1 neural stem cell isolation and characterization

... Antibiotic B1 11.67 ± 1.25a 19.00 ± 0.82b 17.00 ± 0.00b 11.33 ± 1.25b - 16.67 ± 0.94c B2 12.67 ± 1.25a 14.00 ± 0.82b 14.70 ± 0.94b 10.67 ± 0.94a - 17.00 ± 0.00c B3 12.00 ± 0.82a 17.00 ± 0.00b 16.30 ... 0.47a - 11.67 ± 0.47b B9 12.33 ± 1.25a 16.00 ± 0.00b 15.67 ± 0.94b 11.00 ± 0.82a - 18.00 ± 0.00c B10 15.00 ± 0.82a 13.67 ± 0.94a 14.33 ± 0.47a 10.00 ± 0.82b - 9.33 ± 0.47b B11 13.67 ± 0.47a 17.67 ... 0.47b 16.67 ± 0.94b 13.00 ± 0.82a - 15.00 ± 0.00c B12 11.00 ± 0.82a 13.00 ± 0.82b 12.33 ± 0.94a 10.33 ± 0.47a - 11.33 ± 0.47a B13 15.00 ± 0.00a 15.33 ± 0.47a 15.33 ± 1.25a 12.33 ± 0.94b - 16.67...

Ngày tải lên: 09/01/2021, 17:57

... glia cells are neuronal progenitors in vivo 10 11 14 1.3 Long non-coding RNAs in biology 15 1.3.1 1.3.2 1.3.2.1 1.3.2.2 1.3.2.3 Long non-coding RNAs in pluripotency Long non-coding RNAs in neural ... ………………………………………………………… 1.1 Transcriptional control of stem cell pluripotency 1.1.1 The transcription factors OCT4, NANOG and SOX2 constitute the human embryonic stem cell transcriptional core 1.1.2 An expanded ... 1.1.3 Non-coding RNAs modulate pluripotency by regulating expression of the core transcription factors and/or downstream genes 1.2 Directed neural differentiation of hESCs 1.2.1 1.2.1.1 1.2.1.2...

Ngày tải lên: 09/09/2015, 17:54

... gametes Fig.1-1 Stem cell hierarchy in mouse (Adapted from Wobus & Boheler, 2005) Trang 16Chapter I Introduction 1.1.2 Fundamental features of stem cells Since stem cell renewal and differentiation ... as Stat1α and β, Stat3α, and β, Stat5A α and β, and Stat5B α and β (Schindler et al., 1992; Caldenhoven et al., 1996; Wang et al., 1996; Schaefer et al., 1997; 1996; Moriggl et al., 1996; Sasse ... 1.1 Stem cells 1.1.1 Developmental potency of stem cells Stem cells are unspecialized cells that have the capability of self-renewal for producing identical unspecialized daughter cells and the...

Ngày tải lên: 14/09/2015, 13:38

... chr6:14,388,338-14,393,355 3150 intergenic -1.15338 lncRNA_ES2 EF565083 chr1:198,709,840-198,710,182 343 intergenic -0.922722 lncRNA_ES3 BC026300 chr13:53,593,076-53,605,002 1053 intergenic 0.777772 * A ... AK056826 (lncRNA_ES1), EF565083 (lncRNA_ES2) and BC026300 (lncRNA_ES3) were specifically expressed in the three pluripotent stem cell lines (H1, H9 and iPS cell lines) Trang 19Figure 6.2: Pluripotent ... pluripotency and neural differentiation focused on the mouse as a model system (Dinger et al., 2008; Guttman et al., 2011; Mercer et al., 2010; Sheik Mohamed et al., 2010; Tochitani and Hayashizaki,...

Ngày tải lên: 09/09/2015, 17:54

... IDs: AK056164, AF429305 and AF429306 (Figure 7.1 and Figure 8.4) In humans, RMST is located on chromosome 12q23, and the ENCODE ChIP-seq data from human embryonic stem cells (H1) and neuroblastomas ... confirmed by qPCR in two independent cell lines: hESC (H1)-derived NPCs and an immortalized human neural stem cell line ReN-VM In both hESC-derived cells and ReN-VM cells, RMST expression was upregulated ... Pitx3-GFP- cell population (Uhde et al., 2010) In the developing mouse brain, Rmst expression in the ventral midbrain was high in the Lmx1a expressing region during E11.5 to E13.5 (Figure 8.1) Lmx1a...

