2 neural stem cells and their manipulation

... inhibitor improves learning and working memory Behav Neurosci 2009;123:218-223. 4 adult neural stem cells after hypoxia/reoxygenation injury in mice Mol Cell Neurosci 2010;43:201-208. 5 neurogenesis ... markers P62 and LC3 in C17.2 cells (C) *p<0.05 and ***pδͲǤͲͳ˜•Ǥ…‘–”‘Ž‰”‘—’ȋȌǤ…ƒŽ‡„ƒ”αͷͲɊǤ Trang 812%, 61%, 49% and increased by 1% following fasudil treatment for 1, 3, 6, 12 and 24 hs, ... Science 2002;298:840-843. 21 differentiation via Notch pathway activation Neurosci Lett 2014;559:1-6. 22 Notch1 pathway: in search of the mediator(s) of neural stem cell differentiation PLoS One 2011;6:e14668....

Ngày tải lên: 02/11/2022, 10:39

... SOX2………… 24 3.3.8 NANOG 24 3.3.9 KLF4……… 25 3.3.10c-myc……… 26 3.3.11Lin28………… 26 3.4 Cardiac differentiation of Human IPS cell lines 27 3.5 Generation of Porcine Induced Pluripotent Stem Cells ... Fibroblast (MEF) cells as feeder cells for hESCs 37 4.2 Culture of Human Embryonic Stem Cells (hESCs) 38 4.2.1 Culture of Human Embryonic Stem Cells on MEF feeders 38 4.2.2 Feeder- free Culture ... 18 3.3.2 Episomal Reprogramming 20 3.3.3 mRNA reprogramming 21 3.3.4 Chemical reprogramming 21 3.3.5 Genes that regulate pluripotency in Human Embryonic Stem Cells……… 22 3.3.6 OCT4………23 3.3.7...

Ngày tải lên: 30/09/2015, 10:13

... therapy 21 1.3.5 Current status of gene therapy for glioma and breast cancer 22 1.4 Stem cells 23 1.4.1 Adult stem cells 24 1.4.1.1 Neural stem cells 24 1.4.2 Induced pluripotent stem cells ... cells 25 1.5 Stem cell as a vehicle for cancer gene therapy 26 1.5.1 Stem cell based cancer gene therap 26 Trang 51.5.2 Cancer tropism of stem cells 27 1.5.3 Route of administration of stem ... (elaborated in section 3.1) 1.4 Stem cells 1.4.1 Adult stem cells (ASCs) Adult stem cells (ASCs), also known as somatic stem cells, are multipotent undifferentiated stem cells found in a tissue or...

Ngày tải lên: 09/09/2015, 18:56

... for miR-22, miR-221, miR-21 and miR-145 in PAX6 3’ UTR 89 Figure 4.4 miR-22 and -221 possibly downregulate PAX6 by specific targeting of PAX6 3’ UTR 91 Figure 4.5 Effect of miR-22 and -221 on ... 80  4.1 Introduction and aims 80  4.2 Results 82  4.2.1  miR-22, -21, -221 and -145 are upregulated in GPCs compared to NSCs and are predicted to target PAX6 mRNA 82  4.2.2  PAX6 is a prospective ... neuronal cells by the expression of neural-specific transcription factors, including Sox2, Pax6 and Olig2 (Han et al., 2012; Lujan et al., 2012; Ring et al., 2012; Thier et al., 2012) These...

Ngày tải lên: 10/09/2015, 09:08

... hESC 21! 2.11! Applications of hESC research 22! 2.12! Neural differentiation of hESC 24! 2.12.1! Neural induction 25! 2.12.2! Neural subtype specification 26! CHAPTER 3 ! MATERIALS AND METHODS ... transduction 4! 2.2.1! SHH processing 4! 2.2.2! SHH pathway components 5! 2.2.3! SHH signal transduction 7! 2.3! SHH in embryogenesis 9! 2.4! SHH and neural development 10! Trang 52.5! SHH and proliferation ... glial cells, astrocytes, oligodendrocytes, neural crest stem cell cells and retinal cells (Bjorklund et al., 2002; Faulkner and Keirstead, 2005; Lamba et al., 2006; Lee et al., 2006; Lim et al., 2006;...

