3 bone regeneration and reformation

... reviewed here, and an ability to manipulate and/or isolate and cul-tivate large numbers of human osteoprogenitor cells (MSCs), some clinical therapies to achieve bone(and other tissue) regeneration ... bone repair, and skel-etal regeneration therapy J Cell Biochem 56(3), 283–294. 2 Caplan, A I and Pechak, D (1987) The cellular and molecular biology of bone formation, in Bone and Mineral Research, ... 117–183. 3 Caplan, A I and Bruder, S P (1997) Cell and molecular engineering of bone regeneration, in Textbook of Tissue Engineering (Lanza, R., Langer, R., and Chick, W., eds.), R.G Landes, Georgetown,...

Ngày tải lên: 11/08/2014, 05:20

... Publication Data Bone regeneration and repair : biology and clinical applications / edited by Jay R Lieberman and Gary E Friedlander. p ; cm. Includes bibliographical references and index. ISBN ... Trang 1Bone Regenerationand Repair Bone Regeneration Trang 2Contents iBone Regeneration and Repair This is trial version www.adultpdf.com Trang 3ii ... 1-59259-863-3 1 Bone regeneration 2 Regeneration (Biology) 3 Transplantation of organs, tissues, etc. [DNLM: 1 Bone Regeneration—physiology 2 Bone Regeneration—genetics 3 Bone and Bones—physiology....

Ngày tải lên: 11/08/2014, 05:20

... osteoblasts (128) replaced by types II and IX collagen –Type V and XI may regulate the and very little type I collagen (122) growth and orientation of type I and –Type II collagen mRNA is detectable ... II and V (124) or nonunion (124) –Mechanically unstable fractures are –Type IV (and types I and X) may aid characterized by initial production of in converting mesenchymal lineage types III and ... proliferatingosteoblasts In chondrogenesis and endochondral ossification, BMP-2 and -4 are found in proliferat-ing chondrocytes, weakly in mature and hypertrophic chondrocytes, and strongly in osteoblasts nearendochondral...

Ngày tải lên: 11/08/2014, 05:20

... or Ad.luciferase (panels E–H) and radiographed at the time of surgery (panels A and E) and after 5 wk (panels B and F), 7 wk (panels C and G) and 12 wk (panels D and H) Defects treated with Ad.BMP-2 ... Fernandez, D L., Ring, D., and Jupiter, J B (2001) Surgical management of delayed union and nonunion of distal radius fractures J Hand Surg 26(2), 201–209. 2 Rommens, P M., Coosemans, W., and ... 18From: Bone Regeneration and Repair: Biology and Clinical Applications Edited by: J R Lieberman and G E Friedlaender © Humana Press Inc., Totowa, NJ 10 Bone Morphogenetic Proteins and Other Growth...

Ngày tải lên: 11/08/2014, 05:20

... Bone Regeneration and Repair: Biology and Clinical Applications Edited by: J R Lieberman and G E Friedlaender © Humana Press Inc., Totowa, NJ 11 The Ilizarov Technique for Bone Regeneration and ... B., Jorgensen, P H., and Andreassen, T T (1990) Dose response of growth hormone on fracture healing in therat Acta Orthop Scand 61, 54–57. 170 Bak, B., Jorgensen, P H., and Andreassen, T T (1990) ... and Mohan, S (1997) Insulin-like growth factor binding proteins in serum and other bio-logical fluids: regulation and functions Endocr Res 18, 801–831. 160 Copeland, K C., Underwood, L E., and...

Ngày tải lên: 11/08/2014, 05:20

... 237–252. 14 Weiland, A J., Kleinert, H E., Kutz, J E., and Daniel, R K (1979) Free vascularized bone grafts in surgery of the upper extremity J Hand Surg 4(2), 129–144. 15 Sowa, D T and Weiland, A J ... osteonecrosis of thefemoral head, congenital tibial and forearm pseudarthrosis, congenital differences and pediatric trauma,and to facilitate spine and joint arthrodesis Although vascularized fibula ... Gilbert and Brock-man’s series and 26% of the patients in Weiland’s series required secondary surgical procedures toachieve ultimate union In addition, residual tibial malalignment and leg length...