Ngày tải lên: 09/09/2015, 17:54

... retina (Meola et al., 2012), apoptosis in erythroid differentiation (Hu et al., 2011), muscle differentiation (Cesana et al., 2011), retinal development (Rapicavoli et al., 2011) and in maintenance ... interactions (Fang et al., 2011), and this interaction was also observed in this study via the co-IP experiment hnRNPA2 and hnRNPB1 (referred to as hnRNPA2B1) are spliced isoforms, and are RNA-binding ... Telencephalon development GO:0021537 1.48E-04 8 Rho protein signal transduction GO:0007266 1.67E-04 10 Oligodendrocyte differentiation GO:0048709 1.89E-04 The top 10 terms, and their respective p-values...

Ngày tải lên: 09/09/2015, 17:55

... 18 AJ011955 Mala s 17 AJ011956 Mala s AJ011957 Mala s 19 AJ011958 Mala s 37 AJ011959 Mala s 10 heat shock prot. 70 86 AJ428052 Mala s 11 Mn superoxide dismut. 23 AJ548421 ... Mala s 1 X96486 Mala s 5 18 AJ 011 955 Mala s 6 17 AJ 011 956 Mala s 7 AJ 011 957 Mala s 8 19 AJ 011 958 Mala s 9 37 AJ 011 959 Mala s 10 heat shock prot 70 86 AJ428052 Mala s 11 Mn superoxide... ... 10 5 Asp... X78228 Cla h 12 acid ribosomal prot P1 11 X8 518 0 alkaline serine protease 34 Cladosporium herbarum AY1 918 16 Aspergillus flavus Asp fl 13 Aspergillus fumigatus Asp f 1 18...

Ngày tải lên: 14/09/2015, 08:47

... immunofluorescence 3.16 Cloning 3.16.1 Preparation of competent cells 3.16.2 Transformation 3.17 Cell synchronization studies 3.18 Overexpression and RNA interference studies 3.18.1 Overexpression 3.18.2 ... generation and verification 4.9 Subcellular localization of MOST-1 4.10 Cell synchronization studies Trang 64.12 Overexpression and RNA interference studies 110 Aggregation and implication of MOST-1 ... hybrids 10421 Alignment of Y2H screen interactors 105 23 RT-PCR analysis of various cell lines subjected to overexpression and RNAi experiments 111 24 Conclusion of MOST-1 characterization 137 Trang...

Ngày tải lên: 16/09/2015, 17:13

... Trang 1ISOLATION AND CHARACTERIZATION OF A C-REPEAT BINDING FACTOR GENE FROM TEVANG-1 MAIZE CULTIVAR Nguyen Thuy Linh 1 , Luu Han Ly 1 , Nguyen Thuy Duong 1,2 , Huynh Thi Thu Hue 1,2,* 1 Institute ... Academia Journal of Biology, 41(3): 85–100 https://doi.org/10.15625/2615-0923/v41n3.13872 *Corresponding author email: hthue@igr.ac.vn ©2019 Vietnam Academy of Science and Technology (VAST) Trang ... high-level expression of cor 6.6, cor15a, cor47, and cor78 (Kasuga et al., 1999) Meanwhile, CBF3 induced the transcription of rd29A, rd17, cor6.6, cor15a, erd10, and kin1 and biochemical changes related...

Ngày tải lên: 14/01/2020, 00:41

... (2017) 17:803 DOI 10.1186/s12885-017-3812-5 RESEARCH ARTICLE Open Access Isolation and characterization of a new naturally immortalized human breast carcinoma cell line, KAIMRC1 Rizwan Ali1,2, ... cancer stem cells and capability of producing mammosphere in-vitro was identified in both the cell lines On the other hand, three Chinese breast cancer cell lines, namely, BC-019, BC-020 and BC-021 ... epithelial cell markers They are cuboidal in shape and relatively smaller in size as compared to established cell lines, MCF-7, MDA MB-231 and the normal breast cell line, MCF-10A In normal cell culture...

Ngày tải lên: 06/08/2020, 03:27

... probably, cancer stem cells, such as CD44, Musashi-1 (MSI-1), CD133, CD166, DCAMKL-1 (doublecortin and calcium/calmodu-lin-dependent protein kinase-like-1), ALDH-1 (aldehyde dehydrogenase 1), LRIG (leucine-rich ... putative stem cell marker in the intestine of mice and humans and target gene of Wnt On the contrary, genes of other proposed stem cell markers, such as Dcamkl-1, Msi-1 and Prom-1 (CD133), were ... expression of two additional stem cell markers of colonic stem cells, i.e Musashi-1 (MSI-1) and doublecortin and calcium/calmodulin-dependent pro-tein kinase-like-1 (DCAMKL-1) MSI-1, firstly identified...

