... used for comparative studies to find the appropriate basal nutrient medium for the further formulation of the optimal medium Optimisation of fermentation conditions The optimum conditions for ... all the reagents but the reaction was terminated prior to the addition of enzyme extract One unit of xylanase activity was defined as the amount of enzyme catalysing the release of 1 μmol of reducing ... death phase and in the long lag phase of the fermentation profile Effect of salinity The effect of salinity on xylanase production was studied by preparing the respective production media with...
Ngày tải lên: 14/01/2020, 13:36
... 1 gm of the food sample Dilutions were made from 10-1 to 10-6, these dilutions were used in the plating for the isolation of micro-organisms Spread plate method was used for isolation of the bacteria, ... the help of a swab After 5 minutes of incubation, 6 mm diameter well was punched in the plates with the help of sterile cork borer, 80 μl of the inoculum of the test organism was added into the ... Optimization of glucose concentration for the antimicrobial activity of Bacillus firmicutes against the food borne pathogens Fig.4 Optimization of maltose concentration for the antimicrobial activity of...
Ngày tải lên: 14/01/2020, 03:53
Tài liệu Novel Design of an Integrated Pulp Mill Biorefinery for the Production of Biofuels for Transportation pot
... beginning of the process to soften the incoming wood chips Next, the same process as the refiner pulping is completed to produce the pulp Compared to the other mechanical processes, this is the most ... because of the presence of an inert liquid medium and better mixing The second and the third problems are related to the acidity of the dehydration catalyst in a dual catalyst system Therefore, ... intermediate The first part of the reaction from synthesis gas to methanol is quite exothermal and it is limited by equilibrium at a fairly low temperature Therefore, the first part of the reaction...
Ngày tải lên: 26/02/2014, 14:20
Cocoa Processing Methods for the Production of High Quality Cocoa in Vietnam ppt
... storage on the fermentation temperatures of cocoa 5: The effect of pod storage and fermentation time on the percentage of fully brown beans in the cut test 6: The effect of spreading of beans for two ... Methods for the Production of High Quality Cocoa in Vietnam 1 Trang 2Table of ContentsAcknowledgements List of figures List of Illustrations List of plans Harvesting of pods Storage of pods ... the closer to 50oC, that fermentations reach, the better the quality of the dried cocoa is Typical temperatures for the different types of fermentation are presented in figures 5, 6 & 7 For...
Ngày tải lên: 21/06/2014, 06:20
Báo cáo khoa học: " Effective methods for the production of immunoglobulin Y using immunogens of Bordetella bronchiseptica , Pasteurella multocida and Actinobacillus pleuropneumoniae" docx
... has been the focus of attention in immunotherapy and immunodiagnosis, since the birds can actively transfer immunoglobulin G (IgG) to the egg yolks of their offsprings in the same levels of concentration ... volume of PBS The homogenized egg yolk was then mixed with two volumes of chloroform and incubated for 2 hrs at room temperature After this incubation, the supernatant was collected To compare the ... 27, 30) The use of chicken antibody for large scale production of antibodies to mammalian pathogens offers several advantages such as; animal welfare, low costs in production and safety for dangerous...
Ngày tải lên: 07/08/2014, 15:20
Microencapsulation of clostridium acetobutylicum cells and utilisation of samanea saman leaves for the production of biobutanol
... page of my entire thesis as I list the names of all the people who have, in some way or the other, helped me reach the end of the scientific adventure that I ventured four years back First and foremost, ... substrate for biobutanol production, was attempted The combination of these methods were aimed to address the issues of low butanol yield and high production cost of biobutanol production The factors ... subsequently form the forespore cells (mother cells containing the endospores) The cells in the stationary phase are involved in conversion of acids to solvents Finally, the endospore is released from the...
Ngày tải lên: 08/09/2015, 18:41
Catalytic dry reforming of natural gas for the production of chemicals and hydrogen
... by use of the large heat of reaction and the reversibility of this reaction system [2,3] One of the major problems encountered in the application of this process is rapid deactivation of the catalyst, ... characteristic of the forms of coke observed on nickel catalysts for CO2 reforming (iii) After 20 h exposure to the reforming stream, either under di5erential or integral conditions, most of the Ni parti-cles ... rate of the initial state to a certain extent, it does not inNuence sig-niDcantly the value of the reaction rate at the stable level These results imply that there exists a strong tendency of the...
Ngày tải lên: 03/04/2018, 20:27
Separation, extraction and concentration processes in the food, beverage and nutraceutical industrie
... (23) The area enclosed by the solvent circuit in the TS diagram represents the thermodynamic work needed for the process of cycling the SCF The lower the pressure drop needed for separating the ... critical point of the other component and then rising sharply with pressure, whereas the other line goes from the critical point of the other component to the UCEP Various sub-classes of type III ... mixture of compounds has to be high whereas in another stage, the solubility of a compound in SCF has to be low Therefore, the phase equilibrium data is the most important factor in the design of the...
Ngày tải lên: 20/09/2015, 01:57
Báo cáo khoa học: Apoptosis-inhibiting activities of BIR family proteins in Xenopus egg extracts ppt
... important for caspase inhibition Some members also contain the C-terminal RING finger domain, which is reported to be required for the ubiquitylation of themselves and bound caspases [26–28] They are therefore ... (Fig 3B) However, these proteins may be unstable and completely degraded before the onset of apoptotic change To examine the stability of these proteins, we first tried to detect the endogenous molecules ... be further degraded by the N-end rule pathway in Drosophila [43] To evaluate the contribution of these proteolytic systems, the effects of specific protease inhibitors were tested against the degradation...
