014, 12:21

... pluripotent stem cell lines derived from human somatic cells Science 2007, 318:1917-1920 Okita K, Nakagawa M, Hyenjong H, Ichisaka T, Yamanaka S: Generation of mouse induced pluripotent stem cells ... live cells EMBO J 2007, 26:4252-4262 Shi Y, Do JT, Desponts C, Hahm HS, Schöler HR, Ding S: A com bined chemical and genetic approach for the generation of induced pluripotent stem cells Cell Stem ... are therefore needed for this method to be more widely applied The first iPSCs derived from murine somatic cells were reported three years ago, followed by similar studies in human cells a year...

Ngày tải lên: 14/08/2014, 21:20

... Embryonic Fibroblast (MEF) cells as feeder cells for hESCs 37 4.2 Culture of Human Embryonic Stem Cells (hESCs) 38 4.2.1 Culture of Human Embryonic Stem Cells on MEF feeders 38 4.2.2 ... induction of Pluripotent Stem Cells ……………… 44 4.8 Infection of fibroblast cells to form Induced Pluripotent Stem Cells …………………………………………………………………… 45 4.9 Culture of cells transitioning from ... induce the cells to pluripotent cells These pluripotent cells are studied and characterized The pluripotent stem cells exhibited morphology and genomic markers of pluripotency These cells were...

Ngày tải lên: 30/09/2015, 10:13

... PGE2-promoted cellular motility in human LoVo cells LoVo cells were pretreated with vehicle, SP600125 (JNK1/2 inhibitor, μM) or 17b-estradiol (10-8M) for h prior to PGE2 (10-6M) treatment for another ... and TIMPs in human LoVo colon cancer cells LoVo cells cultured in DMEM were treated with PGE2 (10-6M) for h, h,12 h and 24 h, and then were harvested and lysed Total protein of cell extracts ... expression of uPA and MMP-9 within 24 h in human LoVo colon cancer cells (Figure 4B) 17b-Estradiol Inhibits PGE2-Induced Cell Migration in Human LoVo Colon Cancer Cells In the present study, we examined...

Ngày tải lên: 10/08/2014, 10:20

... 2.2 Stem cells in tissue regeneration 2.2.1 Embryonic stem cells 2.2.2 Adult stem cells 2.2.3 Adipose derived stem cells Chapter Chapter v 2.2.4 Hepatic differentiation of adipose derived stem ... Injectable deliverey systems for stem cell therapy 20 2.4.1 Effect of mechanical cues on stem cells 21 2.4.2 Effect of biomolecular cues on stem cells 22 2.4.3 Hydrogels for stem cell therapy 23 2.4.4 ... various types of stem cells for such stem cell based therapies, few of which are discussed in the following sections Stem cells are broadly classified into embryonic and adult stem cells based on...

Ngày tải lên: 09/09/2015, 11:18

... and Y T Chang Diversity Oriented Fluorescence Library Approach for Stem Cell Probe Development 2011 Stem Cells and Cancer Stem Cells, Volume 3, 1007/978-94-007 34) R K Das, A Samanta, K Ghosh, ... staining and interaction with the stem cells and a better understanding of what is resulting in pluripotent stem cells to bind specifically to CDy9 as compared to the other cells This may eventually ... Sato, T Nakano, Embryonic Stem cell Internal Medicine 2001 Vol 40, No 4) JM Slack Stem Cells in Epithelial Tissues 2000 Science 287, 1431–1433 5) A I Caplan Mesenchymal stem cells J Orthop Res., 1991...

Ngày tải lên: 02/10/2015, 17:14

... expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells The exploration and realization of the incredible potential of these pluripotent stem cells for studying ... differentiated human pluripotent stem cells is demonstrated in the subsequent chapter Karyotype stability of human pluripotent stem cells is sensitive to growth conditions and to the manner in which cells ... demonstrated that ESC-like cells can be isolated from human embryos They described the isolation of ICM cells from human blastocysts and the culturing of these cells for two passages, while expressing...

Ngày tải lên: 22/03/2014, 09:20

... 000 cells were collected from each population (b) Cells from bright population were seeded onto mitomycin treated feeder cells for weeks before addition of 1uM of CDy9 and image acquisition Cells ... differentiated states of the cells (refer to Table 2) 21 primers were chosen that define the pluripotent states of the cells These genes are upregulated in ES cells and are involved in stem cell initiation, ... from a mixture of cells It is also important to note that CDy9 does not only stain a colony of stem cells and allow easy identification and isolation but it also allows single stem cells to be detected...

Ngày tải lên: 02/10/2015, 17:14

... adult stem cells are hematopoietic stem cells which are formed in the bone marrow These cells play a role by differentiating and replacing the blood cells in the body Other types of adult stem cells ... function (1 - 3) Typically, stem cells can be broadly classified into main groups, the embryonic stem cells and the somatic or adult stem cells The latter, adult stem cells are found in various ... for mouse pluripotent stem cells to enable live imaging of the cells with very minimal or no alteration to the cell s function or capabilities Introduction to stem cells Stem cells are a unique...

Ngày tải lên: 02/10/2015, 17:14

... ES cells and in human hematopoietic progenitor cells Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells ... the developmental progression of cell samples and in all cases except that of the Rex-1 gene, primer sets span introns Day cells are composed of ES cells and MEF cells grown in ES media containing ... progenitor cells or cells that have yet to express the genes at physiologically relevant levels [11] Chromatin structure modifications in uncommitted progenitor cells have also been observed for the...