Ngày tải lên: 05/11/2020, 07:05

... 263–265 p107, 18 3, 18 5, 18 7 19 2, 19 4 19 6, 19 8, 19 9, 205– 210 , 232 p130, 15 8, 16 1, 16 2, 18 3, 18 7 19 2, 19 4 19 6, 19 8, 19 9, 205– 210 , 232 p14ARF , 259, 263, 265 p15INK4B , 2 41 p16INK4A , 18 4, 19 0, 19 2 19 4, ... knockouts, 12 – Cdk6 knockouts, 12 – S phase, 15 9, 16 8 Cdk inhibitor (CKI), 15 9, 16 1, 16 2, 16 6, 16 8, 230 Cdk1, 14 9, 15 9, 16 1, 16 4, 16 5 Cdk2, 15 3, 15 9, 16 4, 16 5, 18 4, 18 5, 18 7, 18 8, 19 3, 19 6, 208, ... 19 2 19 4, 19 6, 19 7, 19 9, 240, 2 41, 285, 290, 309 p18INK4C , 2 41 p19ARF , 19 3 19 9 p19INK4D , 2 41 p 21, 15 8, 16 1, 16 2, 16 6, 2 41, 333, 335 p27, 10 , 15 8, 16 1, 16 2, 2 41, 2 71, 273, 282, 286, 289, 293–3 01, ...

Ngày tải lên: 25/10/2013, 21:20

... American October 19 96 Copyright 19 96 Scientific American, Inc Electronic Publishing Martin Paul, DIRECTOR Scientific American, Inc 415 Madison Avenue New York, NY 10 017 -11 11 ( 212 ) 754-0550 PRINTED ... inFriction at the Atomic Scale 10 10 10 10 10 SHEAR STRESS (NEWTONS PER SQUARE METER) 10 10 10 10 struments Collectively, they have recorded a range of stress spanning 12 orders of magnitude, all ... American, Box 318 7, Harlan, Iowa 515 37 Reprints available: write Reprint Department, Scientific American, Inc., 415 Madison Avenue, New York, N.Y 10 017 -11 11; fax: ( 212 ) 355-0408 or send e-mail to...

Ngày tải lên: 12/05/2014, 15:15

... paclitaxel, we treated 1A9 and PTX10 cells with DMSO (control) and 10 , 25, 50, 75, and 10 0 nM of VE-465 in the presence of 15 ng/mL paclitaxel for 96 hours In the parental 1A9 cell line, paclitaxel ... for 1A9 and PTX10 cell lines, and Merck & Co and Vertex 19 Weaver BA, Cleveland DW: Decoding the links between mitosis, cancer, and chemotherapy: The mitotic checkpoint, adaptation, and cell ... genes to be Affymetrix Probe ID Gene Name 3 910 9_at 11 25_s_at; 11 26_s_at 36863_at 3 215 7_at 40757_at 19 85_s_at 38370_at TPX2 CD44 HMMR PPP1CA GZMA NME1 TIAM1 TPX2, microtubule associated, homolog CD44...

Ngày tải lên: 18/06/2014, 15:20

... stem- cell biology to cancer Nat Rev Cancer 2003, 3:895-902 Reya T, Morrison SJ, Clarke MF, Weissman IL: Stem cells, cancer, and cancer stem cells Nature 20 01, 414 :10 5 -11 1 15 16 17 18 19 20 21 ... cancer cells MDA-MB-2 31 by genistein Oncogene 19 99, 18 : 316 6- 317 2 Davis JN, Kucuk O, Sarkar FH: Genistein inhibits NF-kappa B activation in prostate cancer cells Nutr Cancer 19 99, 35 :16 7 -17 4 Wu ... 2008, 1: 9 http://www.ovarianresearch.com/content /1/ 1/9 Figure The expression of c-IAP1, Bcl-2, Bcl-xl, survivin and PARP in A2780 and C200 cells The expression of c-IAP1, Bcl-2, Bcl-xl, survivin and...