Ngày tải lên: 11/08/2014, 05:20

... segmental bone defects Aebli and colleagues (Aebli et al., 2005) and Saito and colleagues (Saito et al., 2005) reported that BMPs improve bone regeneration in vivo, and BMP-2 has been found to ... growth factor, fibroblast growth factor and insulin growth factor have been shown to stimulate bone growth, collagen synthesis, and fracture repair both in vitro and in vivo (Jingushi et al., 1995; ... Trang 1in bone repair and regeneration Treatment of fractured bone with cells, growth factors and biomaterials are the three commonly used ways over the past...

Ngày tải lên: 14/09/2015, 08:41

... (Conwell and Huang, 2005; Schreier, 1994; Trang 3Tomlinson and Rolland, 1996) There have been two major approaches proposed: the viral mediated and non-viral mediated gene transfection (Ledley and ... cells and immune system cells (e.g., lymphocytes and tissue cells from spleen, thymus, and lymph nodes) For the circulatory system and bone marrow in which cells can occur in a liquid suspension and ... design, in the aspect of interactions between material and gene, material and cells as well as gene and cells In order to tackle the disadvantages and shortcomings with current drug delivery devices,...

Ngày tải lên: 14/09/2015, 08:41

... calculated and used to determine the secondary structure of the protein using procedures reported by Nahar and Riahi (Nahar and Riahi, 1996) Trang 11Tajmir-3.3.5 Cell culture, cell attachment and cytotoxicity ... microscope and compared after day 1, 2, 3, 4, 5, 6, 7 and 8, respectively Trang 123.3.6 Statistical analysis All the data were statistically analyzed to express the mean ± standard deviation (S.D.) and ... molecular integrity and biological activity of BMP-2, in vitro release sample was centrifuged and the supernatant was analyzed by Native-PAGE to determine the integrity and conformation of BMP-2...

Ngày tải lên: 14/09/2015, 08:41

... for bone regeneration, a new kind of scaffold is needed because nanoparticles and microparticles are not suitable in view of their fluidity, and hence can’t be localized themselves and give new ... US) and purchased from Lakeshore Biomaterials (Birmingham, England) Chitosan (medium molecular weight and 75-85% deacetylated), chitosanase from Streptomyces griseus (lyophilized powder) and ... analyzed to express the mean ± the standard deviation (S.D.) of the mean and p<0.05 was accepted to be significant 5.4 Results and discussion 5.4.1 pT7T3D-Pac purity and concentration In order to...

Ngày tải lên: 14/09/2015, 08:41

... from the sonication of organic and aqueous mixture Table 6.1 Trang 4summarizes the composition of the emulsion of the 3 groups (A, B and C) and 9 samples (A1-A3, B1-B3 and C1-C3) The detailed fabrication ... scaffold is denoted as control and white arrows identify bone defects The bone ends of the control samples were sharp and there was no significant bone regeneration after two and four weeks The delayed ... properties of groups A, B and C, and are consistent with the in vitro release profiles of BMP-2 plasmid from different scaffolds (Nie and Wang, 2007), As naked Trang 9DNA and DNA/chitosan nanoparticles...

Ngày tải lên: 14/09/2015, 08:41

... (Onishi and Machida, 1999) extracted from crustaceans and has been shown to be non-toxic in animals (Rao and Sharma, 1997) and humans (Aspden et al., 1997) Due to its good biocompatibility and cytotoxicity ... degrade gradually and give 3-dimensional structural guide in the formation and regeneration of tissue (Goldstein et al., 2001).Therefore, combining the concept of gene delivery and tissue engineering, ... release and adhering cells On the other hand, supercritical CO2 technique is a versatile foaming tool to produce micro-porous structures with particular pore sizes and shapes Here, the morphology and...

Ngày tải lên: 14/09/2015, 08:41

... The stable and uniform porous structures, tunable pore size and well-defined surface properties of these materials allows the incorporation and release of a diversity of proteins and DNAs in ... rate of DNA and subsequent expression of target protein is too low, especially in the initial stage (Figures 7.5a and 7.7b) Therefore, an initial and significant release of DNA is demanded in order ... K4 and K20 peptides and the surface physical properties of the foams were investigated using a series of state-of-the-art techniques, such as SEM and XPS BMP-2 plasmid was used as a model DNA and...

