2  hifs in bone regeneration and repair

... + metformin: Improve insulin  Insulin + metformin: Improve insulin sentivity  Insulin -> metformin monotherapy  Reduce insulin dose 30 – 50% AND  Increase metformin dose  Usually successful ... TREATMENT Insulin Insulin Trang 17 Subsequent treatment - Failing targer HbA1c within 3-4m on metformin alone, addition of basal insulin - If metformin and basal insulin (up to - If metformin and ... 14Metformin Mechanism of Action Metformin Trang 15TREATMENT TREATMENT Insulin Insulin  Basal insulin ◦ Begin with 0.25 – 0.5 IU/kg/day ◦ Titrate to max 1.2 IU/kg/day  Insulin + metformin: Improve

Ngày tải lên: 19/10/2017, 23:44

... nuclear protein 1 - homeodomain - interacting protein kinase - 2) and TP53INP1- PKCδ (tumor protein 53 - induced nuclear protein 1 - δ protein kinase C) , phosphorylated protein 53 at serine - 46 ... binding ligand to HER initiates pathways of intracellular signal transduction When linked with other members called mating pairs Ligand will be mounted between chain I and III to release chain ... countries, America and increasingly tends to increase , especially in Asia CRC has become the concern of the public in general and for physicians specializing in Digestive system diseases in particular

Ngày tải lên: 15/01/2020, 23:08

... other laminins Upon injury, the high-abundance laminin chains were downregulated at day 3 and day 14 together with the collagen type IV chains In contrast, the laminina1-, a2-, and a4-chains were ... using unsupervised hierarchical clustering into early and late factors in repair (Fig 5A) This analysis revealed inter-esting heterogeneous regulation of interacting proteins, such as for instance ... combinations based on their MS intensity (Fig EV5A) The a3- and a5-laminins were most abundant in adult mouse lung, followed bya4-laminins Thea1- and a2-laminins, which are mainly restricted to embryonic

Ngày tải lên: 19/03/2023, 15:10

... 1CHAPTER 1 LITERATURE REVIEW AND INTRODUCTION Tissue engineering is the combination of cell, engineering and material methods, and application of suitable biochemical and physio-chemical factors ... plays an important role in the development of bone and cartilage It is involved in the Hedgehog pathway, TGF-beta signaling pathway, and the Cytokine-cytokine receptor interaction 1.2 Polymeric ... example, BMP-4 and its inhibitors noggin and chordin help regulate polarity of the embryo (i.e back to front patterning) Mutations in BMPs and their inhibitors (such as sclerostin) are associated

Ngày tải lên: 14/09/2015, 08:41

... healing without transferring bone tissues Among this group of proteins, BMP-2 has been shown to induce healing in segmental bone defects Aebli and colleagues (Aebli et al., 2005) and Saito and ... Scherping et al., 1997; Thaller et al., 1993) In particular, BMPs are osteoinductive proteins originally identified in demineralized bone (Urist and Nogami, 1970) They are known to facilitate bone ... factor, fibroblast growth factor and insulin growth factor have been shown to stimulate bone growth, collagen synthesis, and fracture repair both in vitro and in vivo (Jingushi et al., 1995; Nixon

Ngày tải lên: 14/09/2015, 08:41

... syringe, Popper & Sons, Inc., New Hyde Park, NY US) fitted with a 29-g needle and set up in the elecontrospinning apparatus The flow rate of polymer solution from the syringe into the spinneret ... investigating the effects of HAp content and the different methods of DNA loading on the physical and Trang 3biological characteristics of the micro-fibers fabricated using the electrospinning ... amounts of protein because of its short half-life Gene transfection is a powerful and promising alternative that involves the in vitro or in vivo incorporation of exogenous genes into cells for

Ngày tải lên: 14/09/2015, 08:41

... cytoclasts and correspondingly high bone healing rate For example, B3 displayed the highest ALP level in the second week, implying that the cytoclast differentiation rate and bone healing rate in B3 ... was clearly shown by white arrows in micrographs In contrast, those from A1 and B1 showed wide and dull bone ends, indicating the new formation of bone after two and four weeks This was more evident ... in bone regeneration in vivo Figure 6.1 Illustration of three plasmid loading modes in the present work Trang 36.2 Materials and methods 6.2.1 Materials chitosan (medium molecular weight and