Ngày tải lên: 20/06/2014, 07:20

... to cisplatin? Cell Cycle 2008, 7 :13 53 -13 59 Yallapu et al Journal of Ovarian Research 2 010 , 3 :11 http://www.ovarianresearch.com/content/3 /1/ 11 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 ... Research 2 010 , 3 :11 http://www.ovarianresearch.com/content/3 /1/ 11 Page of 12 where it binds with the TCF transcription factor and upregulates the expression of cell survival genes such as cMyc and c-Jun, ... labeled cellulose nanocrystals for bioimaging applications J Am Chem Soc 2007, 12 9 :13 810 -13 811 Gavert N, Ben-Ze'ev A: beta-Catenin signaling in biological control and cancer J Cell Biochem 2007, 10 2:820-828...

Ngày tải lên: 20/06/2014, 07:20

... Research 2 010 , 3 :13 http://www.ovarianresearch.com/content/3 /1/ 13 15 16 17 18 19 20 21 22 23 24 25 26 expressed in the normal human breast and in breast cancer Med Oncol 2004, 21: 123- 31 Wente W, ... Research 2 010 , 3 :13 http://www.ovarianresearch.com/content/3 /1/ 13 Page of 10 Figure Effect of the LXR agonist T09 013 17 on cellular morphology CaOV3 cells were cultured and treated with DMSO (1% , A) ... and collected and × 10 5 cells per sample were stained with 10 μl of FLICA reagent and 7-AAD and Rough et al Journal of Ovarian Research 2 010 , 3 :13 http://www.ovarianresearch.com/content/3 /1/ 13...

Ngày tải lên: 20/06/2014, 07:20

... markers and functional regulators of stem cells Stem Cells 2002, 20 :11 -20 Ponnusamy et al Journal of Ovarian Research 2 011 , 4:7 http://www.ovarianresearch.com/content/4 /1/ 7 Page 10 of 10 15 Kim ... cancer side population defines cells with stem cell- like characteristics and Mullerian Inhibiting Substance responsiveness Proc Natl Acad Sci USA 2006, 10 3 :11 154 -11 159 14 Bunting KD: ABC transporters ... signaling in polarized CACO-2 epithelial cells indicate that Muc4 acts as an unorthodox ligand for ErbB2 Mol Biol Cell 2006, 17 :29 31- 29 41 doi :10 .11 86 /17 57-2 215 -4-7 Cite this article as: Ponnusamy...

Ngày tải lên: 20/06/2014, 07:20

... SERPIND1 A2M MTMR 11 PSG 11 PAM NOS3 GAGE6 CLYBL GAGE7B SERPINE2 CECR5 ADAM15 DPYSL3 REG4 GALR2 TFF2 EEF1A2 PRSS3 GNG 11 CD96 LPXN SGK MLLT 11 CFB GADD45A MYH4 CXCL6 GABARAPL1 POU2F2 PRSS1 CYR 61 TNFRSF11B ... CLCA1 APOE PRSS3 C20orf75 RPIB9 APOA1 CCL26 RFTN1 MSMB CCL26 CCL26 PRSS2 WFS1 GNG 11 IL8 TXNIP GAGE6 XAGE1 TCN1 SCARF2 ANKRD38 CDH 11 PRSS1 RHOU MFGE8 CEACAM6 ABCB1 PRSS2 SCRG1 GAGE7B MAPK13 LDHA ... ECAT 11 SPP1 DDIT4L APOE SPOCK2 NINJ2 THBS1 SOX 21 CD44 RGS4 DDIT4 IGF2 GPC3 PRSS8 APOC1 ITIH2 MAF FABP5 IGSF4 SOX 21 NPC2 SCD MT1F RRAGD SPOCK2 RENBP SPINT2 RFTN1 TCN1 PRNP FKBP 11 MSMB LCP1 NNMT...

Ngày tải lên: 20/06/2014, 08:20

... .1 1 .1 GSCs and the GSC Niche in Drosophila Ovary 1. 1 .1 GSCs and Their Intrinsic Factors 1. 1 .1. 1 Drosophila GSCs 1. 1 .1. 2 Intrinsic Factors for GSC Maintenance 1. 1 .1. 3 ... 16 -cell cyst (Panels C and D in Figure 1. 1) Among the 16 -cell cyst, one cell cyst develops into an oocyte while the remaining 15 cell- cystocytes adopt the cell fate of nurse cells GSCs, CBs and ... 32 2 .1. 1 PCR, Quantitative Real-time PCR, Inverse PCR and Primers 32 2 .1. 1 .1 PCR .32 2 .1. 1.2 Quantitative Real-time PCR 33 2 .1. 1.3 Inverse PCR 33 2 .1. 1.4 PCR...

Ngày tải lên: 14/09/2015, 08:42