1 il 8 and vegf mrna expression in lt2 endothelial cells

... expression with shorter DFS, MFS and OS (Table 3) The values of DFS, MFS, and OS of patients with high CCR-2 expression were as fol-lows (months): 87.57 ± 18.57, 87.71 ± 18.58, and 114.67 ± 15.29, ... low/negative expression were 127.57 ± 8.42, 133.94 ± 7.52, and 140.44 ± 6.41, respectively (Fig 3 and Table 3) Association of expression in spindle-shaped stromal cells of OPG, TRAIL, RANKL, SDF-1, IL-6, ... positive cells were assigned scores as follows: 0 (<10%), 1 (10%– 30%), 2 (31%–60%), 3 (61%–90%), and 4 (>90%) Staining intensity was scored as 0 (no staining), 1 (weak), 2 (moder-ate), and...

Ngày tải lên: 19/09/2020, 21:50

... changes in expression of C integrin αα2 paxillin merged (+TO-PRO-3) A2780 Vector A2780 PRL-3 KD-22 A2780 PRL-3 KD-3 D integrin α2 paxillin paxillin (pY195) paxillin (pY141) paxillin (pS178) integrin ... (pT183/pY185), p38, phos-pho-p38 (pT180/pY182) were from BD Biosciences An-tibodies against paxillin and phospho-paxillin (pY195), phospho-paxillin (pY141), and phospho-paxillin (pS178) were from ... integrin β1 integrin αV GAPDH integrin α2 paxillin integrin β1 GAPDH Figure 4 Knock-down of endogenous PRL-3 expression in A2780 cells upregulates expression of integrin α2 and paxillin (A) Lysates...

Ngày tải lên: 05/11/2020, 07:12

... protein expression in Ishikawa cells after genistein and E 2 treatment Genistein + E 2 -H 40×10-6+10-6 0.1152(0.0155) * 0.2608(0.0217)* Genistein + E 2 -L 40×10-6+10-8 0.9417(0.0528) # 0.9116(0.0413)# ... activity (Intensity/A 420 ) Control 0 4110 (83.90) 3066(46.21) E2-H 10-6 12367(817.8)* 10472(43.32)* E2-L 10-8 22562(469.4)* 15119(236.6)* Genistein 40×10-6 9970.9(552.8)* 13565.5(516.6)* Note: ... Concentration (mol/L) Change in luciferase activity E2-H 10-6 8780(816.8)* 6894(13.44)*# E2-L 10-8 18965(469.4)* 11530(235.6)*# Genistein 40×10-6 6387.5(752.9)*▲ 9982.3(1580.4)*▲# Note: All data...

Ngày tải lên: 04/12/2022, 10:39

... (GAPDH) (p7 and p8), E-selectin (p9 and Trang 4p10), a1,3GalT transcripts 5¢-A (p11 and p12), 5¢-B (p13and p14), 5¢-C (p15 and p16), and total amount of pig a1,3GalT mRNA (p17 and p18) were used ... is endothelial cell-specific in vitro and in vivo and contains critical Sp1 and GATA binding sites J Biol Chem 273, 11737– 11744. 37 Iljin, K., Dube, A., Kontusaari, S., Korhonen, J., Lahtinen, ... C.3 and C.5 were only tested in COS7. A 6 3 1.5 0.5 Size in kb 1 2 3 4 5 B GATA GATA GATA Bgl II Koike et al [21] Ap-1 GATA BamH I Oct-1 Koike et al [21] Sp1 Sp1 Sp1 Sp1 Sp1 Sp1 Sp1 AhR/Arnt...

Ngày tải lên: 08/03/2014, 16:20

... Sphk1, Ereg, Vegfa, Lect1, Col18a1 and Il6; c) Ereg, Ephb4 and Vegfa; d) Tbx1, Cxcl5, Col4a3, Col18a1, F2, Vegfc, Sphk1, Ccl11, S1pr1, F2, Il6, Lect1, Flt1 and Csf3 e) Sphk1 and Vegfa; f) Csf3 ... molecule CD31 (PECAM-1) and negative for leukocyte markers CD11b and CD45 (Fig-ure 3A-D) As expected, the endothelial cells uniformly expressed the surface molecules VEGFR1 (Flt1) and CD31(Pecam1) (Figure ... significant increase in expression on Day 6 (6- to 30-fold) Interest-ingly, Vegfa expression continued to rise slightly until Day 14–resulting in a slightly misleading temporal clus-tergram Vegfa has...

