2 in vitro engineering of retinal cell types

... The impact of radiation quality on chondrosarcoma cells cultivated in 3D was analyzed on cell death, cell proliferation and DNA repair Results: A fair distribution of chondrosarcoma cells was ... ARTICLE Open Access In vitro engineering of human 3D chondrosarcoma: a preclinical model relevant for investigations of radiation quality impact Dounia Houria Hamdi1, Sofia Barbieri1,2, Franỗois ... correlated DNA damages within one cell occur more often so that it becomes more difficult for the cell to repair the damage, leading to a markedly increased efficiency of cell © 2015 Hamdi et al Open

Ngày tải lên: 28/09/2020, 01:29

... comprised the preparation of 1–2 mm tumor fragments with a sterile blade and placing of three to six pieces onto one well of a six well cell culture dish (patients 03–09) Primary cell culture medium ... 200μl (120 μl) of the cell-Matrigel suspension were placed on the Matrigel coated cell culture dishes and incubated for 30 min at 37 °C For the first passage, a cell-Matrigel ratio of 1:3 was used ... examination Establishment of two-dimensional cell cultures and PDAC organoids Primary cell cultures from tissue samples of 14 patients with PDAC could be initiated and 11 primary cell cultures could

Ngày tải lên: 30/05/2020, 21:42

... Purity of sorted CD133 lo and CD133hicells was recorded as >90% Cell cycle analysis of the total Y79 cells, CD133loand CD133hisubsets highlighting the G0/G1 status of CD133 lo cells (l) Cell ... and the cells were sub-cultured following observation of cell confluency of about 70% Enrichment and characterization of putative cancer stem cells were carried out in the cultured Y79 cells and ... the in vitro differentiated cells [21] The evidence from retinal studies and in-creasing expression of CD133 in long-term cultures of Rb supports our observation of CD133 as a marker of differentiation

Ngày tải lên: 06/08/2020, 03:05

... pharmacological inhibition of MMPs re-duces Rb cell viability, migration, and secretion of the pro-angiogenic factors VEGF and Angiopoietin-2 in either one or both types of Rb cell lines These promising ... impact in the prognosis of Rb To investigate the effects of inhibition of MMP-2 and MMP-9 on the migration of Rb cells we used both a metastatic model represented by the Y79 cell line and a non-metastatic ... suggesting this is not an off-target effect of downregulation of the MMP-2 and MMP-9 Pharmacological inhibition of MMPs reduces secretion of angiopoietin-2, but not VEGF, in Y79 cells Retinoblastoma

Ngày tải lên: 06/08/2020, 07:35

... pathogenesis of lung cancer To validate the hypothesis of HPV involvement in small cell lung cancer pathogenesis we performed a gene expression profile of transgenic mouse model of SCLC induced ... Lung cancers consist of four major types that and for clinical-pathological reasons are often divided into two broad categories: small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) ... The quality of labelled targets was determined by calculating the amount of cDNA produced, the pmoles of dye incorporated and the frequency of incorporation by NanoDrop Equal amounts of cRNAs (825

Ngày tải lên: 18/06/2014, 16:20

... The distinctive feature of different stem cell types is based on the capability of the cells to differentiate along multiple lineages and produce derivatives of cell types of the three germ layers ... differentiate in cell types of all three Can differentiate in cell types of all three germ germ lineages lineages Can differentiate in limited cell types depending on the tissue of origin Ability ... Limited lifespan (population doublings) Production of endless number of cells Production of endless number of cells Production of limited number of cells Chromosome length is maintained across serial

Ngày tải lên: 18/06/2014, 19:20

... function of tumor cell apoptosis and not abso-lute number of T cells at the tumor cell layer, since T cells may themselves undergo apoptosis after inducing tumor cell apoptosis and one T cell may ... Abs and excess CCL2a Blocking agent Number of tumor cells/ field (10 fields) Number of apoptotic cells/ field (10 fields) % of apoptotic cells % of tumor cell apoptosis inhibition None 21.8 ± 9.7 ... II) after adding T cells Day of harvesting of reconstruct cultures was based on optimum constriction of the collagen gel of reconstruct cultures The ratio of apoptotic tumor cells was determined

