2the skin dendritic cell network

... LPS-activated dendritic cells conditioned with 1000 ng/ml nicotine (Fig-ure 4C; 10665 ± 106.1 vs 8205 ± 275.8 cells for acti-vated dendritic cells and nicotine conditioned LPS-activated dendritic cells ... dendritic cells, human lung dendritic cells are more chal-lenging to characterize [4,27] The trachea and large con-ducting airways have a well-developed network of intraepithelial dendritic cells ... by which dendritic cells accumulate in COPD, complimentary studies were conducted using monocyte-derived human dendritic cells exposed to cigarette smoke extract (CSE), and dendritic cells extracted

Ngày tải lên: 12/08/2014, 11:21

... from 6.8 cells/μl (range, 2.1 to 12.2 cells/μl) to 11.1 cells/μl (range, 1.6 to 27.2 cells/μl) but sharply decreased to the nadir on day 15 p.i (mean, 3.8 cells/μl; range, 1.2 to 6.4 cells/μl) ... of mDC before infection in the eight Ch Rhs was 70.7 cells/μl (range, 13.7 to 260.1 cells/μl); this fell to 25.0 cells/μl (range, 4 to 44.5 cells/μl) on day 15 p.i with an acute decrease, which ... correlations between CD4+ T-cell counts and DC subset counts or cytokine production (A) CD4+ T-cell counts and mDC counts; (B) CD4+ T-cell counts and pDC counts; (C) CD4+ T-cell counts and IL-12 concentration;

Ngày tải lên: 13/08/2014, 01:20

... replication and cell-cell transmission in CD4+T cells and macrophages Tetherin is a type I IFN-inducible restriction factor that blocks HIV-1 release and modulates CD4+T cell-mediated cell-to-cell transmission ... tetherin in the cell-to-cell transmission of HIV-1 mediated by CD4+ T cells [48-50] Casartelli et al reported that tetherin impairs cell-to-cell transmission of HIV-1 in several cell lines and ... expressed on target cells promotes HIV-1 cell-to-cell transfer, while tetherin expressed on donor cells inhibits viral transmission [50] The discrepancy between these studies may be due to cell-type-dependent

Ngày tải lên: 13/08/2014, 01:20

... Kapsenberg ML: Microbial com-pounds selectively induce Th1 cell-promoting or Th2-cell promoting dendritic cells in vitro with diverse Th cell-polar-izing signals J Immunol 2002, 168:1704-1709. ... immunodeficiency virus fusion to dendritic cells declines as cells mature J Virol 2006, 80:1992-1999. 44 Vanham G, Davis D, Willems B, Penne L, Kestens L, Janssens W, van der GG: Dendritic cells, exposed to ... transmitted to TEM cells, and CXCR4-using (X4) HIV-1 was preferentially transmitted to TN cells Conclusion: The highly efficient R5 transfer to TEM cells suggests that mucosal T cells are an important

Ngày tải lên: 13/08/2014, 09:20

... effectiveness of the dendritic cell culture pro-tocol, we performed flow cytometry for the MDDC phe-notype After 8 days in culture, cells were stained for HLA-DR and CD83 Immature dendritic cells express ... peptide-loaded dendritic cell approach employing epitopes in the second coding exon of SIV Tat which spans reading frames for both Env and Rev We report here that autologous dendritic cells, loaded ... less-fit progeny viruses in vivo. Peptide loaded dendritic cells have been used in cancer immunotherapy and in viral vaccine efforts to induce a cellular response against specific epitopes [7,8]

Ngày tải lên: 13/08/2014, 09:21

... monocyte and dendritic cell subset frequencies in sepsis patients Circulating monocyte and dendritic cell subset frequencies in sepsis patients Frequencies of circulating monocyte and dendritic cell ... increased in sepsis [27-29] Dendritic cell-like monocytes CD14 dim CD16 positive Morphological and functional similarities to dendritic cells [42,43] Plasmacytoid dendritic cells lin1 - CD123 + HLA-DR ... Materials and methods, and is given as HLA-DR antibodies per cell (Ab/cell) (b) For plasmacytoid dendritic cells (PDCs) and myeloid dendritic cells (MDCs), the HLA-DR expression is given as the mean