Ngày tải lên: 30/03/2014, 16:20
Báo cáo y học: "Eggshell and egg yolk proteins in fish: hepatic proteins for the next generation: oogenetic, population, and evolutionary implications of endocrine disruption" pptx
... silencing of at least nine of the ten pseudogenes (ibid.) It has become increasingly clear that the proteins of the zona pellucida are conserved among eutherian mammals and that the proteins of the ... body) Towards the end of the vitellogenic period, or by the beginning of the final maturation, the germinal vesicle (nucleus), which in the early stages is centrally located, moves to the periphery ... differences in the structure of zona radiata interna [65] Since 1989, several reports have demonstrated the hepatic synthesis of precursor proteins of the inner layer subunits under the influence of estrogen,...
Ngày tải lên: 13/08/2014, 13:20
Comparison of Methods for the Extraction of Bioflocculants from Activated Sludge
... of 95 ml) The A280 to A260 ratio of the UV-absorbing peak indicated it was composed mainly of nucleic acid In the profile of the NaOH-extracted polymer (B), the elution volume (80 ml) of saccharide ... control test was calculated every minute using the following formula: The relative A660 = A660 of flocculating test A660 of control test For the control, the polymer solution was replaced by distilled ... compared to the other methods The SDS-extracted polymer included a small amount of material other than protein Furthermore, the steaming- and washing-extracted polymers contained large amounts of phosphorus...
Ngày tải lên: 05/09/2013, 09:08
Tài liệu Báo cáo khoa học: A knowledge-based potential function predicts the specificity and relative binding energy of RNA-binding proteins ppt
... on the presence or absence of a cytosine at position )5 in the RNA A likely explanation for the importance of the )5 cytosine mutation is offered by the observation that the amino group of the ... onto the RNA bases nearest the center of the b-sheet structure of the RRM We then scored these sequence-variant structures with the distancedependent potential function Figure shows the results of ... the highresolution modeling of protein–RNA interactions The anticipated future enrichment of the structural database will further improve the predictive performance of the potential Results The...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: Structural bases for recognition of Anp32⁄LANP proteins doc
... accurate definition of the C-terminal domain boundary Experimental Study of the interactions of the Anp32a LRR module determination of the dynamic features of the domain in solution, together with a ... within the range expected for a single monomeric species of this size in solution [29] The flat profile of the relaxation parameters along the sequence indicates that, with the exception of the first ... Study of the interactions of the Anp32a LRR module C de Chiara et al A B Fig Modeling the interactions of the Anp32a LRR domain (A) Structure of the Anp32a LRR domain in a complex with the C...
Ngày tải lên: 18/02/2014, 17:20
Tài liệu Báo cáo khoa học: Binding of N- and C-terminal anti-prion protein antibodies generates distinct phenotypes of cellular prion proteins (PrPC) obtained from human, sheep, cattle and mouse doc
... used for PrP detection Calculation of the banding patterns of 17 gels using antibody SAF34 gave an SE of 2.1 for the di-glycosylated isoform, 1.0 for the mono-glycosylated band and 1.6 for the ... calculation of the percentages of the di- (d), mono- (j) and nonglycosylated (m) isoform as arithmetic means of separate gel runs The number of gel runs for the analyses are given for each antibody ... (calculated here for murine PrP) [18] Taken together, the results of various signal intensities of the three PrPC bands are accredited to the development of the truncated isoforms, to the epitope...
Ngày tải lên: 19/02/2014, 02:20
Tài liệu Báo cáo khoa học: Analysis of oxidative events induced by expanded polyglutamine huntingtin exon 1 that are differentially restored by expression of heat shock proteins or treatment with an antioxidant ppt
... of the inclusions was noticed Moreover, the percentage of cells presenting inclusions as well as the distribution of the size of the inclusions were dependent on when the analysis was performed ... (NAC) before being analyzed The arrow indicates the position of the more intensively oxidized polypeptide in the assay The bracket underlines the domain (molecular mass range 10–40 kDa) of the oxyblots ... problem that the cell has to challenge to promote the formation and clearance of these bodies Aggregate clearance could therefore be balanced between the rate of huntingtin aggregation and the deleterious...
Ngày tải lên: 19/02/2014, 06:20
Tài liệu Báo cáo khoa học: Subproteomics analysis of Ca2+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle pdf
... to the Dp427 isoform of dystrophin (A), the a-subunit of the dystroglycan complex (a-DG; B), the Na+/K+ ATPase (NKA, C), the fast SERCA1 isoform of the sarcoplasmic reticulum Ca2+ ATPase (D), the ... with both the 2D ÔStainsAllÕ analysis of Fig and the 1D immunoblotting of Fig As the full-length Dp427 isoform of dystrophin does not enter the second dimension of conventional 2D gels, the expression ... In contrast, the expression of major ion-regulatory muscle components, such as the Na+/K+ ATPase, the SERCA1 isoform of the sarcoplasmic reticulum Ca2+ ATPase, and the a1-subunit of the dihydropyridine...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: "Cross-Domain Co-Extraction of Sentiment and Topic Lexicons" pdf
... experiments to study the effect of different parameter settings There are several parameters in the framework: the number of generated seeds r, the number of new candidates k2 and the number of selections ... word extraction, the results of the proposed methods are not sensitive to the values of r While for the topic word extraction, the proposed methods perform well when r falls in the range from 15 ... trees for the sentence the camera is great” in the camera domain and the sentence the movie is excellent” in the movie domain, respectively As can be observed, the relationships between the topic...
Ngày tải lên: 19/02/2014, 19:20