Ngày tải lên: 12/08/2014, 13:22

... reported in urine by ELISA and Western blot [15,16] and HIV-1 DNA has been detected in urine pellets from HIV-1 infected individuals [17,18] However, it is not clear whether urine from chronically infected ... recruited in 2008 (Table 1) Group A: 10 HIV-1 negative individuals; Group B: 11 HIV-1 infected individuals, who are drug nạve with detectable viral load in plasma ranging from 428 to 78,636 and CD4+ ... T cells, which could be the result from HIV direct kill-ing of CD4-expresskill-ing primarily infected cells, HIV indi-rect killing of bystander cells through HIV proteins and/or by the proinflammatory...

Ngày tải lên: 10/08/2014, 05:21

... 58(6):1810-1822. doi:10.1186/1465-9921-11-1 Cite this article as: Velosa et al.: Collagen V-induced nasal tolerance downregulates pulmonary collagen mRNA gene and TGF-beta expression in experimental ... nucleator and point sampled intercepts and their use in pathological research and diagnosis APMIS 1988, 96(10):857-881. 20 Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time ... reduction in total collagen content around the vessels (0.371 ± 0.118 vs 0.874 ± 0.282, p < 0.001), bronchioles (0.294 ± 0.139 vs 0.646 ± 0.172, p < 0.001) and in the septal interstitium...

Ngày tải lên: 12/08/2014, 14:20

... logFccontrol-treated) and TIMP1 was also upregu-lated in 5-Aza treated SW480 cells according to GSE14526 Among the 5 CRC cell lines of GSE32323 SULT1A1 in Colo32 cells, PTGS2 in HCT-116 cells, ALDH1A3 and SOCS3 in ... varying degrees of upregulation of cer-tain transcripts In HT-29 cell line ALDH1A3 and SOCS3 was found to be upregulated by 0.5 μM 5-Aza Interestingly, in HCT-116 cells PTGS2; and in SW480 cell ... GSE4183 (8 normal, 15 ad-enoma and 15 CRC), GSE10714 (3 normal, 5 adad-enoma and 7 CRC), GSE37364 (38 normal, 29 adenoma and 27 CRC)) and GSE15960 (laser microdissected colonic epithelial cells...

Ngày tải lên: 22/09/2020, 23:39

... for 10 min All amplified prod-ucts were separated in 1 % agarose gels, and the bands Trang 8Page 8 of 10Su et al Cancer Cell Int (2016) 16:50 were visualized by ethidium bromide staining In order ... Luteinizing hor-mone increases human endometrial cancer cells invasiveness through activation of protein kinase A Cancer Res 2003;63:4281–6. Trang 10Page 10 of 10Su et al Cancer Cell Int (2016) 16:50 ... Trang 1PRIMARY RESEARCHInvolvement of human chorionic gonadotropin in regulating vasculogenic mimicry and hypoxia-inducible factor-1α expression in ovarian cancer cells Min Su1†, Xiangxiang Xu1,2†,...

Ngày tải lên: 04/12/2022, 14:54

... phosphatidylinositol A B P-Erk1/2 P-p38 + S1P + Anisomycin + Serum P-JNK1/2 P-Akt Total-Erk1/2 0 5 10 15 20 0 5 10 15 20 0 5 10 15 20 min 0 100 200 300 400 500 600 Control S1P Anisomycin Serum ... * No Inhibitor + Pd-098059 + SB-203580 + SP-600125 + Wortmanin No Inhibitor + Pd-098059 + SB-203580 + SP-600125 + Wortmanin Fig 7 Effects of MAP kinase and PtdIns 3-kinase inhibitors on S1P-, ... pretreated for 30 min with Pd-098059 (20 lM), SB-20856 (10 lM), SP-600125 (10 lM), or worthmanin (10 lM), which inhibit Erk1/2, p38, JNK1/2, and PtdIns 3-kinase, respectively These inhibitors were...