Ngày tải lên: 14/09/2015, 08:41

... NP-40, and 20% glycerol, supplemented with protease inhibitors (1 lgÆmL)1 leupeptin and pepstatin, lgÆmL)1 aprotinin, and mm phenylmethanesulfonyl fluoride) and phosphatase inhibitors (10 mm NaF and ... China (nos 2006CB504100 and 2006CB943703), the Nature and Sciences Foundation of China (no 30393130), the Hi-tech Research and Development Program of China (no 2006AA02A101) and Grant of Beijing ... dissociated into single cells and planted in four-well plates at a density of · 104 cells per well, and then cultured under different O2 concentrations (20% O2, 10% O2, and 3% O2) for days The number...

Ngày tải lên: 30/03/2014, 04:20

... contributions NHR and NAM designed experiments, interpreted data and conceptualized manuscript RH, JK, KWA, CAS, BM, ANP, MPM, LS, DTA, and TEI provided detailed ideas and discussions, and/ or writing ... ARDS and is associated with acute systemic inflammation and vascular damage Septic patients have elevated levels of injury associated signals and EPC mobilizers such as HMGB1 [63], SDF-1 [64], and ... into endothelial cells in vitro and in vivo based on expression of CD31, eNOS, and E-selectin [31] These studies were expanded into hindlimb ischemia in mouse and rabbit models in which increased...

Ngày tải lên: 18/06/2014, 15:20

... Inc., Cleveland, Ohio, USA) to be used in this study is isolated from a single bone marrow aspirate and cultured with heat inactivated fetal bovine serum (FBS) and growth factors EGF and PDGF Cells ... cells, including MAPCs and MSCs, have been shown to beneficially modulate the anti-donor immune response in organ transplantation and to promote tissue regeneration in vitro and in vivo [26-29,53] ... FCP, and HJS BF developed essential study documents EKG, PS, and PP supported the design of the study with their knowledge and experience MHD is the principal investigator of the study and the...

Ngày tải lên: 18/06/2014, 22:20

... both pulmonary and systemic inflammation, with induction of cytokines and chemokines and generation of oxidative species These injurious molecules create and exacerbate inflammation and oxidative ... angiographic results in phases and (the pilot phase and first randomized phase) For multivariate analysis, the treatment groups were categorized as follows: control, 0; CPC, 1; and BMC, The multivariate ... Abciximab and Device Investigation to Lower Late Angioplasty Complications] trial) and 4.1% (in the ADMIRAL [Abciximab before Direct Angioplasty and Stenting in Myocardial Infarction Regarding Acute and...

Ngày tải lên: 27/06/2014, 00:20

... BCNU and cisplatin, of which the former is primarily used for treating brain cancers and Hodgkin’s lymphoma and the latter is used to treat a wide range of cancers (including breast, lung and ... cells, and NSCs (Figure 3) The preferential vulnerability of both rat and human primary CNS progenitor cells to BCNU and cisplatin was apparent also at very low exposure levels Even the BCNUand ... respectively, on days 1, 3, and 5) show marked and prolonged increases in cell death in the lateral subventricular zone (SVZ), the corpus callosum (CC) and the dentate gyrus (DG) at 1, 10, and 42 days following...

Ngày tải lên: 06/08/2014, 18:21

... (CD44) and intercellular adhesion molecule-1 (CD54), growth factor and cytokine receptors, integrins and additional markers such as tetraspan (CD9) and Thy-1 (CD90) In contrast, CD34 and the ... dioxide, and the medium was changed every third day After weeks the samples were used for histological and immunohistological studies, and for RT-PCR gene expression analysis Histology and immunohistochemistry ... (45 s at 72°C) and denaturation (60 s at 94°C); and terminating with 15 at 72°C PCR products were separated on a 1.5% agarose gel and stained with ethidium bromide, visualized and digitalized...

Ngày tải lên: 09/08/2014, 01:23