Ngày tải lên: 13/08/2014, 18:22

... regarding the spe-cific functions of these cells Table 2 Expression of control genes in mouse cells Dendritic cells LymphocytesProbe set ID Gene Myeloid cells pDC CD8α DC CD11b DC NK CD8 T CD4 T ... genes were detected primarily in T cells and often to a lower extent in NK cells; the mouse Klrb1c (nk1.1) gene or the human KIR genes in NK cells; Cd19 in B cells; the mouse Siglech and Bst2 ... cells (2) MD/SCPK GEO GSE9810 X X Spleen CD4 T cells (2) AYR GEO GSM44979; GSM44982 X X X Spleen monocytes (2) BP GEO GSM224733; Spleen B1 cells (2) CB/DM GEO GSM66915; GSM66916 X Spleen NK cells

Ngày tải lên: 14/08/2014, 08:20

... Trang 1peripheral blood myeloid dendritic cells, monocyte-derived dendritic cells and the dendritic cell-like KG-1 cells reveals distinct characteristics Claire Horlock ... profiles human dendritic cells <p>Important proteomic and functional differences between peripheral blood myeloid dendritic cells, monocyte-derived dendritic cells (moDC) and KG-1 cells have ... http://genomebiology.com/2007/8/3/R30 Trang 2Dendritic cells (DCs) are highly specialized antigen present-ing cells that originate from bone marrow progenitor cells They represent a major cellular component of the

Ngày tải lên: 14/08/2014, 18:20

... tumor cells, concomitantly switches regulatory T cells off Dox also abrogates tumor and renders tumor cell debris immunogenic The DNA activated immune system kills the remaining neotransformed cells ... by dying cells induces the maturation of APCs J Immunol 2001, 167:2602-2607. 16 Boczkowski D, Nair SK, Snyder D, Gilboa E: Dendritic cells pulsed with RNA are potent antigen-presenting cells in ... Ishii KJ: Molecular and cellular mechanisms of DNA vaccines Hum Vaccin 2008, 4:453-456. 23 Martin DA, Elkon KB: Intracellular mammalian DNA stimulates myeloid dendritic cells to produce type I

Ngày tải lên: 14/08/2014, 19:22

... to cognate T cells by professional antigen-presenting cells (APC) Dendritic cells (DC) are professional antigen presenting cells (APC) with exquisite capability to activate naive T cells and hence ... Trang 10SUMMARY Dendritic cells (DC) are the most potent professional antigen-presenting cells (APC) with exquisite capacity to interact with T cells to initiate strong primary cellular immune ... the Ikaros DN-/- mice, differentiation of myeloid cells is normal but there is a severe defect in the development of lymphoid cells and dendritic cells [14] This suggests that myeloid progenitors

Ngày tải lên: 16/09/2015, 15:54

... in murine blood included CD8 cells (E), CD4 cells (F), CD3 cells (G), CD19 cells (H), CD45 cells (I), and CD56 cells (J) Abbreviations: HSCs, hematopoietic stem cells; FSC, forward scatter; ... from 1,788±665 cells/μL to 763±571 cells/μL, while in control mice, WBCs did not significantly change (1,659±571 cells/μL to 1,518±207 cells/μL) Busulfan also strongly decreased CD4 cells from 11.26%±2.45% ... activity and low T-cell activation Upon contact with an antigen, they become mature and can strongly activate the T-cells via cell–cell contact or by producing a pool of cytokines.7 These cells highly

Ngày tải lên: 16/12/2017, 01:16

... experimental and control groups T-cells (dCs:T-cells =1:10) T-cells (dCs:T-cells =1:20) T-cells (dCs:T-cells =1:40) Abbreviations: BCSC, breast cancer stem cell; dC, dendritic cell. 1 A G0 G1 G2 B C D ... (mononuclear cells) * denotes statistically significant difference. Abbreviations: BCSC, breast cancer stem cell; dC, dendritic cell; idC, immature dendritic cell; mdC, mature dendritic cell; TNF, ... of cells during differentiation process to dendritic cells Notes: Mononuclear cells were obtained from murine bone marrow (A) and induced to immature dendritic cells (B) or mature dendritic cells

Ngày tải lên: 16/12/2017, 03:01

... expressed on antigen-presenting cells (APC), e.g dendritic cells (DC), B cells and monocytes, but also found on some non-lymphoid cells such as epithelial and endothelial cells, fibroblasts, and some ... dendritic cells were also cyclically modulated, again without significant change between start and end of treatment CD8 and CD4 T cell populations, along with regulatory T cells, effector T cells, ... identify potential relevant biomarkers of clinical activity in Trang 3dendritic cells, CD8+ effector cells and regulatory T cellsin response to anti-CD40 agonist treatment in the con-text of chemotherapy