Ngày tải lên: 19/02/2014, 16:20

... protein; GST, glutathione S-transferase; HD, Huntington’s disease; HIP1, Huntingtin interacting protein 1; HIPPI, Huntingtin interacting protein 1 protein interactor; Htt, Huntingtin; IOD, integrated ... AAATACATG 8.00E+007 6.00E+007 4.00E+007 2.00E+007 0.00E+000 14 16 12 10 8 6 4 0.00 0.01 0.02 0.03 0.04 0.05 0.00 0.02 0.04 0.06 [DNA] µM [DNA] µM 1.50E-008 1.40E-008 1.28E-008 1.26E-008 0.105 0.100 ... 0.085 0.080 1.20E-008 1.10E-008 1.00E-008 F 340 F 340 0 25 50 75 100 1/[DNA] µM 20 25 30 35 40 45 50 1/[DNA] µM 0 11 22 33 44 55 1/[DNA] µM 0.08 Probe (9 bp) AAAGACATG II I I shifted band Fig 1...

Ngày tải lên: 07/03/2014, 10:20

... additional zinc-binding domains (B-box), and a putative leucine coiled-coil region The RING finger coordinates two zinc atoms and is found almost exclusively in the N-terminal position in RBCC proteins ... Trang 1RING finger, B-box, and coiled-coil (RBCC) protein expressionKentaro Miyamoto1, Nobuhiro Nakamura1, Masahide Kashiwagi1, Shinji Honda1, Akira Kato1, Sanae Hasegawa2, Yoshio Takei2and Shigehisa ... membrane was rinsed twice in 2· NaCl/Cit (1 · NaCl/Cit contains 0.15 mMNaCl and 0.015Msodium citrate) containing 0.1% SDS for 30 min at 50C, washed with 0.5 · NaCl/Cit containing 0.1% SDS for 1 h at...

Ngày tải lên: 17/03/2014, 10:20

... (TMEV) infection of brain endothelial cells using in vitro and in vivo approaches Methods: i) in vitro: VCAM-1 was measured by ELISA in supernatants of brain endothelial cells infected with TMEV and ... AEA in regulating 1) VCAM-1 expression in brain endothelial cells infected with TMEV and the possible receptors involved by using antagonists of the classical cannabinoid receptors, CB1 and CB2, ... [57] In this line, AEA enhances IL-6 production in astrocytes infected with TMEV by a CB1receptor-mediated pathway [58] and in a more recent work AEA modulates TMEV-induced IL-12, IL-23 and IL-10...

Ngày tải lên: 19/06/2014, 22:20

... Journal of Inflammation 2006, 3 :8 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 Gentili C, Morelli S, Russo De Boland A: Involvement of PI3kinase and its association with c-Src in PTH-stimulated ... Sci U S A 012 5, 98 :13 6 81 - 13 686 Hiller G, Sternby M, Sundler R, Wijkander J: Phosphatidylinositol 3-kinase in zymosan- and bacteria-induced signalling to mobilisation of arachidonic acid in macrophages ... Longnecker R, Kinet JP, Witte ON, Scharenberg AM, Rawlings DJ: Btk/Tec kinases regulate sustained increases in intracellular Ca2+ following B-cell receptor activation Embo J 19 98, 17 :19 73 -19 85 Saito...

Ngày tải lên: 11/08/2014, 08:21

... seedlings seedlings (S/D) T1 -1 31 16 15 1. 1 :1 T1-2 23 17 2 .8 :1 T1-3 27 24 8 :1 pG2NBL- T1-4 31 28 9.3 :1 UAS::STM T1-5 26 26 T1-6 27 19 2.4 :1 T1-7 18 14 3.5 :1 T1 -8 29 24 4 .8 :1 T1-9 31 29 14 .5 :1 T1 -10 ... seedlings seedlings (S/D) T1 -1 31 25 4.2 :1 T1-2 28 23 4.6 :1 T1-3 32 19 13 1. 5 :1 pG2NBL- T1-4 37 28 3 .1: 1 UAS::CLE40 T1-5 25 21 5.3 :1 T1-6 31 24 3.4 :1 T1-7 32 18 14 1. 3 :1 T1 -8 31 23 2.9 :1 32  ... seedlings seedlings (S/D) T1 -1 24 20 5 :1 T1-2 42 40 20 :1 T1-3 35 34 1: 34 pG2NBL- T1-4 27 27 UAS::WUS T1-5 25 18 T1-6 32 32 T1-7 29 19 10 1. 9 :1 T1 -8 29 28 28 :1 T1-9 31 26 5.2 :1 T1 -10 24 24 T1 -11 ...