Ngày tải lên: 18/06/2014, 19:20

... increases OS in Vero cells Se is an integral part of the active site of GPx1, an enzyme that protects cell damage by reducing intracellular H 2 O 2 In vitro response of Vero and SK-N-SH cells to Se deficiencyFigure ... SK-N-SH cells at day 7 of the induction of Se deficiency was assessed by cell proliferation assay and percentage cell viability of Se- and Se+ cells was calculated by comparing to control cells. ... of cells were seeded in 96-well plates and growth curve was measured by cell counting of control, Se-, and Se+, Vero (A) and SK-N-SH (B) cells for 5 days post-seeding. (C) Cell viability of

Ngày tải lên: 20/06/2014, 01:20

... apoptosis cells × 100)/total number of cells Morphology of the cells was studied by using aceto-orcein dyes that can separate mitotic cells from interphase cells Then the percentage of mitotic cells ... 100)/total number of cells For measurement of cell apoptosis, cells were seeded into 8-well chamber slides (300 μl cell suspension/well) When confluent, cell survival was assessed by staining cell nuclei ... (indicative of nonviable cells) versus stain-excluding cells were counted in a hemocytometer Then the percentage of viable cells was calculated as follows: viable cells (%) = (total number of viable cells

Ngày tải lên: 20/06/2014, 04:20

... competing ligand-dependent effects on the rate of Cu release (see Discussion) In vitro toxicity of CuCNPs in HeLa cells Thein vitro toxicity of CuCNPs in HeLa cells (a cervi-cal adenocarcinoma) was investigated ... is promising in terms of the stability of solu-tions of CuCNPs over long periods of time, Cu release cannot simply be a function of pH but must also depend on the presence of ligands that can compete ... (BioTek, Winooski, VT, USA) The viability of the cells exposed to particles was expressed as a percentage of the viability of cells grown in the absence of particles on the same plate Additional

Ngày tải lên: 21/06/2014, 02:20

... collection An average of 2.3 immature Trang 8oocytes were collected and an average of 1.7 matured in vitro Six of the eight women underwent embryo transfer of 1–3 embryos (average of 1.7); one patient ... the agents used for treatment of many types of cancer are successful in up to 95% of patients, they unfortunately carry a considerable risk of causing the loss of future fertility potential Because ... doses of gonadotropins They also experience a high cancellation rate because of the smaller number or size of follicles Many different ovarian stimulation protocols have been tried for treatment of

Ngày tải lên: 05/08/2014, 16:20

... indicator of in vitro culture dysfunctioning. The aim of this study was to compare the effects of 2 gelling agents, Difco Bacto Agar® (Difco) and Gelrite® (Kelco) on the growth of in vitro ... behavior of excreted substances in the 2 gelling agents, a reten-tion of growth substances by the agar, or even a breakdown of callus cells related to one or both of the above events Inhibition of ... content of leaves The mineral content of the leaves varied according to the gelling agent and the peri-od of time in culture A significant accumu-lation of Na was found in the leaves of the

Ngày tải lên: 08/08/2014, 23:22

... 1 Cell viability Cell viability was investigated with a Vi-Cell™ XR Cell Viability Analyzer (Beckman Coulter) to determine whether hypoxia at the applied conditions might cause cell death Cell ... time periods of more than 3 h for equilibration of pO2 between the medium inside the plate and the gas out-side of it, which even accelerated in the presence of cells [8], evaluation of both systems ... hypoxia chamber showed abso-lute cell numbers of about 70% and 90% of the absoabso-lute cell number after 24 h cultivation under normoxia Figure 3 Pathway analysis of hypoxia regulated genes using