Ngày tải lên: 14/09/2015, 08:41

... gradually and give 3-dimensional structural guide in the formation and regeneration of tissue (Goldstein et al., 2001).Therefore, combining the concept of gene delivery and tissue engineering, porous ... rpm for 5 min at 4 °C, and the supernatant was carefully collected and kept in the ice The total DNA intensities in all foams were determined by Trang 10PicoGreen dsDNA quantitation kit and compared ... determine as the morphology is highly different Moreover, in F0 and F1, some pores are isolated from other pores But in F2, all pores are open and interconnected in structure Table 7.1 shows the initial

Ngày tải lên: 14/09/2015, 08:41

... protein is too low, especially in the initial stage (Figures 7.5a and 7.7b) Therefore, an initial and significant release of DNA is demanded in order to optimize this kind of devices Lysine, ... structures, tunable pore size and well-defined surface properties of these materials allows the incorporation and release of a diversity of proteins and DNAs in a more reproducible and predictable manner ... determined using a Thermo plasmid DNA, different kinds of foams (F0, F1 and F2) were introduced into 0.5 mL of TE buffered solution of DNA (200 μg/mL) and soaked for 24 h under constant stirring

Ngày tải lên: 14/09/2015, 08:41

... binding protein heterodimer in damage recognition, because the complex binds damaged DNA with high affinity [43] and can dramatically increase the repair rate of certain DNA adducts, including ... excision repair gene involved in group A xeroderma pigmento-sum and containing a zinc-finger domain Nature 1990; 348: 73-6 65 Sarasin A An overview of the mechanisms of mutagenesis and carcinogenesis ... environmental and endogenous carcinogens Most of these alterations, if not repaired, can result in genetic instability, mutagenesis and cell death DNA repair mechanisms are important for maintaining DNA integrity

Ngày tải lên: 31/10/2012, 14:59

... Creating mental linkages B Applying images and sounds C Reviewing well D Employing action II Cognitive A Practising B Receiving and sending messages strategies C Analysing and reasoning D Creating ... for input and output III Compensation strategies A Guessing intelligently B Overcoming limitations in speaking and writing INDIRECT STRATEGIES I Metacognitive Strategies A Centering your learning ... contribute indirectly to learning since they do not lead directly to the obtaining, storing, retrieving, and using of language.” (Rubin & Wenden, 1987:23-27) Trang 15O’Malley and Chamot’s

Ngày tải lên: 29/01/2014, 00:23

... cystein, methionine and serine (Fig 2) Mutants containing asparagine, aspartic acid, glycine, lysine or valine were nonfunctional (Fig 2) Clones expressing eEF2 in which the adjacent threonine ... mito-gen-activated protein (MAP) kinase and mTOR-signal-ling pathways [17] These signalmTOR-signal-ling pathways activate the eEF2 kinase in response to mitogens and other stimuli that increase the cellular ... to bind to ribosomes in cells also expressing wild-type eEF2 from one of the remaining eEF2 coding genes Because binding of eEF2 to the ribosome is dependent on the preforma-tion of a guanosine

Ngày tải lên: 07/03/2014, 05:20

... a protein is dependent on the opposing activities of kinases and phosphatases Among the phos-phatases, protein phosphatase 2A is a major serine/threo-nine protein phosphatase involved in many ... expected initiation methionine by translating the fusion protein GST-XN73 and His6-XN73 As can be seen in Fig 2B, these fusion proteins run at the expected height, proving that no internal methionine ... gov/blast/) and the
MULTALIN alignment program (http:// prodes.toulouse.inra.fr/multalin/multalin.html) Human genome sequences were obtained by doingBLASTsearches against the HTGS database and the

Ngày tải lên: 08/03/2014, 08:20

... observed as strong punctate staining in intestinal cells and in the epidermis The number and intensity of fluorescent structures increased strongly in the intestine during larval develop-ment (Fig ... expressed in adipose tissue and interact with adiponec-tin Adiponectin is an adipokine specifically secreted from adipose tissue, which plays a key role in glucose and lipid metabolism in insulin-sensitive ... RCE1182) In brief, control and RNAi strain cultures were grown for 6 h in LB medium containing 100 mgÆmL)1 ampicillin, and then spread onto NGM agar containing isopropyl thio-b-d-galactoside (1 mm) and