Ngày tải lên: 26/09/2015, 09:47

... p 413 GAL1-PKR) or cotransformed with p426GAL1-PKR and p425GAL1-Nck -1 or p 413 GAL1-PKR and p425GAL1-Nck -1 using lithium acetate [37] Transformants were selected and maintained in synthetic minimal ... arrest and DNA damage-inducible protein GADD34 assembles a novel signaling complex containing protein phosphatase and inhibitor Mol Cell Biol 21, 68 41 685 0 16 He B, Gross M & Roizman B (19 98) The ... Pi and lysed in ice-cold lysis buffer containing 10 mm Tris ⁄ HCl (pH 7.4), 50 mm KCl, mm MgCl2, 1% Triton X -10 0, lgÆmL )1 aprotinin, lgÆmL )1 leupeptin, mm dithiothreitol, 0 .1 mm Na2VO4 and 0.1...

Ngày tải lên: 07/03/2014, 05:20

... TGF- 1 affects ASMC proliferation In human ASMCs, it was found that TGF- 1 induced a 10 –20 fold increase in insulin-like growth factor binding protein-3 (IGFBP-3) mRNA and protein and a 2-fold increase ... cell counting As shown in Figure 1, increases in [3H]-thymidine incorporation occurred in all three conditions, but TGF 1 and 10 % FBS induced the strongest response in ASMCs cultured in 0.2% BSA/DMEM ... MEK in human ASMCs [ 31] In addition, inhibition of MEK attenuates mitogen-induced increase in promoter activity, mRNA or protein of cyclin D1 or c-Fos [30,32, 38] However, the role of p 38 and...

Ngày tải lên: 12/08/2014, 16:20

... Rupture is Kept from Coming Out page 12 16 20 The Care and Attention We Give You Able to Work While Being Cured Don't Let Yourself be Scared into Risking an Operation Why Ordinary Trusses Do More ... the Cluthe Rupture Institute, made his start in life in the Surgical Instrument business Learned his trade in the old country —over in Germany, where the world's finest surgical instruments are ... against the abdominal wall—any one, whether ruptured or not, can plainly feel that pressure when coughing or sneezing; while lifting or other exertion greatly increases the pressure or strain...

Ngày tải lên: 06/03/2014, 15:20

... 74 76 81 81 84 86 91 93 93 95 10 2 10 4 10 7 11 1 11 5 12 1 12 1 12 4 12 8 13 2 13 8 14 1 14 3 15 3 15 3 16 1 16 4 16 8 17 4 17 8 Chapter THE SYSTEM OF REAL NUMBERS 1 Rational and Irrational ... of points 1 1 /8 2n | | | The set of intervals 1 0, 1/ 4 1/ 2 | 1, Fig | | | | 1 , , 21 2n 1 , n | | | | covers the interval 1 1, because every point of 1 is a point of one of the intervals ... One ordinarily √ says that is 1. 4+, or 1. 41+ , or 1. 414 +, etc The exact meaning of these statements is expressed by the following inequalities:2 (1. 4)2 < < (1. 5)2 , (1. 41) 2 < < (1. 42)2 , (1. 414 )2...

Ngày tải lên: 28/06/2014, 19:20

... "4 "8& "%" 17   , 7+ *8 + 8 &'+&9+ 8& : + *8 '+ 8 &'+9* *8 : +99&:7 &)*(&'*9&) 8 ))(*)&()* *;*'*%'&;& 8& )(*'')*&&*(*(( ... *'9&*(%(*&*&+(''*)

Ngày tải lên: 05/11/2014, 12:32