Ngày tải lên: 09/08/2014, 09:20

... those of healthy donors, we compared these cells for their expression of co-stimulatory and functional markers In addition, the effect of cryopreservation of PBMC precursors on the quality of MDDC ... and HLA-DR, intracellular expression of IL-12 and COX-2, secretion of inflammatory cytokines, and proliferation of allogeneic and antigen-specific autologous T cells stimulated in vitro by DC. Defective ... frequency of IL-12+ and COX-2+cells, compared to the corresponding unstimulated cells (p < 0.05, data not shown) The dot plots in Fig 3A and 3B show the intracellular staining profiles of IL-12

Ngày tải lên: 11/08/2014, 10:23

... (CPE) (rounding of cells, loss of con-tact inhibition and cell death), after which the flasks were stored at -80°C for 24 hours Following two cycles of the freeze-thaw, the contents of the flasks ... adhering to cells, or by blocking the cellular receptors that are utilized by the virus to enter the cells This reduction of viral infectivity was determined by a reduced number of viral plaque ... significantly higher amount of inhibition of VSV plaque formation than HSV-1 plaque formation, in Vero cells This phenomenon may be attributed to the nature of the envelope proteins of rhabdoviruses When

Ngày tải lên: 12/08/2014, 04:20

... DEVELOPMENT OF A BIOREACTOR FOR IN -VITRO ENGINEERING OF SOFT TISSUES KYAW MOE (B.Eng (Hons.), YTU, Yangon) A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF ENGINEERING DEPARTMENT OF MECHANICAL ENGINEERING ... tissue -engineering approach to tissue repair and regeneration is the availability of appropriate cells The presence of cells is crucial; this is because of their proliferation potential, cell- to -cell ... water and cells [Akeson et al, 1984] Water makes up about 55% of wet weight of tendons and 60-80 % of wet weight of ligaments Collagen is arranged in the form of fibers within a matrix of GAGs,...

Ngày tải lên: 04/10/2015, 15:46

... Intensity of cytoplasm Ratio of Translocation = Intensity of nucleus Intensity of cytoplasm Intensity of nucleus Scheme This is a simplication of a cell as seen by analysis software, where the software ... not just in vitro and cell based but also simulated in silico Combining this method of analysis (in vitro, cell- based and in silico analysis) will facilitate systematic understanding of the underlying ... in single cell studies In our cell- based experiments, a population of 10 000 A549 cells was observed to undergo similar oscillatory behavior to that discovered in single cells in terms of the peak...

Ngày tải lên: 16/03/2014, 11:20

... http://www.translational-medicine.com/content/7/1/2 Figure Characterization of T cell responses Characterization of T cell responses (A) CD8+CD107a+ effector cells, (B) CD8+perforin+ effector cells, (C) PD-1+ T cells, (D) CD4+CD25+FoxP3+ Tregs ... titer ≥ fold of the baseline titer Page of 11 (page number not for citation purposes) Journal of Translational Medicine 2009, 7:2 T cell responses The IFN-γ ELISPOT was used to monitor T cell responses, ... from the cell membrane and thus can mediate antibody-dependent cell- mediated cytotoxicity (ADCC) In addition, 5T4-transduced renal carcinoma cell lines can be recognized by human T cells in vitro, ...

Ngày tải lên: 18/06/2014, 15:20

... flasks At least two different cell line have been used Accumulation of cAMP was measured in cells grown to confluence in 12-well plates and serum-starved for 24 h Cells were incubated at 37°C ... rings were passively sensitized by using a pool of sera containing high levels of specific IgEs but low levels of total IgEs With this method of passive sensitization and allergen challenge, ... 50% of active force in Salbutamol concentrations inhibiting 50% of active force in carbachol-contracted human bronchial rings Effects of montelukast on salbutamol concentrations inhibiting 50% of...

Ngày tải lên: 12/08/2014, 16:20