Ngày tải lên: 11/09/2015, 10:14

... development 16 1.6 Neural stem cells 19 1.6.1 Embryonic neural stem cells 20 1.6.2 Adult neural stem cells 24 1.6.3 Neural stem cell applications 26 1.7 Methods to study neural stem cells 28 1.7.1 Identifying ... 1.2 The stem cell concept 2 1.3 Symmetrical and asymmetrical division 6 1.4 Types of stem cells 7 1.4.1 Embryonic stem cells 7 1.4.2 Somatic stem cells 9 1.4.3 Stem cells and cancer 14 1.5 Neural ... progenitors .22 Figure 1.11 The SVZ niche, cell types and stem cell lineage 25 Figure 1.12 Neurogenesis in the adult rodent brain 29 Figure 1.13 Prospective isolation of stem cells and their progeny...

Ngày tải lên: 12/09/2015, 21:26

... proteins ···12 2.2.2 Notch signaling pathway ···14 Trang 82.2.3 bHLH transcription factors ···15 2.2.3.1 Hes1 and Hes5 ···15 2.2.3.2 Neurog1/2 and Ascl1 ···17 2.2.3.3 Olig1 and Olig2 ···19 3 Hyperglycemia-associated ... mechanisms of neural tube development···6 2.1 Development of the neural tube···6 2.2 Factors involved in neural tube development ···9 2.2.1 Morphogens···10 2.2.1.1 Sonic hedgehog···11 2.2.1.2 Bone ... NSCs and differentiated cells ···193 Figure 20 Olig1 mRNA expression in NSCs and differentiated cells ···196 Figure 21 Olig2 mRNA expression in NSCs and differentiated cells ···199 Figure 22 Intracellular...

Ngày tải lên: 30/09/2015, 06:36

... light on the role of neural stem cells as the transformational Trang 24glioma-cells in GBM formation, hence their accurate terminology as glioma stem glioma-cells or -initiating cells14, 16, 17 Traditionally, ... these cells in clinical specimens has followed the studies involving first, leukemic stem cells20 and now several other tumor systems21-24; namely, tumor-initiating capacity was defined as cells ... Tumor Neurospheres 24 2.14 Sorting of CD133+ and CD133- GPC Cell Populations 27 Trang 5RESULTS CONTRIBUTION OF STEM-LIKE GLIOMA-PROPAGATING CELLS TO MOLECULAR HETEROGENEITY AND SURVIVAL OUTCOME...

Ngày tải lên: 03/10/2015, 21:57

... of human cells 17 1.3.4 Zinc finger nuclease targeting 17 1.4 Aim of this study 19 2 Material 21 2.1 Technical equipment 21 2.2 Plastic ware 24 2.3 Chemicals 25 2.4 Enzymes 29 2.5 Restriction ... endonucleases 30 2.6 Cell lines and animals 30 2.7 Plasmids 31 2.8 Bacterial solutions 31 2.9 Cell culture media 32 2.10Cell culture solutions 33 2.11Cell culture stock solutions 35 2.12Molecular ... Reynolds and Weiss, 1992; Urbach et al., 2004) Neural stem cells of this process can give rise to neurons and glia, the two lineages most cells of the central nervous system belong to, and are...

Ngày tải lên: 26/11/2015, 09:54

... rats Brain Res 2008, 1231:103 –112. 5 Okano H: Stem cell biology of the central nervous system J Neurosci Res 2002, 69(6):698 –707. 6 Gottlieb DI: Large-scale sources of neural stem cells Annu Rev ... an atmosphere of 1% O2, 5% CO2and 94% N2, 98% humidity at 37°C The incubator was flooded with pre-warmed and humidified gas consisting of 5% (v/v) CO2 in 95% N2 Oxygen and CO2 content in the wells ... (EBSS) (g/L: NaCl 6.80, KCl 0.4, CaCl2 0.2, MgSO4 0.2, NaH2PO4 1.14, NaHCO32.2, phenol red 0.02) The cells were then (for 96-well plates) of glucose-free NBM-B27 media (Neurobasal glucose-free,...