Ngày tải lên: 07/03/2014, 12:20

... from human placenta Stem Cells 22, 1338–1345 Bieback K, Kern S, Kluter H & Eichler H (2004) Critical parameters for the isolation of mesenchymal stem cells from umbilical cord blood Stem Cells ... hepatic cells, the findings presented here suggest that MET might be a pivotal factor in determining stem cell transdifferentiation Discussion AT-MSCs may be good candidates as stem cells for cell ... differentiation from stem cells For microarray analysis, we confirmed the hepatic differentiation of AT-MSC into hepatocyte-like cells using the original protocol (Fig 1A) The differentiated cells (AT-MSC-Hepa)...

Ngày tải lên: 30/03/2014, 04:20

... not for citation purposes) Journal of Negative Results in BioMedicine 2009, 8:11 days At 80-90% confluence (about 10,000 cells/ cm2), stem cells were trypsinized with stem cell trypsin (CellSystems, ... properties, suppressing the activity of a broad range of immune cells, including N -cells, Bcells, natural killer cells and antigen presenting cells Transplantation of MSCs into the periinfarct area decreases ... medium, and the cells were seeded in 10 cm culture dishes After 24 hours, non adherent (hematopoietic) cells were removed by medium change Mesenchymal stem cells were expanded in stem cell culture...

Ngày tải lên: 11/08/2014, 07:21

... pluripotent stem cells Cell Stem Cell 2010, 7:249-257 Wilson KD, Venkatasubrahmanyam S, Jia F, Sun N, Butte AJ, Wu JC: MicroRNA profiling of human- induced pluripotent stem cells Stem Cells Dev ... ESC, embryonic stem cell; hESC, human embryonic stem cell; hiPSC, human induced pluripotent stem cell; H3K4me3, histone H3 lysine trimethylation; iPSC, induced pluripotent stem cell, lincRNA, ... in mouse induced pluripotent stem cells Nature 2010, 465:175-181 Newman AM, Cooper JB: Lab-specific gene expression signatures in pluripotent stem cells Cell Stem Cell 2010, 7:258-262 Polo JM,...

Ngày tải lên: 11/08/2014, 12:21

... cancer stem cell model where clonal evolution is occurring only in a subset of the cancer cells (the stem cells) we can see that for any size of tumour, because of the much smaller number of stem cells, ... representing a tumour of a given size The number of stem cells is calculated by assuming one stem cell per 57 000 non -stem cells [28] A greater proportion of stem cells would result in a less dramatic difference ... selection of stem cells and non -stem cells and thereby indirectly testing the entire stem cell paradigm Finally, it might be assumed that selection pressure acting on cancer cells would be in the form...

Ngày tải lên: 13/08/2014, 16:20

... signature Stem cells Stem cells (c) Committed basal Committed basal Stem Committed cells basal (d) Malignancy signature Tumour Birnie et al R83.3 Gleason included Benign Figure Distinctive stem cell ... SL performed the stem cell isolations from benign samples PB performed stem cell isolations from tumors CH performed culturing and isolation of stem cells, and NF-κB experiments JLL performed ... cancer cells PTL induces apoptosis in primitive cancer cells Percent viability of prostate cancer cells and cells from a patient with BPH treated with increasing concentrations of PTL Cells were...

Ngày tải lên: 14/08/2014, 08:21

... transfected into A2058 cells by selecting the cells with G418 Cells were washed with phosphate-buffered saline (NaCl/Pi) and grown in complete medium for 36 h before analyses of cell motility, immunoprecipitation ... essential role in cell motility [29] and proliferation [30] In order to test the effects of LPA on PAK1 activity in A2058 human melanoma cells, serum starved cells were stimulated by lM LPA for various ... neuroblastoma cells [34] Therefore, the possible involvement of Fig LPA stimulates PAK1 in A2058 cells The cells were serumstarved for 12 h and then stimulated with lM LPA for the indicated time points...

Ngày tải lên: 30/03/2014, 13:20

... Embryonic stem cells 20 1.2.1.1.2 Induced pluripotent stem cells 21 1.2.1.2 Adult stem cells 23 1.2.1.2.1 Mesenchymal stem cells 23 1.2.1.2.2 Neural stem cells 23 1.2.2 Clinical relevance of stem cell ... carriers for cancer therapy 13 1.1.2.1 Viral vectors 13 1.1.2.2 Nonviral vectors 16 1.2 Stem cells- unique candidates for therapeutic platform 1.2.1 What are stem cells 1.2.1.1 Pluripotent stem cells ... alternative large scale production of therapeutic stem cells must be identified Pluripotent stem cells including embryonic stem cells and induced pluripotent stem cells may hold the answer to this question...

Ngày tải lên: 09/09/2015, 10:08

... Introduction 1.1 Stem cells Stem cells have the remarkable potential to differentiate into specialized cells and thereby are key factors for the development of the whole organism All types of stem cells ... AAV system, each shown in cell culture on 16 transformed cells and in vivo with somatic cells, respectively However, until now this system has not been used on human somatic cells in a cell culture ... that human neural stem cells (lt-NES cells) , being precursors of glia und neurons were also suitable candidates for transduction 1.3.3 Alternative systems for genetic modifications of human cells...

Ngày tải lên: 26/11/2015, 09:54