1641 pardon of ste anne d auray

... turnover rate of HYDJs compared to HYDBp The pH and temperature dependence of HYDJs activity were measured (Fig 4) The results revealed an optimal temperature of HYDJs of 50 °C for hydrolyzing d, l-p-HPH, ... at which HYDJs hydrolyzes dihydrouracil Substrates Relative activity (%) Dihydrouracil Hydantoin D, L-p-hydroxyphenylhydantoin Dimethylhydantoin Phenylhydantoin Diphenylhydantoin 5-(hydroxymethyl)uracil ... BamHI and HindIII recognition sites The resulting PCR fragments were purified and double-digested using BamHI and HindIII, and cloned into pET28a(+) to generate pHYDs The pHYDs were introduced into...

Ngày tải lên: 18/02/2014, 08:20

... and 20 000 Da) Samples were loaded on a 10% acrylamide gel (D) Western blot analysis of purified PLDs using anti-(wild-type TH-2PLD) serum Lanes 1–3 contained lg of TH-2(H443A), TH-2F(H443A) and ... PLDs have been determined All Streptomyces PLDs except TH-2PLD have a Phe residue Sensor of phospholipids in PLD corresponding to Ala426 of TH-2PLD By contrast, at Lys438 of TH-2PLD, four of ... th-2pld) Then the amplified DNA fragments were cloned, sequenced and digested with PstI and NheI Next, the plasmids pETKmS2(G-F) and pETKmS2(G-FH) [14] were digested with NheI and BsiWI The two resulting...

Ngày tải lên: 19/02/2014, 02:20

... converted by a dehydratase to the corresponding 2-keto-3-deoxy sugar acid, which is then split by an aldolase to form pyruvate and d- glyceraldehyde or pyruvate and l-glyceraldehyde A glycerol dehydrogenase ... reading frame of gld2 coded for a protein with 318 amino acids and a calculated molecular mass of 35 663 Da The open reading frame for gld2 is deposited at GenBank and has the accession number DQ422038 ... nontagged protein, indicating that the tag did not affect the protein activity The tagged protein was then purified and further analysed The purified GLD1 showed activity with dl-glyceraldehyde and NADPH...

Ngày tải lên: 19/02/2014, 06:20

... mixture of a hexane/ethylacetate (1 : 1; v/v) The red-colored fraction (400–480 mL) was collected and concentrated under reduced pressure The residue was dissolved in 100 mL of chloroform Purification ... time of 2C-methylD -erythritol 2,4-cyclodiphosphate was 26 Fractions were combined and lyophilized Isolation of phytoene Fresh red peppers (107 g) were homogenized and lyophilized The dry powder ... modification of the method first described by Camara [35,36] Pericarp of red pepper (500 g) was homogenized at 8C in 600 mL of 50 mM Hepes, pH 8.0, containing mM dithioerythritol, mM EDTA and 0.4...

Ngày tải lên: 22/02/2014, 07:20

... mt9; 10, mt8; 11, GST of D0 2 was 4.0 nm and the KD of DNS 9.2 nm, that is double that of D0 2 This larger KD was accounted for by the twofold larger kd of DNS than that of D0 2 and their nearly similar ... of a degraded protein These results indicated that D0 2 NLS is mapped to the C-terminal 19 amino acids (288–306) encoded by exon Role of amino acid residues of a D0 2 NLS in nuclear import To determine ... GST-EGFP-DNS fusion protein as described above at the indicated temperature of 30 °C or °C and studied for GFP fluorescent signal and nuclear DNA Panels a and b, localization of DNS; panels c and d, ...

Ngày tải lên: 07/03/2014, 21:20

... 2 3 ND ND 1 3 3 ND ND Glycoform Relative abundanceb p 1209 1233 High Low Low Medium Medium – High – Medium Medium High High Medium Medium Medium Medium Medium Low Medium Medium Medium Medium ... High Medium Medium Medium Medium Medium Medium Medium Medium Medium Medium Fig ESI-MS2 analysis of permethylated dephosphorylated OS derived from LPS of NTHi isolate 1209 Product ion spectrum of ... and 13C NMR chemical shifts for Hep4-glycoforms of OS-1¢-B derived from LPS of NTHi isolate 1209 Data was recorded in D2 O at 22 °C Pairs of deoxyprotons of reduced AnKdo were identified in the DQF-COSY...