Ngày tải lên: 02/11/2022, 11:35

... Microsurgery 2002, 22:311-315. 11. Heath CA: Cells for tissue engineering Trends Biotechnol 2000, 18:17-19. 12. Hori J, Ng TF, Shatos M, Klassen H, Streilein JW, Young MJ: Neural progenitor cells lack ... Trophism of neural progenitor cells to embryonic stem cells: neural induction and transplantation in a mouse ischemic stroke model J Neurosci Res 2007, 85:1851-1862. 9. Francel PC, Francel TJ, ... collagen-hyaluronan matrix J Neurosci Res 2007, 85:2138-2146. 4. Chu K, Kim M, Jeong SW, Kim SU, Yoon BW: Human neural stem cells can migrate, differentiate, and integrate after intrave-nous transplantation...

Ngày tải lên: 18/06/2014, 15:20

... 28 5, 28 7, 28 9, 29 0, 29 4, 29 7, 29 8, 300, 301, 307–313 Cdk6, 184, 23 2, 26 0 26 2, 27 3, 27 6, 27 8, 28 4, 28 5, 28 7, 29 0, 308–310 CDKN1A, 166 Cdks, 161, 27 1, 27 2, 27 5, 27 6, 27 8, 27 9, 28 1, 28 5, 300, 3 02, ... 187, 188, 1 92, 194, 199, 20 0, 20 6 20 9, 22 7, 23 1, 25 7 26 0, 26 2, 26 6, 27 3, 27 9, 28 2, 28 4, 29 1, 29 2, 29 4 29 7, 29 9, 309–3 12 – function of, 9, 22 – Rb knockout, 16 – regulation of, 10 Rbx1, 1 52, 156 redundancy, ... 159, 183, 21 1, 22 7, 25 9, 27 1, 27 2, 27 6, 29 4, 29 5, 29 7 29 9, 310, 311 carcinogen, 22 9 Cdc16, 1 52 Cdc18, 158 Cdc2, 23 0, 27 1 27 3, 27 5 28 0, 28 2, 29 3, 300, 301, 303–305, 307, 309, 3 12, 313 Cdc20, 93,...

Ngày tải lên: 25/10/2013, 21:20

... eye-glasses and a small tube near the mouth for inhalation.” Copyright 1996 Scientific American, Inc 50, 100 and 150 Years Ago NEWS AND ANALYSIS 24 44 52 SCIENCE TECHNOLOGY AND THE AND PROFILE ... Visuals I n 19 92 the Better Homes Fund, a nonprofit organization based in Massachusetts, began a study of 21 6 women in low-income housing and 22 0 homeless women, along with 627 of their dependent ... her abusive husband and also Sally and her two older brothers The family moved in with Sally’s paternal grandmother Sally’s father was an alcoholic, always in and out of jobs and, because he...

Ngày tải lên: 12/05/2014, 15:15

... Translational Medicine 20 08, 6:79 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 kinase Aurora A in spindle assembly Nat Cell Biol 20 03, 5(3) :24 2 -24 8 Cheeseman IM, Anderson S, Jwa M, Green ... Non-metastatic cells T-cell lymphoma invasion and metastasis Fold Change 15. 42 4.51 2. 73 2. 46 2. 26 2. 24 2. 18 Page of 13 (page number not for citation purposes) Journal of Translational Medicine 20 08, ... Clin Cancer Res 20 04, 10 (22 ):7439-7449 Orr GA, Verdier-Pinard P, McDaid H, Horwitz SB: Mechanisms of Taxol resistance related to microtubules Oncogene 20 03, 22 (47): 728 0- 729 5 Anand S, Penrhyn-Lowe...

Ngày tải lên: 18/06/2014, 15:20

... cells, cancer, and cancer stem cells Nature 20 01, 414:105-111 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 Ponnusamy MP, Batra Sk: Ovarian cancer:emerging concept on cancer stem cells Journal ... viability of A2780 and C200 ovarian cancer cells The viability of A2780 and C200 cells treated with genistein (10 25 μM), cisplatin (25 0 nM), gemcitabine (2 – 50 nM) and taxotere (1 2 nM) were determined ... were evaluated in the A2780 and C200 cells PARP cleavage was determined in A2780 and C200 cells that were treated with genistein (25 μM), cisplatin (25 0 nM), taxotere (2 nM), gemcitabine (50...