Ngày tải lên: 06/08/2020, 07:30

... of a specific T cell response Cytotoxic T cell assay and ELISpot assay revealed a tumor-specific T cell activity Conclusions: This study demonstrated that mEHT induces tumor cell apoptosis and ... marrow-derived dendritic cells Bone marrow-derived DCs (BM-DCs) were produced as described elsewhere [9] Briefly, BM-DCs were isolated from BALB/c mice by culturing red blood cell-depleted BM cells in ... binding of FITC-dextran to the cell surface was measured by incubating the cells at 4 °C [30] The percentage of positive cells was obtained using a FACSCalibur cytometer and Cell Quest Pro soft-ware

Ngày tải lên: 22/09/2020, 23:40

... production of dendritic cells from blood or bone marrow) Langerhans cells served as a prototype dendritic cell The basis of our current knowledge on the maturation of dendritic cells stems from ... subclasses of dendritic cells direct the development of distinct T helper cells in vivo J Exp Med 189, 587–592 Suss, G and Shortman, K (1996) A subclass of dendritic cells kills CD4 T cells via Fas/Fas-ligand- ... superantigens by thymic and splenic dendritic cells and B cells J Immunol 157, 2789–2794 Ardavin,C., Wu, L., Li, C.-L., and Shortman, K (1993) Thymic dendritic cells and T cells develop simultaneously...

Ngày tải lên: 11/04/2014, 00:19

... Rho GTPase activity in dendritic cells J Immunother 2008, 31(5):491-499 Shurin GV, Amina N, Shurin MR: Cancer therapy and dendritic cell immunomodulation In Dendritic Cells in Cancer Edited by: ... Shurin GV: Mechanisms of dendritic cell- induced T cell proliferation in the primary MLR assay Immunol Lett 2001, 78(2):75-82 Steinman RM, Witmer MD: Lymphoid dendritic cells are potent stimulators ... Optimizing dendritic cell- based immunotherapy for cancer Expert review of vaccines 2007, 6(3):333-345 Chao D, Bahl P, Houlbrook S, Hoy L, Harris A, Austyn JM: Human cultured dendritic cells show...

Ngày tải lên: 18/06/2014, 15:20

... noncoding B cell integration cluster (Bic) gene [23] and is functionally important in B cell, T cell and macrophage biology miR-155 is up-regulated in B cells exposed to antigen, in T cells stimulated ... generation of dendritic cells J Immunol Methods 2005, 298:61-72 11 Albert ML, Jegathesan M, Darnell RB: Dendritic cell maturation is required for the cross-tolerization of CD8+ T cells Nat Immunol ... expressed in human activated and mature dendritic cells through serial analysis of gene expression Blood 2000, 96:2206-2214 Young JW, Merad M, Hart DN: Dendritic cells in transplantation and immune-based...

Ngày tải lên: 18/06/2014, 16:20

... NC, USA) B16 mouse melanoma cell line and EL4 mouse lymphoma cell line were obtained from Shanghai Institute of Cell Biology and Biochemistry (Shanghai, China) All of cell lines were maintained ... Contassot E, Beermann F, Huard B, French LE: Tumor-infiltrating dendritic cells are potent antigen-presenting cells able to activate T cells and mediate tumor rejection J Immunol 2006, 176:61-67 ... an effective antitumor immune response is critically dependent on dendritic cells (DCs), the most effective antigen-presenting cells (APCs) that capture antigens in peripheral tumor tissues and...

Ngày tải lên: 18/06/2014, 16:20

... induced in vitro by CD16- and CD16+ monocytederived dendritic cells in patients with metastatic renal cell carcinoma treated with dendritic cell vaccines J Clin Immunol 2004, 24:86-96 Aricò et ... transmenbrane G-protein-coupled receptor CXCR3, and is expressed especially in activated Th1 cells, B cells, NK cells and DC, thus suggesting that CXCL10 release can represent a primary event in the ... (Wilcoxon Matched Pairs test) List of Abbreviations IFN: Interferon; DC: Dendritic Cells; PBMC: Peripheral Blood Mononuclear Cells; Th: T helper; CTL: Cytotoxic T Lymphocytes; HCV: Hepatitis C Virus;...

Ngày tải lên: 18/06/2014, 19:20

... 40-micron cell strainer to prepare single -cell suspensions This is a standard method of cell preparation and does not result in selective loss or enrichment of lymphocytes or dendritic cells Viabilities ... through a cell strainer to produce single -cell suspensions The cells were pelleted by centrifugation at 500g for minutes at 4uC, and then red blood cells were lysed by resuspending the cell pellets ... nodes through 40-micron cell strainers, and CD11c+ cells were isolated by magnetic bead separation The cells were stained for CD11b and separately for each of the dendritic cell activation markers...

Ngày tải lên: 08/08/2014, 21:20