Ngày tải lên: 08/03/2014, 08:20

... low-dose, medium-dose and high-dose d- Gal groups was decreased by 1.8-fold, 2.5fold, and 4.8-fold, respectively; OGG1 expression was reduced by 2.4-fold, 3.2-fold, and 3.5-fold, respectively Decreased ... 1.4-fold, 1.7-fold, and 2.5-fold, respectively Decreased mRNA levels of DNA repair enzymes induced by D- Gal To investigate the effect of DNA repair enzymes on the mtDNA damage induced by d- Gal, ... development of presbycusis Results Age-related accumulation of the common deletion in mtDNA induced by D- Gal To evaluate the mtDNA damage induced by d- Gal, the percentage of the mitochondrial common deletion...

Ngày tải lên: 14/03/2014, 23:20

... coffin All eyes survey the record of name, of sex, of age, and the day of departure from earth records how shadowy! and dropped into darkness as if messages addressed to worms Almost at the very ... gave out a very different sound according to the varying slenderness or fulness of the current, devised an instrument that yielded a rude hydraulic gamut of sounds; and, indeed, upon this simple ... vanished all that I had relied on for protection: distance I had relied on, and suddenly I was found in close neighborhood to my most formidable enemy Poverty I had rolled on, and that was not denied:...

Ngày tải lên: 16/03/2014, 02:20

... I had heard of "the unpardonable sin," and well I recollect lying in my bed a mere child and having thoughts and words injected into my mind, which I [17]imagined were that sin, and shuddering, ... kind of pride and sadness; I had indeed the full measure of the New England boy's reserve in my early experience, and did not care whether others understood me or not And for a time something of ... had ever been a member of it It consisted of artists and other gentlemen, [86] an equal number of each Cole and Durand and Ingham and Inman and Chapman and Bryant and Verplanck and Charles Hoffman...

Ngày tải lên: 16/03/2014, 02:20

... spectra of 1-deoxy -D- xylulose 5-phosphate and its diastereomer Spectra were recorded in time intervals of days at 37 °C (A) Day (B) Day (C) Day (D) Day (E) Day The signals of [3,4,5-13C3]1-deoxy -D- xylulose ... removed by ultrafiltration (3 kDa cutoff) The solution was lyophilized The residue was dissolved in mL of water, and the pH was adjusted to Sodium metaperiodate was added to a final concentration of ... 6-phosphogluconate dehydrogenase, 62 U of glucose-6-phosphate dehydrogenase and 100 U of glutamate dehydrogenase were added The mixture was incubated at 37 °C, and the reaction was monitored by 13C-NMR...

Ngày tải lên: 16/03/2014, 06:20

... different steps in the AFI duplex of iso (G4C4) and the GG steps of iso BDNA: iso ADNA and ADNA Note the identical base stacking at the GG steps of AFI and ideal duplexes Figures were drawn using 3DNA ... 2¢,5¢ DNA and compact in 3¢,5¢ DNA [8,9] It is well known that the extended nucleotide repeats lead to an extended BDNA, and the compact nucleotide repeat leads to a compact ADNA type of duplexes ... 2¢,5¢ DNA fragment d( G4C4) is thus composed of extended nucleotide repeats that are normally part of BDNA but with a distinct X-displacement, slide and base stacking like in ADNA Thus, the duplex...

Ngày tải lên: 16/03/2014, 18:20

... The extracts were separated from cell debris, combined, dialyzed against distilled water and freeze-dried Descending chromatography and electrophoresis were performed on Filtrak FN-13 paper Electrophoresis ... analogous ester obtained upon alkaline hydrolysis of glucosylated ribitol teichoic acid from the cell wall of S azureus RIA 1009 [24] and based on the analysis of the products formed upon acid and enzymatic ... observed in the region of resonances of the CH3CO– groups The presence of 3-deoxynonulosonic acid with the b-configuration of the glycosidic bond followed from two doublets of doublets at d2 .20...