Ngày tải lên: 20/06/2014, 07:20

... http://www.ovarianresearch.com/content/3/1/11 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 Herzog TJ, Pothuri B: Ovarian cancer: a focus on management of recurrent disease Nat Clin Pract Oncol 20 06, 3:604-611 Alvarez ... statistics, 20 09 CA Cancer J Clin 20 09, 59 :22 5 -24 9 Armstrong DK: Relapsed ovarian cancer: challenges and management strategies for a chronic disease Oncologist 20 02, 7(Suppl 5) :20 -28 Markman M: ... cisplatin A2780CP cells were treated as indicated for a total of 48 hrs and protein lysates were collected and analyzed by immunoblotting for caspase and PARP (D) Bands for full length and cleaved...

Ngày tải lên: 20/06/2014, 07:20

... Ovarian Research 20 10, 3:13 http://www.ovarianresearch.com/content/3/1/13 15 16 17 18 19 20 21 22 23 24 25 26 expressed in the normal human breast and in breast cancer Med Oncol 20 04, 21 : 123 -31 Wente ... USA and 2Department of Anatomy and Cell Biology, Temple University School of Medicine, Philadelphia, PA, USA Received: 22 September 20 09 Accepted: 26 May 20 10 Published: 26 May 20 10 © 20 10 Rough ... Caspase-3 and -7 Assay Kit (V35118, Molecular Probes, Eugene OR) Anti-p27 (sc- 528 , 1 :20 0), anti-BAX (sc-7480, 1 :20 0), anti-caspase precursor (sc-7148, 1 :20 0), anti-LXRα (sc- 120 2 1 :20 0), anti...

Ngày tải lên: 20/06/2014, 07:20

... resistance transporter ABCG2 (breast cancer resistance protein 1) effluxes Hoechst 333 42 and is overexpressed in hematopoietic stem cells Clin Cancer Res 20 02, 8 :22 -28 16 Ferrandina G, Bonanno G, Pierelli ... Muc4-ErbB2 complex formation and signaling in polarized CACO -2 epithelial cells indicate that Muc4 acts as an unorthodox ligand for ErbB2 Mol Biol Cell 20 06, 17 :29 31 -29 41 doi:10.1186/1757 -22 15-4-7 ... KD: ABC transporters as phenotypic markers and functional regulators of stem cells Stem Cells 20 02, 20 :11 -20 Ponnusamy et al Journal of Ovarian Research 20 11, 4:7 http://www.ovarianresearch.com/content/4/1/7...

Ngày tải lên: 20/06/2014, 07:20

... brain aging and Alzheimer's disease are mirrored in experimentally silenced cortical neuron networks Neurobiol Aging 20 12, 33 :20 5 e201 -20 5 e218 15 13 14 15 16 17 18 19 20 21 22 23 24 Cheadle C, ... cancer cell lines Clin Cancer Res 20 03, 9 :27 78 -27 85 16 25 26 27 28 29 30 31 32 33 Frisch SM, Screaton RA: Anoikis mechanisms Curr Opin Cell Biol 20 01, 13:555-5 62 Sherman-Baust CA, Weeraratna AT, ... SPOCK2 NINJ2 THBS1 SOX21 CD44 RGS4 DDIT4 IGF2 GPC3 PRSS8 APOC1 ITIH2 MAF FABP5 IGSF4 SOX21 NPC2 SCD MT1F RRAGD SPOCK2 RENBP SPINT2 RFTN1 TCN1 PRNP FKBP11 MSMB LCP1 NNMT MAF ECHDC2 ANKRD38 WDR72...

Ngày tải lên: 20/06/2014, 08:20

... TF and cap cells (Lopez-Onieva et al., 20 08) and stat92E-gfp is expressed in TF cells, cap cells, ESCs, and ECs (Decotto and Spradling, 20 05; Lopez-Onieva et al., 20 08) 14 STAT92E and Hop, when ... Differentiation in IGS cells 98 4.1 .2. 2 Discussions 100 4 .2 Cell identity of Extra Spectrosome-Containing Cells in med20RNAi Germaria.1 02 4 .2. 1 Introduction 1 02 4 .2. 2 Results: Transient ... embryonic stem cells and adult stem cells The embryonic stem cells are totipotent, and this means that they can differentiate to all types of cells, which constitute an entire organism Adult stem cells...

Ngày tải lên: 14/09/2015, 08:42