Ngày tải lên: 16/03/2014, 03:20

... TSK-GEL HW75-F, and each fraction was examined by Western blotting with the anti-H-chain, the anti-L-chain, or the anti-fhx/P25 Ig (Fig 1C) Protein complexes containing H- and L-chains, and fhx/P25 ... fibroin [1], which consists of six sets of heavy chain (H-chain; 350-kDa fibrous protein)–light chain (L-chain; 26 kDa) disulfide-linked heterodimer and one molecule of a glycoprotein, fibrohexamerin ... excess of cocoon protein samples was used from Nd-sDand the transgenic line L7-4 in order to obtain similar signal intensity In all the cocoon protein samples, H-chain (Fig 5A) and fhx/P25 (Fig 5C)

Ngày tải lên: 16/03/2014, 16:20

... of findings suggest that BMP-2, -4, and -7 work to promote fracture healing and bone regeneration (81) Osteonectin is one of many extracellular matrix proteins involved with bone repair and regeneration ... (Figs and 2A,B) The process of mineralization in avian bone is associated with the elaboration of various phosphoproteins, including an approx From: Bone Regeneration and Repair: Biology and Clinical ... 30 Sfeir et al kinase activity, stimulating mesenchymal cell proliferation, initiating fracture repair, helping to form cartilage and intramembranous bone, and initiating callus formation...

Ngày tải lên: 11/08/2014, 05:20

... macromodeling and minimodeling continue in the adult skeleton, where macromodeling increases the ability of bone to resist bending (by expanding periosteal and endosteal cortices) and minimodeling rearranges ... sizes and shapes to bones Mostly independent resorption and formation modeling drifts it in bones and bone grafts Modeling drifts mainly determine outside bone diameter, cortical thickness, and ... deficits and age-associated decline in bone repair capacity Calcif Tissue Int 56(2), 123–129 130 Caplan, A I and Bruder, S P (1997) Cell and molecular engineering of bone regeneration, in Principles...

Ngày tải lên: 11/08/2014, 05:20

... healing of segmental bone defects induced by demineralized bone matrix J Bone Joint Surg 66, 274–279 79 Takagi, K and Urist, M R (1982) The role of bone marrow in bone morphogenetic protein-induced ... allograft incorporation In order to sustain osteogenesis, adequate cross-linking of collagen within the demineralized bone matrix must occur, and osteoinductive proteins must be present The source and ... host bone; and (7) it should possess desirable handling properties for the specific clinical indication (1,3,49) PRECLINICAL ANIMAL MODELS OF BONE REGENERATION One of the original preclinical...

Ngày tải lên: 11/08/2014, 05:20

... a single mutation in the proximal tyrosine kinase domain of FGFR3 (89) Interestingly, in the remaining individuals with hypochondroplasia, no mutations in FGFR3 were detected, despite screening ... tibial bone healing model This increase in bone was noted to originate from the existing bone surfaces (132) In contrast to PTH, which is produced in the parathyroid glands and is released into ... binds to the promoter of, and positively regulates, a variety of genes involved in bone formation, including these encoding osteocalcin, αI (1) procollagen, bone sialoprotein, and osteopontin...

Ngày tải lên: 11/08/2014, 05:20

... transplantation in vivo J Bone Miner Res 12, 1335–1347 78 Lane, J M., Yasko, A W., Tomin, E., et al (1999) Bone marrow and recombinant human bone morphogenetic protein-2 in osseous repair Clin Orthop ... percutaneous injections of bone marrow and demineralized bone matrix An experimental study in dogs Clin Orthop 268, 294–302 132 Tomford, W., Springfield, D S., and Mankin, H J (1992) Fresh and frozen ... morphogenetic protein-2-producing bone marrow cells on spinal fusion in rats J Bone Joint Surg 85A(5), 905–911 62 Alden, T D., et al (1999) Percutaneous spinal fusion using bone morphogenetic protein-2 gene...

Ngày tải lên: 11/08/2014, 05:20