Ngày tải lên: 17/03/2014, 10:20

Ngày tải lên: 17/03/2014, 14:15

... and redox potentials of the free forms of wild-type and Y238 mutants of D- amino acid oxidase The semiquinone form of DAAO was achieved by anaerobic photoreduction, and the percentage of thermodynamically ... C Data were analysed as described by Minnaert [17] The amount of oxidized and reduced dye was determined at a wavelength at which the enzyme has no absorbance (> 550 nm) and the amount of oxidized ... of pkDAAO In fact, and different from the results obtained with RgDAAO mutants, both Y224F and Y228F mutants of pkDAAO showed a large decrease in kred (30- and 100-fold lower than in the wild-type)...

Ngày tải lên: 17/03/2014, 17:20

... products obtained by site-directed mutagenesis ND, not determined Enzyme Substrate VanXYCa D5 9S D5 9A VanXYCa D5 9S D5 9A D- Ala -D- Ala a D- Ala -D- Ala D- Ala -D- Ala UDP-MurNAc-pentapeptide[Ala] UDP-MurNAc-pentapeptide[Ala] ... and added to 750 lL of cadmium-ninhydrin stock solution; 70 lL of distilled water was added and incubated at 85 °C for The absorbance was measured at 505 nm and quantified with free amino acid ... (underlined) The product was purified, digested with BamHI and HindIII and cloned in pQE30 under the control of the T5 promoter Site-directed mutagenesis Mutants D5 9S and D5 9A were constructed...

Ngày tải lên: 18/03/2014, 01:20

Ngày tải lên: 18/03/2014, 15:21

... the A thaliana d- AAT D- Ala D- Asp D- Gln D- Asn D- Cys D- Met D- Thr D- Ser D- His D- Arg D- Lys D- Leu D- Val D- Tyr D- Phe D- Trp D- Pro 20 40 60 80 100 120 Relative activity (%) Fig D- Amino acid substrate specificity ... contents of other d- amino acids metabolically related to d- Asp, in particular d- Glu and d- Ala, were determined Plants cultured in MS medium, in the absence of d- Asp, showed no detectable levels of d- Glu ... Free d- amino acids and conjugated forms of d- amino acids have been detected in higher plants In the 1960s, N-malonyl -d- Trp was found in pea seedlings [32], and other conjugated d- amino acids have...

Ngày tải lên: 30/03/2014, 04:20

... Ireland -5.570754 (0.000)*** Dropped Dropped Belgium Dropped Portugal Dropped Dropped Denmark -2.199491 (0.003)*** Dropped Dropped Dropped Netherland Dropped Dropped Dropped Finland Dropped Dropped ... Dropped Dropped Dropped Italy Dropped Dropped Dropped Germany -27.2813 (0.238) Dropped 1.177698 (0.306) 0.0622312 (0.819) Dropped 2.681676 (0.150) Dropped -0.1902075 (0.6000) Dropped Dropped Japan ... funded R &D However, government direct R &D support crowded out the private R &D expenditure With 1% of government direct R &D support increases will leads to about 6% of business R &D expenditure decrease...

Ngày tải lên: 31/03/2014, 03:20

... associated to X and denoted Xred We say that X is reduced if X = Xred Irreducibility is another possible property of schemes; in spite of the name, it is independent of whether the scheme is reduced ... number of topics in classical geometry Thus for example we define blowups and study the blowup of the plane at various nonreduced points; and we define duals of plane curves, and study how the dual degenerates ... have been improved and extended, but the main difference is the addition of the material in Chapters IV and V, and related material elsewhere in the book These additions are intended to show schemes...

Ngày tải lên: 31/03/2014, 16:17

... available Some of these introduce VHDL before students get a good grasp of the fundamentals of digital design VHDL is a language that is used to describe the function of digital circuits/systems In ... circuit design and a discussion of the fundamental concepts of sequential circuits The complexity of modern digital systems is such that they have to be designed using computer-aided design (CAD) ... addition, we consider the octal and hexadecimal number systems and fixedand floating-point representation of numbers The chapter ends with a discussion on weighted and nonweighted binary encoding...

Ngày tải lên: 05/04/2014, 23:09